##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140357_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10296672
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.51154003934475	34.0	31.0	34.0	31.0	34.0
2	32.49877756618837	34.0	31.0	34.0	31.0	34.0
3	32.589456088336114	34.0	31.0	34.0	31.0	34.0
4	36.11110949246514	37.0	35.0	37.0	35.0	37.0
5	36.18333690730364	37.0	37.0	37.0	35.0	37.0
6	36.16283678843028	37.0	37.0	37.0	35.0	37.0
7	36.1519929934643	37.0	36.0	37.0	35.0	37.0
8	36.14478105158638	37.0	36.0	37.0	35.0	37.0
9	37.892481959219445	39.0	38.0	39.0	35.0	39.0
10-11	37.851316813821015	39.0	38.0	39.0	35.0	39.0
12-13	37.84454685941244	39.0	38.0	39.0	35.0	39.0
14-15	39.23176289387483	40.0	39.0	41.0	36.0	41.0
16-17	39.23509464999954	40.0	39.0	41.0	36.0	41.0
18-19	39.301486392885	40.0	39.0	41.0	36.0	41.0
20-21	39.32253900369112	40.0	39.0	41.0	36.0	41.0
22-23	39.286334400044744	40.0	39.0	41.0	36.0	41.0
24-25	39.2368691809703	40.0	39.0	41.0	36.0	41.0
26-27	39.19519761159254	40.0	39.0	41.0	36.0	41.0
28-29	39.17426809544883	40.0	39.0	41.0	36.0	41.0
30-31	39.09459186010454	40.0	39.0	41.0	35.5	41.0
32-33	38.992298340535186	40.0	38.0	41.0	35.0	41.0
34-35	38.967707751005726	40.0	38.0	41.0	35.0	41.0
36-37	38.89866333885074	40.0	38.0	41.0	35.0	41.0
38-39	38.850931161867514	40.0	38.0	41.0	35.0	41.0
40-41	38.82749617746994	40.0	38.0	41.0	35.0	41.0
42-43	38.77615392792903	40.0	38.0	41.0	35.0	41.0
44-45	38.712810301972254	40.0	38.0	41.0	35.0	41.0
46-47	38.59074676376417	40.0	38.0	41.0	34.5	41.0
48-49	38.517028302425146	40.0	38.0	41.0	34.0	41.0
50-51	38.45410894704631	40.0	38.0	41.0	34.0	41.0
52-53	38.34291177064459	40.0	38.0	41.0	34.0	41.0
54-55	38.246203764133526	40.0	37.0	41.0	34.0	41.0
56-57	38.10705031262839	40.0	37.0	41.0	34.0	41.0
58-59	37.93592001101248	40.0	37.0	41.0	33.0	41.0
60-61	37.7698575154348	39.0	36.0	41.0	33.0	41.0
62-63	37.55916236358499	39.0	36.0	41.0	33.0	41.0
64-65	37.29676340775903	39.0	35.0	40.5	33.0	41.0
66-67	37.04094129370918	38.0	35.0	40.0	32.5	41.0
68-69	36.72801475963563	37.5	35.0	40.0	32.0	41.0
70-71	36.42655357661153	37.0	35.0	39.5	32.0	41.0
72-73	36.096116102412054	36.5	35.0	39.0	32.0	41.0
74-75	35.78124914963678	36.0	35.0	39.0	32.0	40.5
76-77	35.45020064081752	35.5	34.5	38.5	31.0	40.0
78-79	35.13210043659252	35.0	34.0	37.0	31.0	39.0
80-81	34.8160708139355	35.0	34.0	37.0	31.0	39.0
82-83	34.540280561159605	35.0	34.0	36.0	31.0	38.5
84-85	34.32534404033533	35.0	34.0	36.0	31.0	37.0
86-87	34.13878883725616	35.0	34.0	36.0	31.0	37.0
88-89	33.98600844859182	35.0	34.0	35.0	31.0	36.5
90-91	33.85376109891136	35.0	34.0	35.0	31.0	36.0
92-93	33.778006439023436	35.0	34.0	35.0	31.0	36.0
94-95	33.719794853981796	35.0	34.0	35.0	31.0	36.0
96-97	33.71819400534302	35.0	34.0	35.0	31.0	35.5
98-99	33.796697359349196	35.0	34.0	35.0	31.0	35.0
100	32.95000133697913	34.0	32.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	8.0
19	28.0
20	93.0
21	258.0
22	898.0
23	2715.0
24	6811.0
25	14393.0
26	26719.0
27	44318.0
28	66683.0
29	94353.0
30	126217.0
31	164777.0
32	216062.0
33	287833.0
34	411262.0
35	623883.0
36	1098867.0
37	2265536.0
38	3140778.0
39	1648844.0
40	55334.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.57453981247533	19.97429849178453	14.332407597328533	24.118754098411603
