##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140369_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9842733
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.401416151388034	34.0	31.0	34.0	31.0	34.0
2	32.486168729762355	34.0	31.0	34.0	31.0	34.0
3	32.53881579435305	34.0	31.0	34.0	31.0	34.0
4	36.05998374638426	37.0	35.0	37.0	35.0	37.0
5	36.10437903781399	37.0	35.0	37.0	35.0	37.0
6	36.093313412037084	37.0	36.0	37.0	35.0	37.0
7	36.06068487278889	37.0	35.0	37.0	35.0	37.0
8	36.046326360778046	37.0	35.0	37.0	35.0	37.0
9	37.7875916170844	39.0	38.0	39.0	35.0	39.0
10-11	37.727978956657665	39.0	37.5	39.0	35.0	39.0
12-13	37.714696720920905	39.0	37.0	39.0	35.0	39.0
14-15	39.06109492150198	40.0	38.0	41.0	36.0	41.0
16-17	39.07461083217436	40.0	38.0	41.0	36.0	41.0
18-19	39.11838312590619	40.0	38.5	41.0	36.0	41.0
20-21	39.14311147695027	40.0	39.0	41.0	36.0	41.0
22-23	39.10466167705915	40.0	39.0	41.0	36.0	41.0
24-25	39.07125993219742	40.0	39.0	41.0	36.0	41.0
26-27	39.012803963241616	40.0	39.0	41.0	35.0	41.0
28-29	38.97906879545156	40.0	38.0	41.0	35.0	41.0
30-31	38.903605155496024	40.0	38.0	41.0	35.0	41.0
32-33	38.80395247401397	40.0	38.0	41.0	35.0	41.0
34-35	38.73994821946405	40.0	38.0	41.0	35.0	41.0
36-37	38.69333958614981	40.0	38.0	41.0	35.0	41.0
38-39	38.65400178136831	40.0	38.0	41.0	35.0	41.0
40-41	38.595694423392615	40.0	38.0	41.0	35.0	41.0
42-43	38.527981440403785	40.0	38.0	41.0	34.0	41.0
44-45	38.482214679126294	40.0	38.0	41.0	34.0	41.0
46-47	38.40307281257032	40.0	38.0	41.0	34.0	41.0
48-49	38.32855513866075	40.0	38.0	41.0	34.0	41.0
50-51	38.25136445492762	40.0	37.5	41.0	34.0	41.0
52-53	38.141365291312894	40.0	37.0	41.0	33.5	41.0
54-55	38.02361045677546	40.0	37.0	41.0	33.0	41.0
56-57	37.87961096775572	40.0	37.0	41.0	33.0	41.0
58-59	37.72730484633682	39.0	36.0	41.0	33.0	41.0
60-61	37.5406951407694	39.0	36.0	41.0	33.0	41.0
62-63	37.31982710767409	39.0	35.0	41.0	32.5	41.0
64-65	37.08168972845928	38.5	35.0	40.0	32.0	41.0
66-67	36.796096504510885	38.0	35.0	40.0	32.0	41.0
68-69	36.516849870981844	37.0	35.0	40.0	32.0	41.0
70-71	36.204193224758185	37.0	35.0	39.0	31.0	41.0
72-73	35.876052074352245	36.0	35.0	39.0	31.0	41.0
74-75	35.556631348556344	36.0	34.0	39.0	31.0	40.0
76-77	35.235902793054066	35.0	34.0	37.5	31.0	39.5
78-79	34.947939419886545	35.0	34.0	37.0	31.0	39.0
80-81	34.669773599916695	35.0	34.0	37.0	31.0	39.0
82-83	34.400742531235124	35.0	34.0	36.0	31.0	38.5
84-85	34.17929578046673	35.0	34.0	36.0	30.0	37.0
86-87	33.990370964365866	35.0	34.0	35.5	30.0	37.0
88-89	33.8544025488229	35.0	34.0	35.0	30.0	36.5
90-91	33.76248108778175	35.0	34.0	35.0	30.0	36.0
92-93	33.68351953878866	35.0	34.0	35.0	30.0	36.0
94-95	33.59760073938125	35.0	34.0	35.0	30.0	36.0
96-97	33.60795913042222	35.0	34.0	35.0	30.0	35.5
98-99	33.69240536704968	35.0	34.0	35.0	31.0	35.0
100	32.90656354693472	34.0	32.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	11.0
19	17.0
20	85.0
21	306.0
22	1084.0
23	3223.0
24	8189.0
25	17307.0
26	31705.0
27	51886.0
28	77495.0
29	106664.0
30	141937.0
31	183322.0
32	235038.0
33	309749.0
34	436310.0
35	647373.0
36	1094375.0
37	2168193.0
38	2865761.0
39	1419066.0
40	43635.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.480989070820065	19.96406892272705	14.338862996690047	24.216079009762836
