##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140367_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8559076
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.38814890766246	34.0	31.0	34.0	31.0	34.0
2	32.481731088729674	34.0	31.0	34.0	31.0	34.0
3	32.53394116374244	34.0	31.0	34.0	31.0	34.0
4	36.0535174591276	37.0	35.0	37.0	35.0	37.0
5	36.10014340332999	37.0	35.0	37.0	35.0	37.0
6	36.085529092159014	37.0	35.0	37.0	35.0	37.0
7	36.04965325696372	37.0	35.0	37.0	35.0	37.0
8	36.03404946982595	37.0	35.0	37.0	35.0	37.0
9	37.780521168406494	39.0	38.0	39.0	35.0	39.0
10-11	37.719255209324	39.0	37.0	39.0	35.0	39.0
12-13	37.704506888360385	39.0	37.0	39.0	35.0	39.0
14-15	39.044693434197804	40.0	38.0	41.0	36.0	41.0
16-17	39.0530199171032	40.0	38.0	41.0	36.0	41.0
18-19	39.10273760859233	40.0	38.5	41.0	36.0	41.0
20-21	39.13322335015552	40.0	39.0	41.0	36.0	41.0
22-23	39.08848525499869	40.0	39.0	41.0	36.0	41.0
24-25	39.05060744702578	40.0	39.0	41.0	36.0	41.0
26-27	38.99561004709525	40.0	38.5	41.0	35.0	41.0
28-29	38.96373371814663	40.0	38.0	41.0	35.0	41.0
30-31	38.883496378630056	40.0	38.0	41.0	35.0	41.0
32-33	38.769045482110016	40.0	38.0	41.0	35.0	41.0
34-35	38.68145639534424	40.0	38.0	41.0	35.0	41.0
36-37	38.628813817839195	40.0	38.0	41.0	35.0	41.0
38-39	38.55826745920836	40.0	38.0	41.0	34.5	41.0
40-41	38.54038057339736	40.0	38.0	41.0	34.5	41.0
42-43	38.487032898347834	40.0	38.0	41.0	34.0	41.0
44-45	38.447024922474455	40.0	38.0	41.0	34.0	41.0
46-47	38.375716171655306	40.0	38.0	41.0	34.0	41.0
48-49	38.313812363505335	40.0	38.0	41.0	34.0	41.0
50-51	38.23054795006398	40.0	38.0	41.0	33.5	41.0
52-53	38.15399655988689	40.0	37.0	41.0	33.0	41.0
54-55	38.02485735430393	40.0	37.0	41.0	33.0	41.0
56-57	37.898779765219984	40.0	37.0	41.0	33.0	41.0
58-59	37.76263446993253	39.0	36.0	41.0	33.0	41.0
60-61	37.55889822511186	39.0	36.0	41.0	33.0	41.0
62-63	37.34785772497851	39.0	35.5	41.0	32.5	41.0
64-65	37.1378357386425	39.0	35.0	40.0	32.0	41.0
66-67	36.86615843885424	38.0	35.0	40.0	32.0	41.0
68-69	36.62366825403383	37.5	35.0	40.0	32.0	41.0
70-71	36.33547444716395	37.0	35.0	39.5	31.0	41.0
72-73	36.016374750674686	36.5	35.0	39.0	31.0	41.0
74-75	35.69885008389981	36.0	34.5	39.0	31.0	40.5
76-77	35.370704089315375	35.5	34.0	38.5	31.0	40.0
78-79	35.07289066826801	35.0	34.0	37.0	31.0	39.0
80-81	34.78192866137259	35.0	34.0	37.0	31.0	39.0
82-83	34.497841945339616	35.0	34.0	36.5	31.0	39.0
84-85	34.26241134218107	35.0	34.0	36.0	30.5	37.0
86-87	34.0589438126491	35.0	34.0	36.0	30.0	37.0
88-89	33.90724520536348	35.0	34.0	35.0	30.0	37.0
90-91	33.80399210444687	35.0	34.0	35.0	30.0	36.0
92-93	33.71779825813368	35.0	34.0	35.0	30.0	36.0
94-95	33.62618192576176	35.0	34.0	35.0	30.0	36.0
96-97	33.630986514496776	35.0	34.0	35.0	30.0	36.0
98-99	33.712175585013505	35.0	34.0	35.0	31.0	35.0
100	32.93205676931255	34.0	32.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	27.0
20	67.0
21	287.0
22	902.0
23	2766.0
24	7047.0
25	14811.0
26	27423.0
27	45342.0
28	67687.0
29	93814.0
30	124549.0
31	161019.0
32	207693.0
33	271622.0
34	377824.0
35	554006.0
36	919251.0
37	1811049.0
38	2537121.0
39	1295709.0
40	39058.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.5425305254913	20.463447222574025	14.280747127376832	24.71327512455784
