FastQCFastQC Report
Fri 27 May 2016
SRR2140356_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140356_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9872817
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA130950.053.3001561
GTATCAA245700.039.0231931
TCAACGC299350.031.5040024
ATCAACG303400.031.0974643
CAACGCA311500.030.3297425
TATCAAC313550.030.2672352
AACGCAG319850.029.5777996
ACGCAGA365250.025.9144787
CGCAGAG373750.025.3478438
GTGGTAT59050.022.157831
GCAGAGT432100.021.836479
GTACATG311050.020.8001331
TGGTATC58350.020.6035442
GAGTACT249800.020.46711712-13
TACATGG313350.020.2271882
ACATGGG320650.019.4750293
CAGAGTA420800.019.39546810-11
AGAGTAC389150.019.0742310-11
GTACTTT277300.018.25431314-15
AGTACTT267800.017.291712-13
Produced by FastQC (version 0.11.5)