##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140370_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9955300
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.38258435205368	34.0	31.0	34.0	31.0	34.0
2	32.47205508623547	34.0	31.0	34.0	31.0	34.0
3	32.524386407240364	34.0	31.0	34.0	31.0	34.0
4	36.0503642280996	37.0	35.0	37.0	35.0	37.0
5	36.09656594979559	37.0	35.0	37.0	35.0	37.0
6	36.08592006268018	37.0	35.0	37.0	35.0	37.0
7	36.053367653410746	37.0	35.0	37.0	35.0	37.0
8	36.03684369130011	37.0	35.0	37.0	35.0	37.0
9	37.77835364077426	39.0	38.0	39.0	35.0	39.0
10-11	37.71603788936547	39.0	37.0	39.0	35.0	39.0
12-13	37.7048150231535	39.0	37.0	39.0	35.0	39.0
14-15	39.04610222695449	40.0	38.0	41.0	36.0	41.0
16-17	39.05977132783542	40.0	38.0	41.0	36.0	41.0
18-19	39.1062061916768	40.0	38.5	41.0	36.0	41.0
20-21	39.133233976519264	40.0	39.0	41.0	36.0	41.0
22-23	39.0943908189849	40.0	39.0	41.0	36.0	41.0
24-25	39.05963089800488	40.0	39.0	41.0	36.0	41.0
26-27	39.00242079435962	40.0	39.0	41.0	35.0	41.0
28-29	38.96382134962737	40.0	38.0	41.0	35.0	41.0
30-31	38.88511962987131	40.0	38.0	41.0	35.0	41.0
32-33	38.782673190953524	40.0	38.0	41.0	35.0	41.0
34-35	38.71512587197546	40.0	38.0	41.0	35.0	41.0
36-37	38.668838300421754	40.0	38.0	41.0	35.0	41.0
38-39	38.626515998045825	40.0	38.0	41.0	35.0	41.0
40-41	38.57628498427346	40.0	38.0	41.0	34.5	41.0
42-43	38.51365016991291	40.0	38.0	41.0	34.0	41.0
44-45	38.47439922401581	40.0	38.0	41.0	34.0	41.0
46-47	38.39901371528977	40.0	38.0	41.0	34.0	41.0
48-49	38.33288318207475	40.0	38.0	41.0	34.0	41.0
50-51	38.25431539307675	40.0	38.0	41.0	34.0	41.0
52-53	38.145604756638434	40.0	37.0	41.0	33.5	41.0
54-55	38.03236685009253	40.0	37.0	41.0	33.0	41.0
56-57	37.889902536879916	40.0	37.0	41.0	33.0	41.0
58-59	37.74215661097041	39.0	36.0	41.0	33.0	41.0
60-61	37.56356818714268	39.0	36.0	41.0	33.0	41.0
62-63	37.34834789457323	39.0	35.5	41.0	32.5	41.0
64-65	37.11696087956914	38.5	35.0	40.0	32.0	41.0
66-67	36.83926371770117	38.0	35.0	40.0	32.0	41.0
68-69	36.5676913913667	37.0	35.0	40.0	32.0	41.0
70-71	36.25717563197625	37.0	35.0	39.5	31.0	41.0
72-73	35.928611205766856	36.5	35.0	39.0	31.0	41.0
74-75	35.605457688282264	36.0	34.5	39.0	31.0	40.0
76-77	35.276931638462045	35.5	34.0	37.5	31.0	40.0
78-79	34.980876540643436	35.0	34.0	37.0	31.0	39.0
80-81	34.697991843661036	35.0	34.0	37.0	31.0	39.0
82-83	34.422020303210196	35.0	34.0	36.0	31.0	38.5
84-85	34.1929864094039	35.0	34.0	36.0	30.0	37.0
86-87	33.999359148479414	35.0	34.0	35.5	30.0	37.0
88-89	33.859567271404806	35.0	34.0	35.0	30.0	36.5
90-91	33.76343185763953	35.0	34.0	35.0	30.0	36.0
92-93	33.6829858775216	35.0	34.0	35.0	30.0	36.0
94-95	33.59567461591372	35.0	34.0	35.0	30.0	36.0
96-97	33.60097118322537	35.0	34.0	35.0	30.0	35.5
98-99	33.68806502306695	35.0	34.0	35.0	31.0	35.0
100	32.895103397586745	34.0	32.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	5.0
19	25.0
20	76.0
21	285.0
22	1025.0
23	3237.0
24	8052.0
25	17477.0
26	31902.0
27	52432.0
28	78257.0
29	107419.0
30	142810.0
31	184864.0
32	237978.0
33	313063.0
34	439235.0
35	648691.0
36	1098640.0
37	2180767.0
38	2913395.0
39	1451808.0
