##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140364_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10510204
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.419177115877105	34.0	31.0	34.0	31.0	34.0
2	32.50791916122655	34.0	31.0	34.0	31.0	34.0
3	32.562067491744216	34.0	31.0	34.0	31.0	34.0
4	36.07734502584346	37.0	35.0	37.0	35.0	37.0
5	36.12330293493827	37.0	35.0	37.0	35.0	37.0
6	36.117210094114256	37.0	36.0	37.0	35.0	37.0
7	36.085189973477206	37.0	36.0	37.0	35.0	37.0
8	36.07337859474469	37.0	35.0	37.0	35.0	37.0
9	37.82195388405401	39.0	38.0	39.0	35.0	39.0
10-11	37.763147699131245	39.0	37.5	39.0	35.0	39.0
12-13	37.75126805340791	39.0	38.0	39.0	35.0	39.0
14-15	39.111753967858284	40.0	38.5	41.0	36.0	41.0
16-17	39.12478654077504	40.0	38.5	41.0	36.0	41.0
18-19	39.167647459554544	40.0	39.0	41.0	36.0	41.0
20-21	39.193975181908456	40.0	39.0	41.0	36.0	41.0
22-23	39.15627424044594	40.0	39.0	41.0	36.0	41.0
24-25	39.12358973353412	40.0	39.0	41.0	36.0	41.0
26-27	39.071447118856135	40.0	39.0	41.0	36.0	41.0
28-29	39.03729897279428	40.0	39.0	41.0	35.5	41.0
30-31	38.95948304640867	40.0	38.0	41.0	35.0	41.0
32-33	38.862229227639695	40.0	38.0	41.0	35.0	41.0
34-35	38.79405444409534	40.0	38.0	41.0	35.0	41.0
36-37	38.73895966571476	40.0	38.0	41.0	35.0	41.0
38-39	38.69409282917344	40.0	38.0	41.0	35.0	41.0
40-41	38.65484227901232	40.0	38.0	41.0	35.0	41.0
42-43	38.59888688752254	40.0	38.0	41.0	35.0	41.0
44-45	38.56205308933556	40.0	38.0	41.0	34.5	41.0
46-47	38.49228566580876	40.0	38.0	41.0	34.0	41.0
48-49	38.427857397677286	40.0	38.0	41.0	34.0	41.0
50-51	38.344472037829696	40.0	38.0	41.0	34.0	41.0
52-53	38.24286454175561	40.0	37.5	41.0	34.0	41.0
54-55	38.13438283433578	40.0	37.0	41.0	33.5	41.0
56-57	38.000813608567555	40.0	37.0	41.0	33.0	41.0
58-59	37.860392546379	40.0	36.5	41.0	33.0	41.0
60-61	37.685102823817445	39.0	36.0	41.0	33.0	41.0
62-63	37.47895783336517	39.0	35.5	41.0	33.0	41.0
64-65	37.253634137744285	39.0	35.0	40.5	33.0	41.0
66-67	36.983144346715974	38.0	35.0	40.0	32.0	41.0
68-69	36.71824942649134	37.5	35.0	40.0	32.0	41.0
70-71	36.41117400105142	37.0	35.0	39.5	32.0	41.0
72-73	36.086037706217056	36.5	35.0	39.0	31.5	41.0
74-75	35.76390917512081	36.0	35.0	39.0	31.0	40.5
76-77	35.43352512991024	36.0	34.0	38.5	31.0	40.0
78-79	35.132754105067036	35.0	34.0	37.0	31.0	39.0
80-81	34.84270479030425	35.0	34.0	37.0	31.0	39.0
82-83	34.56032413933829	35.0	34.0	36.5	31.0	39.0
84-85	34.32293683156591	35.0	34.0	36.0	31.0	37.0
86-87	34.119190499542285	35.0	34.0	36.0	31.0	37.0
88-89	33.97117738575514	35.0	34.0	35.0	31.0	37.0
90-91	33.86926093065328	35.0	34.0	35.0	31.0	36.0
92-93	33.78117486476353	35.0	34.0	35.0	31.0	36.0
94-95	33.68805905902968	35.0	34.0	35.0	31.0	36.0
96-97	33.690226737335465	35.0	34.0	35.0	31.0	36.0
98-99	33.765338593658726	35.0	34.0	35.0	31.0	35.0
100	32.99028050015505	34.0	32.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	0.0
18	3.0
19	27.0
20	86.0
21	321.0
22	1009.0
23	3115.0
24	7927.0
25	16989.0
26	31236.0
27	51315.0
28	76365.0
29	107417.0
30	142393.0
31	186056.0
32	240682.0
33	315776.0
34	444815.0
35	655623.0
36	1116447.0
