##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140359_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9298492
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.49415087951896	34.0	31.0	34.0	31.0	34.0
2	32.48431229493987	34.0	31.0	34.0	31.0	34.0
3	32.57247454748576	34.0	31.0	34.0	31.0	34.0
4	36.09848790535067	37.0	35.0	37.0	35.0	37.0
5	36.17342704601994	37.0	37.0	37.0	35.0	37.0
6	36.15060216215705	37.0	37.0	37.0	35.0	37.0
7	36.136316082220645	37.0	36.0	37.0	35.0	37.0
8	36.13053643537038	37.0	36.0	37.0	35.0	37.0
9	37.874523309801205	39.0	38.0	39.0	35.0	39.0
10-11	37.831054056937404	39.0	38.0	39.0	35.0	39.0
12-13	37.82090832578014	39.0	38.0	39.0	35.0	39.0
14-15	39.195609944064046	40.0	39.0	41.0	36.0	41.0
16-17	39.19509701142938	40.0	39.0	41.0	36.0	41.0
18-19	39.26946724264536	40.0	39.0	41.0	36.0	41.0
20-21	39.293763286800285	40.0	39.0	41.0	36.0	41.0
22-23	39.253466964675894	40.0	39.0	41.0	36.0	41.0
24-25	39.20137928203872	40.0	39.0	41.0	36.0	41.0
26-27	39.15839921988038	40.0	39.0	41.0	36.0	41.0
28-29	39.1418839354263	40.0	39.0	41.0	36.0	41.0
30-31	39.05755869233781	40.0	39.0	41.0	35.5	41.0
32-33	38.93712011746182	40.0	38.0	41.0	35.0	41.0
34-35	38.89299150307209	40.0	38.0	41.0	35.0	41.0
36-37	38.81653400109318	40.0	38.0	41.0	35.0	41.0
38-39	38.74639809035533	40.0	38.0	41.0	35.0	41.0
40-41	38.7494372442348	40.0	38.0	41.0	35.0	41.0
42-43	38.70156352360445	40.0	38.0	41.0	35.0	41.0
44-45	38.634485671035215	40.0	38.0	41.0	35.0	41.0
46-47	38.510498908679914	40.0	38.0	41.0	34.0	41.0
48-49	38.438806654614595	40.0	38.0	41.0	34.0	41.0
50-51	38.37143378460758	40.0	38.0	41.0	34.0	41.0
52-53	38.28027294576879	40.0	38.0	41.0	34.0	41.0
54-55	38.17349324720092	40.0	37.0	41.0	34.0	41.0
56-57	38.037926608189935	40.0	37.0	41.0	34.0	41.0
58-59	37.87097528328211	39.5	36.5	41.0	33.0	41.0
60-61	37.68510346843574	39.0	36.0	41.0	33.0	41.0
62-63	37.475751919398455	39.0	35.5	41.0	33.0	41.0
64-65	37.225969873573916	39.0	35.0	40.5	33.0	41.0
66-67	36.97711153402602	38.0	35.0	40.0	32.0	41.0
68-69	36.68479296563932	37.5	35.0	40.0	32.0	41.0
70-71	36.403596713844195	37.0	35.0	39.5	32.0	41.0
72-73	36.08360711167862	36.5	35.0	39.0	32.0	41.0
74-75	35.77698049638873	36.0	35.0	39.0	31.0	40.5
76-77	35.44915766101073	35.5	34.5	38.5	31.0	40.0
78-79	35.13451968504562	35.0	34.0	37.0	31.0	39.0
80-81	34.81887593063102	35.0	34.0	37.0	31.0	39.0
82-83	34.54152370944712	35.0	34.0	36.0	31.0	38.5
84-85	34.32275421337072	35.0	34.0	36.0	31.0	37.0
86-87	34.13384565323413	35.0	34.0	36.0	31.0	37.0
88-89	33.979046190488006	35.0	34.0	35.0	31.0	36.5
90-91	33.843001649076825	35.0	34.0	35.0	31.0	36.0
92-93	33.764009890119056	35.0	34.0	35.0	31.0	36.0
94-95	33.706467071428996	35.0	34.0	35.0	31.0	36.0
96-97	33.70438899265368	35.0	34.0	35.0	31.0	36.0
98-99	33.780843729148046	35.0	34.0	35.0	31.0	35.0
100	32.9339643805678	34.0	32.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	4.0
19	29.0
20	92.0
21	245.0
22	846.0
23	2461.0
24	6258.0
25	13311.0
26	24979.0
27	41780.0
28	63062.0
29	87970.0
30	117707.0
31	154489.0
32	200537.0
33	267648.0
34	381806.0
35	577599.0
36	997253.0
37	2028764.0
38	2819408.0
39	1465149.0