2	14.512214030066023	20.83141726560082	41.613810968801104	23.042557735532053
3	18.120409683085892	26.12714155523925	30.155241482799994	25.59720727887487
4	12.6204848899614	18.141281563403965	38.305081680607245	30.933151866027387
5	13.595737717809941	37.59449450475649	34.86198618017709	13.947781597256478
6	29.929250365595152	37.49359770318967	18.68838694227117	13.888764988944006
7	26.691736962247443	32.81015499367858	22.55435588765874	17.943752156415236
8	24.706542380048305	36.50120347671729	20.761835152899096	18.030418990335306
9	25.823213938648948	17.193476649219278	20.964654837228643	36.01865457490313
10-11	24.46188980235469	26.82717290004717	28.239199417478865	20.471737880119274
12-13	25.368137081438665	24.291144542882552	28.508408182971134	21.832310192707656
14-15	22.523942283103686	25.516195552597026	26.385918423267196	25.57394374103209
16-17	21.90532853019583	28.68562014229178	26.986645883821087	22.4224054436913
18-19	21.949406734897718	27.609323548652593	28.963556158640085	21.477713557809604
20-21	22.849776090813993	26.658983142255323	28.924638992126972	21.56660177480371
22-23	22.57793335683684	26.611101652465237	28.77334809999853	22.037616890699393
24-25	22.28994272297129	26.802462861411954	28.85688874432693	22.050705671289826
26-27	22.14585104855832	26.92008639133191	28.88968899689839	22.04437356321138
28-29	22.02867525769578	26.836445124090364	28.919946501278627	22.214933116935228
30-31	22.363186561973993	26.69663631855437	28.835465440115517	22.10471167935612
32-33	22.126249633865562	26.913724313127712	28.81097034439877	22.149055708607957
34-35	22.3204018482778	27.000767063322062	28.768895597637684	21.909935490762447
36-37	22.29845822517607	26.85487601445022	28.61924042823934	22.22742533213437
38-39	22.174003000933098	26.985461548498623	28.733384510676423	22.107150939891852
40-41	22.44802946190379	26.8344746336641	28.576730150133763	22.140765754298346
42-43	22.375317589320147	26.897881559332838	28.41023905143867	22.316561799908346
44-45	22.37826165394438	27.093286557891123	28.218392601838442	22.31005918632605
46-47	22.62743419006599	27.02464863736479	27.783790030205047	22.564127142364175
48-49	22.46505289730621	27.196038338555123	27.73179134955917	22.6071174145795
50-51	22.429740219214416	27.323979448106684	27.69426674987973	22.552013582799166
52-53	22.63298865153833	27.245347237636892	27.608111129834494	22.513552980990287
54-55	22.57960328524991	27.142503479604617	27.641790146238698	22.636103088906776
56-57	22.580123723005944	27.34662928243955	27.527333978736234	22.545913015818275
58-59	22.48042702065882	27.40488728117899	27.641202565367777	22.47348313279441
60-61	22.63868777503224	27.43218933717403	27.510891075635595	22.418231812158137
62-63	22.508814810809326	27.580464091584478	27.46367769675736	22.447043400848838
64-65	22.501110201212114	27.496507123000697	27.44338545203302	22.55899722375417
66-67	22.569146398793897	27.534305462659457	27.29214925565152	22.604398882895122
68-69	22.511522030821016	27.446904125941433	27.421965055632974	22.619608787604577
70-71	22.565869745926847	27.135751244325267	27.51459851077479	22.783780498973094
72-73	22.585995802515267	27.23943302659893	27.39705801462576	22.777513156260046
74-75	22.4452529001832	27.389420278974214	27.38955178486974	22.77577503597284
