FastQCFastQC Report
Fri 27 May 2016
SRR2140361_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140361_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9098118
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC137900.059.0209771
GGTATCA133850.053.7906461
TATACAC179900.047.4717143
TTATACA178400.046.6180652
GTATCAA248900.038.6143761
TACACAT254100.033.076765
ACACATC261450.031.3856376
TCAACGC308100.030.8764634
ATCAACG312450.030.4330563
ACATCTC272050.030.301018
CAACGCA316400.030.0531225
TATCAAC318550.029.9722632
ATACACA299050.029.675054
AACGCAG326400.029.1574956
CACATCT286500.028.6252677
CATCTCC302700.027.0651979
ACGCAGA375300.025.3796837
GTGGTAT58800.024.9642051
CGCAGAG383350.024.857638
TGGTATC56500.024.1835292
Produced by FastQC (version 0.11.5)