##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140356_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9872817
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.50719647695283	34.0	31.0	34.0	31.0	34.0
2	32.49295981076121	34.0	31.0	34.0	31.0	34.0
3	32.58268901368272	34.0	31.0	34.0	31.0	34.0
4	36.10871294383356	37.0	35.0	37.0	35.0	37.0
5	36.18128422718663	37.0	37.0	37.0	35.0	37.0
6	36.16046838506173	37.0	37.0	37.0	35.0	37.0
7	36.150329637427696	37.0	36.0	37.0	35.0	37.0
8	36.14367388760472	37.0	36.0	37.0	35.0	37.0
9	37.888235748722984	39.0	38.0	39.0	35.0	39.0
10-11	37.847573899121194	39.0	38.0	39.0	35.0	39.0
12-13	37.83948137598418	39.0	38.0	39.0	35.0	39.0
14-15	39.22288380307262	40.0	39.0	41.0	36.0	41.0
16-17	39.22731885945015	40.0	39.0	41.0	36.0	41.0
18-19	39.29440133449248	40.0	39.0	41.0	36.0	41.0
20-21	39.315822516521834	40.0	39.0	41.0	36.0	41.0
22-23	39.279516397713536	40.0	39.0	41.0	36.0	41.0
24-25	39.22719777767243	40.0	39.0	41.0	36.0	41.0
26-27	39.18731073998937	40.0	39.0	41.0	36.0	41.0
28-29	39.16773146652672	40.0	39.0	41.0	36.0	41.0
30-31	39.0880098060361	40.0	39.0	41.0	35.5	41.0
32-33	38.984601031795805	40.0	38.0	41.0	35.0	41.0
34-35	38.95902997042073	40.0	38.0	41.0	35.0	41.0
36-37	38.88866795805289	40.0	38.0	41.0	35.0	41.0
38-39	38.842176941047576	40.0	38.0	41.0	35.0	41.0
40-41	38.81894007675581	40.0	38.0	41.0	35.0	41.0
42-43	38.76640508168826	40.0	38.0	41.0	35.0	41.0
44-45	38.70068438495393	40.0	38.0	41.0	35.0	41.0
46-47	38.576848148289926	40.0	38.0	41.0	34.5	41.0
48-49	38.50263449004324	40.0	38.0	41.0	34.0	41.0
50-51	38.440080876844604	40.0	38.0	41.0	34.0	41.0
52-53	38.3258397163372	40.0	38.0	41.0	34.0	41.0
54-55	38.230838508576724	40.0	37.0	41.0	34.0	41.0
56-57	38.0894255043963	40.0	37.0	41.0	34.0	41.0
58-59	37.91580044652146	40.0	36.5	41.0	33.0	41.0
60-61	37.74849807395425	39.0	36.0	41.0	33.0	41.0
62-63	37.535660735533014	39.0	36.0	41.0	33.0	41.0
64-65	37.2678343976012	39.0	35.0	40.5	33.0	41.0
66-67	37.01447781331753	38.0	35.0	40.0	32.5	41.0
68-69	36.701860980253045	37.5	35.0	40.0	32.0	41.0
70-71	36.398341615592265	37.0	35.0	39.5	32.0	41.0
72-73	36.068718762706816	36.5	35.0	39.0	32.0	41.0
74-75	35.75616789140211	36.0	35.0	39.0	31.5	40.5
76-77	35.43050456638494	35.5	34.5	38.0	31.0	40.0
78-79	35.116747868079685	35.0	34.0	37.0	31.0	39.0
80-81	34.8024470052662	35.0	34.0	37.0	31.0	39.0
82-83	34.52860890469698	35.0	34.0	36.0	31.0	38.5
84-85	34.3143244538209	35.0	34.0	36.0	31.0	37.0
86-87	34.12777202391791	35.0	34.0	36.0	31.0	37.0
88-89	33.97727479597027	35.0	34.0	35.0	31.0	36.5
90-91	33.84627415479055	35.0	34.0	35.0	31.0	36.0
92-93	33.76928908737048	35.0	34.0	35.0	31.0	36.0
94-95	33.71029596539894	35.0	34.0	35.0	31.0	36.0
96-97	33.71002510378541	35.0	34.0	35.0	31.0	35.5
98-99	33.785969881767926	35.0	34.0	35.0	31.0	35.0
100	32.939342494917405	34.0	32.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	7.0
19	19.0
20	72.0
21	268.0
22	851.0
23	2613.0
24	6375.0
25	13757.0
26	25396.0
27	42591.0
28	63795.0
29	90030.0
30	120684.0
31	159497.0
32	207337.0
33	277083.0
34	397449.0
35	602841.0
36	1066955.0
37	2198790.0
38	2988832.0
39	1556667.0
40	50908.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.5622207927079	20.091955517862836	14.233789606350447	24.11203408307882
