##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140355_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8663506
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.33341536324901	34.0	31.0	34.0	31.0	34.0
2	32.5039559042263	34.0	31.0	34.0	31.0	34.0
3	32.63218943924088	34.0	31.0	34.0	31.0	34.0
4	36.10263558425423	37.0	35.0	37.0	35.0	37.0
5	36.19147398293485	37.0	37.0	37.0	35.0	37.0
6	36.17137934688335	37.0	37.0	37.0	35.0	37.0
7	36.16049437721865	37.0	36.0	37.0	35.0	37.0
8	36.14912392280908	37.0	36.0	37.0	35.0	37.0
9	37.885301401072496	39.0	38.0	39.0	35.0	39.0
10-11	37.85460845759211	39.0	38.0	39.0	35.0	39.0
12-13	37.848373279824585	39.0	38.0	39.0	35.0	39.0
14-15	39.24734143428769	40.0	39.0	41.0	36.0	41.0
16-17	39.25054700718162	40.0	39.0	41.0	36.0	41.0
18-19	39.30523069990371	40.0	39.0	41.0	36.0	41.0
20-21	39.31902598344306	40.0	39.0	41.0	36.0	41.0
22-23	39.28788081085567	40.0	39.0	41.0	36.0	41.0
24-25	39.241104995705484	40.0	39.0	41.0	36.0	41.0
26-27	39.19899854964909	40.0	39.0	41.0	36.0	41.0
28-29	39.17856035436722	40.0	39.0	41.0	36.0	41.0
30-31	39.09816877344073	40.0	39.0	41.0	35.5	41.0
32-33	38.9953748870212	40.0	38.0	41.0	35.0	41.0
34-35	38.97371139635862	40.0	38.0	41.0	35.0	41.0
36-37	38.90659118719988	40.0	38.0	41.0	35.0	41.0
38-39	38.853758508497	40.0	38.0	41.0	35.0	41.0
40-41	38.822764118301855	40.0	38.0	41.0	35.0	41.0
42-43	38.7666503961929	40.0	38.0	41.0	35.0	41.0
44-45	38.69708247720462	40.0	38.0	41.0	35.0	41.0
46-47	38.56839507924158	40.0	38.0	41.0	34.5	41.0
48-49	38.48639254844436	40.0	38.0	41.0	34.0	41.0
50-51	38.41680684082232	40.0	38.0	41.0	34.0	41.0
52-53	38.29775306988758	40.0	37.5	41.0	34.0	41.0
54-55	38.197960246253416	40.0	37.0	41.0	34.0	41.0
56-57	38.051004758342465	40.0	37.0	41.0	34.0	41.0
58-59	37.866928027129774	39.5	36.5	41.0	33.0	41.0
60-61	37.69415725480469	39.0	36.0	41.0	33.0	41.0
62-63	37.47448840091215	39.0	35.5	41.0	33.0	41.0
64-65	37.200792261862404	39.0	35.0	40.5	33.0	41.0
66-67	36.943933924395324	38.0	35.0	40.0	32.0	41.0
68-69	36.62976048243283	37.0	35.0	40.0	32.0	41.0
70-71	36.3291621422362	37.0	35.0	39.5	32.0	41.0
72-73	36.00428859325761	36.0	35.0	39.0	32.0	41.0
74-75	35.69016050098073	36.0	35.0	39.0	31.0	40.5
76-77	35.36680204713784	35.5	34.0	37.5	31.0	39.5
78-79	35.058182001312844	35.0	34.0	37.0	31.0	39.0
80-81	34.750501217084896	35.0	34.0	37.0	31.0	39.0
82-83	34.48029765394324	35.0	34.0	36.0	31.0	38.5
84-85	34.27446716321303	35.0	34.0	36.0	31.0	37.0
86-87	34.096267044084215	35.0	34.0	35.5	31.0	37.0
88-89	33.95190447769426	35.0	34.0	35.0	31.0	36.5
90-91	33.82502313008089	35.0	34.0	35.0	31.0	36.0
92-93	33.752657705269485	35.0	34.0	35.0	31.0	36.0
94-95	33.69775553419335	35.0	34.0	35.0	31.0	36.0
96-97	33.69741690949634	35.0	34.0	35.0	31.0	35.5
98-99	33.780101853564716	35.0	34.0	35.0	31.0	35.0
100	32.934510755144565	34.0	32.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	22.0
20	78.0
21	247.0
22	786.0
23	2261.0
24	5543.0
25	12049.0
26	22750.0
27	37660.0
28	56508.0
29	81007.0
30	108460.0
31	141433.0
32	184290.0
33	246980.0
34	357703.0
35	541685.0
36	947062.0
37	1936737.0
38	2594660.0
39	1337589.0
40	47994.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.36332334738384	20.102623579876322	14.239073649859538	24.294979422880296
2	14.554677432535943	20.801177086021838	41.56592517419202	23.078220307250202
