##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140368_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9800409
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.42822529141386	34.0	31.0	34.0	31.0	34.0
2	32.51662823459715	34.0	31.0	34.0	31.0	34.0
3	32.5654595639835	34.0	31.0	34.0	31.0	34.0
4	36.08291153971227	37.0	35.0	37.0	35.0	37.0
5	36.129239300114925	37.0	35.0	37.0	35.0	37.0
6	36.11664247890062	37.0	36.0	37.0	35.0	37.0
7	36.08315601930491	37.0	36.0	37.0	35.0	37.0
8	36.06824174378845	37.0	35.0	37.0	35.0	37.0
9	37.812856075700516	39.0	38.0	39.0	35.0	39.0
10-11	37.75501593862052	39.0	37.5	39.0	35.0	39.0
12-13	37.74352504063861	39.0	37.5	39.0	35.0	39.0
14-15	39.09755174503431	40.0	38.0	41.0	36.0	41.0
16-17	39.114561137193355	40.0	38.5	41.0	36.0	41.0
18-19	39.161416732709824	40.0	38.5	41.0	36.0	41.0
20-21	39.18790018050157	40.0	39.0	41.0	36.0	41.0
22-23	39.14919307275031	40.0	39.0	41.0	36.0	41.0
24-25	39.11759881400543	40.0	39.0	41.0	36.0	41.0
26-27	39.06012065508274	40.0	39.0	41.0	36.0	41.0
28-29	39.02547709521444	40.0	39.0	41.0	35.0	41.0
30-31	38.94326645881007	40.0	38.0	41.0	35.0	41.0
32-33	38.84111353714816	40.0	38.0	41.0	35.0	41.0
34-35	38.78005237403708	40.0	38.0	41.0	35.0	41.0
36-37	38.733222314301045	40.0	38.0	41.0	35.0	41.0
38-39	38.69997327206458	40.0	38.0	41.0	35.0	41.0
40-41	38.63869380212537	40.0	38.0	41.0	35.0	41.0
42-43	38.57511548948723	40.0	38.0	41.0	35.0	41.0
44-45	38.53104914669066	40.0	38.0	41.0	34.0	41.0
46-47	38.457829351373896	40.0	38.0	41.0	34.0	41.0
48-49	38.39440582498463	40.0	38.0	41.0	34.0	41.0
50-51	38.326948943535356	40.0	38.0	41.0	34.0	41.0
52-53	38.2183814470324	40.0	37.5	41.0	34.0	41.0
54-55	38.11338652583454	40.0	37.0	41.0	33.5	41.0
56-57	37.98226560115802	40.0	37.0	41.0	33.0	41.0
58-59	37.83822371242667	39.5	36.5	41.0	33.0	41.0
60-61	37.66569081336634	39.0	36.0	41.0	33.0	41.0
62-63	37.455235979114875	39.0	35.5	41.0	33.0	41.0
64-65	37.22377299749894	39.0	35.0	40.5	32.5	41.0
66-67	36.94881792728686	38.0	35.0	40.0	32.0	41.0
68-69	36.67522271687485	37.5	35.0	40.0	32.0	41.0
70-71	36.36473568206653	37.0	35.0	39.5	32.0	41.0
72-73	36.0373159753865	36.5	35.0	39.0	31.0	41.0
74-75	35.717855917755806	36.0	35.0	39.0	31.0	40.5
76-77	35.38992929471387	35.5	34.0	38.5	31.0	40.0
78-79	35.09380736414061	35.0	34.0	37.0	31.0	39.0
80-81	34.80660016319807	35.0	34.0	37.0	31.0	39.0
82-83	34.52719654818811	35.0	34.0	36.5	31.0	38.5
84-85	34.29418992866732	35.0	34.0	36.0	31.0	37.0
86-87	34.09195348171795	35.0	34.0	36.0	31.0	37.0
88-89	33.94627279974715	35.0	34.0	35.0	31.0	36.5
90-91	33.842785981112485	35.0	34.0	35.0	31.0	36.0
92-93	33.75761371822823	35.0	34.0	35.0	31.0	36.0
94-95	33.666404728633836	35.0	34.0	35.0	30.0	36.0
96-97	33.67045322807425	35.0	34.0	35.0	30.5	36.0
98-99	33.74857121827232	35.0	34.0	35.0	31.0	35.0
100	32.97184493244513	34.0	32.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	6.0
19	26.0
20	90.0
21	297.0
22	933.0
23	3063.0
24	7359.0
25	16267.0
26	29574.0
27	48787.0
28	73261.0
29	102828.0
30	135984.0
31	177086.0
32	228183.0
33	301241.0
34	420756.0
35	623449.0
36	1056349.0
37	2108809.0
38	2910600.0
39	1507374.0
40	48087.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.39567032355486	20.065458492599646	14.453080478580027	24.085790705265463
