##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140358_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9611182
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.51208561028186	34.0	31.0	34.0	31.0	34.0
2	32.501451018199425	34.0	31.0	34.0	31.0	34.0
3	32.59119252970134	34.0	31.0	34.0	31.0	34.0
4	36.10923422322041	37.0	35.0	37.0	35.0	37.0
5	36.18218737299949	37.0	37.0	37.0	35.0	37.0
6	36.161033575266806	37.0	37.0	37.0	35.0	37.0
7	36.152034057829724	37.0	36.0	37.0	35.0	37.0
8	36.147049967423364	37.0	36.0	37.0	35.0	37.0
9	37.89247763698575	39.0	38.0	39.0	35.0	39.0
10-11	37.8531645223241	39.0	38.0	39.0	35.0	39.0
12-13	37.84551582729367	39.0	38.0	39.0	35.0	39.0
14-15	39.23152761023566	40.0	39.0	41.0	36.0	41.0
16-17	39.235858607193165	40.0	39.0	41.0	36.0	41.0
18-19	39.303093365623496	40.0	39.0	41.0	36.0	41.0
20-21	39.324841837357056	40.0	39.0	41.0	36.0	41.0
22-23	39.28934959804202	40.0	39.0	41.0	36.0	41.0
24-25	39.23582234663331	40.0	39.0	41.0	36.0	41.0
26-27	39.19361786330574	40.0	39.0	41.0	36.0	41.0
28-29	39.17375862538883	40.0	39.0	41.0	36.0	41.0
30-31	39.092563337189716	40.0	39.0	41.0	35.5	41.0
32-33	38.98554225573763	40.0	38.0	41.0	35.0	41.0
34-35	38.95563188239882	40.0	38.0	41.0	35.0	41.0
36-37	38.883578575994	40.0	38.0	41.0	35.0	41.0
38-39	38.82794998827261	40.0	38.0	41.0	35.0	41.0
40-41	38.81046967012813	40.0	38.0	41.0	35.0	41.0
42-43	38.76300724414715	40.0	38.0	41.0	35.0	41.0
44-45	38.69808255767282	40.0	38.0	41.0	35.0	41.0
46-47	38.57381722671934	40.0	38.0	41.0	34.5	41.0
48-49	38.50090126871685	40.0	38.0	41.0	34.0	41.0
50-51	38.436784218823234	40.0	38.0	41.0	34.0	41.0
52-53	38.32297115547129	40.0	38.0	41.0	34.0	41.0
54-55	38.22736406407425	40.0	37.0	41.0	34.0	41.0
56-57	38.08460126958673	40.0	37.0	41.0	34.0	41.0
58-59	37.90378539738505	40.0	36.5	41.0	33.0	41.0
60-61	37.73735439900213	39.0	36.0	41.0	33.0	41.0
62-63	37.52447365748756	39.0	36.0	41.0	33.0	41.0
64-65	37.25991107525799	39.0	35.0	40.5	33.0	41.0
66-67	37.01016614565651	38.0	35.0	40.0	32.5	41.0
68-69	36.70446830745819	37.5	35.0	40.0	32.0	41.0
70-71	36.40949391640792	37.0	35.0	39.5	32.0	41.0
72-73	36.085245971963815	36.5	35.0	39.0	32.0	41.0
74-75	35.77439398790216	36.0	35.0	39.0	32.0	40.5
76-77	35.44682995386516	35.5	34.5	38.5	31.0	40.0
78-79	35.13359255254011	35.0	34.0	37.0	31.0	39.0
80-81	34.818175347320945	35.0	34.0	37.0	31.0	39.0
82-83	34.54329538891487	35.0	34.0	36.0	31.0	38.5
84-85	34.32989660736631	35.0	34.0	36.0	31.0	37.0
86-87	34.14571807289002	35.0	34.0	36.0	31.0	37.0
88-89	33.992834692763964	35.0	34.0	35.0	31.0	36.5
90-91	33.86091360032426	35.0	34.0	35.0	31.0	36.0
92-93	33.78399949505916	35.0	34.0	35.0	31.0	36.0
94-95	33.72734786440531	35.0	34.0	35.0	31.0	36.0
96-97	33.725080452156014	35.0	34.0	35.0	31.0	35.5
98-99	33.79975654507089	35.0	34.0	35.0	31.0	35.0
100	32.96404856414879	34.0	32.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	3.0
19	19.0
20	98.0
21	252.0
22	864.0
23	2494.0
24	6202.0
25	13524.0
26	25393.0
27	41485.0
28	62622.0
29	88190.0
30	117985.0
31	154787.0
32	202242.0
33	268741.0
34	387001.0
35	585756.0
36	1026746.0
37	2108170.0
38	2917091.0
39	1548927.0
40	52589.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.36002210758261	20.152079109520557	14.340400587565608	24.14749819533123
