FastQCFastQC Report
Fri 27 May 2016
SRR2140363_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140363_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8086609
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA118050.054.841631
GTATCAA218350.037.548751
TCAACGC269500.029.9633644
ATCAACG272650.029.617193
CAACGCA277600.029.1196755
TATCAAC279500.029.0297242
AACGCAG285950.028.268486
GTGGTAT51200.026.550421
TGGTATC48400.025.4532972
ACGCAGA327500.024.6937987
CGCAGAG333150.024.3005088
GTACATG286450.021.4887391
TACATGG281400.021.1950472
GCAGAGT386550.020.888579
GAGTACT223850.020.36935412-13
ACATGGG288300.020.3035413
CAGAGTA376950.019.25708810-11
AGAGTAC351900.018.46734610-11
GTACTTT248150.018.27323514-15
CATGGGG211150.018.1058374
Produced by FastQC (version 0.11.5)