##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140363_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8086609
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.244140900097925	33.0	31.0	34.0	30.0	34.0
2	32.23732914500998	33.0	31.0	34.0	30.0	34.0
3	32.312432813309016	34.0	31.0	34.0	30.0	34.0
4	35.98430442228628	37.0	35.0	37.0	35.0	37.0
5	36.0434043490912	37.0	35.0	37.0	35.0	37.0
6	36.066017288581655	37.0	35.0	37.0	35.0	37.0
7	36.033107202289614	37.0	35.0	37.0	35.0	37.0
8	36.01592855546744	37.0	35.0	37.0	35.0	37.0
9	37.77158621122896	39.0	38.0	39.0	35.0	39.0
10-11	37.71078210903977	39.0	37.0	39.0	35.0	39.0
12-13	37.70446741025812	39.0	37.0	39.0	35.0	39.0
14-15	39.02784356211609	40.0	38.0	41.0	36.0	41.0
16-17	39.05158484848222	40.0	38.0	41.0	36.0	41.0
18-19	39.10960818063542	40.0	38.5	41.0	36.0	41.0
20-21	39.14471150625779	40.0	39.0	41.0	36.0	41.0
22-23	39.10978541438281	40.0	39.0	41.0	36.0	41.0
24-25	39.078343981034855	40.0	39.0	41.0	36.0	41.0
26-27	39.02602540878345	40.0	39.0	41.0	35.5	41.0
28-29	38.99031336910495	40.0	38.0	41.0	35.0	41.0
30-31	38.91593981577286	40.0	38.0	41.0	35.0	41.0
32-33	38.81978240041056	40.0	38.0	41.0	35.0	41.0
34-35	38.75186665645713	40.0	38.0	41.0	35.0	41.0
36-37	38.70633049170292	40.0	38.0	41.0	35.0	41.0
38-39	38.669623947629645	40.0	38.0	41.0	35.0	41.0
40-41	38.61500209513546	40.0	38.0	41.0	35.0	41.0
42-43	38.548735284522536	40.0	38.0	41.0	34.0	41.0
44-45	38.50565277894667	40.0	38.0	41.0	34.0	41.0
46-47	38.43184708917116	40.0	38.0	41.0	34.0	41.0
48-49	38.362402796309425	40.0	38.0	41.0	34.0	41.0
50-51	38.288995207720056	40.0	38.0	41.0	34.0	41.0
52-53	38.180612320306345	40.0	37.0	41.0	33.5	41.0
54-55	38.06479258372562	40.0	37.0	41.0	33.0	41.0
56-57	37.923218079745304	40.0	37.0	41.0	33.0	41.0
58-59	37.77404642779146	39.5	36.0	41.0	33.0	41.0
60-61	37.59831173138758	39.0	36.0	41.0	33.0	41.0
62-63	37.38359649320881	39.0	35.5	41.0	33.0	41.0
64-65	37.15151002234656	39.0	35.0	40.0	32.0	41.0
66-67	36.86988000135943	38.0	35.0	40.0	32.0	41.0
68-69	36.59496923207092	37.0	35.0	40.0	32.0	41.0
70-71	36.28400587409753	37.0	35.0	39.5	31.5	41.0
72-73	35.95857284763136	36.5	35.0	39.0	31.0	41.0
74-75	35.63679026736315	36.0	34.5	39.0	31.0	40.0
76-77	35.309619648392726	35.5	34.0	37.5	31.0	40.0
78-79	35.01497840461179	35.0	34.0	37.0	31.0	39.0
80-81	34.732997585558124	35.0	34.0	37.0	31.0	39.0
82-83	34.45500752583088	35.0	34.0	36.0	31.0	38.5
84-85	34.22933701810818	35.0	34.0	36.0	31.0	37.0
86-87	34.03616017846721	35.0	34.0	35.5	30.0	37.0
88-89	33.897007160225684	35.0	34.0	35.0	30.0	36.5
90-91	33.80403635466445	35.0	34.0	35.0	30.0	36.0
92-93	33.723084191669415	35.0	34.0	35.0	30.0	36.0
94-95	33.6348603776891	35.0	34.0	35.0	30.0	36.0
96-97	33.640009306894996	35.0	34.0	35.0	30.5	35.5
98-99	33.72355124721645	35.0	34.0	35.0	31.0	35.0
100	32.94062413898529	34.0	32.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	3.0
19	19.0
20	63.0
21	231.0
22	835.0
23	2610.0
24	6254.0
25	13461.0
26	25288.0
27	41233.0
28	61968.0
29	86554.0
30	114074.0
31	148158.0
32	192029.0
33	252401.0
34	356174.0
35	525525.0
36	894088.0
37	1761617.0
38	2371862.0
39	1197472.0
40	34689.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.54371752115132	20.070266783023637	14.300221514357872	24.08579418146716