2	14.35373825486303	20.99685231706791	41.740967541834834	22.908441886234225
3	17.76435407223394	26.331253385701224	30.454622955964993	25.44976958609984
4	12.29579672515608	18.36132094935659	38.928244401303374	30.414637924183957
5	13.3124345403354	37.6753686353614	35.234075189123836	13.77812163517936
6	29.592002710253862	38.043198752630154	18.836733735925762	13.528064801190226
7	26.297875067191107	33.046015245902105	23.059467752553875	17.59664193435291
8	24.491957427491037	36.52488380842065	21.0090517650845	17.974106999003816
9	25.7008865650403	17.06537961834042	21.23004254210207	36.003691274517216
10-11	24.059669346452786	26.960094216476065	28.729938719904446	20.250297717166703
12-13	25.126385031481853	24.57859666877168	28.835699612128433	21.459318687618037
14-15	22.249642846538343	25.78407186883731	26.869138828797862	25.097146455826486
16-17	21.510592057943704	28.848678662288478	27.48966395177997	22.151065327987844
18-19	21.491312250335675	27.773172527799606	29.59122930481779	21.144285917046933
20-21	22.422522972100317	26.881815284318535	29.57102844692984	21.124633296651307
22-23	22.10347537489656	26.81992182714194	29.411176690876246	21.665426107085253
24-25	21.84092811034339	26.945647294283326	29.493989777555797	21.719434817817486
26-27	21.7650159965593	27.12453218283906	29.415401625346817	21.695050195254822
28-29	21.721603711259885	27.111580794283213	29.4721218500906	21.694693644366303
30-31	22.014071571761242	26.966901929305013	29.341519298931452	21.677507200002292
32-33	21.772687389038545	27.08246204246979	29.324272933400575	21.820577635091084
34-35	21.96159211973148	27.15142282768774	29.23233835486649	21.65464669771429
36-37	21.964660576995758	27.07923597995037	29.165634938100155	21.790468504953715
38-39	21.825377473097817	27.221839919614077	29.205537365053292	21.74724524223482
40-41	22.120971125773796	27.107041761883238	29.036530534050055	21.735456578292915
42-43	22.011589072385338	27.181453741432016	28.826077599643135	21.98087958653951
44-45	22.111120689134165	27.320501397843543	28.59792162554673	21.970456287475564
46-47	22.224367726426152	27.406472572075955	28.157279452118907	22.211880249378986
48-49	22.151651903817548	27.51042262318814	28.110448103744456	22.227477369249854
50-51	22.206127069403063	27.684797453091004	27.933397453603213	22.175678023902716
52-53	22.370787231500653	27.621483207535668	27.78936586923317	22.21836369173051
54-55	22.309954236362582	27.507512366881485	27.885561007857802	22.29697238889813
56-57	22.288573758380128	27.653682178010303	27.835428416404973	22.222315647204596
58-59	22.245994736340016	27.694449588390768	27.854452506411224	22.20510316885799
60-61	22.318075902971703	27.628544383830366	27.888585620556626	22.1647940926413
62-63	22.261687395142637	27.70145706325129	27.84834926208852	22.188506279517554
64-65	22.291408202521552	27.643574673460275	27.771593554292988	22.29342356972519
66-67	22.341843607731487	27.686335137668337	27.67829481775043	22.293526436849742
68-69	22.29158832065417	27.630712672106807	27.75336151824444	22.324337488994587
70-71	22.28831772726665	27.447502080380374	27.819842032992508	22.444338159360463
72-73	22.33352708169192	27.506418248375898	27.73019968075163	22.429854989180555