2	14.444066110353686	20.86420354987014	41.71847178321547	22.9732585565607
3	18.102645204198016	25.89967904192733	30.264565094430512	25.733110659444137
4	12.57204269496008	18.285315876819226	38.57596901212353	30.566672416097163
5	13.59867537783082	37.56730329955155	35.027094191210516	13.806927131407118
6	29.83489453519595	37.68802603787594	18.915837923374657	13.56124150355345
7	26.852006162767932	32.64160497683135	22.678725708656156	17.827663151744563
8	24.585477715518177	36.58935625025251	20.907182437199126	17.917983597030187
9	25.588316302587867	17.30046368379997	21.071351986350543	36.039868027261626
10-11	24.175262647970143	26.855274208987133	28.60550476891951	20.36395837412321
12-13	25.26020543336721	24.25308876499125	28.72774815008189	21.758957651559648
14-15	22.429820042825487	25.639721261393973	26.479210952820488	25.451247742960053
16-17	21.859912600886965	28.80862865205379	27.114557014149813	22.21690173290943
18-19	21.873305277666415	27.842165895932204	28.950587141165613	21.333941685235775
20-21	22.704532547157328	26.848404310208977	28.93612020489979	21.510942937733898
22-23	22.413945335463023	26.813326632400475	28.768657934214037	22.004070097922465
24-25	22.23561143713485	26.999371033465323	28.800774522225662	21.964243007174165
26-27	22.143936835833237	27.139402416889276	28.78558867730103	21.931072069976462
28-29	22.028562118216584	27.05149647283833	28.871230471471677	22.04871093747341
30-31	22.39087502531902	26.913630981131487	28.79373860644116	21.901755387108334
32-33	22.089735287452974	27.17558559104758	28.692416339289434	22.04226278221001
34-35	22.23146778988642	27.185111370102366	28.742757987822753	21.840662852188466
36-37	22.343062316121518	27.06266663334082	28.58312729365314	22.011143756884525
38-39	22.137135820303012	27.19377733089909	28.652649863759542	22.016436985038354
40-41	22.416023947957175	27.03336602091856	28.530053504415815	22.02055652670845
42-43	22.308100000708407	27.105253942487888	28.351150504937788	22.23549555186591
44-45	22.36469436261535	27.255477569915588	28.139023967597197	22.240804099871866
46-47	22.55198373824301	27.251742371358684	27.74485541809074	22.451418472307562
48-49	22.456454266116268	27.364050198123262	27.704604943252946	22.474890592507528
50-51	22.43315226537705	27.503171549776923	27.67022372011442	22.393452464731606
52-53	22.626030605282665	27.433894649430556	27.571006366246646	22.36906837904013
54-55	22.559889150793953	27.36231635593725	27.54952326237597	22.528271230892827
56-57	22.561486280079453	27.529440005717582	27.499335112385104	22.409738601817864
58-59	22.475206129177067	27.619572377006797	27.570231635361413	22.334989858454723
60-61	22.628179861132168	27.634673770572153	27.451479340934753	22.285667027360926
62-63	22.498308761860244	27.737522032675077	27.42267363309736	22.34149557236732
64-65	22.519441807028375	27.66997970821224	27.37362887594945	22.43694960880994
66-67	22.579620117976713	27.661169732952708	27.28970601502359	22.46950413404699
68-69	22.526069525602267	27.5411655418042	27.44072244725851	22.49204248533502
70-71	22.559259092733967	27.326844240688224	27.49797202828605	22.615924638291755
72-73	22.61181943143327	27.348630803777414	27.402916369254733	22.636633395534588