37	2259710.0
38	3150159.0
39	1650003.0
40	52729.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.95713080355053	20.45070676078219	14.220313896856807	24.37184853881047
2	14.212763706224912	21.10234975273698	42.01047817060478	22.674408370433333
3	17.724365768732937	26.523909526399297	30.63448625735523	25.11723844751253
4	12.273543795550989	18.595954406195887	38.932427546573805	30.19807425167932
5	13.197875600433468	37.8093701827236	35.47469818117531	13.518056035667625
6	29.189734587913613	38.20910783569677	19.079914409226507	13.521243167163105
7	26.047044974794957	33.04023387570815	23.10745240004042	17.80526874945648
8	24.122633585418512	36.738497178551434	21.388557253503357	17.750311982526696
9	25.373551265037293	17.16006654104906	21.355522690139985	36.11085950377367
10-11	23.922932418818892	27.00037030679899	28.9772729435128	20.099424330869315
12-13	24.953411941385724	24.42896446158419	29.007700516564665	21.60992308046542
14-15	22.117410851397366	25.785617481830037	26.76437108166502	25.332600585107578
16-17	21.59735909978531	28.906865175975653	27.380753028200022	22.11502269603901
18-19	21.57968104139558	27.861452546496718	29.27872760604837	21.28013880605933
20-21	22.41712358716452	27.001704748647697	29.181778214641525	21.39939344954626
22-23	22.08261635507404	27.091450859481775	29.030231443317977	21.795701342126208
24-25	21.880134416681717	27.25339867120624	29.173788604281086	21.692678307830953
26-27	21.894722911371105	27.42537019454032	29.092864791964846	21.587042102123732
28-29	21.753498034413425	27.259107582970334	29.21415355700082	21.773240825615424
30-31	22.108623833505938	27.373275858245734	28.911322440611087	21.606777867637245
32-33	22.157806178701755	27.242972625794344	28.90878661471833	21.690434580785574
34-35	22.041275582969902	27.284372427538216	28.939611150313954	21.73474083917793
36-37	21.94351735969043	27.388830006713704	28.887658248486286	21.77999438510958
38-39	21.79701468897871	27.314997547270586	29.020479491900186	21.867508271850518
40-41	22.133438715904727	27.158943984391794	28.904187724544755	21.803429575158724
42-43	22.170219839375697	27.187737280769497	28.678422284163673	21.963620595691133
44-45	22.069789339999033	27.508574937271526	28.44454854103341	21.97708718169603
46-47	22.354159141976936	27.309322904384114	27.99822376502035	22.3382941886186
48-49	22.204146359491926	27.44068169038104	28.008247410196645	22.346924539930388
50-51	22.118127081011913	27.587919772738427	28.01708989106695	22.276863255182707
52-53	22.312776699494783	27.513206582458622	28.058287645158902	22.11572907288769
54-55	22.261678338819134	27.415208383258634	27.945460250443375	22.37765302747885
56-57	22.41838575665988	27.674678837771605	27.689765049042155	22.21717035652636
58-59	22.162029633103337	27.884742163290944	27.963248685187587	21.989979518418128
60-61	22.375934603580685	28.02951813835786	27.633495883360986	21.961051374700467
62-63	22.18363179817038	28.219019537689128	27.56815087439789	22.029197789742604
64-65	22.16050191909101	28.126699828593775	27.563354972750005	22.149443279565205
66-67	22.241488261787485	28.081988065406865	27.472169991150096	22.20435368165555
