##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140365_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10360366
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.40414209304961	34.0	31.0	34.0	31.0	34.0
2	32.493923187655724	34.0	31.0	34.0	31.0	34.0
3	32.54868563523721	34.0	31.0	34.0	31.0	34.0
4	36.06735283290185	37.0	35.0	37.0	35.0	37.0
5	36.11353546776243	37.0	35.0	37.0	35.0	37.0
6	36.10421407892347	37.0	36.0	37.0	35.0	37.0
7	36.07278796907368	37.0	35.0	37.0	35.0	37.0
8	36.05865304372452	37.0	35.0	37.0	35.0	37.0
9	37.805967569099394	39.0	38.0	39.0	35.0	39.0
10-11	37.7467201930897	39.0	37.5	39.0	35.0	39.0
12-13	37.73485478215731	39.0	37.0	39.0	35.0	39.0
14-15	39.088122417682925	40.0	38.0	41.0	36.0	41.0
16-17	39.10029471931783	40.0	38.0	41.0	36.0	41.0
18-19	39.14206674744888	40.0	38.5	41.0	36.0	41.0
20-21	39.16715585738942	40.0	39.0	41.0	36.0	41.0
22-23	39.129970384229495	40.0	39.0	41.0	36.0	41.0
24-25	39.09590352657332	40.0	39.0	41.0	36.0	41.0
26-27	39.03634214747271	40.0	39.0	41.0	35.5	41.0
28-29	38.99800573095866	40.0	39.0	41.0	35.0	41.0
30-31	38.919752641372924	40.0	38.0	41.0	35.0	41.0
32-33	38.8246862641225	40.0	38.0	41.0	35.0	41.0
34-35	38.761421134164365	40.0	38.0	41.0	35.0	41.0
36-37	38.71196907469886	40.0	38.0	41.0	35.0	41.0
38-39	38.66542855393614	40.0	38.0	41.0	35.0	41.0
40-41	38.62248010347382	40.0	38.0	41.0	35.0	41.0
42-43	38.5646471879118	40.0	38.0	41.0	34.5	41.0
44-45	38.52553169991012	40.0	38.0	41.0	34.0	41.0
46-47	38.45416433640446	40.0	38.0	41.0	34.0	41.0
48-49	38.386034180060975	40.0	38.0	41.0	34.0	41.0
50-51	38.304780507749996	40.0	38.0	41.0	34.0	41.0
52-53	38.19918608306806	40.0	37.0	41.0	34.0	41.0
54-55	38.07397776898777	40.0	37.0	41.0	33.0	41.0
56-57	37.93685534395152	40.0	37.0	41.0	33.0	41.0
58-59	37.789105794870736	39.5	36.0	41.0	33.0	41.0
60-61	37.60527549497847	39.0	36.0	41.0	33.0	41.0
62-63	37.39106853475069	39.0	35.5	41.0	33.0	41.0
64-65	37.160137031385716	39.0	35.0	40.0	32.0	41.0
66-67	36.882675071606	38.0	35.0	40.0	32.0	41.0
68-69	36.613924585648846	37.0	35.0	40.0	32.0	41.0
70-71	36.30500426394434	37.0	35.0	39.5	32.0	41.0
72-73	35.97829540339524	36.5	35.0	39.0	31.0	41.0
74-75	35.657424983179226	36.0	34.5	39.0	31.0	40.5
76-77	35.33016650318771	35.5	34.0	37.5	31.0	40.0
78-79	35.034037284700986	35.0	34.0	37.0	31.0	39.0
80-81	34.754173711068276	35.0	34.0	37.0	31.0	39.0
82-83	34.47873295761694	35.0	34.0	36.0	31.0	38.5
84-85	34.25074242392552	35.0	34.0	36.0	31.0	37.0
86-87	34.055463462027404	35.0	34.0	35.5	30.5	37.0
88-89	33.914192106985084	35.0	34.0	35.0	30.0	36.5
90-91	33.81437530065454	35.0	34.0	35.0	30.5	36.0
92-93	33.73397044416832	35.0	34.0	35.0	30.5	36.0
94-95	33.64489342764884	35.0	34.0	35.0	30.0	36.0
96-97	33.650111818684806	35.0	34.0	35.0	30.5	35.5
98-99	33.73235299448977	35.0	34.0	35.0	31.0	35.0
100	32.947167399128276	34.0	32.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	3.0
19	28.0
20	97.0
21	289.0
22	1102.0
23	3309.0
24	8149.0
25	17507.0
26	32008.0
27	52129.0
28	78488.0
29	108522.0
30	144999.0
31	186759.0
32	242406.0
33	318152.0
34	448045.0
35	661977.0
36	1122708.0