3	18.10390620148471	26.118363627843046	30.06721528212712	25.71051488854512
4	12.765547804780189	18.066265551152156	38.3932555711279	30.774931072939754
5	13.703332115196782	37.52753215615018	34.98672477401181	13.782410954641227
6	30.0895785453974	37.552632894703265	18.725676862056982	13.632111697842353
7	26.70992551976071	32.60404044274916	22.89448405760901	17.791549979881125
8	24.68257077446475	36.533292641570284	20.911176145084912	17.872960438880057
9	25.81346397174539	17.142401702036103	21.084997228604678	35.959137097613834
10-11	24.592659138228797	26.681599804975033	28.430100931424303	20.295640125371875
12-13	25.456760807922336	24.14061351143521	28.582400704749322	21.820224975893133
14-15	22.560144818968208	25.424158533508255	26.270051639601792	25.745645007921738
16-17	22.064265898817407	28.55229741862013	26.93912833903503	22.444308343527435
18-19	22.152348021690063	27.591849073573677	28.706536360683536	21.54926654405272
20-21	22.91389187358204	26.755291516103753	28.607751411475927	21.723065198838277
22-23	22.688791679569814	26.73529553521773	28.44720955797787	22.128703227234592
24-25	22.475583798905582	26.89402735362779	28.501393339200064	22.12899550826656
26-27	22.381394233634406	27.020685701244624	28.4563983799219	22.14152168519907
28-29	22.27801184836478	26.98779692835406	28.491910026003364	22.24228119727779
30-31	22.689896227070744	26.853238765622635	28.343392542998192	22.113472464308433
32-33	22.481399198887985	27.03163133857712	28.31184440932831	22.175125053206582
34-35	22.54215961367487	27.046851819976947	28.35623440393988	22.0547541624083
36-37	22.52884017054772	26.928518851459586	28.318892011821305	22.223748966171385
38-39	22.376956169891812	27.070277546777	28.39263192075553	22.16013436257566
40-41	22.602052107605516	26.849456227555386	28.31367374053457	22.234817924304533
42-43	22.52760892634163	26.943302852472538	28.072413471432423	22.45667474975341
44-45	22.545171974479754	27.050223228793385	27.971121753755334	22.433483042971528
46-47	22.782605379237967	27.061792677080714	27.564135534096383	22.591466409584932
48-49	22.62690195121587	27.202055675204097	27.51809080445455	22.652951569125488
50-51	22.603205313675726	27.298822017704023	27.502877756145967	22.595094912474288
52-53	22.800756550833533	27.21005052716576	27.40519319320822	22.583999728792488
54-55	22.691422743565155	27.111800178835903	27.434431737806086	22.76234533979286
56-57	22.7041188552957	27.21968849496601	27.41181943233701	22.66437321740128
58-59	22.65447492275712	27.313436746030234	27.46647436913524	22.565613962077403
60-61	22.749256753299843	27.337396263326895	27.391806886043273	22.521540097329982
62-63	22.70085919545915	27.490846757132253	27.286121724568602	22.52217232284
64-65	22.65885452851842	27.42450968283131	27.228698028438654	22.687937760211618
66-67	22.70408262590054	27.456726657313542	27.129254817270144	22.709935899515774
68-69	22.635074021479387	27.382334475517617	27.23636638952357	22.74622511347943
70-71	22.65540685791572	27.120724531411994	27.322451273175645	22.901417337496635
72-73	22.734126266772662	27.17064578104571	27.145428274210325	22.949799677971303
74-75	22.592976284475892	27.362912611817574	27.183210181701668	22.860900922004866
76-77	22.802264180626352	27.23189986534767	27.144538538123903	22.82129741590207