40	47093.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.03796615623265	20.21770841981689	14.333420946106099	24.410904477844365
2	14.22105346816784	20.69588018856125	42.29267774891861	22.790388594352304
3	17.927283262705394	26.235146516230806	30.508559882613223	25.32901033845058
4	12.315308761893865	18.69927941003767	38.19124649459288	30.794165333475576
5	13.273700724805702	37.67021577262206	35.3225662827908	13.733517219781444
6	29.29864249742517	37.974019636163874	18.76746206933799	13.959875797072963
7	26.156187476420907	32.846562646932426	22.41441945640218	18.582830420244488
8	24.164187053126465	36.763004151640935	21.19442593487202	17.878382860360585
9	25.192192454432394	17.21923296809848	20.986155604586205	36.602418972882916
10-11	23.96082074383674	26.992893041151188	28.597733912122525	20.448552302889546
12-13	25.126386084969475	24.250926924494852	28.686737591428802	21.935949399106867
14-15	22.193555686233854	25.56274178651764	26.326860312403344	25.91684221484516
16-17	21.71913467258992	28.982586638779708	26.946390877144378	22.35188781148599
18-19	21.79409306369248	27.61434864922183	28.967240064302903	21.62431822278279
20-21	22.474039768261537	26.672229112542517	28.977380032066876	21.876351087129066
22-23	22.064975111240425	27.070973099272415	28.88150766911543	21.982544120371728
24-25	22.019890876642382	27.14011125587943	29.030384185855198	21.809613681622995
26-27	21.89571067020186	27.105996012789035	29.220913958983648	21.777379358025456
28-29	21.583991459171	26.8230457221659	29.532561049072935	22.06040176959016
30-31	22.05406898208684	26.744659585528186	28.850792981866636	22.350478450518345
32-33	22.129911905165798	26.871503022357413	29.302692751845367	21.695892320631422
34-35	22.09605674317083	27.298381861155264	29.069121895569715	21.53643950010419
36-37	21.81301660379966	27.061584235485505	28.906581651887496	22.21881750882734
38-39	21.709462860002134	26.98311885833627	29.265215674288957	22.042202607372637
40-41	22.05289676509618	26.83243336642348	29.20083789089289	21.913831977587453
42-43	22.308762810654315	26.790870042751486	28.957442932479548	21.942924214114655
44-45	21.975994309252876	27.25537227729146	28.695035128724054	22.07359828473161
46-47	22.52297934954669	26.905229166603966	28.081400271187356	22.49039121266198
48-49	22.304602538488307	27.01804819443498	28.022521415785224	22.654827851291483
50-51	22.051291841117642	27.1755414307511	28.280064501666775	22.493102226464483
52-53	22.252195871530642	27.109303013829738	28.606242215071926	22.032258899567694
54-55	22.20791332103415	27.072684511932955	28.161517351859843	22.55788481517305
56-57	22.549395137052798	27.517613138572877	27.621930485028578	22.311061239345744
58-59	21.98724138940099	27.959798790906053	28.247464730778628	21.805495088914327
60-61	22.451459987926782	28.38352406092946	27.367137505371115	21.797878445772646
62-63	21.960921082113057	28.850131363605453	27.367463380416158	21.821484173865336
64-65	22.031300042191187	28.657397126604618	27.349433500146265	21.961869331057933
66-67	22.16650832823693	28.407113325999156	27.340490909601474	22.085887436162444
68-69	22.188781088287822	28.07663501365592	27.5516361998415	22.182947698214754
70-71	22.313077322404947	27.522026508304076	27.767822059511143	22.397074109779833
