FastQCFastQC Report
Fri 27 May 2016
SRR2140355_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140355_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8663506
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA107850.050.1542631
GTATCAA200000.039.9460531
TCAACGC239500.032.5506974
ATCAACG241000.032.34813
CAACGCA246150.031.7759485
TATCAAC249800.031.3293742
AACGCAG256100.030.6587266
ACGCAGA292650.026.7615577
CGCAGAG299850.026.0760238
GTGGTAT52050.023.753591
TGGTATC50800.022.2253972
GCAGAGT352650.022.1109859
GTACATG257400.022.031891
TACATGG261000.021.399042
ACATGGG266850.020.382593
GAGTACT198950.020.37300312-13
CAGAGTA338250.019.36671810-11
AGAGTAC312750.018.93558710-11
CATGGGG215900.018.9094034
GTACTTT217650.018.55466314-15
Produced by FastQC (version 0.11.5)