2	14.417532303390935	20.9705288599714	41.68742644829157	22.924512388346095
3	18.007795356496153	26.35855152635672	30.27043844954047	25.36321466760666
4	12.463411720566082	18.210223176447574	38.7094412662123	30.616923836774045
5	13.406786162929349	37.82302562439728	35.032569990791806	13.737618221881561
6	29.719588051117913	37.82537657152651	18.823073154094	13.631962223261574
7	26.518151986593143	32.90272015163836	22.908356631111587	17.67077123065691
8	24.58041436156985	36.538183062888294	20.97336473174355	17.908037843798308
9	25.813341537843616	17.05350165934819	21.19945455505515	35.93370224775305
10-11	24.291962180933936	26.911811860818297	28.514498475195225	20.28172748305254
12-13	25.225165455631647	24.480527004582513	28.6864432297889	21.607864309996934
14-15	22.41909433237096	25.692975881485058	26.598819109468508	25.289110676675474
16-17	21.69329566942089	28.767027069071844	27.276216767745293	22.263460493761972
18-19	21.73011580008382	27.743674759098653	29.221760320055047	21.304449120762488
20-21	22.69704403951504	26.815937151491216	29.165799967797142	21.32121884119661
22-23	22.35028550176439	26.789474003180363	28.984993517198443	21.875246977856808
24-25	22.08312056097053	26.94973355384334	29.105960172725283	21.861185712460845
26-27	21.982151349340615	27.117038386713833	29.035699652528347	21.8651106114172
28-29	21.92289527381967	27.041280849700033	29.09579895150687	21.94002492497343
30-31	22.2908133540113	26.94563033772074	28.957132481440688	21.806423826827274
32-33	22.058277329386478	27.11552554372179	28.93382972293807	21.892367403953664
34-35	22.194186178722315	27.17545166302956	28.873919339974545	21.756442818273577
36-37	22.225039663309527	26.994028277090948	28.820493941249463	21.960438118350055
38-39	22.006590757027396	27.191991154871705	28.879393026946055	21.922025061154844
40-41	22.291800166426263	27.02300944285392	28.766412421588416	21.918777969131398
42-43	22.217662917384118	27.068733437899073	28.549616954478115	22.163986690238698
44-45	22.23293974960846	27.256914546627954	28.340027649285698	22.170118054477893
46-47	22.407801150318345	27.225534877172493	27.960883440317456	22.405780532191706
48-49	22.30420560467749	27.37665997589056	27.90953477394456	22.409599645487386
50-51	22.30912226983202	27.532329145825397	27.767453224150955	22.39109536019163
52-53	22.473058672536347	27.45446312258778	27.692312013606042	22.380166191269833
54-55	22.451054090063437	27.358604820968367	27.731500379227235	22.458840709740954
56-57	22.3930142458505	27.523988650783366	27.65357722504906	22.429419878317074
58-59	22.337662355441438	27.537560995376648	27.763759313927615	22.361017335254303
60-61	22.44637947603381	27.604051936727096	27.65595028196513	22.293618305273963
62-63	22.381854420381828	27.653765613564218	27.65299996554584	22.311380000508112
64-65	22.34202350174746	27.63008512273749	27.561612988986905	22.466278386528142
66-67	22.45281483667283	27.698037332025816	27.398308713678727	22.45083911762263
68-69	22.393844582630575	27.594747389377154	27.50825795409741	22.503150073894858
70-71	22.406997279975855	27.293860687044802	27.667031342885352	22.63211069009399
72-73	22.463475679768045	27.36556220632786	27.49196936541873	22.678992748485367