74-75	22.45114490104599	27.52435776577611	27.38281761852599	22.641679714651914
76-77	22.610347233807865	27.428395672422628	27.344085924842847	22.61717116892666
78-79	22.550890427798045	27.456988369080797	27.307403193334718	22.684718009786444
80-81	22.60042883616398	27.442705342347303	27.335707582943947	22.621158238544773
82-83	22.627694929963845	27.43482838892287	27.369747393529277	22.567729287584005
84-85	22.665681172363573	27.424559041569367	27.338327278579815	22.571432507487245
86-87	22.51747361964258	27.486816716473385	27.40830356555125	22.58740609833279
88-89	22.628424077834648	27.448697799014298	27.351631837461937	22.571246285689117
90-91	22.607825144951523	27.4680050040378	27.3873806348851	22.536789216125573
92-93	22.65117220060544	27.44932135053233	27.398023042114033	22.501483406748196
94-95	22.531794539365034	27.504803907950176	27.41729818441862	22.546103368266166
96-97	22.6040652308958	27.606661024739136	27.457045448821805	22.332228295543263
98-99	23.675260981525607	29.558651835863902	27.629970758671778	19.13611642393871
100	22.771563745812927	30.53510111017339	21.2230414682855	25.47029367572818
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1608.0
1	1512.5
2	2325.5
3	3318.5
4	4239.0
5	7323.5
6	10101.5
7	11892.5
8	12955.5
9	12250.5
10	10371.5
11	8283.0
12	8315.0
13	11447.5
14	20613.5
15	34448.5
16	46852.0
17	55141.5
18	57761.5
19	54910.0
20	49269.5
21	43031.0
22	39189.5
23	40744.0
24	47417.5
25	59719.5
26	77046.5
27	96033.0
28	114893.0
29	135446.0
30	157542.0
31	180071.0
32	204635.0
33	228492.5
34	252278.0
35	276510.5
36	302823.5
37	327007.0
38	345341.0
39	361049.5
40	377135.5
41	391217.5
42	401493.5
43	413966.0
44	427788.0
45	440167.0
46	444061.0
47	443267.0
48	442040.5
49	436337.0
50	429108.0
51	417022.0
52	399805.5
53	379327.5
54	357101.5
55	334249.5
56	309207.0
57	281505.0
58	252239.0
59	221285.0
60	186279.5
61	151778.5
62	120734.0
63	93808.0
64	71443.5
65	53351.0
66	39566.0
67	30195.0
68	23105.5
69	16974.0
70	12352.0
71	8893.0
72	6350.0
73	4504.0
74	3184.5
75	2171.0
76	1483.0
77	1016.5
78	695.0
79	487.0
80	348.5
81	244.5
82	176.5
83	133.0
84	100.5
85	71.0
86	53.0
87	38.0
88	26.5
89	20.5
90	14.0
91	9.5
92	5.5
93	2.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.5
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	6.798313086014587E-4
3	0.0
4	0.0
5	0.0
6	0.04157654045889779
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.008369650394408238
26-27	0.0049513412887150915
28-29	0.0018427272635777275
30-31	0.0010906535663592581
32-33	0.003358353391522013
34-35	0.0038246489072694036
36-37	0.015122268509504155
38-39	0.0014011523413527934
40-41	0.002677767083331214
42-43	0.00262757795290275
44-45	0.003786983736400381
46-47	0.006060188480570386
48-49	0.0015518552429429383
50-51	1.565037888263077E-5
52-53	0.012564453940157556
54-55	0.004810269820112861
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.004098806891014972
64-65	1.1051267881573065E-5
66-67	1.1151549106292552E-5
68-69	0.006495393533323536
70-71	2.728396795748066E-4
72-73	0.002403328960498277
74-75	0.0
76-77	2.393333364076965E-4
78-79	7.424989048141154E-4
80-81	0.0023151165205881705
82-83	0.008216349031949398
84-85	0.005067202022123101
86-87	0.0
88-89	0.0
90-91	0.0011155054505601718
92-93	6.344089186728368E-6
94-95	8.488761265936684E-4
