##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140366_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10293875
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.40880164175299	34.0	31.0	34.0	31.0	34.0
2	32.4988362497116	34.0	31.0	34.0	31.0	34.0
3	32.54878022124807	34.0	31.0	34.0	31.0	34.0
4	36.06756503260434	37.0	35.0	37.0	35.0	37.0
5	36.113065779407655	37.0	35.0	37.0	35.0	37.0
6	36.10325324525507	37.0	36.0	37.0	35.0	37.0
7	36.07017231120448	37.0	35.0	37.0	35.0	37.0
8	36.056448130563076	37.0	35.0	37.0	35.0	37.0
9	37.802749790530775	39.0	38.0	39.0	35.0	39.0
10-11	37.74473946885891	39.0	37.5	39.0	35.0	39.0
12-13	37.73046743816104	39.0	37.0	39.0	35.0	39.0
14-15	39.083788515015	40.0	38.0	41.0	36.0	41.0
16-17	39.09974829692414	40.0	38.0	41.0	36.0	41.0
18-19	39.14321914730847	40.0	38.5	41.0	36.0	41.0
20-21	39.16804453937676	40.0	39.0	41.0	36.0	41.0
22-23	39.13044887248543	40.0	39.0	41.0	36.0	41.0
24-25	39.09559702732909	40.0	39.0	41.0	36.0	41.0
26-27	39.036940641970986	40.0	39.0	41.0	35.5	41.0
28-29	39.00208600550357	40.0	39.0	41.0	35.0	41.0
30-31	38.92086864080862	40.0	38.0	41.0	35.0	41.0
32-33	38.819543998325145	40.0	38.0	41.0	35.0	41.0
34-35	38.75194573324657	40.0	38.0	41.0	35.0	41.0
36-37	38.70083705911841	40.0	38.0	41.0	35.0	41.0
38-39	38.65852837968359	40.0	38.0	41.0	35.0	41.0
40-41	38.60978964489408	40.0	38.0	41.0	35.0	41.0
42-43	38.54894230016021	40.0	38.0	41.0	34.0	41.0
44-45	38.50888622098121	40.0	38.0	41.0	34.0	41.0
46-47	38.43493303875402	40.0	38.0	41.0	34.0	41.0
48-49	38.36817969885689	40.0	38.0	41.0	34.0	41.0
50-51	38.29027692792385	40.0	38.0	41.0	34.0	41.0
52-53	38.17874447134438	40.0	37.0	41.0	33.5	41.0
54-55	38.06587590501881	40.0	37.0	41.0	33.0	41.0
56-57	37.925718613615544	40.0	37.0	41.0	33.0	41.0
58-59	37.769430164341095	39.0	36.0	41.0	33.0	41.0
60-61	37.58573875481923	39.0	36.0	41.0	33.0	41.0
62-63	37.368638494365484	39.0	35.5	41.0	33.0	41.0
64-65	37.13562011375603	38.5	35.0	40.0	32.0	41.0
66-67	36.85707982609009	38.0	35.0	40.0	32.0	41.0
68-69	36.58718020750267	37.0	35.0	40.0	32.0	41.0
70-71	36.28043761244156	37.0	35.0	39.5	31.5	41.0
72-73	35.95717112394157	36.5	35.0	39.0	31.0	41.0
74-75	35.63746885445978	36.0	34.5	39.0	31.0	40.5
76-77	35.31387958644998	35.5	34.0	37.5	31.0	40.0
78-79	35.021186697796374	35.0	34.0	37.0	31.0	39.0
80-81	34.74199903162386	35.0	34.0	37.0	31.0	39.0
82-83	34.46722059509817	35.0	34.0	36.0	31.0	38.5
84-85	34.2423098166489	35.0	34.0	36.0	31.0	37.0
86-87	34.048472475142376	35.0	34.0	35.5	30.5	37.0
88-89	33.905924649410736	35.0	34.0	35.0	30.0	36.5
90-91	33.80834178882331	35.0	34.0	35.0	30.0	36.0
92-93	33.72728354568647	35.0	34.0	35.0	30.0	36.0
94-95	33.637637852145275	35.0	34.0	35.0	30.0	36.0
96-97	33.644968998719634	35.0	34.0	35.0	30.5	35.5
98-99	33.72427836566572	35.0	34.0	35.0	31.0	35.0
100	32.95034038662335	34.0	32.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	20.0
20	87.0
21	294.0
22	1036.0
23	3174.0
24	8108.0
25	17205.0
26	32256.0
27	52935.0
28	79136.0
29	110529.0
30	145705.0
31	188615.0
32	243546.0
33	319637.0
34	448617.0
35	664176.0
36	1125549.0
37	2243674.0
38	3026168.0
39	1534974.0
40	48432.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.21467377445325	20.168954839649793	14.294723804203958	24.321647581693