37	2252401.0
38	3062729.0
39	1568536.0
40	50022.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.12056465958828	20.20112996008056	14.173958719218993	24.504346661112166
2	14.329420175016336	21.020667031987873	41.76731420284477	22.88259859015102
3	17.905575720546523	26.23664320706848	30.412982554186073	25.444798518198926
4	12.387813974399922	18.5097060825788	38.57509184768597	30.527388095335304
5	13.422508290110729	37.657411717101674	35.1677433466275	13.752336646160101
6	29.47358219596325	37.89713194895846	18.934743204399965	13.69454265067833
7	26.463052724625925	32.804310430199415	22.713491174725817	18.01914567044884
8	24.461027728170993	36.52644124734589	21.096889820301715	17.915641204181394
9	25.606730495814528	17.09968547443208	20.999547699376645	36.29403633037674
10-11	24.142786075318188	26.81117153583184	28.663750875210393	20.382291513639576
12-13	25.207936669418824	24.22025438097457	28.69195451203172	21.87985443757489
14-15	22.33467427695122	25.621604487717903	26.433154002474428	25.610567232856447
16-17	21.85092206201982	28.806047971664324	27.001256519316012	22.341773446999845
18-19	21.848899932685775	27.701820572748105	28.95207563130492	21.497203863261202
20-21	22.67690832924023	26.764701013469573	28.890255911093405	21.66813474619679
22-23	22.328804786445716	26.911395418524386	28.713562587318886	22.046237207711012
24-25	22.208172127284428	27.029373496829894	28.80568232642533	21.956772049460348
26-27	22.154657576983965	27.188970871610806	28.763692719013477	21.892678832391752
28-29	21.864233518444916	27.044679260052984	28.914643707514838	22.17644351398726
30-31	22.414171391171696	26.815942689278106	28.911498521894313	21.858387397655882
32-33	22.002532939290383	27.236036067837468	28.6995094084793	22.06192158439285
34-35	22.126628685996604	27.237359932056087	28.89054605747049	21.745465324476818
36-37	22.317374574945244	27.059005096873207	28.611111938404942	22.01250838977661
38-39	22.058400378366933	27.107410916624534	28.750500358057995	22.083688346950538
40-41	22.35125795464341	26.931202961139984	28.664864457700318	22.052674626516293
42-43	22.33746904907705	26.970499994500045	28.480513318322693	22.211517638100208
44-45	22.275581874972637	27.221865550342812	28.268740468761234	22.233812105923313
46-47	22.571936334992042	27.055366600395438	27.76098683480532	22.611710229807205
48-49	22.44110444871888	27.18890659220523	27.779117628102135	22.59087133097375
50-51	22.324882487577703	27.374112268885355	27.813308817380584	22.48769642615636
52-53	22.52968595747257	27.33898321480516	27.831759863256174	22.2995709644661
54-55	22.433705310413654	27.28510181618196	27.690165651242864	22.59102722216152
56-57	22.61769005921336	27.556479778952852	27.399959665320434	22.425870496513355
58-59	22.329181040234904	27.777179101162208	27.684286518882722	22.209353339720163
60-61	22.590940881603753	27.882176767653682	27.369028754799434	22.15785359594313
62-63	22.352809536169005	28.08559943169439	27.30318616995674	22.258404862179866
64-65	22.384290740329057	27.944789246024566	27.336633854788555	22.334286158857818
66-67	22.49036248905432	27.874746224017017	27.21941097410135	22.41548031282732