2	14.410974137627866	20.846102415268337	41.80713437142406	22.93578907567974
3	18.04504378337649	26.08929890204972	30.324469976741675	25.54118733783212
4	12.556520103354613	18.405800660106113	38.3773296562275	30.66034958031177
5	13.55195437980469	37.59812268667891	35.07123265379846	13.778690279717937
6	29.688138201467336	37.736601646536364	18.857100745325635	13.718159406670665
7	26.698277069355257	32.688175086061214	22.602131558844686	18.01141628573884
8	24.548988875665863	36.63419338016906	20.90711631514209	17.909701429022988
9	25.468958968834425	17.344131034039307	21.042448264948057	36.14446173217821
10-11	24.14116702815533	26.926917001467665	28.547513718916157	20.384402251460852
12-13	25.254791762345153	24.228403956974283	28.69120052039385	21.825603760286715
14-15	22.388073600104544	25.63028147838632	26.357569755728278	25.62407516578086
16-17	21.858320860014928	28.885359781970628	26.976921256927604	22.27939810108684
18-19	21.909427997513728	27.759291208927266	28.885469029719758	21.445811763839245
20-21	22.702669013124247	26.778172825268076	28.890229163557102	21.62892899805058
22-23	22.415878545491665	26.878130651340935	28.705733043356148	22.000257759811248
24-25	22.2932611145267	27.089801964703085	28.71242473210309	21.904512188667123
26-27	22.25282090659448	27.204799770943687	28.686049689602665	21.85632963285917
28-29	22.06585897325242	27.129131057199253	28.753074349927054	22.051935619621272
30-31	22.616581418954514	26.942521169355892	28.636022696094948	21.804874715594647
32-33	21.968553370980406	27.331514775270065	28.67395198471248	22.025979869037045
34-35	22.298297176818338	27.247694283779524	28.657748006007424	21.796260533394722
36-37	22.20915662620121	27.191751829199546	28.606119874811327	21.992971669787913
38-39	22.072366411177267	27.105178073170897	28.78604912456975	22.03640639108208
40-41	22.334116137279985	26.9444027933083	28.662696896435108	22.058784172976615
42-43	22.366063276897087	26.95201065254364	28.467935894229708	22.213990176329563
44-45	22.28654986575313	27.21018049717338	28.2710245608616	22.232245076211886
46-47	22.598943136498097	27.10091669509896	27.787061909257083	22.513078259145864
48-49	22.456622081596965	27.197337731210254	27.746710265620578	22.599329921572206
50-51	22.366635618329937	27.386947088447066	27.771405018472496	22.4750122747505
52-53	22.559473948900298	27.269678988473167	27.868591559158524	22.30225550346801
54-55	22.508929463482865	27.220013351619905	27.686888618425236	22.584168566471995
56-57	22.560906820413418	27.48146301718461	27.49781568533964	22.459814477062334
58-59	22.35180059975156	27.694994015300097	27.70457692437803	22.248628460570313
60-61	22.584175580723706	27.86199070846729	27.34239558352005	22.211438127288954
62-63	22.365319191902817	28.026643349800107	27.356142463611157	22.25189499468592
64-65	22.42893997426344	27.930313333605177	27.2886714567898	22.352075235341587
66-67	22.46993037825871	27.85722851930595	27.251468297801985	22.421372804633364
68-69	22.44030308641303	27.70532821654147	27.368404173307724	22.485964523737774
70-71	22.53212404255659	27.342223795156045	27.503061989399463	22.622590172887904
72-73	22.60872605856388	27.367423862151053	27.401130903357974	22.622719175927095