2	14.379950997631488	20.87764174644286	41.71039056170082	23.032016694224833
3	17.849693905326607	26.14236389526431	30.4523447634972	25.55559743591188
4	12.4004492382796	18.498700537183833	38.42509555930816	30.6757546652284
5	13.464965802701684	37.59241032833118	35.1127625321846	13.829861336782548
6	29.619712686056033	37.79178045460772	18.880715263468268	13.70779159586798
7	26.527603914797414	32.85069963024581	22.62911718493324	17.992579270023526
8	24.549792959405472	36.44961688382654	20.968935410620393	18.031654746147588
9	25.519631819892897	17.111622202523343	21.005481414919068	36.363264562664696
10-11	24.159114036259428	26.803740088159223	28.651601073453875	20.385544802127477
12-13	25.313552962319825	24.21399618705298	28.694777234034802	21.77767361659239
14-15	22.405027261356874	25.55888331653532	26.45870966958507	25.577379752522738
16-17	21.819858896673992	28.763905720634842	27.055860888149507	22.360374494541656
18-19	21.84052166943935	27.61432890918143	29.03423152117157	21.510917900207648
20-21	22.674024018160026	26.658882050187994	29.012924620080987	21.654169311570993
22-23	22.3706641522439	26.804394112158263	28.83238346961679	21.992558265981046
24-25	22.220380437014324	26.990166762230533	28.886729286391816	21.902723514363327
26-27	22.20376291778657	27.056103712483203	28.8465849525285	21.893548417201732
28-29	22.068523341664825	27.050337760021915	28.8581719171665	22.02296698114676
30-31	22.61893254741717	26.83803451552048	28.74014108878697	21.80289184827538
32-33	21.960152251392234	27.208319112265734	28.78837772860279	22.043150907739246
34-35	22.269647270777682	27.10895964443839	28.80184988029392	21.81954320449001
36-37	22.16956184902368	27.082378683927267	28.6940995506067	22.053959916442352
38-39	22.092348211709023	26.975614739379868	28.83760913614158	22.094427912769532
40-41	22.363969765458307	26.865198949683034	28.705271876541328	22.06555940831733
42-43	22.38594241040243	26.889896081202018	28.491878707051203	22.232282801344343
44-45	22.35360423732402	27.119801377202986	28.263225979157685	22.263368406315305
46-47	22.634648043531	27.02270229826239	27.761008642184215	22.581641016022395
48-49	22.469514128669026	27.160855338298155	27.701898253708222	22.667732279324593
50-51	22.393458322437382	27.302080596384126	27.758191561524615	22.546269519653876
52-53	22.571997695785527	27.21594116264673	27.83470409520188	22.377357046365866
54-55	22.52041809425231	27.150501899462775	27.656019186246212	22.673060820038707
56-57	22.65763117462015	27.419175100237105	27.427494521502126	22.49569920364062
58-59	22.41448774475099	27.590087052543833	27.688056617029005	22.30736858567618
60-61	22.647923562463628	27.722081238093526	27.35275499957509	22.277240199867755
62-63	22.44345247629747	27.959706376945842	27.305396537185324	22.291444609571364
64-65	22.474779769177648	27.805033032090282	27.30068592324094	22.419501275491132
66-67	22.546718175673693	27.771194219599664	27.182025523803983	22.50006208092266
68-69	22.524542087294712	27.609210577408454	27.33552590226968	22.53072143302715
70-71	22.59108500570724	27.271874047581658	27.438090725257343	22.698950221453764