68-69	22.450107370171146	27.723655598981463	27.347120220077947	22.47911681076945
70-71	22.49975246334336	27.345665845316464	27.49097410625122	22.663607585088958
72-73	22.545719162438004	27.36463575711683	27.461288689384293	22.628356391060873
74-75	22.44797057947741	27.520191586221728	27.398236394406343	22.63360143989452
76-77	22.53517020318234	27.44694644538565	27.37071087631332	22.647172475118687
78-79	22.514848120505622	27.445512027634404	27.334341770694465	22.705298081165513
80-81	22.52243376585293	27.496988135507568	27.328916387363634	22.65166171127587
82-83	22.568460999080976	27.475431930853862	27.395435123285157	22.56067194678001
84-85	22.66299514201998	27.419292843497622	27.38223809012817	22.535473924354225
86-87	22.49278358928713	27.488082522139372	27.433848394168642	22.585285494404854
88-89	22.58220603876491	27.47795110655749	27.364081007202312	22.575761847475288
90-91	22.600116542586658	27.470254355136248	27.410546665019098	22.519082437258
92-93	22.63489552032607	27.43507406493963	27.41750938271136	22.512521032022946
94-95	22.502204581415803	27.555910486483114	27.41859683462277	22.523288097478314
96-97	22.50674328001352	27.642447184219986	27.509048944842498	22.341760590923997
98-99	23.573861336989292	29.61929626566529	27.6501700791272	19.156672318218217
100	22.69319789208943	30.581064449942698	21.27357173752268	25.45216592044519
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1861.0
1	1717.5
2	2578.0
3	3659.0
4	4739.0
5	8215.5
6	11193.0
7	12978.0
8	13932.0
9	13007.5
10	10900.0
11	8539.0
12	8600.5
13	12086.5
14	22071.0
15	36946.5
16	50080.5
17	58838.5
18	61486.5
19	58345.0
20	51916.5
21	44387.5
22	39798.0
23	41151.5
24	48037.0
25	60782.0
26	78934.5
27	98896.5
28	117767.0
29	138663.5
30	159046.0
31	179509.5
32	204523.5
33	229904.5
34	255332.5
35	279608.5
36	304616.0
37	327807.5
38	343435.0
39	356029.5
40	372370.0
41	387536.0
42	396471.0
43	407352.0
44	429571.0
45	453164.5
46	455029.5
47	449787.0
48	445524.0
49	434649.0
50	426871.5
51	416626.0
52	399631.5
53	379265.0
54	357087.0
55	333449.5
56	308438.5
57	280638.5
58	250829.5
59	219784.0
60	185094.5
61	151343.5
62	120823.0
63	93591.0
64	70939.5
65	52898.5
66	38787.5
67	28969.0
68	22300.0
69	16499.0
70	11903.0
71	8567.0
72	6189.0
73	4459.0
74	3121.5
75	2107.0
76	1423.0
77	926.5
78	620.0
79	436.0
80	299.0
81	210.0
82	153.0
83	108.0
84	81.5
85	58.5
86	38.0
87	24.5
88	16.5
89	13.5
90	10.0
91	6.0
92	4.0
93	1.5
94	0.0
95	0.5
96	2.0
97	2.5
98	1.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.005144605895197139
3	9.652168658906452E-6
4	6.756518061234516E-5
5	0.004169736860647587
6	0.0062546052909713806
7	7.721734927125161E-5
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.010799954617982383
26-27	0.0021104283314716974
28-29	0.0
30-31	3.531785147092078E-4
32-33	0.0027977048928512966
34-35	5.397438958976984E-4
36-37	0.0029976974851612754
38-39	5.401310760633696E-5
40-41	2.471306524896708E-5
42-43	9.954664962560755E-6
44-45	0.0
46-47	0.002991954077663217
48-49	0.0
50-51	0.0015072747687572086
52-53	1.0401616827319639E-5
