FastQCFastQC Report
Fri 27 May 2016
SRR2140370_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140370_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9955300
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA142200.051.746511
GTATCAA266500.039.781651
TCAACGC323900.032.0478324
ATCAACG326950.031.7618713
TATCAAC335300.031.2512192
CAACGCA333850.031.0544875
AACGCAG343300.030.2233546
ACGCAGA398650.026.0150647
CGCAGAG404800.025.5988278
GTGGTAT64050.024.7555891
TGGTATC63600.023.7276552
GCAGAGT470050.022.0995799
GTACATG330900.021.4023671
TACATGG334700.020.6640472
CTTATAC98100.020.4096451
GAGTACT280950.020.37061912-13
ACATGGG340250.019.663943
CAGAGTA457150.019.21337110-11
AGAGTAC430600.018.60154310-11
GTACTTT310250.018.24958814-15
Produced by FastQC (version 0.11.5)