72-73	22.367261070765327	27.48719050777162	27.711560871701007	22.43398754976205
74-75	22.241959237329322	27.61904062376479	27.668499440053917	22.47050069885197
76-77	22.364692176311713	27.584460200464683	27.620852528647156	22.429995094576448
78-79	22.3573281238045	27.58213069482241	27.6006034659181	22.45993771545499
80-81	22.354565273893442	27.604249475752695	27.57646739287708	22.464717857476778
82-83	22.398383565938783	27.582778236932352	27.66774751511594	22.35109068201292
84-85	22.509349249965172	27.515792134129164	27.635848118926482	22.339010496979185
86-87	22.30811027474722	27.598368869149155	27.6927530158593	22.400767840244324
88-89	22.410389367734894	27.561239936014225	27.64413116541315	22.38423953083773
90-91	22.39971017999788	27.61226605650548	27.667672408065773	22.320351355430866
92-93	22.4095026158209	27.581848072256587	27.6863656317118	22.32228368021071
94-95	22.309843076538492	27.6699804625505	27.68452770889813	22.33564875201287
96-97	22.347926364538452	27.80949959165436	27.734566366056235	22.10800767775095
98-99	23.45419923946021	29.646325769732663	27.955093304326944	18.944381686480188
100	22.506117182994103	30.714224766651704	21.60542415189564	25.17423389845855
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1938.0
1	1688.5
2	2493.0
3	3634.5
4	4524.0
5	7453.5
6	10214.0
7	12574.0
8	14751.0
9	14785.5
10	12344.5
11	9415.0
12	9260.5
13	12574.0
14	22546.5
15	37059.0
16	49446.0
17	57728.0
18	60194.5
19	56916.0
20	50791.5
21	43803.0
22	39282.0
23	40540.5
24	47104.5
25	58584.0
26	74593.0
27	92558.0
28	110650.5
29	130520.5
30	150682.5
31	170234.0
32	191828.0
33	213059.0
34	232759.0
35	251229.0
36	272067.5
37	292384.0
38	306006.5
39	317587.5
40	331427.5
41	343733.5
42	352628.5
43	362277.0
44	370395.0
45	388434.0
46	418935.0
47	429347.5
48	419092.5
49	402726.0
50	384525.5
51	368800.5
52	351250.0
53	332202.0
54	311983.0
55	290647.0
56	269537.5
57	246277.5
58	220750.5
59	194078.0
60	163963.5
61	133440.0
62	105577.5
63	82039.0
64	62398.0
65	46657.0
66	34876.5
67	26682.5
68	20526.0
69	15070.5
70	11198.0
71	8290.0
72	5926.5
73	4231.5
74	2963.5
75	2057.0
76	1444.5
77	981.5
78	643.0
79	445.0
80	337.5
81	250.5
82	186.5
83	135.0
84	94.5
85	67.5
86	50.5
87	38.5
88	30.0
89	20.5
90	14.5
91	12.0
92	8.0
93	4.0
94	2.0
95	1.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	8.711089927269927E-4
3	0.0
4	0.0
5	0.0
6	0.03982366172923523
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.008391662109079667
26-27	0.004853554486099517
28-29	0.0018414046622427728
30-31	9.219913373791679E-4
32-33	0.003265027586780271
34-35	0.003340886847091189
36-37	0.015152704774148146
38-39	0.001239402219857121
40-41	0.002629318704304885
42-43	0.002593664257063519
44-45	0.003842218942105681
46-47	0.005956250122077576
48-49	0.0016574433861212803
50-51	5.784902515136776E-6
52-53	0.012486975245358571
54-55	0.004904941067279931
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0041200404784861875
64-65	0.0
66-67	1.2425195662662398E-5
68-69	0.006751677395415541
70-71	1.977742107533028E-4
72-73	0.0023333096779562116
74-75	0.0
76-77	2.2294349543008454E-4
78-79	7.942368059396204E-4
80-81	0.0024860904243215027
82-83	0.00819190322811596