68-69	22.27176233985159	27.921514611493635	27.5875445195041	22.219178529150675
70-71	22.30380120567512	27.521199174770906	27.719697584839743	22.45530203471423
72-73	22.35320043737909	27.57074046036953	27.669251689236553	22.406807413014832
74-75	22.20149645236951	27.73115736506145	27.622811828832493	22.444534353736554
76-77	22.3048786745687	27.69125988498787	27.584732075681494	22.41912936476194
78-79	22.315647099255546	27.69459159871174	27.535031591267717	22.454729710764994
80-81	22.328448795837403	27.679838188695133	27.50819544056922	22.483517574898244
82-83	22.360479516820593	27.670580151557537	27.6339072192455	22.33503311237637
84-85	22.490090889296283	27.604103126737666	27.586860852411004	22.318945131555047
86-87	22.26436978327918	27.6982326908624	27.666578013567282	22.37081951229114
88-89	22.35120773884786	27.69557045123055	27.579686737806732	22.37353507211486
90-91	22.391140152437494	27.69242037835668	27.64391106507198	22.272528404133844
92-93	22.40402525252651	27.64748361403717	27.634606397396254	22.313884736040066
94-95	22.31114515068788	27.734865669281	27.65576653912547	22.298222640905653
96-97	22.34029712928068	27.852601682675726	27.75750981286332	22.049591375180274
98-99	23.40418563018234	29.77226042452994	27.89559257271993	18.927961372567793
100	22.4461763700333	30.795378114420856	21.520728283569486	25.237717231976358
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1277.0
1	1198.0
2	2347.5
3	3697.0
4	4861.5
5	8597.0
6	11811.5
7	13627.5
8	14475.5
9	13320.0
10	10889.5
11	8461.0
12	8732.0
13	12582.5
14	23193.0
15	38733.0
16	52344.5
17	61262.0
18	63865.5
19	60480.0
20	53764.0
21	46388.0
22	41928.0
23	43568.5
24	50953.5
25	64724.0
26	84832.5
27	106703.0
28	127369.0
29	150334.5
30	173045.5
31	195580.5
32	222766.0
33	249819.5
34	275377.0
35	299264.5
36	324952.5
37	347707.0
38	361805.0
39	375283.5
40	391696.0
41	404019.0
42	411612.0
43	420890.0
44	440420.5
45	459748.0
46	456889.0
47	448086.0
48	441834.5
49	432340.5
50	423003.0
51	408961.5
52	388310.5
53	364787.0
54	341615.5
55	317447.0
56	292349.0
57	265337.0
58	236246.0
59	206580.0
60	173719.0
61	140759.0
62	111456.0
63	86279.0
64	65184.5
65	48404.5
66	36298.5
67	28076.0
68	21695.5
69	16151.5
70	11939.5
71	8712.0
72	6287.5
73	4406.0
74	3043.5
75	2110.5
76	1423.5
77	938.0
78	659.5
79	473.0
80	331.5
81	219.5
82	150.0
83	100.0
84	67.5
85	50.5
86	42.0
87	35.0
88	25.0
89	15.0
90	8.5
91	6.0
92	5.5
93	4.0
94	3.0
95	2.5
96	2.0
97	2.0
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.005185436933479122
3	0.0
4	3.805825272278254E-5
5	0.004804854406251296
6	0.00627961169925912
7	5.708737908417382E-5
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.010840875506821638
26-27	0.0022754685025413125
28-29	0.0
30-31	4.291871895635409E-4
32-33	0.0028389921520506844
34-35	6.471183245790207E-4
36-37	0.002988807421560169
38-39	6.776828204307042E-5
40-41	4.384983633292199E-5
42-43	4.9062963629674125E-6
44-45	0.0
46-47	0.003068929557370389
48-49	0.0
50-51	0.0015463884225561302
52-53	5.124242380764004E-6
54-55	4.5945692707463517E-4
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0016056611451127196