74-75	22.430618119088894	27.53567780586814	27.40292419384258	22.63077988120038
76-77	22.609617617678047	27.43926692368232	27.338743428005273	22.612372030634354
78-79	22.54992126008249	27.42771359381199	27.32799901053673	22.694366135568785
80-81	22.566052696706468	27.470691611965993	27.297426756847077	22.665828934480466
82-83	22.625753822647106	27.470456526739984	27.365523338888785	22.538266311724122
84-85	22.664271974010997	27.44477650523663	27.341830973091042	22.549120547661335
86-87	22.53998214499973	27.47651575896701	27.418228010150603	22.56527408588266
88-89	22.630810763663252	27.433317496061942	27.361522162584972	22.57434957768983
90-91	22.628533331585388	27.465480712305133	27.36908464142655	22.53690131468293
92-93	22.64174358921733	27.454517778131247	27.39098890160242	22.512749731049002
94-95	22.520648962334548	27.498184769486013	27.419451378351052	22.56171488982839
96-97	22.60780595945776	27.606753531574533	27.44632900706258	22.33911150190512
98-99	23.659622936240936	29.552524531073615	27.628860216685197	19.158992316000248
100	22.723602061711826	30.47417008782654	21.326865624048537	25.475362226413097
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1412.0
1	1308.0
2	2165.5
3	3217.0
4	4112.0
5	6904.5
6	9440.5
7	11144.5
8	12199.0
9	11811.0
10	10101.5
11	8039.5
12	8111.0
13	11008.5
14	19873.0
15	33276.0
16	45148.0
17	53171.0
18	55667.0
19	52802.5
20	46955.5
21	40443.0
22	36788.5
23	38288.0
24	44923.5
25	56606.0
26	72887.5
27	91198.0
28	109423.0
29	129464.5
30	149404.0
31	169460.0
32	192823.5
33	215775.5
34	237985.0
35	259451.0
36	283504.0
37	306173.5
38	322651.0
39	336469.0
40	350279.5
41	362692.0
42	371248.5
43	381368.0
44	401226.0
45	420535.5
46	419521.5
47	411420.0
48	405325.5
49	397620.5
50	390978.0
51	380885.0
52	366594.5
53	349064.5
54	330235.0
55	310735.0
56	288953.0
57	264998.5
58	239050.0
59	210315.5
60	177752.5
61	145910.0
62	116729.5
63	90644.5
64	69356.5
65	52092.5
66	38823.0
67	29696.5
68	22953.0
69	17008.5
70	12279.0
71	8904.0
72	6332.0
73	4452.0
74	3064.0
75	2079.5
76	1426.0
77	958.5
78	665.5
79	470.5
80	326.0
81	207.5
82	150.5
83	123.0
84	95.0
85	67.0
86	50.0
87	42.0
88	29.0
89	19.5
90	13.0
91	6.5
92	4.5
93	2.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	7.907456127664631E-4
3	0.0
4	0.0
5	0.0
6	0.042721072184461806
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.00844096034814642
26-27	0.00503898498184664
28-29	0.0017970335507726566
30-31	9.440968933422443E-4
32-33	0.0034772621825880567
34-35	0.0036354195623028293
36-37	0.01585189356612851
38-39	0.0013895861865374133
40-41	0.0031248504345091173
42-43	0.0026100052641384523
44-45	0.00404011311013446
46-47	0.006212003269533169
48-49	0.0016071692606378532
50-51	7.266468150483189E-5
52-53	0.013113277367953436
54-55	0.005034987486608608
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.004205505429507213
64-65	6.519481663276239E-5
66-67	2.3949475707058876E-5
68-69	0.0068977535105934976
70-71	2.569880087559486E-4
72-73	0.0024155305641839213
74-75	0.0
76-77	2.0123524481871125E-4
78-79	7.682503586967274E-4
80-81	0.0025780314651946833
82-83	0.00869293102120369
84-85	0.005448529417055101
86-87	0.0
88-89	0.0
90-91	0.0010947068020418038
92-93	1.3682719757991E-5