40	43857.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.240916898536454	20.139212278886625	14.26009261398451	24.35977820859241
2	14.353664351901383	20.979620964341887	41.73311960705546	22.933595076701273
3	17.96925797392082	26.319154516434818	30.240735158378502	25.470852351265865
4	12.41592165297312	18.3953207628742	38.577509181543334	30.61124840260934
5	13.433985940900673	37.60109912430484	35.11491000251737	13.850004932277123
6	29.55239569425675	37.859857915736505	18.863880398565634	13.723865991441112
7	26.387621137820854	32.8272608350151	22.834224969571462	17.95089305759258
8	24.428053398692153	36.54945606862676	21.094201078822337	17.92828945385875
9	25.697166333510797	17.048476690807913	21.14583186845198	36.10852510722932
10-11	24.193560214157284	26.891037939589967	28.507473406125385	20.407928440127368
12-13	25.230033248621336	24.393142346287906	28.66255662812773	21.714267776963027
14-15	22.363535001456512	25.57983184836218	26.55983245105622	25.49680069912509
16-17	21.723910881640933	28.76109710405513	27.200636846704768	22.314355167599167
18-19	21.740716000522333	27.647017166735306	29.217100438962163	21.395166393780197
20-21	22.586898602203817	26.728407541002298	29.152801255477584	21.5318926013163
22-23	22.292431040921382	26.84282450936629	28.971458304260363	21.893286145451963
24-25	22.072551284720355	27.00775258773438	29.06087504207846	21.858821085466804
26-27	22.008400507385943	27.158266284936	29.002388103602065	21.83094510407599
28-29	21.898882572446926	27.006214032505195	29.081247690506906	22.01365570454098
30-31	22.33553965765086	26.918227379150927	28.96195887927559	21.784274083922625
32-33	22.09258802519838	27.076144733262236	28.974591107583976	21.856676133955407
34-35	22.191827097919287	27.11910608412267	28.946079018001292	21.742987799956754
36-37	22.185661269365035	26.97710600639645	28.87144182696743	21.965790897271084
38-39	21.933298740516268	27.051627224860397	28.99419306634055	22.02088096828279
40-41	22.24021469732638	26.911677479209324	28.871471514684966	21.976636308779334
42-43	22.19725099510786	26.969909513044666	28.652284137742406	22.180555354105067
44-45	22.1964789034914	27.21244906339995	28.388865424109937	22.202206608998708
46-47	22.424753367206947	27.173072272042226	27.93625304033467	22.465921320416157
48-49	22.346726323699514	27.27943166993107	27.86764836440556	22.50619364196385
50-51	22.260440698843745	27.429581520317125	27.86658738703163	22.4433903938075
52-53	22.437511945303953	27.386037399924145	27.823875270257005	22.3525753845149
54-55	22.382315744371336	27.35860469002626	27.75466339945638	22.504416166146022
56-57	22.43136517797816	27.509608298008104	27.62496832957685	22.434058194436886
58-59	22.290168926175344	27.61982533500289	27.773314988609915	22.316690750211855
60-61	22.449691409642185	27.70463730683478	27.6076187073458	22.238052576177235
62-63	22.316976146163352	27.84070651574594	27.560633603652914	22.28168373443779
64-65	22.332482439291766	27.78377191171585	27.495342603426394	22.388403045565997
66-67	22.365855124723193	27.767856122713148	27.396006972264868	22.470281780298787
68-69	22.338375104529476	27.659409624482574	27.502954661173725	22.499260609814232
70-71	22.404865191931002	27.36654638472932	27.61633431639277	22.612254106946907