72-73	22.65310708857079	27.279572454827637	27.370229795783878	22.697090660817693
74-75	22.49502229805832	27.39799116861002	27.385350754533277	22.721635778798387
76-77	22.636063953820475	27.354010857686482	27.339298498231706	22.67062669026134
78-79	22.592728881997377	27.369404796681646	27.31344961748255	22.724416703838425
80-81	22.611209237701004	27.406043363310346	27.274175332764194	22.708572066224455
82-83	22.623127936058832	27.3983907263817	27.34445976517498	22.634021572384484
84-85	22.70826919279723	27.337140498573582	27.3388944433823	22.61569586524689
86-87	22.58154227833459	27.412725736855325	27.383313927383696	22.62241805742639
88-89	22.632440261812516	27.39110135053347	27.318171890877935	22.658286496776082
90-91	22.647333594727915	27.434627178404043	27.33913805282121	22.57890117404683
92-93	22.62041530539627	27.400593905752917	27.415293419824298	22.56369736902651
94-95	22.556779843248336	27.461399524984103	27.40936076380961	22.572459867957956
96-97	22.59296114310892	27.56899963491325	27.426362759012353	22.411676462965477
98-99	23.695625555350734	29.54895411173237	27.548993911794422	19.206426421122476
100	22.713163272049865	30.46970862642477	21.26961010503627	25.547517996489095
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1707.0
1	1543.0
2	2538.5
3	3831.5
4	4901.0
5	8372.5
6	11496.0
7	13552.5
8	14826.5
9	14099.5
10	11764.5
11	9291.0
12	9420.0
13	12802.0
14	22998.5
15	38574.0
16	52475.0
17	61750.5
18	64665.0
19	61255.0
20	54561.5
21	47205.5
22	42130.5
23	42818.5
24	49348.5
25	61452.0
26	77635.5
27	95938.0
28	115789.0
29	137469.0
30	158439.5
31	180010.0
32	204752.5
33	228924.5
34	251995.0
35	272893.5
36	295575.0
37	318439.0
38	334953.5
39	347393.0
40	361686.5
41	375184.0
42	384060.0
43	393996.5
44	414159.5
45	436093.0
46	437844.0
47	431713.0
48	428045.0
49	422221.5
50	416821.0
51	407185.5
52	392067.5
53	373813.0
54	355348.5
55	335840.5
56	313237.0
57	286297.0
58	255048.5
59	223073.5
60	189387.5
61	155884.0
62	124938.5
63	96684.0
64	73193.0
65	54886.0
66	40796.5
67	30752.0
68	23436.0
69	17494.5
70	13106.0
71	9753.5
72	7233.0
73	5221.5
74	3638.0
75	2493.5
76	1727.5
77	1140.5
78	775.0
79	565.5
80	413.0
81	298.0
82	218.0
83	159.5
84	113.5
85	80.5
86	59.5
87	49.5
88	33.5
89	18.0
90	13.0
91	9.5
92	6.0
93	2.5
94	1.0
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.004808684776141152
3	9.714514699275054E-6
4	5.828708819565033E-5
5	0.005032118614224478
6	0.005877281393061408
7	8.743063229347548E-5
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.010796846640511604
26-27	0.0020414507832463382
28-29	0.0
30-31	3.262574424435611E-4
32-33	0.002415530484897493
34-35	4.4531129535036774E-4
36-37	0.0030115533602057564
38-39	3.951924443946941E-5
40-41	2.4855096032883094E-5
42-43	1.5014127543311879E-5
44-45	0.0
46-47	0.00300194249633893
48-49	0.0
50-51	0.0013997776323621952
52-53	1.0442373942141835E-5
54-55	4.890348655041064E-4
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0016397793530277711
64-65	9.748812000458961E-4
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.002730018911792804
74-75	2.5292260694688727E-4
76-77	0.0030154498311783014
78-79	0.0
80-81	0.0
82-83	7.521029335237706E-4
84-85	4.7615457086388487E-4
86-87	0.001625507240447588