94-95	8.183988830381074E-4
96-97	0.003599909330377966
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	222.0
20-21	849.0
22-23	1690.0
24-25	2453.0
26-27	4719.0
28-29	9943.0
30-31	22607.0
32-33	28887.0
34-35	38602.0
36-37	57124.0
38-39	66950.0
40-41	67547.0
42-43	78852.0
44-45	95374.0
46-47	92643.0
48-49	79617.0
50-51	70120.0
52-53	67009.0
54-55	71878.0
56-57	74190.0
58-59	74992.0
60-61	73939.0
62-63	74493.0
64-65	84502.0
66-67	86485.0
68-69	90024.0
70-71	84487.0
72-73	69871.0
74-75	71748.0
76-77	74828.0
78-79	78178.0
80-81	78618.0
82-83	74746.0
84-85	75886.0
86-87	79644.0
88-89	85848.0
90-91	89918.0
92-93	95598.0
94-95	117205.0
96-97	322408.0
98-99	618515.0
100-101	6177973.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.36396588850175
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.79103639892632	78.41073970538595
2	6.491206406970902	11.21212657413718
3	1.5362986250322257	3.980425261405059
4	0.5352539585214061	1.8490661846171301
5	0.2355269616501638	1.0170521240888604
6	0.13334349324749176	0.6909643741368026
7	0.08224652931151236	0.49721955163349785
8	0.04695376689301212	0.32440908178278094
9	0.03320199181449568	0.2580710119647678
>10	0.11195471506899325	1.5238617211586525
>50	0.002285286019249683	0.13091516654001506
>100	6.725432518976353E-4	0.09176517709920874
>500	1.9323292401899383E-5	0.013384066050126492
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.040454753640083E-5
2	0.0	1.040454753640083E-5	0.0	0.0	1.040454753640083E-5
3	0.0	2.080909507280166E-5	0.0	0.0	1.040454753640083E-5
4	0.0	6.242728521840498E-5	0.0	0.0	2.080909507280166E-5
5	0.0	7.283183275480581E-5	0.0	0.0	2.080909507280166E-5
6	1.3525911797321078E-4	7.283183275480581E-5	0.0	0.0	3.121364260920249E-5
7	1.3525911797321078E-4	7.283183275480581E-5	0.0	0.0	3.121364260920249E-5
8	1.3525911797321078E-4	8.323638029120663E-5	0.0	1.040454753640083E-5	7.283183275480581E-5
9	1.3525911797321078E-4	1.6647276058241327E-4	0.0	1.040454753640083E-5	7.283183275480581E-5
10-11	1.4566366550961162E-4	1.6647276058241327E-4	0.0	3.121364260920249E-5	7.283183275480581E-5
12-13	1.5606821304601246E-4	1.6647276058241327E-4	0.0	4.161819014560332E-5	8.843865405940705E-5
14-15	1.768773081188141E-4	2.3410231956901868E-4	0.0	4.161819014560332E-5	1.1965229666860955E-4
16-17	2.809227834828224E-4	2.393045933372191E-4	0.0	4.161819014560332E-5	1.5086593927781202E-4
18-19	2.809227834828224E-4	2.4970914087361993E-4	0.0	4.161819014560332E-5	1.6127048681421286E-4
20-21	2.8612505725102285E-4	2.8612505725102285E-4	0.0	4.161819014560332E-5	1.6647276058241327E-4
22-23	2.9132733101922323E-4	3.3294552116482654E-4	0.0	4.682046391380374E-5	1.6647276058241327E-4
24-25	2.965296047874236E-4	3.485523424694278E-4	0.0	6.242728521840498E-5	1.8207958188701451E-4
26-27	3.121364260920249E-4	3.6415916377402903E-4	0.0	1.768773081188141E-4	1.8728185565521495E-4
28-29	3.121364260920249E-4	3.6415916377402903E-4	0.0	4.578000916016365E-4	1.9248412942341535E-4
30-31	3.121364260920249E-4	3.953728063832315E-4	0.0	0.0016491207845195314	1.9768640319161576E-4
32-33	3.121364260920249E-4	4.057773539196324E-4	0.0	0.004167021288328533	2.2890004580081825E-4
34-35	3.225409736284257E-4	4.734069129062378E-4	0.0	0.007678556081863812	2.2890004580081825E-4
