FastQCFastQC Report
Fri 27 May 2016
SRR2140365_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140365_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10360366
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA146900.048.842221
GTATCAA269400.038.8863451
CTTATAC86350.035.3788571
TCAACGC325200.031.6119564
ATCAACG328050.031.3502523
CAACGCA333150.030.8830645
TATCAAC340300.030.460372
AACGCAG344500.029.8770336
TATACAC123650.028.3079413
ACGCAGA390550.026.363557
CGCAGAG397200.025.8794448
TTATACA133750.024.4587482
GTGGTAT65700.022.4743671
GCAGAGT462350.022.2052279
TGGTATC67600.021.026732
GAGTACT269550.020.73585912-13
TACACAT165550.020.4513595
ACACATC152150.020.2720816
GTACATG333200.020.2090341
TACATGG327600.020.1400322
Produced by FastQC (version 0.11.5)