76-77	22.595172657595292	27.277907549369722	27.354731232894984	22.77218856014
78-79	22.58623387907386	27.31433683873623	27.29165640741359	22.807772874776322
80-81	22.571917580432206	27.36189443092653	27.242544584221772	22.823643404419492
82-83	22.62309589369208	27.333224602794658	27.355916463407404	22.687763040105864
84-85	22.67750877160208	27.318156283810925	27.319723508896605	22.684611435690393
86-87	22.494516794059397	27.399415462387488	27.404683392344282	22.70138435120884
88-89	22.56518438893489	27.335849265254318	27.37252527339177	22.72644107241902
90-91	22.618935246671423	27.31830455000468	27.40725260379744	22.655507599526455
92-93	22.62006543690046	27.332086042116398	27.414953990293945	22.6328945306892
94-95	22.519302935977194	27.423939563634825	27.407835221326987	22.64892227906099
96-97	22.544584653930507	27.554750120539396	27.47457819350718	22.426087032022917
98-99	23.64373658951316	29.448292875272465	27.627188447444517	19.280782087769857
100	22.73477216543212	30.478669011764236	21.2737058185496	25.512853004254044
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2401.0
1	2015.0
2	2542.0
3	3590.5
4	4470.0
5	7272.0
6	9907.0
7	12130.0
8	14074.5
9	14020.0
10	12054.5
11	9605.0
12	9570.0
13	12789.5
14	22261.5
15	36308.5
16	48786.0
17	56989.5
18	59137.0
19	55679.5
20	48877.0
21	41137.5
22	36272.0
23	36849.0
24	42557.0
25	53498.0
26	68968.5
27	86556.5
28	104643.5
29	124325.5
30	143757.5
31	164020.0
32	188131.0
33	212642.5
34	236503.0
35	258248.0
36	282732.0
37	305317.5
38	320470.5
39	333775.5
40	348537.5
41	362502.0
42	371963.5
43	381174.0
44	393446.5
45	406240.0
46	409173.5
47	407375.5
48	407526.5
49	404890.5
50	399692.5
51	390276.5
52	377213.0
53	361052.0
54	343561.0
55	324548.0
56	301978.5
57	276857.0
58	250305.5
59	220939.5
60	187586.0
61	154301.0
62	122915.0
63	95556.0
64	72854.5
65	54418.0
66	40480.0
67	30900.5
68	24049.5
69	18104.5
70	13401.5
71	9795.0
72	6906.5
73	4845.0
74	3392.0
75	2289.0
76	1582.5
77	1077.5
78	750.0
79	555.5
80	385.5
81	254.0
82	183.5
83	134.0
84	97.0
85	73.5
86	53.0
87	37.5
88	24.0
89	14.5
90	10.5
91	6.5
92	3.5
93	1.0
94	0.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.005354203959408429
3	3.0479339427372457E-5
4	5.0798899045620764E-5
5	0.005018931225707332
6	0.005709796252727773
7	9.143801828211737E-5
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.01084944000882184
26-27	0.002160346619227827
28-29	0.0
30-31	3.869445716157013E-4
32-33	0.0027907326045524756
34-35	5.525035419058077E-4
36-37	0.003071098655757522
38-39	3.0981095387229874E-5
40-41	2.597720936295518E-5
42-43	0.0
44-45	0.0
46-47	0.002944798657512621
48-49	0.0
50-51	0.0014247867275753061
52-53	5.455177858436826E-6
54-55	4.0097891586946016E-4
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0016323982072186688
64-65	0.0010056276293448543
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0027798316986757275
74-75	3.2278615620387225E-4
76-77	0.0031538989940630116
78-79	0.0
80-81	0.0
82-83	9.250495087738774E-4
84-85	6.644990207102403E-4
86-87	0.0016588638859127128
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0016223764543707288
96-97	0.0010785344710809296
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	272.0
20-21	948.0