64-65	0.001054730163431515
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0026914911870485445
74-75	2.205934007730724E-4
76-77	0.0030650849382411072
78-79	0.0
80-81	0.0
82-83	7.586228959682191E-4
84-85	4.6327632342009115E-4
86-87	0.0015542054970508201
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0014568356621261324
96-97	8.749919577945055E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	269.0
20-21	974.0
22-23	1893.0
24-25	2764.0
26-27	4891.0
28-29	9309.0
30-31	22151.0
32-33	28631.0
34-35	37766.0
36-37	55539.0
38-39	66885.0
40-41	69203.0
42-43	81900.0
44-45	99495.0
46-47	97051.0
48-49	83389.0
50-51	73114.0
52-53	70894.0
54-55	76634.0
56-57	78153.0
58-59	78919.0
60-61	77797.0
62-63	79470.0
64-65	90599.0
66-67	96643.0
68-69	97557.0
70-71	91715.0
72-73	77550.0
74-75	78496.0
76-77	83739.0
78-79	86025.0
80-81	87546.0
82-83	83926.0
84-85	85069.0
86-87	89745.0
88-89	95685.0
90-91	100951.0
92-93	109082.0
94-95	132733.0
96-97	352313.0
98-99	676167.0
100-101	6797572.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.26452938250381
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.28634929751897	72.62834975859744
2	7.789942140909453	12.816718482777006
3	2.114296124623094	5.217947270021113
4	0.7909534960792001	2.602696684736059
5	0.3810552725497339	1.567366633251279
6	0.20486895735627778	1.011206901719906
7	0.12803742002062646	0.7373056670942765
8	0.07879313714929415	0.5185504276926209
9	0.051581777596845846	0.3819015592845752
>10	0.16952416161184256	2.1054340445186273
>50	0.003541458107979721	0.19610892185544623
>100	9.523828959804295E-4	0.129865450365925
>500	6.262533392643534E-5	0.034156428007904194
>1k	4.174824678115747E-5	0.05239177007781647
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	9.514563180695637E-6	0.0	9.514563180695637E-6	0.0
3	0.0	9.514563180695637E-6	0.0	9.514563180695637E-6	0.0
4	0.0	2.854368954208691E-5	0.0	9.514563180695637E-6	0.0
5	0.0	2.854368954208691E-5	0.0	9.514563180695637E-6	0.0
6	0.0	2.854368954208691E-5	0.0	9.514563180695637E-6	0.0
7	0.0	4.757281590347818E-5	0.0	1.9029126361391274E-5	0.0
8	0.0	5.708737908417382E-5	0.0	1.9029126361391274E-5	0.0
9	0.0	9.514563180695636E-5	0.0	1.9029126361391274E-5	9.514563180695637E-6
10-11	0.0	9.514563180695636E-5	0.0	9.514563180695637E-5	9.514563180695637E-6
12-13	0.0	9.514563180695636E-5	0.0	1.0941747657799983E-4	9.514563180695637E-6
14-15	0.0	1.7126213725252146E-4	0.0	1.284466029393911E-4	1.9029126361391274E-5
16-17	0.0	1.7126213725252146E-4	0.0	1.332038845297389E-4	1.9029126361391274E-5
18-19	0.0	1.9504854520426056E-4	0.0	1.332038845297389E-4	1.9029126361391274E-5
20-21	0.0	2.2834951633669527E-4	0.0	1.4271844771043455E-4	1.9029126361391274E-5
22-23	0.0	2.568932058787822E-4	0.0	1.6174757407182584E-4	2.3786407951739093E-5
24-25	0.0	2.806796138305213E-4	0.0	1.9029126361391275E-4	2.854368954208691E-5
26-27	0.0	2.949514586015647E-4	0.0	2.473786426980865E-4	2.854368954208691E-5
28-29	0.0	2.949514586015647E-4	0.0	4.900000038058253E-4	2.854368954208691E-5
30-31	0.0	3.8058252722782544E-4	0.0	0.0011227184553220851	2.854368954208691E-5
