FastQCFastQC Report
Fri 27 May 2016
SRR2140367_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140367_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8559076
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC133600.056.915111
GGTATCA124650.053.3340571
TATACAC178350.044.599723
TTATACA177600.043.6003272
GTATCAA238100.039.5553441
TACACAT236450.033.7480775
TCAACGC290900.031.937644
ATCAACG292000.031.7738763
ACACATC242900.031.7714186
ACATCTC244650.031.5081148
TATCAAC299050.031.1258032
CAACGCA298300.031.1028235
AACGCAG304350.030.5112676
CACATCT264100.029.0595687
CATCTCC268550.028.326119
ATACACA297550.028.1398414
TGGTATC51900.027.2159462
GTGGTAT55750.026.9294721
ACGCAGA350500.026.4443847
CGCAGAG355450.026.1118078
Produced by FastQC (version 0.11.5)