22-23	1794.0
24-25	2473.0
26-27	4347.0
28-29	7916.0
30-31	19178.0
32-33	24551.0
34-35	33807.0
36-37	49754.0
38-39	58830.0
40-41	61833.0
42-43	73953.0
44-45	90512.0
46-47	89493.0
48-49	76521.0
50-51	65779.0
52-53	61808.0
54-55	66520.0
56-57	69773.0
58-59	72696.0
60-61	70989.0
62-63	70147.0
64-65	74672.0
66-67	78793.0
68-69	82593.0
70-71	78950.0
72-73	71627.0
74-75	72191.0
76-77	75898.0
78-79	79888.0
80-81	81069.0
82-83	77759.0
84-85	78199.0
86-87	82636.0
88-89	88338.0
90-91	94113.0
92-93	102916.0
94-95	125963.0
96-97	339317.0
98-99	640404.0
100-101	6443513.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.38424760117212
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.32917317041968	77.12688487592268
2	6.839808931656447	11.680238787305251
3	1.5533090968165681	3.9788438557121673
4	0.5495823560784093	1.8770270387453776
5	0.2559936408477032	1.0928912207232913
6	0.15233922561700414	0.7804422095671862
7	0.08576420767533545	0.5126038640428432
8	0.05564930302264746	0.38012590944947094
9	0.03593206276848303	0.27612289298205117
>10	0.1374608375436412	1.8443260647013824
>50	0.003927948968644022	0.2274749355917427
>100	9.584476299191984E-4	0.13050582081238218
>500	6.046297224072436E-5	0.03343631338789738
>1k	4.0307983287386896E-5	0.05907621105627714
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0159779809124153E-5
2	0.0	2.0319559618248307E-5	0.0	0.0	1.0159779809124153E-5
3	0.0	2.0319559618248307E-5	0.0	1.0159779809124153E-5	1.0159779809124153E-5
4	0.0	4.0639119236496614E-5	0.0	1.0159779809124153E-5	1.0159779809124153E-5
5	0.0	5.0798899045620764E-5	0.0	1.0159779809124153E-5	1.0159779809124153E-5
6	1.0159779809124153E-5	5.0798899045620764E-5	0.0	1.0159779809124153E-5	2.0319559618248307E-5
7	1.0159779809124153E-5	5.0798899045620764E-5	0.0	1.0159779809124153E-5	2.0319559618248307E-5
8	1.0159779809124153E-5	5.0798899045620764E-5	0.0	1.0159779809124153E-5	3.0479339427372457E-5
9	1.0159779809124153E-5	1.2191735770948983E-4	0.0	2.0319559618248307E-5	3.0479339427372457E-5
10-11	1.0159779809124153E-5	1.2191735770948983E-4	0.0	2.5399449522810382E-5	3.0479339427372457E-5
12-13	1.0159779809124153E-5	1.2191735770948983E-4	0.0	4.0639119236496614E-5	3.0479339427372457E-5
14-15	1.523966971368623E-5	1.930358163733589E-4	0.0	4.0639119236496614E-5	3.5559229331934535E-5
16-17	2.0319559618248307E-5	1.930358163733589E-4	0.0	6.0958678854744914E-5	6.0958678854744914E-5
18-19	2.0319559618248307E-5	2.0827548608704513E-4	0.0	8.127823847299323E-5	7.111845866386907E-5
20-21	2.0319559618248307E-5	2.3367493560985553E-4	0.0	9.651790818667945E-5	7.111845866386907E-5
22-23	2.0319559618248307E-5	2.997135043691625E-4	0.0	1.269972476140519E-4	7.619834856843115E-5
24-25	3.5559229331934535E-5	3.5559229331934533E-4	0.0	1.7271625675511059E-4	9.143801828211737E-5
26-27	5.0798899045620764E-5	3.860716327467178E-4	0.0	2.2351515580073134E-4	1.0159779809124153E-4
28-29	5.0798899045620764E-5	3.860716327467178E-4	0.0	5.33388439979018E-4	1.066776879958036E-4
30-31	5.0798899045620764E-5	4.724297611242731E-4	0.0	0.0016052452098416162	1.2191735770948983E-4
32-33	5.5878788950182836E-5	4.927493207425214E-4	0.0	0.004267107519832144	1.4223691732773814E-4
34-35	6.0958678854744914E-5	5.587878895018284E-4	0.0	0.0076655538659841736	1.4223691732773814E-4
