##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140360_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9330254
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.52073287608248	34.0	31.0	34.0	31.0	34.0
2	32.509305641625616	34.0	31.0	34.0	31.0	34.0
3	32.597728636326515	34.0	31.0	34.0	31.0	34.0
4	36.11814973097196	37.0	35.0	37.0	35.0	37.0
5	36.18946526000257	37.0	37.0	37.0	35.0	37.0
6	36.16565176039152	37.0	37.0	37.0	35.0	37.0
7	36.15530263163254	37.0	36.0	37.0	35.0	37.0
8	36.14955841502279	37.0	36.0	37.0	35.0	37.0
9	37.89387480769548	39.0	38.0	39.0	35.0	39.0
10-11	37.8545477968767	39.0	38.0	39.0	35.0	39.0
12-13	37.8447532082192	39.0	38.0	39.0	35.0	39.0
14-15	39.22836339718083	40.0	39.0	41.0	36.0	41.0
16-17	39.23132248060985	40.0	39.0	41.0	36.0	41.0
18-19	39.30165802560145	40.0	39.0	41.0	36.0	41.0
20-21	39.323016962452314	40.0	39.0	41.0	36.0	41.0
22-23	39.28712767623957	40.0	39.0	41.0	36.0	41.0
24-25	39.2317815059649	40.0	39.0	41.0	36.0	41.0
26-27	39.18594584464368	40.0	39.0	41.0	36.0	41.0
28-29	39.16559591994279	40.0	39.0	41.0	36.0	41.0
30-31	39.07690209629561	40.0	39.0	41.0	35.5	41.0
32-33	38.96671676498141	40.0	38.0	41.0	35.0	41.0
34-35	38.937286613395926	40.0	38.0	41.0	35.0	41.0
36-37	38.86476030294056	40.0	38.0	41.0	35.0	41.0
38-39	38.80325419891763	40.0	38.0	41.0	35.0	41.0
40-41	38.78956612635146	40.0	38.0	41.0	35.0	41.0
42-43	38.739684092388	40.0	38.0	41.0	35.0	41.0
44-45	38.67118119073909	40.0	38.0	41.0	35.0	41.0
46-47	38.54690890318829	40.0	38.0	41.0	34.0	41.0
48-49	38.4693479884491	40.0	38.0	41.0	34.0	41.0
50-51	38.40058174719102	40.0	38.0	41.0	34.0	41.0
52-53	38.294971555579096	40.0	37.5	41.0	34.0	41.0
54-55	38.19449845892723	40.0	37.0	41.0	34.0	41.0
56-57	38.0487111612949	40.0	37.0	41.0	34.0	41.0
58-59	37.87630542867184	40.0	36.5	41.0	33.0	41.0
60-61	37.70473837038668	39.0	36.0	41.0	33.0	41.0
62-63	37.49396500880563	39.0	35.5	41.0	33.0	41.0
64-65	37.23432257728527	39.0	35.0	40.5	33.0	41.0
66-67	36.98821601410414	38.0	35.0	40.0	32.5	41.0
68-69	36.684945541021534	37.5	35.0	40.0	32.0	41.0
70-71	36.39507239154741	37.0	35.0	39.5	32.0	41.0
72-73	36.07135432496745	36.5	35.0	39.0	32.0	41.0
74-75	35.76262697556717	36.0	35.0	39.0	31.5	40.5
76-77	35.43430424250634	35.5	34.5	38.5	31.0	40.0
78-79	35.12171867910854	35.0	34.0	37.0	31.0	39.0
80-81	34.80948239291951	35.0	34.0	37.0	31.0	39.0
82-83	34.53545910590096	35.0	34.0	36.0	31.0	38.5
84-85	34.32229674225874	35.0	34.0	36.0	31.0	37.0
86-87	34.13972469050617	35.0	34.0	36.0	31.0	37.0
88-89	33.98775650499558	35.0	34.0	35.0	31.0	36.5
90-91	33.85777860713414	35.0	34.0	35.0	31.0	36.0
92-93	33.78030080367399	35.0	34.0	35.0	31.0	36.0
94-95	33.72265093694889	35.0	34.0	35.0	31.0	36.0
96-97	33.7188633099384	35.0	34.0	35.0	31.0	35.5
98-99	33.798442194813106	35.0	34.0	35.0	31.0	35.0
100	32.9586608012154	34.0	32.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	3.0
19	24.0
20	88.0
21	255.0
22	788.0
23	2375.0
24	6290.0
25	13271.0
26	24956.0
27	41042.0
28	61722.0
29	86787.0
30	116623.0
31	152104.0
32	198328.0
33	263253.0
34	378486.0
35	573707.0
