FastQCFastQC Report
Fri 27 May 2016
SRR2140358_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140358_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9611182
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA131050.050.9593051
GTATCAA243800.038.602831
CTTATAC81500.035.6153531
TCAACGC297050.030.9549394
ATCAACG302000.030.4896583
TATCAAC307700.030.1592772
CAACGCA306850.029.9801275
AACGCAG316200.029.1472236
TATACAC116500.027.9341243
ACGCAGA364450.025.2988647
TTATACA120600.025.0522372
CGCAGAG372000.024.7740238
GTGGTAT63650.024.632451
TGGTATC58900.024.4606462
GCAGAGT430200.021.353539
TACACAT150250.021.236375
ACACATC139450.020.99716
ACATCTC142400.020.7896778
GAGTACT248650.020.00530212-13
GTACATG312000.019.8698251
Produced by FastQC (version 0.11.5)