72-73	22.47286093293712	27.393149149409258	27.49284730379532	22.641142613858296
74-75	22.369210902955633	27.525715126210542	27.458296342742912	22.646777628090913
76-77	22.522533542047036	27.437785828722006	27.442485683068696	22.597194946162265
78-79	22.437336971036483	27.457486585736373	27.39391034813406	22.711266095093087
80-81	22.464969075496416	27.51316157398333	27.342030985740223	22.67983836478003
82-83	22.50036155787461	27.521499647636382	27.43660809511929	22.541530699369723
84-85	22.588374062109292	27.485630426423135	27.40184465515502	22.52415085631255
86-87	22.41584189312777	27.53718751203124	27.49990533574668	22.547065259094317
88-89	22.4874871706256	27.53802565316838	27.442427130019315	22.53206004618671
90-91	22.532278636546756	27.56163967325479	27.430294928359327	22.475786761839124
92-93	22.556256264947077	27.546745384657882	27.47624910876606	22.42074924162898
94-95	22.452366850495935	27.590971056819484	27.51236540467526	22.44429668800932
96-97	22.4757956696783	27.717695184457057	27.576986953482123	22.229522192382525
98-99	23.54675247047988	29.636471587509526	27.75542388023291	19.06135206177768
100	22.638039692074337	30.526086759043537	21.43504996432736	25.40082358455477
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2282.0
1	2052.5
2	2872.5
3	3991.0
4	5004.5
5	8443.5
6	11542.0
7	13585.0
8	14838.0
9	14211.5
10	12100.0
11	9580.5
12	9674.0
13	13305.5
14	23897.0
15	39539.5
16	53204.0
17	62476.5
18	65251.0
19	61294.0
20	53434.0
21	44502.5
22	38337.5
23	37924.5
24	42968.5
25	53437.5
26	68643.0
27	86660.0
28	106083.0
29	128083.0
30	150977.5
31	173758.5
32	199404.0
33	224904.5
34	248843.5
35	272076.0
36	297133.5
37	319843.0
38	337402.0
39	352603.0
40	367928.0
41	380593.0
42	387884.5
43	396360.0
44	402949.5
45	412231.5
46	422322.5
47	425068.5
48	424556.0
49	417525.0
50	406054.5
51	392949.5
52	376590.5
53	358531.5
54	339612.5
55	317962.5
56	292755.5
57	265790.5
58	238430.0
59	209147.0
60	175973.5
61	143740.0
62	114735.0
63	88904.0
64	67037.0
65	49639.5
66	36777.5
67	27911.0
68	21460.5
69	16042.0
70	11926.5
71	8715.0
72	6099.5
73	4163.0
74	2827.5
75	1887.0
76	1281.0
77	896.0
78	658.5
79	484.5
80	341.5
81	234.0
82	164.0
83	113.5
84	76.5
85	54.5
86	39.0
87	27.0
88	18.0
89	13.0
90	9.5
91	6.0
92	4.0
93	3.0
94	2.5
95	2.5
96	2.0
97	2.0
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0051329442608459815
3	3.0134702118469562E-5
4	2.008980141231304E-5
5	0.004680923729068938
6	0.005052585055196729
7	7.031430494309564E-5
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.01087301300586203
26-27	0.0021712759789778266
28-29	0.0
30-31	3.7759842467951084E-4
32-33	0.0027095275920983315
34-35	5.36371747721293E-4
36-37	0.0016409483990821052
38-39	3.0655303086427006E-5
40-41	1.0285984836504013E-5
42-43	1.0360309818848947E-5
44-45	0.0
46-47	0.002006607017632795
48-49	0.0
50-51	6.882612209431437E-4
52-53	5.4158360128182004E-6
54-55	1.3089767663168863E-4
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0010768016060693274
64-65	5.97135014444696E-4
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0028989780688167695
74-75	3.2831777196635424E-4
76-77	0.003277739694807488
78-79	0.0
80-81	0.0
82-83	8.635070550079465E-4