36	1000840.0
37	2048316.0
38	2818736.0
39	1490969.0
40	51290.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.333086966335536	20.043998802176233	14.40881459390066	24.214099637587573
2	14.357162868993179	20.626512697603243	42.027704485205334	22.988619948198245
3	18.072509065669596	26.096545710331142	30.29074020921617	25.54020501478309
4	12.527097333041523	18.35769958674223	38.20697700191227	30.90822607830398
5	13.487103352170262	37.53244016722374	35.09705094845221	13.883405532153786
6	29.7403907878686	37.65322699194687	18.711339084223017	13.89504313596151
7	26.502890489369314	32.75955831427526	22.456644803024655	18.28090639333077
8	24.441735455433474	36.60468407398126	20.952044821073468	18.001535649511794
9	25.51266021268017	17.201514556838433	20.98869977173183	36.29712545874957
10-11	24.23445278124261	26.869391765754713	28.401322193372224	20.494833259630447
12-13	25.28960090475565	24.17912738495651	28.59851939722113	21.932752313066718
14-15	22.40117471614385	25.481465992244157	26.342975228755833	25.774384062856164
16-17	21.82792665665908	28.786552863405433	26.984372558346216	22.401147921589274
18-19	21.888294788116163	27.547036768773925	28.990711292532872	21.573957150577037
20-21	22.68891963004643	26.627810119826385	28.968324168596716	21.714946081530474
22-23	22.380394123579684	26.794339385092865	28.83063579251487	21.994630698812582
24-25	22.317215316793437	26.90074158949226	28.903477669402903	21.878565424311393
26-27	22.267780962462368	27.02768287391486	28.858844633946497	21.845691529676277
28-29	22.043871171292988	26.83786521749395	28.968526853264827	22.149736757948236
30-31	22.304518903983787	26.842228304311828	28.958541524965614	21.894711266738767
32-33	21.84933750218175	26.80164606304557	29.169593623471968	22.17942281130071
34-35	21.996588514767275	26.88113942893322	28.895660428419806	22.2266116278797
36-37	22.078899355109552	26.938281521332108	28.984367468889854	21.998451654668482
38-39	21.966531233326712	26.970535418384728	28.96908592033861	22.09384742794995
40-41	22.28144864881772	26.78232789500632	28.879886501415154	22.05633695476081
42-43	22.3520609401638	26.81142796767382	28.65041056988123	22.18610052228116
44-45	22.221905451208446	27.14836746488108	28.392749182441396	22.23697790146908
46-47	22.612502633503855	26.95813361593144	27.898211685979785	22.531152064584923
48-49	22.437498523693	27.109257355669218	27.802631209447316	22.650612911190464
50-51	22.29279848972301	27.261197836131252	27.89899214859058	22.54701152555516
52-53	22.464825672345192	27.18083739149954	28.05173711224498	22.302599823910285
54-55	22.434744322178705	27.099661645581552	27.826505743962223	22.63908828827752
56-57	22.60545597559147	27.415127082661066	27.50813485707831	22.471282084669156
58-59	22.29602596591057	27.680284597361798	27.8662967237795	22.157392712948138
60-61	22.59261171277242	27.88076799204971	27.38824996660034	22.13837032857753
62-63	22.302006561103788	28.157731985196087	27.37669380669069	22.16356764700944
64-65	22.323859634271685	28.02603691241589	27.35317446434133	22.296928988971096