96-97	0.003574171502127222
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	240.0
20-21	890.0
22-23	1905.0
24-25	2550.0
26-27	5116.0
28-29	11031.0
30-31	24846.0
32-33	30758.0
34-35	41355.0
36-37	61037.0
38-39	71914.0
40-41	73201.0
42-43	84504.0
44-45	101450.0
46-47	98187.0
48-49	84188.0
50-51	74822.0
52-53	71778.0
54-55	76680.0
56-57	78989.0
58-59	79775.0
60-61	77915.0
62-63	75353.0
64-65	80103.0
66-67	83773.0
68-69	86091.0
70-71	82382.0
72-73	74489.0
74-75	76166.0
76-77	79706.0
78-79	83273.0
80-81	83364.0
82-83	79676.0
84-85	80459.0
86-87	84908.0
88-89	91422.0
90-91	96239.0
92-93	102277.0
94-95	126217.0
96-97	349456.0
98-99	671389.0
100-101	6656798.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.92605169317835
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.45071303455902	77.72072643892358
2	6.727452582819258	11.561268767894676
3	1.5637811422403292	4.0310861779488
4	0.5875171653907065	2.019321212959658
5	0.2544069151075256	1.093009086931564
6	0.13229604655565652	0.6820606161086683
7	0.0779241231286413	0.46869985624680593
8	0.04840840746076596	0.3327634657486573
9	0.03830468673975183	0.29622334436018266
>10	0.11630557080453534	1.5398936412203788
>50	0.002320483831787587	0.1387349847731042
>100	5.149624288915893E-4	0.07528272545242799
>500	3.658726396340567E-5	0.020450027704324313
>1k	1.829166913544833E-5	0.020479653727162237
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.711875837163696E-6
2	0.0	0.0	0.0	0.0	9.711875837163696E-6
3	0.0	0.0	0.0	0.0	1.9423751674327393E-5
4	0.0	4.855937918581849E-5	0.0	0.0	1.9423751674327393E-5
5	0.0	4.855937918581849E-5	0.0	0.0	1.9423751674327393E-5
6	3.8847503348654785E-5	4.855937918581849E-5	0.0	9.711875837163696E-6	1.9423751674327393E-5
7	3.8847503348654785E-5	4.855937918581849E-5	0.0	9.711875837163696E-6	2.913562751149109E-5
8	3.8847503348654785E-5	4.855937918581849E-5	0.0	9.711875837163696E-6	2.913562751149109E-5
9	3.8847503348654785E-5	8.740688253447327E-5	0.0	2.913562751149109E-5	2.913562751149109E-5
10-11	4.855937918581849E-5	8.740688253447327E-5	0.0	2.913562751149109E-5	2.913562751149109E-5
12-13	4.855937918581849E-5	8.740688253447327E-5	0.0	3.399156543007294E-5	2.913562751149109E-5
14-15	4.855937918581849E-5	1.9423751674327395E-4	0.0	3.8847503348654785E-5	4.855937918581849E-5
16-17	9.711875837163697E-5	1.9423751674327395E-4	0.0	5.341531710440033E-5	7.769500669730957E-5
18-19	9.711875837163697E-5	2.0880533049901949E-4	0.0	6.798313086014588E-5	7.769500669730957E-5
20-21	9.711875837163697E-5	2.1366126841760135E-4	0.0	7.283906877872772E-5	7.769500669730957E-5
22-23	9.711875837163697E-5	2.5736470968483797E-4	0.0	1.0683063420880066E-4	8.255094461589142E-5
24-25	9.711875837163697E-5	2.816443992777472E-4	0.0	1.5539001339461917E-4	8.740688253447327E-5
26-27	9.711875837163697E-5	2.9135627511491094E-4	0.0	2.0394939258043765E-4	9.711875837163697E-5
28-29	9.711875837163697E-5	2.9135627511491094E-4	0.0	4.3703441267236643E-4	9.711875837163697E-5
30-31	1.1168657212738253E-4	3.496275301378931E-4	0.0	0.0013111032380170992	9.711875837163697E-5
32-33	1.1654251004596436E-4	3.496275301378931E-4	0.0	0.0033457412259028938	1.0683063420880068E-4
34-35	1.2625438588312806E-4	4.2246659891662086E-4	0.0	0.005997083329448583	1.0683063420880068E-4