74-75	22.34062327848131	27.531272587757872	27.47800607194198	22.65009806181884
76-77	22.507298979981634	27.47199190676287	27.401871575915322	22.61883753734017
78-79	22.483321759851908	27.469328777004915	27.35792840371897	22.689421059424213
80-81	22.504615359022566	27.512159859476824	27.294289960615853	22.68893482088475
82-83	22.525923386489378	27.508481225806392	27.435799190727746	22.52979619697649
84-85	22.575091945857636	27.4909165084089	27.393145921578622	22.540845624154834
86-87	22.409099543384407	27.526669036745393	27.491515380581976	22.572716039288224
88-89	22.532728228810015	27.542854675229915	27.399923672417398	22.52449342354267
90-91	22.55509097667826	27.571697647126552	27.43813144139393	22.435079934801255
92-93	22.5934471165147	27.523367078220733	27.45277711586791	22.430408689396657
94-95	22.433141359905743	27.613778349115886	27.49413861974887	22.4589416712295
96-97	22.500699438143094	27.694750486932623	27.57094909995598	22.233600974968304
98-99	23.591035085592235	29.612057981578378	27.717832036085298	19.079074896744093
100	22.685721529607626	30.53790797398328	21.423240939185522	25.353129557223575
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1512.0
1	1344.0
2	2146.5
3	3192.0
4	3974.5
5	6658.5
6	9117.5
7	10785.5
8	11886.0
9	11502.5
10	9801.0
11	7698.0
12	7713.5
13	10441.5
14	18598.0
15	30707.0
16	41148.5
17	47944.5
18	49871.5
19	46885.0
20	41084.5
21	34925.5
22	31000.5
23	31696.5
24	36887.5
25	46179.0
26	59454.0
27	74854.5
28	90117.5
29	107188.0
30	125570.0
31	144020.0
32	164638.5
33	184678.5
34	204226.5
35	223653.0
36	245243.5
37	265527.0
38	277679.5
39	287383.5
40	299458.0
41	307887.0
42	312533.5
43	320724.5
44	328398.5
45	333834.0
46	337540.5
47	338338.5
48	338085.0
49	334298.0
50	326582.0
51	317046.0
52	305005.0
53	290135.5
54	274206.5
55	257011.0
56	238445.0
57	217964.5
58	196052.0
59	173188.5
60	147492.5
61	121345.0
62	96568.0
63	74599.5
64	56698.0
65	42401.5
66	31573.0
67	24186.0
68	18581.5
69	13566.5
70	9807.5
71	7186.5
72	5065.5
73	3474.0
74	2383.5
75	1576.5
76	1046.5
77	684.5
78	481.5
79	365.0
80	276.0
81	212.0
82	166.5
83	121.5
84	79.0
85	49.5
86	33.0
87	21.5
88	14.0
89	10.5
90	8.5
91	6.0
92	3.5
93	3.0
94	2.0
95	1.0
96	1.0
97	0.5
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.005700782614814195
3	0.0
4	3.709836842612274E-5
5	0.0040931866496822095
6	0.004488902579560852
7	1.236612280870758E-5
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.011046961896826447
26-27	0.002338780092303854
28-29	0.0
30-31	3.9051521602247977E-4
32-33	0.003069665054181142
34-35	6.233579194008183E-4
36-37	0.0013147885538265043
38-39	8.189460265431747E-5
40-41	5.709520168118724E-5
42-43	6.391548429944891E-6
44-45	0.0
46-47	0.0020987915523248937
48-49	0.0
50-51	6.107943832410768E-4
52-53	6.687723186042027E-6
54-55	7.409407698010863E-5
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0010510148697026936
64-65	5.193636307616553E-4
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0026874272974444532
74-75	3.9674212995861543E-4
76-77	0.003039913470716662
78-79	0.0
80-81	0.0
82-83	9.548258157096039E-4
84-85	4.011717919449024E-4
86-87	0.0018005207012334423
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0013268555481031413
