FastQCFastQC Report
Fri 27 May 2016
SRR2140368_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140368_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9800409
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA150050.054.836691
GTATCAA282100.039.446311
TCAACGC343000.031.8921814
ATCAACG346600.031.5856043
TATCAAC354000.031.0962072
CAACGCA352450.031.0249425
AACGCAG362000.030.2178176
ACGCAGA411400.026.5674537
CGCAGAG419000.026.1059748
TGGTATC59500.022.7128832
GCAGAGT483700.022.6051839
GTGGTAT63450.022.3773481
GAGTACT302400.021.09088912-13
CAGAGTA472150.020.16490210-11
GTACATG310650.019.8103241
TACATGG306550.019.4614412
AGAGTAC441550.019.3833610-11
GTACTTT330300.019.19981614-15
ACATGGG311700.018.6449153
AGTACTT322750.017.70082912-13
Produced by FastQC (version 0.11.5)