2	13.964353748999844	20.71201366195814	42.663182163180764	22.660450425861256
3	17.6155600926502	26.54448056594901	30.770626498774696	25.069332842626103
4	11.981684462988511	19.111394319384157	38.38308639067413	30.5238348269532
5	13.016669907050826	37.750471569367896	35.64887991759454	13.583978605986735
6	28.869454166673968	38.396051207182516	18.844888132531562	13.889606493611955
7	25.648709194475945	32.98622023831083	22.57373870117258	18.791331866040647
8	23.7567466394737	36.99488122316007	21.45661517668496	17.79175696068127
9	24.90279324543911	17.11299210335321	20.964435880695532	37.01977877051215
10-11	23.639549409305395	27.100857615938917	28.93218847455029	20.327404500205397
12-13	24.916083231414234	24.297061972577414	28.869640835062103	21.917213960946253
14-15	21.969742995622425	25.61728041671788	26.48778910246854	25.92518748519116
16-17	21.514787343867493	29.12704011507784	27.063704072729344	22.294468468325316
18-19	21.586348806810456	27.62487446074787	29.146201061890327	21.642575670551352
20-21	22.199902379492567	26.744706599549406	29.120804939796308	21.934586081161726
22-23	21.78298013688085	27.307335106253998	28.992862061299274	21.916822695565877
24-25	21.793376063517933	27.319142554201136	29.258938945337153	21.628542436943775
26-27	21.714060117487726	27.258544904661804	29.434211047859044	21.59318392999143
28-29	21.266915042152622	26.93991090024726	29.828018851390514	21.965155206209605
30-31	21.819933451359283	26.781674602429806	29.029310949408295	22.369080996802616
32-33	22.013449816586736	26.888123047939967	29.61315114057416	21.485275994899137
34-35	21.921695793803085	27.537189313326998	29.235481158926763	21.305633733943154
36-37	21.53537573318946	27.210481747822833	29.10941197832195	22.14473054066575
38-39	21.433956070409028	26.99397098779821	29.58347919397025	21.988593747822513
40-41	21.760108250430957	26.90343709631965	29.56230052317003	21.77415413007936
42-43	22.18495643128721	26.806646893839297	29.28193775722101	21.726458917652483
44-45	21.7311491440456	27.423004340355305	28.963225403103753	21.88262111249534
46-47	22.353849346327188	26.940778699584563	28.261586682409924	22.443785271678333
48-49	22.106644652181124	27.058049724480863	28.220884700086025	22.61442092325198
50-51	21.781945728400032	27.205215161951596	28.62087189278913	22.39196721685924
52-53	21.958536329392903	27.147943823250863	29.091319460689046	21.802200386667188
54-55	21.94110980156252	27.12244477471536	28.50780352610023	22.42864189762189
56-57	22.442572315006597	27.62806855403113	27.786812296358203	22.14254683460407
58-59	21.671545983515703	28.226252459400953	28.56436102439503	21.537840532688314
60-61	22.23692615281282	28.813243513281588	27.47440384447649	21.4754264894291
62-63	21.63729636511521	29.335522765592543	27.4542804746504	21.57290039464185
64-65	21.725380436652543	29.10718817118679	27.45454256260561	21.712888829555048
66-67	21.90265352462097	28.79845816923645	27.470091021257893	21.828797284884683
68-69	21.947076447607056	28.39975650676612	27.685537483426614	21.967629562200205
70-71	22.116082571366587	27.701533118783146	27.94600489248799	22.236379417362276
72-73	22.180607346331755	27.625522475818276	27.96580645143201	22.228063726417965