54-55	5.030586753490402E-4
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.001551952505478094
64-65	0.001013226050403231
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0025253911799513196
74-75	3.228452629750502E-4
76-77	0.003119159577367844
78-79	0.0
80-81	0.0
82-83	7.98101454656916E-4
84-85	4.5469467707129335E-4
86-87	0.00169095867676847
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.001560284794528223
96-97	9.475245132487615E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	283.0
20-21	1049.0
22-23	1956.0
24-25	2812.0
26-27	4889.0
28-29	9522.0
30-31	21698.0
32-33	27115.0
34-35	37247.0
36-37	54431.0
38-39	66498.0
40-41	68556.0
42-43	81173.0
44-45	98425.0
46-47	96273.0
48-49	83531.0
50-51	73528.0
52-53	71067.0
54-55	76911.0
56-57	80950.0
58-59	83815.0
60-61	83638.0
62-63	83764.0
64-65	92098.0
66-67	96420.0
68-69	100048.0
70-71	91745.0
72-73	78567.0
74-75	79719.0
76-77	83788.0
78-79	86906.0
80-81	88226.0
82-83	85254.0
84-85	85367.0
86-87	90169.0
88-89	96228.0
90-91	102236.0
92-93	108532.0
94-95	132999.0
96-97	350071.0
98-99	665199.0
100-101	6637663.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.25018286545878
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.79786202749945	73.92438251851108
2	7.6363907782181055	12.714618574375208
3	1.9459997694419595	4.860145099865513
4	0.735857592160686	2.450411164412532
5	0.3319521198497917	1.3817537340035924
6	0.16743299952172624	0.8363296696737592
7	0.11199471641658845	0.6526506435152332
8	0.0708833573038311	0.4720841966129274
9	0.04486013678241584	0.3361153131455052
>10	0.15216596838022034	1.9399075914235244
>50	0.0036028171185266957	0.2014374391048052
>100	8.755646633639769E-4	0.11874435235034163
>500	6.107756419384426E-5	0.033914799154686925
>1k	6.107507923316993E-5	0.07750490385124735
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	9.652168658906452E-6	0.0
2	0.0	2.8956505976719356E-5	0.0	9.652168658906452E-6	0.0
3	0.0	2.8956505976719356E-5	0.0	9.652168658906452E-6	9.652168658906452E-6
4	0.0	4.8260843294532256E-5	0.0	9.652168658906452E-6	9.652168658906452E-6
5	0.0	4.8260843294532256E-5	0.0	9.652168658906452E-6	9.652168658906452E-6
6	0.0	4.8260843294532256E-5	0.0	9.652168658906452E-6	3.8608674635625806E-5
7	0.0	4.8260843294532256E-5	0.0	9.652168658906452E-6	3.8608674635625806E-5
8	0.0	6.756518061234516E-5	0.0	1.9304337317812903E-5	3.8608674635625806E-5
9	0.0	1.9304337317812902E-4	0.0	6.756518061234516E-5	3.8608674635625806E-5
10-11	0.0	2.026955418370355E-4	0.0	8.686951793015807E-5	3.8608674635625806E-5
12-13	0.0	2.026955418370355E-4	0.0	8.686951793015807E-5	4.3434758965079035E-5
14-15	0.0	2.6543463811992744E-4	0.0	8.686951793015807E-5	6.273909628289193E-5
16-17	0.0	2.992172284261E-4	0.0	1.0617385524797097E-4	6.756518061234516E-5
18-19	0.0	3.1852156574391287E-4	0.0	1.1582602390687743E-4	6.756518061234516E-5
20-21	0.0	3.474780717206323E-4	0.0	1.4960861421305002E-4	6.756518061234516E-5
22-23	0.0	3.8608674635625805E-4	0.0	1.689129515308629E-4	8.686951793015807E-5
24-25	0.0	4.1504325233297746E-4	0.0	1.9786945750758226E-4	9.652168658906451E-5