2	14.485237544805155	21.052476980066746	41.521205646087594	22.941079829040508
3	17.954531113055168	26.186639537631457	30.408332292596935	25.450497056716436
4	12.566666636280202	18.314630971079477	38.744939767444286	30.373762625196033
5	13.511584383666788	37.73408339281484	35.07446760129353	13.67986462222484
6	29.610318837595628	37.86215447371136	18.979963695635917	13.547562993057099
7	26.54030759407371	32.850087264860676	22.956953420690365	17.65265172037525
8	24.554673706602685	36.4796187349568	21.077125201449594	17.888582356990916
9	25.693892634695853	17.099142017926596	21.2046673203808	36.002298026996755
10-11	24.180221308670056	26.83254434879123	28.723271179846645	20.263963162692068
12-13	25.2342821709346	24.31198208170981	28.831365961710826	21.622369785644764
14-15	22.432685625591965	25.627012027063806	26.598887632577412	25.34141471476682
16-17	21.833515196321372	28.749823885118097	27.18963594686299	22.22702497169754
18-19	21.840747174793172	27.76931345937031	29.073606854051885	21.31633251178463
20-21	22.739435610363604	26.881898943877573	28.962829332378487	21.415836113380333
22-23	22.46646142300706	26.785629715632293	28.8051140691758	21.942794792184845
24-25	22.224844431044044	26.97642023471291	28.858249690473244	21.940485643769804
26-27	22.145313463750842	27.129579754144352	28.81903347846973	21.90607330363508
28-29	22.067037958868152	27.0698433134064	28.90759519634059	21.955523531384856
30-31	22.401334743400366	26.992914595960755	28.687066669943373	21.918683990695506
32-33	22.22169678732134	27.132880246689478	28.6711466298317	21.97427633615748
34-35	22.303589450391023	27.124420264244247	28.639621685029315	21.932368600335415
36-37	22.292917596720464	27.0822996552286	28.598974755477418	22.025807992573522
38-39	22.127200780400628	27.19606781152472	28.680409498700786	21.99632190937387
40-41	22.42757949008409	27.026290706712064	28.53097679474656	22.01515300845729
42-43	22.347045532638084	27.096565249276267	28.316531860528947	22.239857357556698
44-45	22.404033790020218	27.254681086800105	28.114783966202584	22.2265011569771
46-47	22.591539213559507	27.24762169954637	27.74186130294988	22.418977783944246
48-49	22.478508155240075	27.38277659670555	27.697063736109666	22.44165151194471
50-51	22.454650583880614	27.504712837107547	27.626462148705677	22.41417443030616
52-53	22.67588055371891	27.414140202847868	27.530249166492744	22.37973007694048
54-55	22.602755310081456	27.318471684406976	27.544297942215383	22.534475063296185
56-57	22.56955118213221	27.491687521753143	27.48853608233876	22.450225213775887
58-59	22.509783703481435	27.55988051017989	27.55701690504027	22.373318881298403
60-61	22.63874881321433	27.55187584756754	27.475319076673248	22.334056262544877
62-63	22.569430845255546	27.666899110505522	27.42788008364324	22.33578996059569
64-65	22.574419677220963	27.598399805110375	27.35707882921622	22.47010168845244
66-67	22.585095271018726	27.64229668019716	27.2724558772145	22.500152171569614
68-69	22.564851747584118	27.543482204281084	27.402245874568305	22.489420173566494
70-71	22.6052611859598	27.26484811400124	27.486677398852464	22.643213301186496
72-73	22.64489666817708	27.3534161378284	27.342432952851674	22.659254241142843
