FastQCFastQC Report
Fri 27 May 2016
SRR2140371_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140371_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9810555
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA142900.055.2937851
CTTATAC87200.041.5250051
GTATCAA278700.040.503911
TATACAC119300.033.3104863
TCAACGC352550.031.5591644
ATCAACG356400.031.2182463
TATCAAC361350.030.9570242
CAACGCA363700.030.6033485
AACGCAG371800.030.0036076
TTATACA130100.029.3694422
ACGCAGA423750.026.3537987
CGCAGAG431000.025.9104928
TACACAT163650.023.9711825
ACACATC154300.023.6576466
ACATCTC157400.023.4065158
GCAGAGT496250.022.4436119
GTACATG376700.022.1615451
GTGGTAT63900.022.1072061
TGGTATC61950.021.8415012
TACATGG373750.021.72172
Produced by FastQC (version 0.11.5)