74-75	22.023630515765397	27.759753006964083	27.93286384037475	22.283752636895766
76-77	22.178110641093436	27.71409308345964	27.875538001216682	22.232258274230247
78-79	22.137225148389643	27.78028601261604	27.81120718965197	22.271281649342345
80-81	22.133194149213104	27.78850026060716	27.819295328110154	22.25901026206958
82-83	22.18666373441094	27.776833347078284	27.873363205688577	22.163139712822204
84-85	22.300572600486987	27.69599796734929	27.846552628983464	22.156876803180264
86-87	22.077294624635638	27.74400668908864	27.9577323947671	22.220966291508624
88-89	22.19223400760147	27.750506272831903	27.88961572927855	22.167643990288074
90-91	22.169909204563226	27.795567510975367	27.929127249689778	22.10539603477163
92-93	22.17307274885928	27.75002182179971	27.949237472935174	22.127667956405837
94-95	22.082495891556192	27.86684648335534	27.93696371554612	22.11369390954235
96-97	22.104984752580705	27.98582417637703	27.988195917299553	21.920995153742716
98-99	23.20423756955749	29.908961822828417	28.16786577888536	18.718934828728734
100	22.272695265368696	30.895668382802832	21.899617162140025	24.932019189688443
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2078.0
1	1743.0
2	2522.0
3	3732.0
4	4567.0
5	7427.0
6	10233.0
7	12741.0
8	15039.0
9	14801.5
10	12018.0
11	9059.5
12	8975.5
13	12318.0
14	21958.5
15	35976.5
16	48050.0
17	55918.0
18	58161.0
19	55070.0
20	49072.0
21	42790.0
22	39214.0
23	40603.0
24	46912.5
25	58082.0
26	73302.5
27	90159.0
28	107230.5
29	125310.0
30	143590.0
31	161831.5
32	181207.0
33	200636.0
34	220636.5
35	238913.5
36	257774.0
37	275452.5
38	286260.5
39	294779.0
40	307659.0
41	319229.5
42	326046.0
43	332991.0
44	339598.5
45	358573.5
46	392798.0
47	405323.5
48	392031.5
49	371020.0
50	349732.0
51	332444.0
52	312657.5
53	292795.5
54	273837.5
55	253452.0
56	232660.5
57	211441.5
58	189198.5
59	166336.0
60	141167.0
61	115432.5
62	90721.5
63	70286.5
64	53666.0
65	39909.5
66	29684.5
67	22672.5
68	17631.0
69	13291.5
70	9963.0
71	7349.0
72	5341.0
73	3956.0
74	2896.0
75	2048.0
76	1401.0
77	936.0
78	645.0
79	451.5
80	302.0
81	204.0
82	156.0
83	121.0
84	87.5
85	63.5
86	50.0
87	40.0
88	28.0
89	18.0
90	12.0
91	6.5
92	4.5
93	2.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.5
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.004907071744660288
3	1.1683504153953068E-5
4	4.673401661581227E-5
5	0.004907071744660288
6	0.005970270622670017
7	2.3367008307906136E-5
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.010875895917266985
26-27	0.002110436388553437
28-29	0.0
30-31	3.573335954560757E-4
32-33	0.0024663946389976337
34-35	5.479166999295309E-4
36-37	0.002947230023964886
38-39	7.146620178696474E-5
40-41	5.9973831018573355E-5
42-43	1.2083370665913952E-5
44-45	0.0
46-47	0.0031359834508793167
48-49	0.0
50-51	0.0014934519661295134
52-53	1.8966666904803704E-5
54-55	3.439278174487193E-4
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0017322130371753995
64-65	0.0010248736161128234
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0026005700365404625
74-75	3.463194161931043E-4
76-77	0.0030580074066507597
78-79	0.0
80-81	0.0
82-83	8.796689805626143E-4
84-85	4.8093207892435766E-4
86-87	0.0016510277236690587
