FastQCFastQC Report
Fri 27 May 2016
SRR2140364_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140364_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10510204
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA139650.050.26451
GTATCAA254750.039.63531
CTTATAC86450.034.270931
TCAACGC311950.031.9567744
ATCAACG312850.031.892053
TATCAAC323650.031.0397382
CAACGCA321550.031.002695
AACGCAG328800.030.3046976
TATACAC120600.027.0360013
ACGCAGA380100.026.2349137
CGCAGAG387950.025.7040628
TTATACA134900.023.2876722
GCAGAGT454700.021.9681439
ACACATC141750.021.5296236
TACACAT159050.020.9015435
ACATCTC144800.020.8980738
GTACATG318900.020.783361
TACATGG311800.020.6013092
GTGGTAT65250.019.7671361
GAGTACT275150.019.75270512-13
Produced by FastQC (version 0.11.5)