78-79	22.714088133982628	27.29141544787189	27.07352847080046	22.92096794734502
80-81	22.78688705166218	27.32421690846052	27.031706073962575	22.857189965914728
82-83	22.76679773838121	27.316612701107424	27.16879566916675	22.74779389134461
84-85	22.82815109303459	27.330488194734393	27.097873795235706	22.743486916995316
86-87	22.704287692945467	27.36272301941336	27.191973711319406	22.741015576321765
88-89	22.731530537571444	27.338894819745096	27.1455581336829	22.78401650900056
90-91	22.79619914515112	27.32232362804354	27.17332795490303	22.708149271902307
92-93	22.866587078841903	27.283378318882228	27.18673173123057	22.6633028710453
94-95	22.692847204833182	27.340997621479584	27.240844257653627	22.725310916033607
96-97	22.771812731846527	27.43944617330821	27.309702881811514	22.47903821303375
98-99	23.80757881517708	29.400255442855173	27.44444667382715	19.347719068140602
100	22.876477469349656	30.3430598053535	21.131521066921017	25.64894165837583
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1116.0
1	1013.0
2	1735.5
3	2656.5
4	3333.5
5	5418.5
6	7289.5
7	8633.5
8	9415.5
9	9171.5
10	8189.5
11	6814.0
12	7018.5
13	9350.0
14	16480.0
15	26817.5
16	35837.5
17	42035.5
18	43864.0
19	41472.5
20	36516.5
21	31000.5
22	27807.5
23	28863.5
24	34009.0
25	43348.0
26	56102.0
27	70992.5
28	87395.0
29	106071.0
30	125288.5
31	145413.0
32	167634.0
33	188710.5
34	209074.5
35	228008.0
36	247745.5
37	267580.5
38	282557.5
39	293816.5
40	305696.5
41	316354.5
42	324000.0
43	332992.5
44	341038.0
45	351364.5
46	359760.5
47	361883.5
48	361775.5
49	356793.0
50	350543.5
51	343904.5
52	333856.5
53	319887.0
54	305388.5
55	288984.0
56	270312.5
57	248115.0
58	223174.5
59	197516.5
60	166502.0
61	136117.0
62	108785.5
63	83796.0
64	63469.0
65	47195.0
66	34769.0
67	26561.5
68	20519.0
69	15217.5
70	11065.0
71	8048.5
72	5769.5
73	3961.0
74	2717.0
75	1884.5
76	1330.5
77	951.0
78	661.5
79	474.0
80	343.5
81	243.5
82	182.0
83	147.5
84	118.5
85	86.5
86	74.5
87	70.5
88	55.5
89	43.0
90	33.5
91	24.5
92	16.5
93	8.5
94	3.0
95	1.5
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	9.003283428210241E-4
3	0.0
4	0.0
5	0.0
6	0.03791767443803929
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.008324816080450284
26-27	0.004700444809906274
28-29	0.0016810743208734145
30-31	8.730638580298717E-4
32-33	0.003191279501525617
34-35	0.0031985685273860157
36-37	0.014041613027821379
38-39	0.0013049398703603684
40-41	0.0027067114027219986
42-43	0.0022077656647928674
44-45	0.00359543026180039
46-47	0.004000199227967564
48-49	0.00137101372451469
50-51	6.111831730026701E-6
52-53	0.009683286997308058
54-55	0.002693425737856241
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.003956622652430483
64-65	0.0
66-67	1.9467646815096226E-5
68-69	0.006719306913315439
70-71	1.9176137584687444E-4
72-73	0.0019551693677142415
74-75	0.0
76-77	1.6302530404049384E-4
78-79	5.280667476369013E-4
80-81	0.002438512255359895
82-83	0.008713711939101162
84-85	0.005831986615696753
86-87	0.0
88-89	0.0
90-91	0.0010084455119351717
92-93	1.4801527428821491E-5
94-95	8.031420116455593E-4
96-97	0.004082316034985065
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	192.0
20-21	704.0
22-23	1386.0
24-25	1836.0
26-27	3043.0
28-29	5456.0
30-31	13875.0
32-33	18295.0
34-35	24711.0