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.001259244178632416
96-97	8.869481535761164E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	287.0
20-21	1048.0
22-23	1927.0
24-25	2832.0
26-27	5049.0
28-29	9805.0
30-31	20722.0
32-33	26530.0
34-35	36182.0
36-37	52027.0
38-39	63252.0
40-41	65462.0
42-43	78667.0
44-45	95751.0
46-47	92898.0
48-49	79392.0
50-51	69311.0
52-53	66571.0
54-55	71961.0
56-57	74265.0
58-59	76358.0
60-61	75971.0
62-63	77244.0
64-65	87482.0
66-67	90990.0
68-69	93567.0
70-71	86860.0
72-73	74545.0
74-75	75433.0
76-77	78520.0
78-79	82443.0
80-81	83560.0
82-83	81007.0
84-85	82099.0
86-87	86263.0
88-89	92164.0
90-91	97548.0
92-93	105634.0
94-95	131018.0
96-97	352180.0
98-99	665937.0
100-101	6703113.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.48305386571968
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.96377149404525	74.26967327734954
2	7.517745808527672	12.55208756564208
3	1.9337950881625794	4.843173585310223
4	0.6993142621786197	2.3352356087409487
5	0.32922964486144163	1.3742548088079742
6	0.16967112182254696	0.8498798041541262
7	0.1088057714704066	0.6358406656395598
8	0.070005363081112	0.4675409197591789
9	0.044524701782235256	0.33453522695173055
>10	0.15905845320227643	2.026287813944813
>50	0.003360230470751217	0.18989143349116486
>100	6.769822255500946E-4	0.09582803472436541
>500	4.107816949065556E-5	0.02577125548427762
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.942902939855011E-5	0.0	1.942902939855011E-5	0.0
3	0.0	1.942902939855011E-5	0.0	2.9143544097825163E-5	0.0
4	9.714514699275054E-6	4.857257349637527E-5	0.0	2.9143544097825163E-5	0.0
5	9.714514699275054E-6	4.857257349637527E-5	0.0	3.885805879710022E-5	0.0
6	1.1657417639130065E-4	4.857257349637527E-5	0.0	3.885805879710022E-5	0.0
7	1.1657417639130065E-4	4.857257349637527E-5	0.0	6.800160289492538E-5	0.0
8	1.1657417639130065E-4	5.828708819565033E-5	0.0	6.800160289492538E-5	0.0
9	1.1657417639130065E-4	8.74306322934755E-5	0.0	7.771611759420044E-5	0.0
10-11	1.262886910905757E-4	8.74306322934755E-5	0.0	9.714514699275055E-5	0.0
12-13	1.3600320578985076E-4	8.74306322934755E-5	0.0	1.068596616920256E-4	0.0
14-15	1.5543223518840087E-4	1.3600320578985076E-4	0.0	1.068596616920256E-4	1.942902939855011E-5
16-17	2.4286286748187636E-4	1.3600320578985076E-4	0.0	1.1657417639130065E-4	1.942902939855011E-5
18-19	2.4286286748187636E-4	1.651467498876759E-4	0.0	1.74861264586951E-4	2.9143544097825163E-5
20-21	2.5257738218115145E-4	1.9914755133513862E-4	0.0	2.0400480868477613E-4	3.400080144746269E-5
22-23	2.720064115797015E-4	2.2829109543296376E-4	0.0	2.1857658073368873E-4	3.885805879710022E-5
24-25	2.865781836286141E-4	2.962926983278892E-4	0.0	2.4286286748187636E-4	4.371531614673775E-5
26-27	2.914354409782516E-4	3.1572172772643926E-4	0.0	2.67149154230064E-4	4.857257349637527E-5
28-29	2.914354409782516E-4	3.2057898507607677E-4	0.0	5.002975070126653E-4	4.857257349637527E-5
30-31	2.914354409782516E-4	3.934378453206397E-4	0.0	0.001418319146094158	4.857257349637527E-5
32-33	3.060072130271642E-4	4.0800961736955227E-4	0.0	0.003283505968354968	5.828708819565033E-5
34-35	3.3029349977535186E-4	4.711539629148402E-4	0.0	0.0058432805916139455	5.828708819565033E-5
