FastQCFastQC Report
Fri 27 May 2016
SRR2140354_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140354_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8757055
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA110100.051.624941
GTATCAA209100.040.182261
ATCAACG259150.031.6308863
TCAACGC260050.031.48864
CAACGCA265400.030.8865
TATCAAC268250.030.8288062
AACGCAG274900.029.9117766
ACGCAGA312650.026.2890557
CGCAGAG319050.025.7750748
GTGGTAT51350.022.4370611
CTTATAC82550.022.2276731
GCAGAGT377050.021.6857689
GTACATG275900.021.3282491
TGGTATC50900.021.2940652
TACATGG276800.021.0584932
GAGTACT212950.020.43027512-13
ACATGGG284800.019.8822773
TATACAC107650.019.5419583
AGAGTAC335200.019.34791210-11
CAGAGTA363150.019.01570710-11
Produced by FastQC (version 0.11.5)