84-85	4.5187627502068747E-4
86-87	0.0017251747199239099
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0015085435966095684
96-97	8.858924825003864E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	316.0
20-21	1102.0
22-23	2106.0
24-25	2766.0
26-27	4593.0
28-29	8453.0
30-31	20491.0
32-33	26320.0
34-35	35455.0
36-37	52254.0
38-39	62842.0
40-41	67982.0
42-43	79446.0
44-45	97774.0
46-47	94946.0
48-49	81582.0
50-51	69009.0
52-53	63615.0
54-55	68227.0
56-57	74131.0
58-59	77175.0
60-61	76883.0
62-63	76022.0
64-65	82288.0
66-67	85576.0
68-69	89255.0
70-71	84435.0
72-73	75461.0
74-75	76683.0
76-77	80456.0
78-79	83926.0
80-81	84536.0
82-83	81933.0
84-85	82717.0
86-87	86797.0
88-89	92506.0
90-91	97445.0
92-93	106931.0
94-95	130387.0
96-97	343119.0
98-99	640488.0
100-101	6406871.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.11950393749098
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.79805837259244	76.43566183224928
2	7.186575646674085	12.234355081083033
3	1.6931860375924492	4.323694667812657
4	0.6087700930012618	2.0727283331299065
5	0.27836749792525434	1.18472516678591
6	0.1467596816198899	0.7495266778505484
7	0.07936807760928233	0.47290399762020785
8	0.049776845490680816	0.33895843165919093
9	0.03630158511765376	0.2780975625623364
>10	0.1187648916295451	1.5934406269995058
>50	0.0030808430373540268	0.17865280121323066
>100	9.90427710109283E-4	0.13725482103417272
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.013470211846956E-5	0.0	1.0044900706156519E-5	0.0
3	0.0	3.013470211846956E-5	0.0	3.013470211846956E-5	0.0
4	0.0	9.040410635540868E-5	0.0	5.02245035307826E-5	0.0
5	0.0	1.004490070615652E-4	0.0	5.02245035307826E-5	0.0
6	0.0	1.004490070615652E-4	0.0	6.026940423693912E-5	3.013470211846956E-5
7	0.0	1.004490070615652E-4	0.0	6.026940423693912E-5	3.013470211846956E-5
8	0.0	1.2053880847387823E-4	0.0	8.035920564925215E-5	4.0179602824626076E-5
9	0.0	2.2098781553544342E-4	0.0	8.035920564925215E-5	6.026940423693912E-5
10-11	0.0	2.2098781553544342E-4	0.0	1.4062860988619126E-4	6.026940423693912E-5
12-13	0.0	2.2098781553544342E-4	0.0	1.7076331200466084E-4	7.031430494309564E-5
14-15	5.0224503530782595E-6	3.616164254216347E-4	0.0	1.7578576235773908E-4	8.035920564925215E-5
16-17	1.506735105923478E-5	3.7166132612779124E-4	0.0	1.9085311341697386E-4	9.040410635540868E-5
18-19	2.0089801412313038E-5	3.9677357789318253E-4	0.0	1.9587556377005213E-4	1.1049390776772171E-4
20-21	2.0089801412313038E-5	4.218858296585738E-4	0.0	2.109429148292869E-4	1.4062860988619126E-4
22-23	2.0089801412313038E-5	4.4197563107088684E-4	0.0	2.4107761694775647E-4	1.6574086165158257E-4
24-25	4.0179602824626076E-5	4.721103331893564E-4	0.0	2.561449680069912E-4	2.109429148292869E-4
26-27	4.0179602824626076E-5	4.8215523389551294E-4	0.0	3.7166132612779124E-4	2.4107761694775647E-4
28-29	4.0179602824626076E-5	4.871776842485912E-4	0.0	6.529185459001738E-4	2.4107761694775647E-4
30-31	5.02245035307826E-5	5.876266913101564E-4	0.0	0.0018482617299327994	2.51122517653913E-4
32-33	6.026940423693912E-5	6.026940423693912E-4	0.0	0.004711058431187408	2.7121231906622603E-4
34-35	6.529185459001739E-5	6.830532480186433E-4	0.0	0.008422649242112243	3.113919218908521E-4