36-37	3.3029349977535186E-4	5.24583793760853E-4	0.0	0.009510509890590279	5.828708819565033E-5
38-39	3.3029349977535186E-4	5.44012823159403E-4	0.0	0.01535864773955386	6.314434554528785E-5
40-41	3.59437043873177E-4	5.731563672572282E-4	0.0	0.02144964845599932	6.800160289492538E-5
42-43	3.8372333062136464E-4	6.071571687046909E-4	0.0	0.027749511238479192	6.800160289492538E-5
44-45	3.9829510267027724E-4	7.140168303967165E-4	0.0	0.035015968233536934	7.28588602445629E-5
46-47	4.1772413206882735E-4	8.160192347391045E-4	0.0	0.042870153367900815	7.771611759420044E-5
48-49	4.1772413206882735E-4	8.160192347391045E-4	0.0	0.05088948525215237	9.714514699275054E-5
50-51	4.274386467681024E-4	9.083071243822175E-4	0.0	0.058272516423601414	1.0200240434238807E-4
52-53	4.3715316146737747E-4	9.131643817318551E-4	0.0	0.06558754599215552	1.1171691904166312E-4
54-55	4.5658219086592753E-4	9.228788964311301E-4	0.0	0.072509137715389	1.2143143374093818E-4
56-57	4.662967055652026E-4	9.423079258296803E-4	0.0	0.0775169700428653	1.262886910905757E-4
58-59	4.7601122026447764E-4	9.617369552282304E-4	0.0	0.08214107903972023	1.262886910905757E-4
60-61	4.8572573496375273E-4	9.95737756675693E-4	0.0	0.08688661947031609	1.262886910905757E-4
62-63	5.197265364112154E-4	0.0010540248448713434	0.0	0.09139415429077971	1.3600320578985076E-4
64-65	5.44012823159403E-4	0.0011463127345144564	0.0	0.09536253354543356	1.3600320578985076E-4
66-67	5.53727337858678E-4	0.0011754562786122815	0.0	0.09943291520442982	1.3600320578985076E-4
68-69	5.53727337858678E-4	0.0011997425653604691	0.0	0.10392587825284452	1.3600320578985076E-4
70-71	5.731563672572282E-4	0.001224028852108657	0.0	0.10800111716919042	1.3600320578985076E-4
72-73	5.974426540054159E-4	0.0012580296535561195	0.0	0.1111437626744059	1.457177204891258E-4
74-75	6.022999113550534E-4	0.0013308885138006825	0.0	0.11356753409187502	1.457177204891258E-4
76-77	6.022999113550534E-4	0.00137460382994742	0.0	0.11588930310500176	1.457177204891258E-4
78-79	6.022999113550534E-4	0.0013794610872970576	0.0	0.11737562385399085	1.457177204891258E-4
80-81	6.071571687046909E-4	0.0013891756019963329	0.0	0.11795363747859772	1.457177204891258E-4
82-83	6.120144260543285E-4	0.0013891756019963329	0.0	0.11825478743427523	1.457177204891258E-4
84-85	6.16871683403966E-4	0.0014134618887445204	0.0	0.11837621886801618	1.457177204891258E-4
86-87	6.411579701521535E-4	0.001457177204891258	0.0	0.1184830785297082	1.457177204891258E-4
88	6.508724848514287E-4	0.0014960352636883584	0.0	0.11848793578705784	1.457177204891258E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14425	0.0	53.172966	1
GTATCAA	27525	0.0	39.127453	1
CTTATAC	8585	0.0	34.317314	1
TCAACGC	33945	0.0	31.148314	4
ATCAACG	34575	0.0	30.55609	3
CAACGCA	34975	0.0	30.243198	5
TATCAAC	35270	0.0	30.160648	2
AACGCAG	36055	0.0	29.406666	6
TATACAC	11975	0.0	27.449696	3
ACGCAGA	41360	0.0	25.633102	7
CGCAGAG	41930	0.0	25.264189	8
TTATACA	12830	0.0	24.192472	2
GTGGTAT	6785	0.0	22.81037	1
TGGTATC	6410	0.0	22.481964	2
GCAGAGT	48465	0.0	21.83119	9
TACACAT	16050	0.0	20.905394	5
GAGTACT	29730	0.0	20.231865	12-13
ACACATC	14760	0.0	19.98735	6
ACATCTC	15175	0.0	19.804516	8
GTACATG	31735	0.0	19.776293	1
>>END_MODULE