26-27	0.0	4.3434758965079035E-4	0.0	2.654346381199274E-4	9.652168658906451E-5
28-29	0.0	4.3434758965079035E-4	0.0	4.970866859336823E-4	9.652168658906451E-5
30-31	0.0	5.019127702631355E-4	0.0	0.0013513036122469032	9.652168658906451E-5
32-33	0.0	5.019127702631355E-4	0.0	0.0030211287902377195	9.652168658906451E-5
34-35	0.0	6.032605411816533E-4	0.0	0.005434170954964332	1.351303612246903E-4
36-37	0.0	7.04608312100171E-4	0.0	0.008556647516120569	1.351303612246903E-4
38-39	0.0	7.287387337474371E-4	0.0	0.013739862085953333	1.3995644555414355E-4
40-41	0.0	7.528691553947033E-4	0.0	0.019246424305859468	1.8339120451922258E-4
42-43	0.0	7.963039143597823E-4	0.0	0.024362073695079884	1.8339120451922258E-4
44-45	0.0	8.928256009488468E-4	0.0	0.03016302705908266	1.8339120451922258E-4
46-47	0.0	9.700429502200984E-4	0.0	0.036876110361352105	1.9304337317812902E-4
48-49	0.0	9.941733718673645E-4	0.0	0.04372432402484623	2.2199987915484838E-4
50-51	0.0	0.0011437819860804146	0.0	0.050254016122596445	2.2199987915484838E-4
52-53	0.0	0.0011582602390687743	0.0	0.05657136050985072	2.461303008021145E-4
54-55	0.0	0.001172738492057134	0.0	0.06287905272844607	2.7508680677883386E-4
56-57	0.0	0.0012016949980338532	0.0	0.06889717988727426	2.799128911082871E-4
58-59	0.0	0.0012113471666927596	0.0	0.07489117662445516	2.799128911082871E-4
60-61	0.0	0.001240303672669479	0.0	0.08019986938685371	2.799128911082871E-4
62-63	0.0	0.0012499558413283855	0.0	0.08548443172760499	2.8956505976719356E-4
64-65	0.0	0.0013706079495647162	0.0	0.09070142888774393	2.8956505976719356E-4
66-67	0.0	0.0014043905398708888	0.0	0.09623694761362678	2.943911440966468E-4
68-69	0.0	0.001409216624200342	0.0	0.10183037935146308	2.992172284261E-4
70-71	0.0	0.0014188687928592485	0.0	0.10661785500628065	2.992172284261E-4
72-73	0.0	0.0014429992145065145	0.0	0.11023259216904113	3.474780717206323E-4
74-75	0.0	0.0014623035518243274	0.0	0.1131041123450658	3.474780717206323E-4
76-77	0.0	0.0014960861421305	0.0	0.11568606746132329	3.474780717206323E-4
78-79	0.0	0.001515390479448313	0.0	0.1175103273378566	3.474780717206323E-4
80-81	0.0	0.0015298687324366727	0.0	0.11814254438501497	3.716084933678984E-4
82-83	0.0	0.0015443469854250322	0.0	0.11856241372167739	3.957389150151645E-4
84-85	0.0	0.0015684774070722984	0.0	0.11874580492619663	3.957389150151645E-4
86-87	0.0	0.001582955660060658	0.0	0.11881337010680898	3.957389150151645E-4
88	0.0	0.001669825177990816	0.0	0.11882784835979733	3.957389150151645E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14690	0.0	48.84222	1
GTATCAA	26940	0.0	38.886345	1
CTTATAC	8635	0.0	35.378857	1
TCAACGC	32520	0.0	31.611956	4
ATCAACG	32805	0.0	31.350252	3
CAACGCA	33315	0.0	30.883064	5
TATCAAC	34030	0.0	30.46037	2
AACGCAG	34450	0.0	29.877033	6
TATACAC	12365	0.0	28.307941	3
ACGCAGA	39055	0.0	26.36355	7
CGCAGAG	39720	0.0	25.879444	8
TTATACA	13375	0.0	24.458748	2
GTGGTAT	6570	0.0	22.474367	1
GCAGAGT	46235	0.0	22.205227	9
TGGTATC	6760	0.0	21.02673	2
GAGTACT	26955	0.0	20.735859	12-13
TACACAT	16555	0.0	20.451359	5
ACACATC	15215	0.0	20.272081	6
GTACATG	33320	0.0	20.209034	1
TACATGG	32760	0.0	20.140032	2
>>END_MODULE