84-85	0.005524599933132586
86-87	0.0
88-89	0.0
90-91	0.0010756351484945959
92-93	0.0
94-95	8.726829186671059E-4
96-97	0.0035075461141205497
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	264.0
20-21	913.0
22-23	1881.0
24-25	2429.0
26-27	4596.0
28-29	9632.0
30-31	22745.0
32-33	28474.0
34-35	38018.0
36-37	55806.0
38-39	65522.0
40-41	66746.0
42-43	77665.0
44-45	94180.0
46-47	91075.0
48-49	77862.0
50-51	67919.0
52-53	64411.0
54-55	67763.0
56-57	70561.0
58-59	71589.0
60-61	71771.0
62-63	77401.0
64-65	96064.0
66-67	102036.0
68-69	105118.0
70-71	92489.0
72-73	65090.0
74-75	65945.0
76-77	69982.0
78-79	72645.0
80-81	72529.0
82-83	69899.0
84-85	70689.0
86-87	74122.0
88-89	79658.0
90-91	84278.0
92-93	90785.0
94-95	111056.0
96-97	308518.0
98-99	596497.0
100-101	5941869.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.01005168740004
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.07509336696992	79.24448581294837
2	6.403740371894299	11.143795615024265
3	1.4429214051114612	3.7664599811881234
4	0.48354306477004616	1.6829242823490214
5	0.23681161015186575	1.0302495219745131
6	0.11684575454148477	0.6100053085264708
7	0.06429079991766402	0.39157620767021684
8	0.047188567837938206	0.32847037813067087
9	0.02941276709027352	0.2303285746314742
>10	0.09764991631745965	1.3247853630641333
>50	0.002028914282958694	0.11986069747751496
>100	3.9768038701480305E-4	0.05779197862188896
>500	3.789086505046475E-5	0.02154511447566218
>1k	3.788986247084781E-5	0.047721163917651084
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	2.150886401795044E-5	0.0	0.0	0.0
4	0.0	8.603545607180176E-5	0.0	0.0	1.075443200897522E-5
5	0.0	9.678988808077697E-5	0.0	0.0	1.075443200897522E-5
6	0.0	9.678988808077697E-5	0.0	0.0	1.075443200897522E-5
7	0.0	9.678988808077697E-5	0.0	0.0	1.075443200897522E-5
8	0.0	1.0754432008975219E-4	0.0	0.0	1.075443200897522E-5
9	0.0	2.3659750419745482E-4	0.0	0.0	1.075443200897522E-5
10-11	1.075443200897522E-5	2.3659750419745482E-4	0.0	5.37721600448761E-6	1.075443200897522E-5
12-13	2.150886401795044E-5	2.4735193620643E-4	0.0	1.075443200897522E-5	1.075443200897522E-5
14-15	2.150886401795044E-5	3.656506883051574E-4	0.0	1.075443200897522E-5	4.301772803590088E-5
16-17	2.6886080022438047E-5	3.656506883051574E-4	0.0	1.075443200897522E-5	5.3772160044876094E-5
18-19	3.2263296026925655E-5	3.71027904309645E-4	0.0	1.075443200897522E-5	5.3772160044876094E-5
20-21	3.2263296026925655E-5	3.7640512031413264E-4	0.0	1.075443200897522E-5	5.3772160044876094E-5
22-23	6.452659205385131E-5	4.3017728035900875E-4	0.0	1.075443200897522E-5	6.452659205385131E-5
24-25	9.678988808077697E-5	4.6781779239042203E-4	0.0	1.075443200897522E-5	7.528102406282653E-5
26-27	9.678988808077697E-5	4.8394944040388487E-4	0.0	1.075443200897522E-5	7.528102406282653E-5
28-29	1.0754432008975219E-4	4.8394944040388487E-4	0.0	1.9895699216604154E-4	7.528102406282653E-5
30-31	1.0754432008975219E-4	5.162127364308105E-4	0.0	8.065824006731414E-4	7.528102406282653E-5
32-33	1.1829875209872741E-4	5.269671684397857E-4	0.0	0.0023982383380014736	8.603545607180176E-5
34-35	1.1829875209872741E-4	5.91493760493637E-4	0.0	0.004344790531625988	8.603545607180176E-5