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0016131245062742715
96-97	8.065451216477669E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	231.0
20-21	1001.0
22-23	1725.0
24-25	2548.0
26-27	4444.0
28-29	8908.0
30-31	19990.0
32-33	25719.0
34-35	34379.0
36-37	49959.0
38-39	58545.0
40-41	59489.0
42-43	70048.0
44-45	85239.0
46-47	82343.0
48-49	70797.0
50-51	60986.0
52-53	57181.0
54-55	61536.0
56-57	63472.0
58-59	65246.0
60-61	66377.0
62-63	73322.0
64-65	95781.0
66-67	100052.0
68-69	104778.0
70-71	87058.0
72-73	58958.0
74-75	59381.0
76-77	61963.0
78-79	65171.0
80-81	65757.0
82-83	63083.0
84-85	64365.0
86-87	67153.0
88-89	71512.0
90-91	77341.0
92-93	84144.0
94-95	104412.0
96-97	281332.0
98-99	541656.0
100-101	5481694.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.6756562186258
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.69958484357126	78.61446035073466
2	6.699052252691084	11.612895000897264
3	1.494257527495762	3.885472552659494
4	0.5253788841557373	1.8215023819043168
5	0.2240418541783939	0.9709487365674981
6	0.12145654775680514	0.6316395587321969
7	0.06633931351838608	0.4025002472609488
8	0.04163587416101022	0.288705337211335
9	0.02817507190586394	0.21978835618029524
>10	0.09736544386326954	1.2972523447665556
>50	0.002250800899330079	0.13056728807955983
>100	3.996664018164381E-4	0.058076043554469256
>500	2.0639835250297525E-5	0.012683806265757788
>1k	4.1279566096279834E-5	0.05350799518569041
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.3367008307906133E-5	0.0	1.1683504153953066E-5	0.0
3	0.0	2.3367008307906133E-5	0.0	1.1683504153953066E-5	0.0
4	0.0	2.3367008307906133E-5	0.0	3.50505124618592E-5	0.0
5	0.0	3.50505124618592E-5	0.0	3.50505124618592E-5	0.0
6	3.50505124618592E-5	3.50505124618592E-5	0.0	4.6734016615812266E-5	0.0
7	3.50505124618592E-5	3.50505124618592E-5	0.0	4.6734016615812266E-5	0.0
8	3.50505124618592E-5	5.841752076976534E-5	0.0	4.6734016615812266E-5	0.0
9	3.50505124618592E-5	1.1683504153953067E-4	0.0	7.01010249237184E-5	1.1683504153953066E-5
10-11	3.50505124618592E-5	1.402020498474368E-4	0.0	7.01010249237184E-5	1.1683504153953066E-5
12-13	3.50505124618592E-5	1.402020498474368E-4	0.0	9.346803323162453E-5	1.1683504153953066E-5
14-15	4.089226453883573E-5	1.8693606646324906E-4	0.0	1.051515373855776E-4	2.9208760384882665E-5
16-17	8.178452907767147E-5	1.8693606646324906E-4	0.0	1.2851854569348375E-4	4.6734016615812266E-5
18-19	8.178452907767147E-5	1.927778185402256E-4	0.0	1.6356905815534292E-4	4.6734016615812266E-5
20-21	8.178452907767147E-5	2.103030747711552E-4	0.0	1.927778185402256E-4	5.25757686927888E-5
22-23	8.178452907767147E-5	2.395118351560379E-4	0.0	1.9861957061720215E-4	7.01010249237184E-5
24-25	8.178452907767147E-5	2.7456234761789704E-4	0.0	2.044613226941787E-4	8.178452907767147E-5
26-27	8.178452907767147E-5	2.862458517718502E-4	0.0	2.5703709138696744E-4	8.178452907767147E-5
28-29	8.178452907767147E-5	3.0961286007975624E-4	0.0	4.907071744660288E-4	8.178452907767147E-5
30-31	9.346803323162453E-5	3.50505124618592E-4	0.0	0.0011099328946255413	8.178452907767147E-5
32-33	9.930978530860106E-5	3.621886287725451E-4	0.0	0.002377593095329449	9.346803323162453E-5
34-35	1.1099328946255415E-4	4.147643974653339E-4	0.0	0.004048334189344738	9.346803323162453E-5