36-37	38373.0
38-39	46115.0
40-41	48766.0
42-43	57819.0
44-45	72196.0
46-47	72339.0
48-49	63092.0
50-51	55245.0
52-53	51320.0
54-55	55539.0
56-57	59501.0
58-59	61258.0
60-61	62143.0
62-63	61741.0
64-65	66188.0
66-67	69775.0
68-69	72574.0
70-71	70850.0
72-73	65081.0
74-75	66179.0
76-77	69129.0
78-79	72567.0
80-81	74235.0
82-83	71417.0
84-85	70725.0
86-87	75358.0
88-89	80966.0
90-91	85247.0
92-93	91374.0
94-95	112292.0
96-97	305468.0
98-99	574094.0
100-101	5695042.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.59313977596541
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.12120207792717	78.90470988087937
2	6.27437926692701	10.866364017368602
3	1.4031760641144473	3.6451626315045402
4	0.5321207994136177	1.8431204304528739
5	0.2431336085616173	1.0526851275205493
6	0.1398585281220822	0.7266473444721752
7	0.07771369427062841	0.4710630953338218
8	0.049672515925203435	0.34410392916280075
9	0.03838832331450096	0.299174890188378
>10	0.11688585478736319	1.5595952507000852
>50	0.0028256076240422115	0.1602532550933651
>100	6.011066919966117E-4	0.0899411321861814
>500	2.127616446360447E-5	0.014481027282685575
>1k	2.1276155926459188E-5	0.02269798785451262
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.3085342123616004E-5	0.0	1.1542671061808002E-5	0.0
3	0.0	2.3085342123616004E-5	0.0	1.1542671061808002E-5	0.0
4	0.0	6.925602637084801E-5	0.0	2.3085342123616004E-5	0.0
5	0.0	8.079869743265601E-5	0.0	2.3085342123616004E-5	0.0
6	1.1542671061808002E-5	8.079869743265601E-5	0.0	2.3085342123616004E-5	0.0
7	1.1542671061808002E-5	8.079869743265601E-5	0.0	2.3085342123616004E-5	1.1542671061808002E-5
8	2.3085342123616004E-5	1.1542671061808002E-4	0.0	2.3085342123616004E-5	2.3085342123616004E-5
9	2.3085342123616004E-5	1.3851205274169603E-4	0.0	4.617068424723201E-5	3.4628013185424006E-5
10-11	2.3085342123616004E-5	1.3851205274169603E-4	0.0	5.771335530904001E-5	3.4628013185424006E-5
12-13	2.3085342123616004E-5	1.3851205274169603E-4	0.0	6.925602637084801E-5	3.4628013185424006E-5
14-15	2.3085342123616004E-5	2.5393876335977605E-4	0.0	6.925602637084801E-5	4.617068424723201E-5
16-17	3.4628013185424006E-5	2.5393876335977605E-4	0.0	6.925602637084801E-5	8.079869743265601E-5
18-19	3.4628013185424006E-5	2.712527699524881E-4	0.0	9.234136849446402E-5	9.811270402536802E-5
20-21	3.4628013185424006E-5	3.0010944760700805E-4	0.0	9.811270402536802E-5	1.0388403955627202E-4
22-23	3.4628013185424006E-5	3.4050879632333605E-4	0.0	1.1542671061808002E-4	1.1542671061808002E-4
24-25	4.617068424723201E-5	3.635941384469521E-4	0.0	1.4428338827260004E-4	1.2696938167988802E-4
26-27	4.617068424723201E-5	3.6936547397785607E-4	0.0	1.5582605933440801E-4	1.32740717210792E-4
28-29	4.617068424723201E-5	3.6936547397785607E-4	0.0	2.9433811207610404E-4	1.3851205274169603E-4
30-31	4.617068424723201E-5	4.790208490650321E-4	0.0	0.0010157550534391042	1.3851205274169603E-4
32-33	4.617068424723201E-5	4.847921845959361E-4	0.0	0.003024179818193697	1.5005472380350403E-4
34-35	4.617068424723201E-5	5.540482109667841E-4	0.0	0.0056674514913477286	1.7314006592712003E-4
36-37	4.617068424723201E-5	6.348469083994402E-4	0.0	0.01063080004792517	1.7314006592712003E-4
38-39	5.771335530904001E-5	6.810175926466721E-4	0.0	0.026634713475121963	1.8468273698892803E-4