66-67	22.40894982211568	27.934120965046933	27.272244069291663	22.38468514354572
68-69	22.427069771249336	27.723838242007098	27.41116786161417	22.437924125129392
70-71	22.455505113106682	27.309316320940425	27.609587458323336	22.62559110762956
72-73	22.527811206294075	27.33892583261263	27.486194299388938	22.64706866170435
74-75	22.37434544879506	27.48760035732961	27.49294343540737	22.645110758467958
76-77	22.556491421855785	27.396832885042322	27.44913278159854	22.597542911503353
78-79	22.503678880789053	27.421124396022133	27.3947991516926	22.68039757149621
80-81	22.523271077466394	27.463495080511997	27.374395815048143	22.638838026973477
82-83	22.566282906872175	27.461679714107596	27.453044985067	22.51899239395323
84-85	22.602984848281444	27.411299934084248	27.433771060440854	22.551944157193454
86-87	22.466374790760998	27.488533195167868	27.508901066166086	22.536190947905048
88-89	22.55283404243134	27.418902078393238	27.451130885577683	22.577132993597743
90-91	22.567135460392553	27.461111360987132	27.465755341726663	22.50599783689365
92-93	22.586583816669222	27.434665885690485	27.48752412392632	22.491226173713976
94-95	22.45821989382212	27.526470727088732	27.499044547614808	22.51626483147434
96-97	22.50948172630952	27.64705004261634	27.537649289175448	22.30581894189869
98-99	23.589868989225625	29.55994980396175	27.710233858418427	19.1399473483942
100	22.651572395803303	30.5405999066052	21.383176336517266	25.424651361074236
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1858.0
1	1629.0
2	2366.5
3	3412.5
4	4315.5
5	7233.0
6	9914.0
7	11870.0
8	13393.5
9	13149.5
10	11211.5
11	8859.5
12	8893.0
13	12175.5
14	21711.5
15	35753.0
16	48086.0
17	56421.5
18	58854.5
19	55466.0
20	49207.0
21	42262.5
22	37539.0
23	38166.0
24	43821.5
25	54132.0
26	69461.5
27	86837.0
28	104353.0
29	124181.0
30	144656.5
31	165206.0
32	187633.0
33	209095.5
34	229845.5
35	251032.0
36	273996.0
37	294055.5
38	309026.0
39	321901.5
40	336103.0
41	348369.5
42	355663.0
43	363456.5
44	370406.5
45	383414.0
46	401906.5
47	408104.5
48	403373.5
49	393888.5
50	382088.5
51	369985.5
52	354822.0
53	338942.0
54	321399.0
55	302391.0
56	282437.5
57	259482.5
58	233909.0
59	206448.0
60	175595.5
61	144145.0
62	114786.0
63	89044.0
64	68018.0
65	50863.0
66	37982.0
67	29302.5
68	22714.5
69	17046.5
70	12642.5
71	9249.0
72	6756.5
73	4880.5
74	3402.5
75	2372.5
76	1651.0
77	1081.0
78	745.0
79	533.5
80	360.5
81	229.5
82	162.5
83	127.0
84	99.0
85	71.0
86	52.0
87	40.0
88	26.0
89	17.0
90	10.0
91	5.5
92	3.0
93	1.5
94	1.0
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	7.288118844353005E-4
3	0.0
4	0.0
5	0.0
6	0.04086705463752648
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.008555884156545018
26-27	0.004858393644534717
28-29	0.001754558512031362
30-31	9.402619860030989E-4
32-33	0.0032375044583022082
34-35	0.0035184871910311543
36-37	0.015871957449470377
38-39	0.001240787291014798
40-41	0.002863373999484813
42-43	0.0025192808219026055
44-45	0.003870182106350252
46-47	0.006131491100505839
48-49	0.0014412552097515328
50-51	8.077575185781345E-5
52-53	0.01258289944425915
