FastQCFastQC Report
Fri 27 May 2016
SRR2140373_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140373_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9348973
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA156450.056.009471
GTATCAA302100.039.2176631
ATCAACG370800.031.5155563
TCAACGC370900.031.4956194
TATCAAC382600.030.7110732
CAACGCA381050.030.6789465
AACGCAG392950.029.7489176
TGGTATC60600.029.0597922
GTGGTAT67900.027.1229531
ACGCAGA451150.025.9008227
CGCAGAG457600.025.5357428
GCAGAGT524100.022.3442369
GTACATG405900.021.2432861
GAGTACT290450.021.04323412-13
TACATGG403100.020.811862
ACATGGG413200.019.7273083
CAGAGTA516550.019.66447410-11
GTACTTT320250.019.01947614-15
AGAGTAC491400.018.55114710-11
AGTACTT312350.017.5030412-13
Produced by FastQC (version 0.11.5)