40-41	5.771335530904001E-5	7.271882768939041E-4	0.0	0.044375798897120865	1.8468273698892803E-4
42-43	5.771335530904001E-5	7.387309479557121E-4	0.0	0.054654547477660895	1.9045407251983205E-4
44-45	5.771335530904001E-5	8.079869743265601E-4	0.0	0.06771507978409665	2.0776807911254404E-4
46-47	5.771335530904001E-5	8.657003296356001E-4	0.0	0.08041201795208544	2.4239609229796804E-4
48-49	5.771335530904001E-5	8.887856717592162E-4	0.0	0.09225479846150045	2.5393876335977605E-4
50-51	5.771335530904001E-5	0.0010330690600318163	0.0	0.10420146301047174	2.654814344215841E-4
52-53	5.771335530904001E-5	0.0010446117310936242	0.0	0.14335997458765537	2.9433811207610404E-4
54-55	5.771335530904001E-5	0.0010503830666245282	0.0	0.19128514483628223	3.289661252615281E-4
56-57	5.771335530904001E-5	0.0010619257376863363	5.771335530904001E-6	0.237588569800725	3.347374607924321E-4
58-59	6.925602637084801E-5	0.0010792397442790482	1.1542671061808002E-5	0.261152932773406	3.347374607924321E-4
60-61	6.925602637084801E-5	0.001090782415340856	1.1542671061808002E-5	0.27641811525264715	3.347374607924321E-4
62-63	9.234136849446402E-5	0.0011023250864026642	1.1542671061808002E-5	0.28841672182139655	3.4628013185424006E-4
64-65	9.234136849446402E-5	0.0013389498431697284	1.1542671061808002E-5	0.29383600588491543	3.4628013185424006E-4
66-67	9.234136849446402E-5	0.0013562638497624402	1.1542671061808002E-5	0.29879935444149286	3.4628013185424006E-4
68-69	9.234136849446402E-5	0.0013908918629478642	1.1542671061808002E-5	0.30361841960979774	3.520514673851441E-4
70-71	9.811270402536802E-5	0.0014082058695405763	1.1542671061808002E-5	0.30772761050780134	3.6936547397785607E-4
72-73	1.2696938167988802E-4	0.0014139772050714803	1.1542671061808002E-5	0.31087298837214405	3.9245081610147207E-4
74-75	1.2696938167988802E-4	0.0014428338827260003	1.1542671061808002E-5	0.3136778574401634	3.9245081610147207E-4
76-77	1.2696938167988802E-4	0.0014832332314423282	1.1542671061808002E-5	0.31627495842907016	3.982221516323761E-4
78-79	1.2696938167988802E-4	0.0015120899090968482	1.1542671061808002E-5	0.3178736183711306	4.039934871632801E-4
80-81	1.2696938167988802E-4	0.0015236325801586564	1.1542671061808002E-5	0.3184161239110356	4.097648226941841E-4
82-83	1.3851205274169603E-4	0.0015351752512204643	1.1542671061808002E-5	0.3187912607205443	4.155361582250881E-4
84-85	1.3851205274169603E-4	0.0015467179222822722	1.1542671061808002E-5	0.3189182301022242	4.155361582250881E-4
86-87	1.3851205274169603E-4	0.0015928886065295043	1.1542671061808002E-5	0.31899902879965686	4.155361582250881E-4
88	1.3851205274169603E-4	0.0016390592907767362	1.1542671061808002E-5	0.3190048001351878	4.270788292868961E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10785	0.0	50.154263	1
GTATCAA	20000	0.0	39.946053	1
TCAACGC	23950	0.0	32.550697	4
ATCAACG	24100	0.0	32.3481	3
CAACGCA	24615	0.0	31.775948	5
TATCAAC	24980	0.0	31.329374	2
AACGCAG	25610	0.0	30.658726	6
ACGCAGA	29265	0.0	26.761557	7
CGCAGAG	29985	0.0	26.076023	8
GTGGTAT	5205	0.0	23.75359	1
TGGTATC	5080	0.0	22.225397	2
GCAGAGT	35265	0.0	22.110985	9
GTACATG	25740	0.0	22.03189	1
TACATGG	26100	0.0	21.39904	2
ACATGGG	26685	0.0	20.38259	3
GAGTACT	19895	0.0	20.373003	12-13
CAGAGTA	33825	0.0	19.366718	10-11
AGAGTAC	31275	0.0	18.935587	10-11
CATGGGG	21590	0.0	18.909403	4
GTACTTT	21765	0.0	18.554663	14-15
>>END_MODULE