FastQCFastQC Report
Fri 27 May 2016
SRR2140362_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140362_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9388307
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA135250.053.3493841
CTTATAC110400.042.254241
GTATCAA260100.040.430911
TATACAC148800.033.455043
TTATACA147750.032.3469732
TCAACGC324300.031.9548024
ATCAACG327700.031.6620463
TATCAAC330950.031.5321872
CAACGCA333600.031.0512735
AACGCAG343500.030.180216
TACACAT182400.027.0832215
ACGCAGA393450.026.3364077
CGCAGAG403350.025.679498
ACACATC184900.025.5706836
GTGGTAT59100.025.306921
TGGTATC58200.023.5870742
CACATCT206500.022.7920637
GCAGAGT460700.022.409239
ATACACA235000.022.3552974
GTACATG336000.021.4116651
Produced by FastQC (version 0.11.5)