74-75	22.45690713472172	27.497903154027405	27.371821329688277	22.673368381562597
76-77	22.612539822077757	27.432605997856953	27.323769774127456	22.63108440593784
78-79	22.59709186464832	27.41726693399395	27.282987621733774	22.70265357962396
80-81	22.60136601080449	27.443036095162913	27.299846275137384	22.655751618895206
82-83	22.696166886672763	27.427130413229655	27.32301088513804	22.55369181495954
84-85	22.709379835936957	27.406515172715668	27.303462276484357	22.580642714863014
86-87	22.559191191067487	27.457085995504432	27.395047573428943	22.588675239999137
88-89	22.639816869027133	27.44205601398233	27.31028125712976	22.607845859860774
90-91	22.657760886674964	27.438968501876747	27.35357871971731	22.54969189173098
92-93	22.691188697676324	27.42703733029132	27.353463306178185	22.52831066585417
94-95	22.574658492398907	27.464558749411538	27.370029986817702	22.590752771371854
96-97	22.650343552499507	27.569786567843117	27.440719914223425	22.339149965433943
98-99	23.692692878118056	29.529532114787017	27.593099396664016	19.184675610430908
100	22.822144109892687	30.477987049336747	21.174476830231505	25.52539201053906
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1073.0
1	1028.0
2	2131.5
3	3378.5
4	4365.0
5	7621.0
6	10501.5
7	12180.0
8	12977.5
9	12172.0
10	10321.5
11	8117.5
12	8062.0
13	11123.0
14	20229.5
15	33768.5
16	45753.0
17	53636.5
18	56020.0
19	53168.5
20	47304.0
21	40843.5
22	37093.5
23	38789.0
24	46005.0
25	58611.0
26	76002.0
27	95002.5
28	113188.5
29	133114.5
30	153440.5
31	173946.5
32	197820.5
33	222099.5
34	246157.0
35	269182.0
36	293115.5
37	315771.0
38	332494.5
39	346244.0
40	361327.0
41	373857.5
42	381961.5
43	391440.5
44	403036.5
45	413304.5
46	415533.0
47	413823.0
48	413297.5
49	410233.0
50	403405.0
51	392969.5
52	378669.0
53	361413.0
54	343508.0
55	323168.0
56	300432.5
57	275574.5
58	248284.0
59	217948.0
60	183067.0
61	149409.5
62	119160.5
63	91718.5
64	68757.5
65	50946.5
66	37671.0
67	28509.0
68	21639.5
69	15664.5
70	11060.5
71	7753.5
72	5282.5
73	3499.5
74	2363.0
75	1615.0
76	1129.0
77	779.0
78	560.0
79	437.5
80	315.5
81	205.0
82	145.0
83	94.5
84	64.5
85	48.5
86	37.0
87	29.5
88	21.5
89	16.5
90	12.0
91	5.5
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	6.988886758460123E-4
3	0.0
4	0.0
5	0.0
6	0.04082927901935182
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.008627549838665325
26-27	0.004687484638986149
28-29	0.0017341429989528715
30-31	9.647235137951908E-4
32-33	0.0033289632631088363
34-35	0.003441745420423246
36-37	0.015223273307405898
38-39	0.0014710494373777869
40-41	0.002718593217936415
42-43	0.0024823341859679933
44-45	0.003804918650046571
46-47	0.005746020960587316
48-49	0.0014289496572947342
50-51	0.0
52-53	0.012571181437877924
54-55	0.004831958029226002
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0039506660012192255
64-65	1.1512854735519217E-5
66-67	2.9040367446607687E-5
68-69	0.006419421052640836
70-71	2.5451291348212315E-4
72-73	0.0022455599874176974
74-75	0.0
76-77	2.1881466999192027E-4
78-79	7.363432262682101E-4
80-81	0.002348639626408897
82-83	0.00844902235423559
84-85	0.005599150850224105
86-87	0.0
88-89	0.0
90-91	9.596465993682392E-4