54-55	0.0049160140887455975
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0040731713718362384
64-65	4.8931423344154514E-5
66-67	2.472203012861451E-5
68-69	0.006754613694832427
70-71	2.1496213600034745E-4
72-73	0.002317174456617802
74-75	0.0
76-77	2.46788918969716E-4
78-79	7.734947219145541E-4
80-81	0.002428958265467004
82-83	0.008353335674049367
84-85	0.00531561282506271
86-87	0.0
88-89	0.0
90-91	0.001051324916560658
92-93	7.046825024797777E-6
94-95	9.999027951639843E-4
96-97	0.003626171433589401
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	231.0
20-21	855.0
22-23	1771.0
24-25	2460.0
26-27	4478.0
28-29	9092.0
30-31	22988.0
32-33	28698.0
34-35	38383.0
36-37	56894.0
38-39	67484.0
40-41	67999.0
42-43	78752.0
44-45	94785.0
46-47	92473.0
48-49	79147.0
50-51	68882.0
52-53	64509.0
54-55	69374.0
56-57	71319.0
58-59	71999.0
60-61	70991.0
62-63	72112.0
64-65	83362.0
66-67	87315.0
68-69	91312.0
70-71	83267.0
72-73	65771.0
74-75	67211.0
76-77	70527.0
78-79	72579.0
80-81	73567.0
82-83	70027.0
84-85	71165.0
86-87	75182.0
88-89	80664.0
90-91	85111.0
92-93	91443.0
94-95	111604.0
96-97	313484.0
98-99	600653.0
100-101	6000334.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.02140142120524
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.62979558946222	77.96102027122906
2	6.632339669366807	11.410463061207714
3	1.512597997504145	3.9034739859664573
4	0.5380538146836071	1.8513657271643738
5	0.25898015994931495	1.1138918149563979
6	0.1348981148874933	0.6962474935020554
7	0.07857600858187622	0.47314528634083497
8	0.05416735615542112	0.3727641510216698
9	0.03302276231510647	0.2556597863830341
>10	0.12385807601213991	1.6900918464087915
>50	0.0030548532595351015	0.17947281938683934
>100	6.351051154745122E-4	0.08348393095083194
>500	2.0492706825827238E-5	0.008919825481962079
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	4.287128731972356E-5	0.0	1.071782182993089E-5	0.0
3	0.0	4.287128731972356E-5	0.0	1.071782182993089E-5	0.0
4	0.0	7.502475280951623E-5	0.0	1.071782182993089E-5	0.0
5	0.0	8.574257463944712E-5	0.0	1.071782182993089E-5	0.0
6	0.0	8.574257463944712E-5	0.0	2.143564365986178E-5	1.071782182993089E-5
7	0.0	8.574257463944712E-5	0.0	2.143564365986178E-5	1.071782182993089E-5
8	0.0	1.071782182993089E-4	0.0	2.143564365986178E-5	1.071782182993089E-5
9	0.0	1.6076732744896334E-4	0.0	4.287128731972356E-5	1.071782182993089E-5
10-11	0.0	1.6076732744896334E-4	0.0	4.8230198234689E-5	1.071782182993089E-5
12-13	0.0	1.6076732744896334E-4	0.0	5.358910914965445E-5	2.143564365986178E-5
14-15	0.0	3.215346548979267E-4	0.0	5.358910914965445E-5	3.7512376404758114E-5
16-17	0.0	3.215346548979267E-4	0.0	5.358910914965445E-5	4.8230198234689E-5
18-19	0.0	3.483292094727539E-4	0.0	5.894802006461989E-5	5.358910914965445E-5
20-21	0.0	3.751237640475811E-4	0.0	6.430693097958534E-5	5.358910914965445E-5
22-23	0.0	4.34071784112201E-4	0.0	6.966584189455078E-5	6.430693097958534E-5
24-25	0.0	4.769430714319246E-4	0.0	8.574257463944712E-5	7.502475280951623E-5
26-27	0.0	4.930198041768209E-4	0.0	1.0181930738434345E-4	7.502475280951623E-5