36-37	1.2625438588312806E-4	4.953056676953486E-4	0.0	0.009794426781779587	1.0683063420880068E-4
38-39	1.2625438588312806E-4	5.050175435325123E-4	0.0	0.01557784884281057	1.0683063420880068E-4
40-41	1.2625438588312806E-4	5.341531710440034E-4	0.0	0.021628347489363556	1.0683063420880068E-4
42-43	1.3596626172029177E-4	5.730006743926582E-4	0.0	0.027319506729941483	1.0683063420880068E-4
44-45	1.408221996388736E-4	6.701194327642952E-4	0.0	0.033986709492154356	1.1168657212738253E-4
46-47	1.4567813755745547E-4	7.186788119501136E-4	0.0	0.04114921792206258	1.1654251004596436E-4
48-49	1.4567813755745547E-4	7.283906877872773E-4	0.0	0.04799609038726299	1.1654251004596436E-4
50-51	1.6995782715036468E-4	9.226282045305512E-4	0.0	0.05460987783237147	1.1654251004596436E-4
52-53	1.9423751674327395E-4	9.226282045305512E-4	0.0	0.061408190918386056	1.2625438588312806E-4
54-55	1.9423751674327395E-4	9.274841424491331E-4	0.0	0.067842308660507	1.3596626172029177E-4
56-57	1.9423751674327395E-4	9.323400803677149E-4	0.0	0.07382968011411842	1.3596626172029177E-4
58-59	1.9423751674327395E-4	9.323400803677149E-4	0.0	0.07944800028591763	1.3596626172029177E-4
60-61	1.990934546618558E-4	9.371960182862967E-4	0.0	0.08492549825807795	1.3596626172029177E-4
62-63	2.3308502009192873E-4	9.420519562048786E-4	0.0	0.09014563152055344	1.3596626172029177E-4
64-65	2.3308502009192873E-4	0.0010974419695994977	0.0	0.09549687510683064	1.3596626172029177E-4
66-67	2.3794095801051056E-4	0.0011120097833552433	0.0	0.10116375465781566	1.3596626172029177E-4
68-69	2.4279689592909243E-4	0.0011702810383782255	0.0	0.1064178794857212	1.3596626172029177E-4
70-71	2.476528338476743E-4	0.0011897047900525529	0.0	0.11108929176339695	1.408221996388736E-4
72-73	2.865003371963291E-4	0.0012042726038082984	0.0	0.11436219392052112	1.7481376506894654E-4
74-75	2.9135627511491094E-4	0.001252831982994117	0.0	0.11727575667167023	1.8452564090611025E-4
76-77	3.010681509520746E-4	0.0013256710517728447	0.0	0.12008248878861053	1.9423751674327395E-4
78-79	3.010681509520746E-4	0.0013402388655285903	0.0	0.12203943176979902	1.9423751674327395E-4
80-81	3.010681509520746E-4	0.0013402388655285903	0.0	0.12264642400962175	2.0880533049901949E-4
82-83	3.0592408887065645E-4	0.001345094803447172	0.0	0.1229814837260039	2.2337314425476502E-4
84-85	3.107800267892383E-4	0.0013596626172029177	0.0	0.12312230592564277	2.2337314425476502E-4
86-87	3.156359647078201E-4	0.001379086368877245	0.0	0.12320971280817725	2.2337314425476502E-4
88	3.20491902626402E-4	0.0014859170030860458	0.0	0.12322428062193298	2.2337314425476502E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13060	0.0	50.630955	1
GTATCAA	24855	0.0	38.983788	1
TCAACGC	30265	0.0	31.440004	4
ATCAACG	30490	0.0	31.221912	3
CAACGCA	31300	0.0	30.454613	5
TATCAAC	31655	0.0	30.220625	2
AACGCAG	32220	0.0	29.677225	6
ACGCAGA	36985	0.0	25.809275	7
CGCAGAG	38040	0.0	25.071175	8
GTGGTAT	6400	0.0	23.475515	1
TGGTATC	5980	0.0	22.71123	2
GCAGAGT	44150	0.0	21.534264	9
CTTATAC	6745	0.0	20.638767	1
GTACATG	31635	0.0	20.17771	1
GAGTACT	25815	0.0	19.951035	12-13
TACATGG	31675	0.0	19.68326	2
ACATGGG	32375	0.0	18.733118	3
AGAGTAC	39885	0.0	18.6356	10-11
CAGAGTA	43025	0.0	18.587004	10-11
GTACTTT	28575	0.0	17.920774	14-15
>>END_MODULE