36-37	6.0958678854744914E-5	6.248264582611354E-4	0.0	0.013446468577375817	1.4223691732773814E-4
38-39	6.0958678854744914E-5	6.502259077839458E-4	0.0	0.027512683723108205	1.5747658704142438E-4
40-41	8.127823847299323E-5	6.908650270204423E-4	0.0	0.04246787960213896	1.6255647694598646E-4
42-43	8.127823847299323E-5	7.213443664478149E-4	0.0	0.05168787977891913	1.6255647694598646E-4
44-45	8.127823847299323E-5	8.381818342527426E-4	0.0	0.0628890370184785	1.6255647694598646E-4
46-47	9.143801828211737E-5	9.245399626302979E-4	0.0	0.07479629895477202	1.6255647694598646E-4
48-49	9.143801828211737E-5	9.34699742439422E-4	0.0	0.08548946720387518	1.6255647694598646E-4
50-51	1.0159779809124153E-4	0.001061696990053474	0.0	0.0962029550125966	1.6255647694598646E-4
52-53	1.0159779809124153E-4	0.001066776879958036	0.0	0.12084042104972267	1.7779614665967266E-4
54-55	1.0159779809124153E-4	0.0010769366597671603	0.0	0.14710853174621316	2.0827548608704513E-4
56-57	1.0159779809124153E-4	0.0011074159991945327	0.0	0.1759877058536486	2.2351515580073137E-4
58-59	1.0159779809124153E-4	0.0011175757790036567	0.0	0.19589579438962734	2.3367493560985553E-4
60-61	1.0159779809124153E-4	0.0011632947881447156	0.0	0.20460780557595132	2.3367493560985553E-4
62-63	1.0159779809124153E-4	0.0012140936871903362	0.0	0.2144932713302291	2.3367493560985553E-4
64-65	1.0159779809124153E-4	0.0013106115953770156	0.0	0.219517282445841	2.3367493560985553E-4
66-67	1.0159779809124153E-4	0.0013106115953770156	0.0	0.22422634038737005	2.387548255144176E-4
68-69	1.0159779809124153E-4	0.0013207713751861398	0.0	0.229296070512123	2.4383471541897966E-4
70-71	1.0159779809124153E-4	0.0013461708247089503	0.0	0.2339035306555608	2.539944952281038E-4
72-73	1.0159779809124153E-4	0.0013664903843271987	0.0	0.23747977314837249	3.1495317408284875E-4
74-75	1.0159779809124153E-4	0.001396969723754571	0.0	0.24024831314635886	3.1495317408284875E-4
76-77	1.0159779809124153E-4	0.0014426887328956297	0.0	0.2431895694011003	3.1495317408284875E-4
78-79	1.0159779809124153E-4	0.0014731680723230021	0.0	0.24496245097779246	3.1495317408284875E-4
80-81	1.0159779809124153E-4	0.0014731680723230021	0.0	0.24548567963796236	3.1495317408284875E-4
82-83	1.0159779809124153E-4	0.0014782479622275644	0.0	0.24587683116061362	3.4543251351022117E-4
84-85	1.0159779809124153E-4	0.0014833278521321264	0.0	0.24595302950918205	3.4543251351022117E-4
86-87	1.0159779809124153E-4	0.001534126751177747	0.0	0.24598350884860942	3.4543251351022117E-4
88	1.0159779809124153E-4	0.0016255647694598644	0.0	0.24598858873851398	3.4543251351022117E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13720	0.0	52.963516	1
GTATCAA	26625	0.0	40.336876	1
TCAACGC	32810	0.0	32.24944	4
ATCAACG	33210	0.0	31.899595	3
TATCAAC	33760	0.0	31.55881	2
CAACGCA	33840	0.0	31.255224	5
AACGCAG	34900	0.0	30.316626	6
ACGCAGA	40585	0.0	26.110754	7
CGCAGAG	41320	0.0	25.604948	8
GTGGTAT	6220	0.0	23.969648	1
TGGTATC	5910	0.0	23.56442	2
GCAGAGT	47620	0.0	22.208513	9
GTACATG	35270	0.0	21.66863	1
TACATGG	35460	0.0	21.191109	2
GAGTACT	27000	0.0	20.60498	12-13
ACATGGG	35975	0.0	20.269129	3
CAGAGTA	46150	0.0	19.204573	10-11
AGAGTAC	43535	0.0	18.876642	10-11
GTACTTT	29720	0.0	18.655184	14-15
CATGGGG	26000	0.0	18.614824	4
>>END_MODULE