FastQCFastQC Report
Fri 27 May 2016
SRR2140359_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140359_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9298492
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC114800.054.1280141
GGTATCA137000.052.453571
TATACAC157000.041.705323
TTATACA160600.039.7709162
GTATCAA257150.039.3371471
TCAACGC315750.031.4470084
ATCAACG317950.031.2294163
TACACAT210700.031.1563515
TATCAAC325450.030.7050532
CAACGCA325700.030.5641825
ACACATC210100.029.9576916
AACGCAG334750.029.6999976
ACATCTC214100.029.286838
GTGGTAT58850.027.1513841
TGGTATC56150.027.0265982
CACATCT232000.026.9179277
ATACACA267900.025.8294774
ACGCAGA386150.025.7357277
CATCTCC242350.025.7160429
CGCAGAG393350.025.2431688
Produced by FastQC (version 0.11.5)