FastQCFastQC Report
Fri 27 May 2016
SRR2140366_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140366_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10293875
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA144250.053.1729661
GTATCAA275250.039.1274531
CTTATAC85850.034.3173141
TCAACGC339450.031.1483144
ATCAACG345750.030.556093
CAACGCA349750.030.2431985
TATCAAC352700.030.1606482
AACGCAG360550.029.4066666
TATACAC119750.027.4496963
ACGCAGA413600.025.6331027
CGCAGAG419300.025.2641898
TTATACA128300.024.1924722
GTGGTAT67850.022.810371
TGGTATC64100.022.4819642
GCAGAGT484650.021.831199
TACACAT160500.020.9053945
GAGTACT297300.020.23186512-13
ACACATC147600.019.987356
ACATCTC151750.019.8045168
GTACATG317350.019.7762931
Produced by FastQC (version 0.11.5)