74-75	22.21733824219226	27.654108046184145	27.679103353951216	22.449450357672376
76-77	22.32242749487315	27.5795949706315	27.654587883807242	22.443389650688108
78-79	22.30467458587188	27.568115372368705	27.63950705308976	22.487702988669657
80-81	22.32001810182127	27.604668379974356	27.629522702270197	22.44579081593418
82-83	22.337967815457986	27.616948235601757	27.68253222910733	22.362551719832933
84-85	22.43473078063487	27.592051367049475	27.637289151327256	22.335928700988404
86-87	22.262876825563406	27.63895656331642	27.710917975892485	22.38724863522769
88-89	22.35287968291272	27.62391891813217	27.669784306001787	22.353417092953322
90-91	22.36150462785177	27.643422576664616	27.660957342573226	22.334115452910385
92-93	22.34644825075569	27.634239617387962	27.7101849524636	22.309127179392743
94-95	22.259636698808357	27.70947987179792	27.719690257526107	22.311193171867615
96-97	22.31686117303388	27.824348019066747	27.759787508177542	22.099003299721833
98-99	23.35332569797965	29.762578794675782	27.957139868592783	18.926955638751792
100	22.45619988563628	30.7391555923535	21.64982273932511	25.15482178268511
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2410.0
1	2099.5
2	2894.5
3	4118.0
4	5157.0
5	8579.0
6	11725.0
7	14176.5
8	16297.5
9	16022.0
10	13378.0
11	10411.0
12	10394.0
13	14151.5
14	25130.0
15	41105.5
16	55005.5
17	64436.0
18	67227.5
19	63289.0
20	55844.0
21	47943.0
22	43142.0
23	44247.5
24	51036.0
25	63418.5
26	79998.0
27	97942.0
28	116822.0
29	138348.0
30	159904.0
31	181435.0
32	205099.0
33	227818.0
34	249885.5
35	271793.0
36	295290.5
37	316189.5
38	331188.5
39	344002.5
40	358537.0
41	370342.5
42	376949.0
43	383315.0
44	388565.0
45	393585.0
46	394933.0
47	393531.5
48	393636.0
49	389236.5
50	381549.5
51	371073.0
52	356837.5
53	339723.0
54	320655.5
55	300535.5
56	278906.0
57	254608.5
58	228168.0
59	201140.0
60	171053.0
61	141017.0
62	112458.5
63	87462.5
64	67000.5
65	50101.0
66	37142.0
67	28499.0
68	21937.0
69	16319.5
70	12310.0
71	9041.5
72	6580.0
73	4720.5
74	3270.0
75	2287.0
76	1587.0
77	1078.5
78	768.5
79	538.5
80	344.0
81	231.0
82	179.0
83	128.5
84	89.0
85	63.5
86	46.0
87	35.5
88	23.5
89	14.0
90	9.0
91	7.0
92	4.5
93	2.0
94	1.0
95	1.5
96	2.0
97	2.0
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0051222352046736
3	2.0407311572404785E-5
4	3.061096735860717E-5
5	0.00490795843316335
6	0.006009953258073209
7	5.101827893101197E-5
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.011192545570707032
26-27	0.0024097389395024826
28-29	0.0
30-31	4.1936123306113044E-4
32-33	0.0029981757253855538
34-35	6.372919941889249E-4
36-37	0.0028377642442221187
38-39	8.3008941308113E-5
40-41	7.831198618200667E-5
42-43	5.256673096804421E-6
44-45	0.0
46-47	0.0027523098385491833
48-49	0.0
50-51	0.0012478068024542016
52-53	0.0
54-55	3.5922394805688033E-4
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0013505443206477143
64-65	8.670023056519592E-4
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0027075347664386107
74-75	2.9335724254728917E-4
76-77	0.0030013173790467587
78-79	0.0
80-81	0.0
82-83	8.322930188193253E-4
84-85	5.141343366560414E-4
86-87	0.0016334454670432115
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0015069718025659813
