FastQCFastQC Report
Fri 27 May 2016
SRR2140360_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140360_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9330254
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA134100.053.337251
CTTATAC85150.041.6265681
GTATCAA259800.040.196081
TCAACGC316650.032.510844
ATCAACG322400.031.9441473
TATCAAC326100.031.7380472
CAACGCA325450.031.6577915
TATACAC124750.030.7885683
AACGCAG335900.030.7233316
TTATACA123700.029.7837452
ACGCAGA386500.026.7111057
CGCAGAG393100.026.2626368
GTGGTAT62850.025.7387731
TGGTATC60600.024.528122
ACACATC150700.023.9975786
TACACAT162400.023.8071585
ACATCTC153800.023.4208568
GCAGAGT450850.022.8422899
CACATCT171250.021.1331927
GAGTACT267900.020.9823912-13
Produced by FastQC (version 0.11.5)