FastQCFastQC Report
Fri 27 May 2016
SRR2140369_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140369_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9842733
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA137200.052.9635161
GTATCAA266250.040.3368761
TCAACGC328100.032.249444
ATCAACG332100.031.8995953
TATCAAC337600.031.558812
CAACGCA338400.031.2552245
AACGCAG349000.030.3166266
ACGCAGA405850.026.1107547
CGCAGAG413200.025.6049488
GTGGTAT62200.023.9696481
TGGTATC59100.023.564422
GCAGAGT476200.022.2085139
GTACATG352700.021.668631
TACATGG354600.021.1911092
GAGTACT270000.020.6049812-13
ACATGGG359750.020.2691293
CAGAGTA461500.019.20457310-11
AGAGTAC435350.018.87664210-11
GTACTTT297200.018.65518414-15
CATGGGG260000.018.6148244
Produced by FastQC (version 0.11.5)