36-37	1.2851854569348375E-4	4.7902367031207573E-4	0.0	0.006682964376061154	9.346803323162453E-5
38-39	1.2851854569348375E-4	4.907071744660288E-4	0.0	0.010637830532174267	9.346803323162453E-5
40-41	1.2851854569348375E-4	5.023906786199818E-4	0.0	0.015147663135600151	9.346803323162453E-5
42-43	1.2851854569348375E-4	5.37441191081841E-4	0.0	0.01926609834986861	9.346803323162453E-5
44-45	1.2851854569348375E-4	6.425927284674186E-4	0.0	0.02401544278845053	9.346803323162453E-5
46-47	1.2851854569348375E-4	7.12693753391137E-4	0.0	0.02864795218549292	9.346803323162453E-5
48-49	1.2851854569348375E-4	7.243772575450902E-4	0.0	0.03344987239276763	1.051515373855776E-4
50-51	1.2851854569348375E-4	8.937880677774096E-4	0.0	0.03787792046711584	1.051515373855776E-4
52-53	1.2851854569348375E-4	9.113133240083392E-4	0.0	0.04217160824369359	1.051515373855776E-4
54-55	1.2851854569348375E-4	9.171550760853158E-4	0.0	0.04654123879727204	1.051515373855776E-4
56-57	1.2851854569348375E-4	9.229968281622923E-4	0.0	0.05102186264031304	1.051515373855776E-4
58-59	1.2851854569348375E-4	9.229968281622923E-4	0.0	0.05549080297920009	1.051515373855776E-4
60-61	1.2851854569348375E-4	9.405220843932219E-4	0.0	0.05998311032639504	1.051515373855776E-4
62-63	1.5188555400138986E-4	9.63889092701128E-4	0.0	0.06407817853235559	1.051515373855776E-4
64-65	1.5188555400138986E-4	0.0010982493904715884	0.0	0.06807393695300754	1.051515373855776E-4
66-67	1.5188555400138986E-4	0.0010982493904715884	0.0	0.07261882006889528	1.051515373855776E-4
68-69	1.5188555400138986E-4	0.001150825159164377	0.0	0.07657952797708537	1.051515373855776E-4
70-71	1.6356905815534292E-4	0.0011566669112413536	0.0	0.07985675089226921	1.051515373855776E-4
72-73	1.8693606646324906E-4	0.0011566669112413536	0.0	0.08277762693075748	1.1683504153953067E-4
74-75	1.8693606646324906E-4	0.001215084432011119	0.0	0.08472877212446764	1.1683504153953067E-4
76-77	1.8693606646324906E-4	0.0012968689610887904	0.0	0.0868025941117943	1.226767936165072E-4
78-79	1.9861957061720215E-4	0.0013202359693966965	0.0	0.08821629811442264	1.2851854569348375E-4
80-81	1.9861957061720215E-4	0.001326077721473673	0.0	0.08862522075981098	1.2851854569348375E-4
82-83	1.9861957061720215E-4	0.0013319194735506496	0.0	0.08888809960327493	1.2851854569348375E-4
84-85	2.044613226941787E-4	0.0013436029777046028	0.0	0.08900493464481446	1.2851854569348375E-4
86-87	2.103030747711552E-4	0.001378653490166462	0.0	0.08906335216558423	1.2851854569348375E-4
88	2.3367008307906135E-4	0.0014137040026283212	0.0	0.08907503566973818	1.2851854569348375E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	13360	0.0	56.91511	1
GGTATCA	12465	0.0	53.334057	1
TATACAC	17835	0.0	44.59972	3
TTATACA	17760	0.0	43.600327	2
GTATCAA	23810	0.0	39.555344	1
TACACAT	23645	0.0	33.748077	5
TCAACGC	29090	0.0	31.93764	4
ATCAACG	29200	0.0	31.773876	3
ACACATC	24290	0.0	31.771418	6
ACATCTC	24465	0.0	31.508114	8
TATCAAC	29905	0.0	31.125803	2
CAACGCA	29830	0.0	31.102823	5
AACGCAG	30435	0.0	30.511267	6
CACATCT	26410	0.0	29.059568	7
CATCTCC	26855	0.0	28.32611	9
ATACACA	29755	0.0	28.139841	4
TGGTATC	5190	0.0	27.215946	2
GTGGTAT	5575	0.0	26.929472	1
ACGCAGA	35050	0.0	26.444384	7
CGCAGAG	35545	0.0	26.111807	8
>>END_MODULE