FastQCFastQC Report
Fri 27 May 2016
SRR2140357_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140357_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10296672
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA130600.050.6309551
GTATCAA248550.038.9837881
TCAACGC302650.031.4400044
ATCAACG304900.031.2219123
CAACGCA313000.030.4546135
TATCAAC316550.030.2206252
AACGCAG322200.029.6772256
ACGCAGA369850.025.8092757
CGCAGAG380400.025.0711758
GTGGTAT64000.023.4755151
TGGTATC59800.022.711232
GCAGAGT441500.021.5342649
CTTATAC67450.020.6387671
GTACATG316350.020.177711
GAGTACT258150.019.95103512-13
TACATGG316750.019.683262
ACATGGG323750.018.7331183
AGAGTAC398850.018.635610-11
CAGAGTA430250.018.58700410-11
GTACTTT285750.017.92077414-15
Produced by FastQC (version 0.11.5)