FastQCFastQC Report
Fri 27 May 2016
SRR2140372_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140372_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9845629
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA137050.052.8143541
GTATCAA261100.040.3154371
TCAACGC325650.031.6408184
ATCAACG330700.031.1576423
TATCAAC337650.030.6947652
CAACGCA337050.030.5833055
AACGCAG348650.029.6625376
ACGCAGA398500.025.9397647
CGCAGAG405700.025.4583598
TGGTATC60500.023.5750392
GTGGTAT63350.023.1885241
GCAGAGT467450.022.0496489
GTACATG346000.021.326961
CTTATAC65100.020.9908641
TACATGG345700.020.8637122
GAGTACT269550.020.30711612-13
ACATGGG348000.020.135893
AGAGTAC428100.019.2242210-11
CAGAGTA455300.019.20577610-11
GTACTTT291450.018.65767914-15
Produced by FastQC (version 0.11.5)