92-93	6.609236368169097E-6
94-95	0.001011745019618138
96-97	0.0037239891316914616
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	227.0
20-21	829.0
22-23	1734.0
24-25	2395.0
26-27	4793.0
28-29	9921.0
30-31	23402.0
32-33	29232.0
34-35	38816.0
36-37	57296.0
38-39	68080.0
40-41	69624.0
42-43	81186.0
44-45	97840.0
46-47	93817.0
48-49	81078.0
50-51	71508.0
52-53	68756.0
54-55	73497.0
56-57	75057.0
58-59	75206.0
60-61	72862.0
62-63	71227.0
64-65	76155.0
66-67	79577.0
68-69	80677.0
70-71	78121.0
72-73	71453.0
74-75	73274.0
76-77	77021.0
78-79	79445.0
80-81	80256.0
82-83	77263.0
84-85	78074.0
86-87	82309.0
88-89	88310.0
90-91	93245.0
92-93	99152.0
94-95	121679.0
96-97	335313.0
98-99	643167.0
100-101	6389943.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.28833706302757
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.17590103845507	76.90952642729974
2	6.831940401122814	11.653696714509568
3	1.6442417105402607	4.207039236649403
4	0.6057718452336605	2.0666109327832247
5	0.27857325907434216	1.1879525008339298
6	0.15884981408495105	0.8128821891645942
7	0.08291441548122155	0.49501428304624345
8	0.056340832759190634	0.3844172747822013
9	0.03459216422329197	0.2655277345814156
>10	0.1275152349915173	1.709357376704232
>50	0.0025719284463290174	0.15478238806999484
>100	7.275764302634843E-4	0.09911558002070417
>500	3.9853061928322014E-5	0.026834961162291456
>1k	1.9926095277932047E-5	0.027242400392540757
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.0128821389072642E-5	0.0	0.0	0.0
3	0.0	1.0128821389072642E-5	0.0	0.0	0.0
4	0.0	4.051528555629057E-5	0.0	0.0	1.0128821389072642E-5
5	0.0	6.0772928334435854E-5	0.0	0.0	1.0128821389072642E-5
6	0.0	6.0772928334435854E-5	0.0	0.0	1.0128821389072642E-5
7	0.0	6.0772928334435854E-5	0.0	1.0128821389072642E-5	1.0128821389072642E-5
8	0.0	8.103057111258114E-5	0.0	1.0128821389072642E-5	2.0257642778145285E-5
9	0.0	1.8231878500330755E-4	0.0	1.0128821389072642E-5	4.051528555629057E-5
10-11	0.0	1.8231878500330755E-4	0.0	2.0257642778145285E-5	4.051528555629057E-5
12-13	0.0	1.8231878500330755E-4	0.0	2.0257642778145285E-5	4.051528555629057E-5
14-15	5.064410694536321E-6	2.937358202831067E-4	0.0	2.0257642778145285E-5	7.596616041804483E-5
16-17	1.0128821389072642E-5	3.089290523667156E-4	0.0	2.0257642778145285E-5	1.1141703527979907E-4
18-19	1.0128821389072642E-5	3.4944433792300617E-4	0.0	2.0257642778145285E-5	1.2154585666887171E-4
20-21	2.0257642778145285E-5	4.051528555629057E-4	0.0	2.5322053472681604E-5	1.2154585666887171E-4
22-23	2.0257642778145285E-5	4.25410498341051E-4	0.0	3.545087486175425E-5	1.671255529196986E-4
24-25	2.0257642778145285E-5	4.811190159809505E-4	0.0	7.596616041804481E-5	2.0257642778145286E-4
26-27	2.0257642778145285E-5	4.963122480645595E-4	0.0	1.2661026736340803E-4	2.0257642778145286E-4
28-29	2.0257642778145285E-5	5.013766587590959E-4	0.0	3.747663913956878E-4	2.0257642778145286E-4
30-31	2.0257642778145285E-5	5.469563550099227E-4	0.0	0.0011952009239105717	2.0764083847598917E-4
32-33	2.0257642778145285E-5	5.520207657044591E-4	0.0	0.0031753855054742733	2.1270524917052548E-4
34-35	2.0257642778145285E-5	6.634378009842581E-4	0.0	0.005844329941494915	2.2283407055959814E-4