36-37	1.2905318410770262E-4	6.452659205385132E-4	0.0	0.007269996038067248	9.678988808077697E-5
38-39	1.2905318410770262E-4	6.667747845564636E-4	0.0	0.011281399177415004	9.678988808077697E-5
40-41	1.3980761611667784E-4	7.850735366551909E-4	0.0	0.01552402260495573	9.678988808077697E-5
42-43	1.3980761611667784E-4	8.065824006731414E-4	0.0	0.019470899152249636	9.678988808077697E-5
44-45	1.3980761611667784E-4	9.195039367673812E-4	0.0	0.0244340695243917	9.678988808077697E-5
46-47	1.3980761611667784E-4	0.0010109166088436705	0.0	0.02993496149698252	1.0754432008975219E-4
48-49	1.3980761611667784E-4	0.0010109166088436705	0.0	0.034849736925084196	1.2905318410770262E-4
50-51	1.3980761611667784E-4	0.0011614786569693236	0.0	0.03978602121720382	1.3980761611667784E-4
52-53	1.3980761611667784E-4	0.0011722330889782989	0.0	0.04480834096539525	1.4518483212116545E-4
54-55	1.4518483212116545E-4	0.0011776103049827864	0.0	0.04957793156137576	1.5056204812565307E-4
56-57	1.5056204812565307E-4	0.001182987520987274	0.0	0.054239977837266515	1.5593926413014068E-4
58-59	1.5593926413014068E-4	0.0011991191690007368	0.0	0.05933220139351628	1.666936961391159E-4
60-61	1.613164801346283E-4	0.001226005249023175	0.0	0.0639673615893846	1.720709121436035E-4
62-63	1.666936961391159E-4	0.0012528913290456131	0.0	0.06836054706505099	1.720709121436035E-4
64-65	1.720709121436035E-4	0.0014410938892026792	0.0	0.07239883628442118	1.720709121436035E-4
66-67	1.7744812814809112E-4	0.0014572255372161422	0.0	0.07671674073602472	1.8282534415257873E-4
68-69	1.828253441525787E-4	0.00148411161723858	0.0	0.08098087302758339	1.9357977616155393E-4
70-71	1.828253441525787E-4	0.0015163749132655058	0.0	0.08475030144672921	1.9357977616155393E-4
72-73	1.828253441525787E-4	0.001527129345274481	0.0	0.08758947149709867	2.312202881929672E-4
74-75	1.828253441525787E-4	0.0015755242893148694	0.0	0.0897887528429341	2.3659750419745482E-4
76-77	1.828253441525787E-4	0.0016776913934001341	0.0	0.0920902012928548	2.3659750419745482E-4
78-79	1.828253441525787E-4	0.0017314635534450102	0.0	0.09362808507013826	2.3659750419745482E-4
80-81	1.828253441525787E-4	0.0017314635534450102	0.0	0.0941227889425511	2.4735193620643E-4
82-83	1.828253441525787E-4	0.0017314635534450102	0.0	0.09432174593471715	2.6886080022438046E-4
84-85	1.828253441525787E-4	0.0017314635534450102	0.0	0.09445079911882487	2.6886080022438046E-4
86-87	1.828253441525787E-4	0.0017422179854539855	0.0	0.09448306241485178	2.6886080022438046E-4
88	1.828253441525787E-4	0.0018282534415257872	0.0	0.09449919406286525	2.6886080022438046E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	11480	0.0	54.128014	1
GGTATCA	13700	0.0	52.45357	1
TATACAC	15700	0.0	41.70532	3
TTATACA	16060	0.0	39.770916	2
GTATCAA	25715	0.0	39.337147	1
TCAACGC	31575	0.0	31.447008	4
ATCAACG	31795	0.0	31.229416	3
TACACAT	21070	0.0	31.156351	5
TATCAAC	32545	0.0	30.705053	2
CAACGCA	32570	0.0	30.564182	5
ACACATC	21010	0.0	29.957691	6
AACGCAG	33475	0.0	29.699997	6
ACATCTC	21410	0.0	29.28683	8
GTGGTAT	5885	0.0	27.151384	1
TGGTATC	5615	0.0	27.026598	2
CACATCT	23200	0.0	26.917927	7
ATACACA	26790	0.0	25.829477	4
ACGCAGA	38615	0.0	25.735727	7
CATCTCC	24235	0.0	25.716042	9
CGCAGAG	39335	0.0	25.243168	8
>>END_MODULE