32-33	0.0	3.8058252722782544E-4	0.0	0.0028163107014859082	2.854368954208691E-5
34-35	0.0	4.2815534313130364E-4	0.0	0.0047953398430706	3.805825272278255E-5
36-37	0.0	4.8048544062512965E-4	0.0	0.008077864140410595	3.805825272278255E-5
38-39	0.0	4.8524272221547746E-4	0.0	0.013134854470950325	3.805825272278255E-5
40-41	0.0	5.423301012996512E-4	0.0	0.018115728296044492	3.805825272278255E-5
42-43	0.0	5.470873828899991E-4	0.0	0.02333446620065605	3.805825272278255E-5
44-45	0.0	5.899029172031294E-4	0.0	0.029162136148832125	3.805825272278255E-5
46-47	0.0	6.184466067452164E-4	0.0	0.03503262163132133	4.2815534313130364E-5
48-49	0.0	6.184466067452164E-4	0.0	0.0412075731355928	4.757281590347818E-5
50-51	0.0	6.755339858293902E-4	0.0	0.04793436930434461	4.757281590347818E-5
52-53	0.0	6.850485490100858E-4	0.0	0.053861942165918	5.2330097493825996E-5
54-55	0.0	6.850485490100858E-4	0.0	0.05938038881072147	5.708737908417382E-5
56-57	0.0	7.135922385521727E-4	0.0	0.0632052432093611	5.708737908417382E-5
58-59	0.0	7.135922385521727E-4	0.0	0.06722038887161466	6.184466067452163E-5
60-61	9.514563180695637E-6	7.32621364913564E-4	0.0	0.07106902967820605	6.660194226486945E-5
62-63	9.514563180695637E-6	7.32621364913564E-4	0.0	0.07495097145592988	6.660194226486945E-5
64-65	9.514563180695637E-6	7.897087439977378E-4	0.0	0.07867592294117222	7.135922385521727E-5
66-67	9.514563180695637E-6	7.897087439977378E-4	0.0	0.08260068025320917	7.61165054455651E-5
68-69	9.514563180695637E-6	8.372815599012159E-4	0.0	0.0864445637782102	7.61165054455651E-5
70-71	9.514563180695637E-6	8.658252494433029E-4	0.0	0.0899554375918869	7.61165054455651E-5
72-73	9.514563180695637E-6	8.658252494433029E-4	0.0	0.09259097159293958	1.1417475816834764E-4
74-75	9.514563180695637E-6	8.991262205757377E-4	0.0	0.09480310753245132	1.1417475816834764E-4
76-77	9.514563180695637E-6	9.324271917081723E-4	0.0	0.09699145706401131	1.1417475816834764E-4
78-79	9.514563180695637E-6	9.324271917081723E-4	0.0	0.09841864154111565	1.1417475816834764E-4
80-81	9.514563180695637E-6	9.324271917081723E-4	0.0	0.09891339882651183	1.1417475816834764E-4
82-83	9.514563180695637E-6	9.41941754888868E-4	0.0	0.09916553475080028	1.1417475816834764E-4
84-85	9.514563180695637E-6	9.609708812502593E-4	0.0	0.09925592310101689	1.1417475816834764E-4
86-87	9.514563180695637E-6	9.704854444309549E-4	0.0	0.09929873863533001	1.2368932134904328E-4
88	9.514563180695637E-6	9.895145707923463E-4	0.0	0.09931301048010105	1.332038845297389E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13965	0.0	50.2645	1
GTATCAA	25475	0.0	39.6353	1
CTTATAC	8645	0.0	34.27093	1
TCAACGC	31195	0.0	31.956774	4
ATCAACG	31285	0.0	31.89205	3
TATCAAC	32365	0.0	31.039738	2
CAACGCA	32155	0.0	31.00269	5
AACGCAG	32880	0.0	30.304697	6
TATACAC	12060	0.0	27.036001	3
ACGCAGA	38010	0.0	26.234913	7
CGCAGAG	38795	0.0	25.704062	8
TTATACA	13490	0.0	23.287672	2
GCAGAGT	45470	0.0	21.968143	9
ACACATC	14175	0.0	21.529623	6
TACACAT	15905	0.0	20.901543	5
ACATCTC	14480	0.0	20.898073	8
GTACATG	31890	0.0	20.78336	1
TACATGG	31180	0.0	20.601309	2
GTGGTAT	6525	0.0	19.767136	1
GAGTACT	27515	0.0	19.752705	12-13
>>END_MODULE