96-97	9.004268700376663E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	239.0
20-21	1037.0
22-23	1914.0
24-25	2716.0
26-27	4574.0
28-29	8525.0
30-31	19708.0
32-33	25413.0
34-35	34089.0
36-37	49651.0
38-39	60082.0
40-41	63285.0
42-43	75537.0
44-45	92568.0
46-47	90742.0
48-49	77195.0
50-51	66566.0
52-53	62758.0
54-55	67273.0
56-57	69012.0
58-59	69510.0
60-61	67475.0
62-63	65540.0
64-65	69377.0
66-67	72284.0
68-69	73379.0
70-71	71379.0
72-73	69686.0
74-75	70786.0
76-77	74363.0
78-79	76640.0
80-81	78105.0
82-83	75605.0
84-85	76290.0
86-87	80770.0
88-89	86113.0
90-91	91202.0
92-93	99184.0
94-95	122938.0
96-97	334398.0
98-99	637164.0
100-101	6465337.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.54633693945246
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.22066381631817	77.1804730573182
2	6.903553846518797	11.811474868679001
3	1.6023301706529214	4.112204300007756
4	0.5607399092700105	1.9187698085524132
5	0.2726250892107775	1.1661038869886728
6	0.14116960540560633	0.7245925577782528
7	0.08314596860692182	0.49789831319234107
8	0.05628957043648298	0.3852293246989132
9	0.03432410211274313	0.2642671084032806
>10	0.12157992018647087	1.620330664762423
>50	0.003072302807078448	0.1776928329871853
>100	3.882648325245145E-4	0.05714716025605023
>500	7.829073600812757E-5	0.03877835857633955
>1k	3.914290551161119E-5	0.04503775779900744
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.0407311572404785E-5	0.0	0.0	0.0
3	0.0	2.0407311572404785E-5	0.0	0.0	0.0
4	0.0	7.142559050341674E-5	0.0	0.0	0.0
5	0.0	7.142559050341674E-5	0.0	0.0	0.0
6	4.081462314480957E-5	7.142559050341674E-5	0.0	1.0203655786202392E-5	1.0203655786202392E-5
7	4.081462314480957E-5	7.142559050341674E-5	0.0	1.0203655786202392E-5	1.0203655786202392E-5
8	4.081462314480957E-5	7.142559050341674E-5	0.0	1.0203655786202392E-5	2.0407311572404785E-5
9	4.081462314480957E-5	1.2244386943442871E-4	0.0	2.0407311572404785E-5	2.0407311572404785E-5
10-11	4.081462314480957E-5	1.2244386943442871E-4	0.0	2.0407311572404785E-5	2.0407311572404785E-5
12-13	4.081462314480957E-5	1.2244386943442871E-4	0.0	3.061096735860718E-5	2.550913946550598E-5
14-15	5.101827893101196E-5	2.499895667619586E-4	0.0	3.061096735860718E-5	3.061096735860718E-5
16-17	8.162924628961914E-5	2.652950504412622E-4	0.0	3.061096735860718E-5	5.101827893101196E-5
18-19	8.162924628961914E-5	2.806005341205658E-4	0.0	3.571279525170837E-5	6.122193471721436E-5
20-21	8.673107418272034E-5	3.061096735860718E-4	0.0	4.081462314480957E-5	6.122193471721436E-5
22-23	9.693472996892273E-5	3.2651698515847655E-4	0.0	4.081462314480957E-5	8.162924628961914E-5
24-25	1.0713838575512511E-4	3.826370919825897E-4	0.0	7.652741839651793E-5	9.693472996892273E-5
26-27	1.1224021364822632E-4	4.1834988723429806E-4	0.0	1.1734204154132751E-4	1.1224021364822632E-4
28-29	1.1224021364822632E-4	4.1834988723429806E-4	0.0	2.499895667619586E-4	1.1224021364822632E-4
30-31	1.1224021364822632E-4	4.8467364984461364E-4	0.0	7.193577329272687E-4	1.1734204154132752E-4
32-33	1.1224021364822632E-4	4.999791335239172E-4	0.0	0.0016938068605095971	1.4285118100683348E-4
34-35	1.1224021364822632E-4	5.254882729894231E-4	0.0	0.0028723291038159735	1.7856397625854185E-4