36-37	2.0257642778145285E-5	7.647260148749846E-4	0.0	0.009440061534615702	2.2789848125413445E-4
38-39	2.0257642778145285E-5	8.001768897367388E-4	0.0	0.015284391476110618	2.329628919486708E-4
40-41	2.0257642778145285E-5	8.812074608493199E-4	0.0	0.021250267274274405	2.4309171333774341E-4
42-43	2.0257642778145285E-5	9.065295143220015E-4	0.0	0.026881891966598792	2.4815612403227975E-4
44-45	2.0257642778145285E-5	9.7743126404551E-4	0.0	0.03292373392518062	2.5322053472681607E-4
46-47	2.0257642778145285E-5	0.0010331397816854095	0.0	0.039760688362804664	2.582849454213524E-4
48-49	2.0257642778145285E-5	0.0010331397816854095	0.0	0.04662802926459591	3.038646416721793E-4
50-51	2.0257642778145285E-5	0.001104041531408918	0.0	0.053338373434856534	3.038646416721793E-4
52-53	2.5322053472681604E-5	0.001104041531408918	0.0	0.060099361712062524	3.5450874861754254E-4
54-55	3.0386464167217927E-5	0.0011242991741870633	0.0	0.0659386272428629	4.2034608764651466E-4
56-57	3.0386464167217927E-5	0.0011445568169652085	0.0	0.07026363397599691	4.4060373042465996E-4
58-59	3.0386464167217927E-5	0.0011749432811324265	0.0	0.07494821386844303	4.507325518137326E-4
60-61	3.0386464167217927E-5	0.0012053297452996445	0.0	0.07926309178018796	4.557969625082689E-4
62-63	3.0386464167217927E-5	0.0012559738522450077	0.0	0.08334500679998424	4.8618342667548683E-4
64-65	3.0386464167217927E-5	0.0014433570479428517	0.0	0.08760924060478381	4.8618342667548683E-4
66-67	3.0386464167217927E-5	0.001478807922804606	0.0	0.09167596239249648	4.963122480645595E-4
68-69	3.0386464167217927E-5	0.0015142587976663601	0.0	0.09588955209035072	5.165698908427048E-4
70-71	3.0386464167217927E-5	0.0015243876190554327	0.0	0.09970305334333655	5.266987122317774E-4
72-73	3.0386464167217927E-5	0.0015294520297499691	0.0	0.10263028272477856	5.773428191771406E-4
74-75	3.0386464167217927E-5	0.0015547740832226506	0.0	0.10488394548384722	5.773428191771406E-4
76-77	3.0386464167217927E-5	0.0016206114222516229	0.0	0.10730979820653011	5.773428191771406E-4
78-79	3.0386464167217927E-5	0.0016408690650297681	0.0	0.10880379936141832	5.773428191771406E-4
80-81	3.0386464167217927E-5	0.0016408690650297681	0.0	0.10933556248434464	5.874716405662132E-4
82-83	3.0386464167217927E-5	0.0016611267078079134	0.0	0.10968500682226764	6.229225154279676E-4
84-85	3.0386464167217927E-5	0.0016813843505860586	0.0	0.10976603739338023	6.279869261225039E-4
86-87	3.0386464167217927E-5	0.001686448761280595	0.0	0.10981161708963105	6.279869261225039E-4
88	3.0386464167217927E-5	0.0018231878500330756	0.0	0.10981668150032559	6.279869261225039E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13095	0.0	53.300156	1
GTATCAA	24570	0.0	39.023193	1
TCAACGC	29935	0.0	31.504002	4
ATCAACG	30340	0.0	31.097464	3
CAACGCA	31150	0.0	30.329742	5
TATCAAC	31355	0.0	30.267235	2
AACGCAG	31985	0.0	29.577799	6
ACGCAGA	36525	0.0	25.914478	7
CGCAGAG	37375	0.0	25.347843	8
GTGGTAT	5905	0.0	22.15783	1
GCAGAGT	43210	0.0	21.83647	9
GTACATG	31105	0.0	20.800133	1
TGGTATC	5835	0.0	20.603544	2
GAGTACT	24980	0.0	20.467117	12-13
TACATGG	31335	0.0	20.227188	2
ACATGGG	32065	0.0	19.475029	3
CAGAGTA	42080	0.0	19.395468	10-11
AGAGTAC	38915	0.0	19.07423	10-11
GTACTTT	27730	0.0	18.254313	14-15
AGTACTT	26780	0.0	17.2917	12-13
>>END_MODULE