36-37	7.031430494309564E-5	7.634124536678954E-4	0.0	0.015122598013118639	3.113919218908521E-4
38-39	7.031430494309564E-5	7.835022550802085E-4	0.0	0.03438369511717377	3.1641437224393034E-4
40-41	7.031430494309564E-5	8.437716593171476E-4	0.0	0.055483009050455535	3.214368225970086E-4
42-43	7.031430494309564E-5	8.88973712494852E-4	0.0	0.06791357367432423	3.365041736562434E-4
44-45	7.031430494309564E-5	9.84400269203339E-4	0.0	0.08264944301025584	3.616164254216347E-4
46-47	7.031430494309564E-5	0.0010346247727341215	0.0	0.09794280433537914	3.7668377648086946E-4
48-49	7.53367552961739E-5	0.0010446696734402782	0.0	0.11215633883459061	4.3193073036473036E-4
50-51	8.538165600233041E-5	0.001215432985444939	0.0	0.12600323445802739	4.3695318071780857E-4
52-53	9.040410635540868E-5	0.0012405452372103302	0.0	0.1689401625264934	4.8215523389551294E-4
54-55	9.040410635540868E-5	0.0012606350386226432	0.0	0.2213594768615712	5.323797374262955E-4
56-57	9.040410635540868E-5	0.001295792191094191	0.0	0.2693640573362932	5.675368898978433E-4
58-59	9.040410635540868E-5	0.0013058370918003475	0.0	0.29110122246441594	5.976715920163128E-4
60-61	1.004490070615652E-4	0.0013108595421534258	0.0	0.3045362771589003	6.227838437817042E-4
62-63	1.004490070615652E-4	0.00135606159533113	0.0	0.31628378853475037	6.579409962532521E-4
64-65	1.004490070615652E-4	0.0015167800066296345	0.0	0.32254176167468585	6.830532480186434E-4
66-67	1.004490070615652E-4	0.0015368698080419475	0.0	0.32782035699577106	7.081654997840347E-4
68-69	1.1049390776772171E-4	0.0015820718612196518	0.0	0.3334354564905126	7.182104004901911E-4
70-71	1.1049390776772171E-4	0.0016071841129850432	0.0	0.33816660472311233	7.583900033148172E-4
72-73	1.1049390776772171E-4	0.0016071841129850432	0.0	0.3415768485128524	8.437716593171476E-4
74-75	1.1049390776772171E-4	0.0016373188151035127	0.0	0.3446003636254056	8.48794109670226E-4
76-77	1.2053880847387823E-4	0.0016925657689873734	0.0	0.3475485419826625	8.538165600233042E-4
78-79	1.3058370918003474E-4	0.0017176780207527648	0.0	0.34941187106365457	8.538165600233042E-4
80-81	1.3058370918003474E-4	0.0017176780207527648	0.0	0.3500396773577893	8.638614607294607E-4
82-83	1.3058370918003474E-4	0.001722700471105843	0.0	0.35043142848532943	8.839512621417737E-4
84-85	1.3058370918003474E-4	0.0017277229214589214	0.0	0.3505419223930971	8.839512621417737E-4
86-87	1.3058370918003474E-4	0.0017478127228712345	0.0	0.35058210199592177	8.889737124948519E-4
88	1.3058370918003474E-4	0.0018683515313451127	0.0	0.3505871244462748	8.939961628479302E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14220	0.0	51.74651	1
GTATCAA	26650	0.0	39.78165	1
TCAACGC	32390	0.0	32.047832	4
ATCAACG	32695	0.0	31.761871	3
TATCAAC	33530	0.0	31.251219	2
CAACGCA	33385	0.0	31.054487	5
AACGCAG	34330	0.0	30.223354	6
ACGCAGA	39865	0.0	26.015064	7
CGCAGAG	40480	0.0	25.598827	8
GTGGTAT	6405	0.0	24.755589	1
TGGTATC	6360	0.0	23.727655	2
GCAGAGT	47005	0.0	22.099579	9
GTACATG	33090	0.0	21.402367	1
TACATGG	33470	0.0	20.664047	2
CTTATAC	9810	0.0	20.409645	1
GAGTACT	28095	0.0	20.370619	12-13
ACATGGG	34025	0.0	19.66394	3
CAGAGTA	45715	0.0	19.213371	10-11
AGAGTAC	43060	0.0	18.601543	10-11
GTACTTT	31025	0.0	18.249588	14-15
>>END_MODULE