96-97	9.02492684795405E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	223.0
20-21	798.0
22-23	1500.0
24-25	2197.0
26-27	3793.0
28-29	7342.0
30-31	18883.0
32-33	23657.0
34-35	31461.0
36-37	46476.0
38-39	54413.0
40-41	57467.0
42-43	66979.0
44-45	80112.0
46-47	78116.0
48-49	67033.0
50-51	56925.0
52-53	52457.0
54-55	56239.0
56-57	60389.0
58-59	61852.0
60-61	60078.0
62-63	58994.0
64-65	62388.0
66-67	65275.0
68-69	66507.0
70-71	64726.0
72-73	59927.0
74-75	60988.0
76-77	64016.0
78-79	65994.0
80-81	67212.0
82-83	65024.0
84-85	65505.0
86-87	68799.0
88-89	73106.0
90-91	77244.0
92-93	83888.0
94-95	102935.0
96-97	275503.0
98-99	520167.0
100-101	5230021.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.52306302195825
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.57332105629547	78.36681165861911
2	6.729153602160724	11.644539624083794
3	1.5736108777459175	4.084608994417471
4	0.5498415214735786	1.902958904581914
5	0.22691493221876374	0.9816687490493741
6	0.11669868938877202	0.6058276833938792
7	0.06731513101689161	0.4077017926314123
8	0.039841326738568016	0.2757754899423641
9	0.029422513871507648	0.22911534197720074
>10	0.0909836659968806	1.2066861802134674
>50	0.002275246410336457	0.13686700210908825
>100	5.559465645373233E-4	0.09554271124356202
>500	2.1830289848876406E-5	0.010785182438420927
>1k	4.365982814801835E-5	0.05111068529896133
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.2366122808707581E-5	0.0	0.0	1.2366122808707581E-5
3	0.0	1.2366122808707581E-5	0.0	0.0	1.2366122808707581E-5
4	0.0	7.419673685224549E-5	0.0	1.2366122808707581E-5	1.2366122808707581E-5
5	0.0	7.419673685224549E-5	0.0	2.4732245617415163E-5	1.2366122808707581E-5
6	2.4732245617415163E-5	7.419673685224549E-5	0.0	2.4732245617415163E-5	3.709836842612274E-5
7	2.4732245617415163E-5	7.419673685224549E-5	0.0	2.4732245617415163E-5	3.709836842612274E-5
8	3.709836842612274E-5	7.419673685224549E-5	0.0	2.4732245617415163E-5	3.709836842612274E-5
9	3.709836842612274E-5	1.4839347370449097E-4	0.0	3.709836842612274E-5	3.709836842612274E-5
10-11	3.709836842612274E-5	1.4839347370449097E-4	0.0	8.037979825659927E-5	3.709836842612274E-5
12-13	3.709836842612274E-5	1.4839347370449097E-4	0.0	9.892898246966065E-5	3.709836842612274E-5
14-15	3.709836842612274E-5	2.1640714915238267E-4	0.0	1.0511204387401444E-4	4.328142983047653E-5
16-17	4.328142983047653E-5	2.2259021055673646E-4	0.0	1.360273508957834E-4	4.9464491234830326E-5
18-19	4.9464491234830326E-5	2.3495633336544406E-4	0.0	1.854918421306137E-4	5.564755263918412E-5
20-21	4.9464491234830326E-5	2.4732245617415164E-4	0.0	2.3495633336544406E-4	6.80136754478917E-5
22-23	8.656285966095306E-5	2.906038860046281E-4	0.0	2.596885789828592E-4	7.419673685224549E-5
24-25	9.892898246966065E-5	3.215191930263971E-4	0.0	2.8442082460027437E-4	7.419673685224549E-5
26-27	9.892898246966065E-5	3.3388531583510467E-4	0.0	3.709836842612274E-4	7.419673685224549E-5
28-29	9.892898246966065E-5	3.3388531583510467E-4	0.0	6.739536930745632E-4	8.037979825659927E-5
30-31	1.1129510527836823E-4	4.3281429830476534E-4	0.0	0.0020836916932672274	8.656285966095306E-5
32-33	1.1129510527836823E-4	4.451804211134729E-4	0.0	0.005805894658688209	1.1129510527836823E-4
34-35	1.360273508957834E-4	4.946449123483033E-4	0.0	0.009874349062753003	1.2366122808707582E-4