28-29	0.0	4.930198041768209E-4	0.0	2.518688130033759E-4	7.502475280951623E-5
30-31	0.0	5.573267351564063E-4	0.0	9.485272319488837E-4	7.502475280951623E-5
32-33	0.0	5.680445569863371E-4	0.0	0.002315049515265072	9.6460396469378E-5
34-35	0.0	6.484282207108188E-4	0.0	0.004169232691843116	1.071782182993089E-4
36-37	0.0	7.448886171801968E-4	0.0	0.0068862005257305965	1.071782182993089E-4
38-39	0.0	7.663242608400586E-4	0.0	0.011167970346787987	1.1253712921427434E-4
40-41	0.0	8.788613900543329E-4	0.0	0.01572840353542358	1.1789604012923979E-4
42-43	0.0	8.949381227992292E-4	0.0	0.019806534741712283	1.1789604012923979E-4
44-45	0.0	9.592450537788146E-4	0.0	0.024227636246558777	1.2325495104420524E-4
46-47	0.0	0.0010181930738434345	0.0	0.029179269931986846	1.2861386195917068E-4
48-49	0.0	0.0010181930738434345	0.0	0.034238081835714224	1.5004950561903245E-4
50-51	0.0	0.0011146534703128125	0.0	0.03934512393767629	1.5004950561903245E-4
52-53	0.0	0.0011253712921427434	0.0	0.04401809425552616	1.7684406019385967E-4
54-55	0.0	0.0011414480248876397	0.0	0.04858924526599169	2.036386147686869E-4
56-57	0.0	0.0011896782231223288	0.0	0.05273704231417494	2.036386147686869E-4
58-59	0.0	0.0012218316886121214	0.0	0.05698129975882757	2.0899752568365236E-4
60-61	0.0	0.0012379084213570177	0.0	0.06122019829256524	2.143564365986178E-4
62-63	0.0	0.0012700618868468102	0.0	0.06530368840976891	2.143564365986178E-4
64-65	0.0	0.0014790594125304627	0.0	0.06930679486324809	2.143564365986178E-4
66-67	0.0	0.0015004950561903244	0.0	0.07345995082234631	2.143564365986178E-4
68-69	0.0	0.001554084165339979	0.0	0.07736123796844116	2.143564365986178E-4
70-71	0.0	0.0015808787199148061	0.0	0.08116070580715165	2.2507425842854868E-4
72-73	0.0	0.0016023143635746678	0.0	0.08418849047410713	2.3579208025847957E-4
74-75	0.0	0.0016451856508943915	0.0	0.08673933206963069	2.3579208025847957E-4
76-77	0.0	0.001709492581873977	0.0	0.0890007924757461	2.3579208025847957E-4
78-79	0.0	0.0017523638691937004	0.0	0.09053879990834118	2.3579208025847957E-4
80-81	0.0	0.0017577227801086658	0.0	0.09103717862343297	2.3579208025847957E-4
82-83	0.0	0.0017577227801086658	0.0	0.09142837912022544	2.3579208025847957E-4
84-85	0.0	0.0017684406019385967	0.0	0.09156771080401456	2.3579208025847957E-4
86-87	0.0	0.0017898762455984586	0.0	0.09162665882407918	2.3579208025847957E-4
88	0.0	0.0019613613948773526	0.0	0.09163737664590911	2.3579208025847957E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13410	0.0	53.33725	1
CTTATAC	8515	0.0	41.626568	1
GTATCAA	25980	0.0	40.19608	1
TCAACGC	31665	0.0	32.51084	4
ATCAACG	32240	0.0	31.944147	3
TATCAAC	32610	0.0	31.738047	2
CAACGCA	32545	0.0	31.657791	5
TATACAC	12475	0.0	30.788568	3
AACGCAG	33590	0.0	30.723331	6
TTATACA	12370	0.0	29.783745	2
ACGCAGA	38650	0.0	26.711105	7
CGCAGAG	39310	0.0	26.262636	8
GTGGTAT	6285	0.0	25.738773	1
TGGTATC	6060	0.0	24.52812	2
ACACATC	15070	0.0	23.997578	6
TACACAT	16240	0.0	23.807158	5
ACATCTC	15380	0.0	23.420856	8
GCAGAGT	45085	0.0	22.842289	9
CACATCT	17125	0.0	21.133192	7
GAGTACT	26790	0.0	20.98239	12-13
>>END_MODULE