36-37	3.225409736284257E-4	5.306319243564424E-4	0.0	0.012776784374700218	2.3410231956901868E-4
38-39	3.225409736284257E-4	5.462387456610435E-4	0.0	0.02016921539931301	2.393045933372191E-4
40-41	3.433500687012274E-4	6.502842210250519E-4	0.0	0.02822233519248725	2.393045933372191E-4
42-43	3.537546162376282E-4	6.710933160978536E-4	0.0	0.035989329928410474	2.445068671054195E-4
44-45	3.537546162376282E-4	7.543296963890601E-4	0.0	0.044349383873908535	2.4970914087361993E-4
46-47	3.537546162376282E-4	8.219592553756655E-4	0.0	0.053874747143483497	2.4970914087361993E-4
48-49	3.537546162376282E-4	8.219592553756655E-4	0.0	0.062739421644497	2.7051823594642155E-4
50-51	3.7976598507863033E-4	9.364092782760747E-4	0.0	0.07081335053274404	2.7051823594642155E-4
52-53	3.849682588468307E-4	9.572183733488764E-4	0.0	0.07973004777143955	2.809227834828224E-4
54-55	3.849682588468307E-4	9.676229208852771E-4	0.0	0.08706525378460214	2.965296047874236E-4
56-57	3.849682588468307E-4	9.884320159580789E-4	0.0	0.09256925943135819	3.121364260920249E-4
58-59	3.953728063832315E-4	9.884320159580789E-4	0.0	0.09831256967145144	3.277432473966261E-4
60-61	4.213841752242336E-4	0.0010092411110308806	0.0	0.10375414803298907	3.3294552116482654E-4
62-63	5.202273768200415E-4	0.0010248479323354816	0.0	0.10892520815858028	3.3294552116482654E-4
64-65	5.202273768200415E-4	0.0011705115978450933	0.0	0.11359685000242425	3.3294552116482654E-4
66-67	5.306319243564424E-4	0.0011965229666860955	0.0	0.11812282818075862	3.3294552116482654E-4
68-69	5.306319243564424E-4	0.0012329388830634985	0.0	0.12248753587227876	3.3294552116482654E-4
70-71	5.410364718928431E-4	0.0012381411568316988	0.0	0.12685744583756714	3.433500687012274E-4
72-73	5.826546620384465E-4	0.0012381411568316988	0.0	0.13016609195414258	3.745637113104299E-4
74-75	5.826546620384465E-4	0.0013005684420501038	0.0	0.13244989013838254	3.7976598507863033E-4
76-77	5.826546620384465E-4	0.0013838048223413103	0.0	0.13510304976016477	4.0057508015143195E-4
78-79	5.826546620384465E-4	0.001404613917414112	0.0	0.13675737281845252	4.057773539196324E-4
80-81	5.826546620384465E-4	0.0014150184649505129	0.0	0.13727239792150436	4.109796276878328E-4
82-83	5.878569358066469E-4	0.0014254230124869137	0.0	0.13759493889513277	4.3178872276063444E-4
84-85	5.930592095748473E-4	0.001441029833791515	0.0	0.13772499573933777	4.421932702970353E-4
86-87	5.982614833430478E-4	0.0014878502977053187	0.0	0.1378290412147018	4.473955440652357E-4
88	6.13868304647649E-4	0.0015502775829237237	0.0	0.1378394457622382	4.473955440652357E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13105	0.0	50.959305	1
GTATCAA	24380	0.0	38.60283	1
CTTATAC	8150	0.0	35.615353	1
TCAACGC	29705	0.0	30.954939	4
ATCAACG	30200	0.0	30.489658	3
TATCAAC	30770	0.0	30.159277	2
CAACGCA	30685	0.0	29.980127	5
AACGCAG	31620	0.0	29.147223	6
TATACAC	11650	0.0	27.934124	3
ACGCAGA	36445	0.0	25.298864	7
TTATACA	12060	0.0	25.052237	2
CGCAGAG	37200	0.0	24.774023	8
GTGGTAT	6365	0.0	24.63245	1
TGGTATC	5890	0.0	24.460646	2
GCAGAGT	43020	0.0	21.35353	9
TACACAT	15025	0.0	21.23637	5
ACACATC	13945	0.0	20.9971	6
ACATCTC	14240	0.0	20.789677	8
GAGTACT	24865	0.0	20.005302	12-13
GTACATG	31200	0.0	19.869825	1
>>END_MODULE