36-37	1.6075959651319855E-4	5.441094035831336E-4	0.0	0.017009601923377277	1.360273508957834E-4
38-39	1.6075959651319855E-4	5.688416492005487E-4	0.0	0.037815603549027785	1.360273508957834E-4
40-41	1.7312571932190612E-4	6.18306140435379E-4	0.0	0.06107009749080239	1.360273508957834E-4
42-43	1.7312571932190612E-4	6.430383860527942E-4	0.0	0.07360934601883187	1.360273508957834E-4
44-45	1.793087807262599E-4	7.172351229050398E-4	0.0	0.08853525624894193	1.4221041230013718E-4
46-47	1.854918421306137E-4	7.543334913311624E-4	0.0	0.10403000812825253	1.6075959651319855E-4
48-49	1.9167490353496752E-4	7.543334913311624E-4	0.0	0.11863439916533618	1.7312571932190612E-4
50-51	2.040410263436751E-4	8.965439036312996E-4	0.0	0.1322927818075537	1.7312571932190612E-4
52-53	2.1022408774802888E-4	9.027269650356533E-4	0.0	0.1770519633136708	2.040410263436751E-4
54-55	2.2877327196109024E-4	9.15093087844361E-4	0.0	0.22975019566297816	2.4113939476979782E-4
56-57	2.4732245617415164E-4	9.274592106530685E-4	0.0	0.27833050911698587	2.4732245617415164E-4
58-59	2.4732245617415164E-4	9.336422720574223E-4	0.0	0.30034220771648534	2.5350551757850545E-4
60-61	2.720547017915668E-4	9.398253334617761E-4	0.0	0.3140315056657247	2.596885789828592E-4
62-63	3.091530702176895E-4	9.645575790791913E-4	0.0	0.3253217757900747	2.9678694740898194E-4
64-65	3.091530702176895E-4	0.0011005849299749746	0.0	0.33147392188740665	2.9678694740898194E-4
66-67	3.091530702176895E-4	0.0011253171755923898	0.0	0.33684700224779013	3.0297000881333576E-4
68-69	3.091530702176895E-4	0.0011871477896359278	0.0	0.34212733668710826	3.215191930263971E-4
70-71	3.091530702176895E-4	0.0011995139124446352	0.0	0.34691302621407816	3.4625143864381225E-4
72-73	3.153361316220433E-4	0.0012180630966576967	0.0	0.3504188220303467	3.709836842612274E-4
74-75	3.215191930263971E-4	0.0012551614650838194	0.0	0.3534547051798844	3.709836842612274E-4
76-77	3.215191930263971E-4	0.001323175140531711	0.0	0.3559897603556695	3.709836842612274E-4
78-79	3.215191930263971E-4	0.0013417243247447726	0.0	0.35775193285591034	3.709836842612274E-4
80-81	3.4625143864381225E-4	0.0013479073861491263	0.0	0.3583393236893239	3.8334980706993503E-4
82-83	3.4625143864381225E-4	0.0013479073861491263	0.0	0.35866084288235034	4.080820526873502E-4
84-85	3.5243450004816606E-4	0.0013726396317665414	0.0	0.3588216024788635	4.080820526873502E-4
86-87	3.586175614525198E-4	0.0014035549387883105	0.0	0.358864883908694	4.2044817549605776E-4
88	3.586175614525198E-4	0.0014839347370449096	0.0	0.358864883908694	4.2044817549605776E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11805	0.0	54.84163	1
GTATCAA	21835	0.0	37.54875	1
TCAACGC	26950	0.0	29.963364	4
ATCAACG	27265	0.0	29.61719	3
CAACGCA	27760	0.0	29.119675	5
TATCAAC	27950	0.0	29.029724	2
AACGCAG	28595	0.0	28.26848	6
GTGGTAT	5120	0.0	26.55042	1
TGGTATC	4840	0.0	25.453297	2
ACGCAGA	32750	0.0	24.693798	7
CGCAGAG	33315	0.0	24.300508	8
GTACATG	28645	0.0	21.488739	1
TACATGG	28140	0.0	21.195047	2
GCAGAGT	38655	0.0	20.88857	9
GAGTACT	22385	0.0	20.369354	12-13
ACATGGG	28830	0.0	20.303541	3
CAGAGTA	37695	0.0	19.257088	10-11
AGAGTAC	35190	0.0	18.467346	10-11
GTACTTT	24815	0.0	18.273235	14-15
CATGGGG	21115	0.0	18.105837	4
>>END_MODULE