36-37	1.1224021364822632E-4	5.560992403480303E-4	0.0	0.004754903596370315	1.8366580415164307E-4
38-39	1.1224021364822632E-4	5.918120355997387E-4	0.0	0.0077955930206586276	1.9386945993784546E-4
40-41	1.1224021364822632E-4	6.530339703169531E-4	0.0	0.01121381770903643	1.9897128783094665E-4
42-43	1.1224021364822632E-4	6.836449376755602E-4	0.0	0.014698366160024547	2.0407311572404784E-4
44-45	1.1224021364822632E-4	7.448668723927746E-4	0.0	0.018662486432964177	2.0407311572404784E-4
46-47	1.1224021364822632E-4	7.754778397513818E-4	0.0	0.023198011429931137	2.0917494361714903E-4
48-49	1.1734204154132752E-4	7.80579667644483E-4	0.0	0.027493750515922347	2.2448042729645263E-4
50-51	1.275456973275299E-4	9.081253649720129E-4	0.0	0.031513990895686086	2.2448042729645263E-4
52-53	1.326475252206311E-4	9.081253649720129E-4	0.0	0.0354015837502292	2.550913946550598E-4
54-55	1.326475252206311E-4	9.183290207582153E-4	0.0	0.039381009506848134	2.8570236201366696E-4
56-57	1.326475252206311E-4	9.387363323306201E-4	0.0	0.04364103579758763	2.8570236201366696E-4
58-59	1.326475252206311E-4	9.438381602237212E-4	0.0	0.04782453466993061	2.9080418990676813E-4
60-61	1.326475252206311E-4	9.540418160099237E-4	0.0	0.051472341613497966	2.9590601779986935E-4
62-63	1.479530088999347E-4	9.591436439030248E-4	0.0	0.05512525038495843	3.2651698515847655E-4
64-65	1.530548367930359E-4	0.0010305692344064416	0.0	0.058869992058494705	3.2651698515847655E-4
66-67	1.6325849257923828E-4	0.001040772890192644	0.0	0.06301777813558598	3.316188130515778E-4
68-69	1.6325849257923828E-4	0.0010815875133374534	0.0	0.06696149109695321	3.3672064094467894E-4
70-71	1.6325849257923828E-4	0.0011224021364822632	0.0	0.0703337993342931	3.571279525170837E-4
72-73	1.8876763204474426E-4	0.0011326057922684655	0.0	0.07310919370814015	3.877389198756909E-4
74-75	1.9386945993784546E-4	0.0011632167596270725	0.0	0.07541521991582187	3.877389198756909E-4
76-77	1.9386945993784546E-4	0.0012142350385580848	0.0	0.07775695891875532	3.877389198756909E-4
78-79	1.9897128783094665E-4	0.0012397441780235907	0.0	0.07917016524514436	3.877389198756909E-4
80-81	2.0407311572404784E-4	0.0012550496617028941	0.0	0.07956300599291316	4.132480593411969E-4
82-83	2.0407311572404784E-4	0.0012652533174890967	0.0	0.07988442115017852	4.4896085459290527E-4
84-85	2.0407311572404784E-4	0.0012805588011684	0.0	0.07999155953593365	4.4896085459290527E-4
86-87	2.0917494361714903E-4	0.0012958642848477039	0.0	0.08004767964275776	4.4896085459290527E-4
88	2.1427677151025022E-4	0.0013468825637787158	0.0	0.08004767964275776	4.4896085459290527E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	15005	0.0	54.83669	1
GTATCAA	28210	0.0	39.44631	1
TCAACGC	34300	0.0	31.892181	4
ATCAACG	34660	0.0	31.585604	3
TATCAAC	35400	0.0	31.096207	2
CAACGCA	35245	0.0	31.024942	5
AACGCAG	36200	0.0	30.217817	6
ACGCAGA	41140	0.0	26.567453	7
CGCAGAG	41900	0.0	26.105974	8
TGGTATC	5950	0.0	22.712883	2
GCAGAGT	48370	0.0	22.605183	9
GTGGTAT	6345	0.0	22.377348	1
GAGTACT	30240	0.0	21.090889	12-13
CAGAGTA	47215	0.0	20.164902	10-11
GTACATG	31065	0.0	19.810324	1
TACATGG	30655	0.0	19.461441	2
AGAGTAC	44155	0.0	19.38336	10-11
GTACTTT	33030	0.0	19.199816	14-15
ACATGGG	31170	0.0	18.644915	3
AGTACTT	32275	0.0	17.700829	12-13
>>END_MODULE