##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140354_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8757055
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.32771770875026	34.0	31.0	34.0	31.0	34.0
2	32.49539508430631	34.0	31.0	34.0	31.0	34.0
3	32.62266709527347	34.0	31.0	34.0	31.0	34.0
4	36.09599996802578	37.0	35.0	37.0	35.0	37.0
5	36.184894693478576	37.0	37.0	37.0	35.0	37.0
6	36.16057921298884	37.0	37.0	37.0	35.0	37.0
7	36.15077032175771	37.0	36.0	37.0	35.0	37.0
8	36.14140061927212	37.0	36.0	37.0	35.0	37.0
9	37.8762997377543	39.0	38.0	39.0	35.0	39.0
10-11	37.843488307427556	39.0	38.0	39.0	35.0	39.0
12-13	37.83693764627492	39.0	38.0	39.0	35.0	39.0
14-15	39.22929066906626	40.0	39.0	41.0	36.0	41.0
16-17	39.23277157674583	40.0	39.0	41.0	36.0	41.0
18-19	39.2901972181287	40.0	39.0	41.0	36.0	41.0
20-21	39.30254153486935	40.0	39.0	41.0	36.0	41.0
22-23	39.27069965015225	40.0	39.0	41.0	36.0	41.0
24-25	39.22256987757104	40.0	39.0	41.0	36.0	41.0
26-27	39.1770856877484	40.0	39.0	41.0	36.0	41.0
28-29	39.15806842694809	40.0	39.0	41.0	36.0	41.0
30-31	39.0777485638349	40.0	39.0	41.0	35.5	41.0
32-33	38.973061991022504	40.0	38.0	41.0	35.0	41.0
34-35	38.94971229811957	40.0	38.0	41.0	35.0	41.0
36-37	38.8820767204723	40.0	38.0	41.0	35.0	41.0
38-39	38.82637573202307	40.0	38.0	41.0	35.0	41.0
40-41	38.79514840457847	40.0	38.0	41.0	35.0	41.0
42-43	38.74053610996852	40.0	38.0	41.0	35.0	41.0
44-45	38.67118428478619	40.0	38.0	41.0	35.0	41.0
46-47	38.5399633052365	40.0	38.0	41.0	34.0	41.0
48-49	38.458689633123	40.0	38.0	41.0	34.0	41.0
50-51	38.388007917256346	40.0	38.0	41.0	34.0	41.0
52-53	38.27239920541019	40.0	37.5	41.0	34.0	41.0
54-55	38.17010044315022	40.0	37.0	41.0	34.0	41.0
56-57	38.02527721893202	40.0	37.0	41.0	34.0	41.0
58-59	37.84384297898454	39.5	36.5	41.0	33.0	41.0
60-61	37.663651406949896	39.0	36.0	41.0	33.0	41.0
62-63	37.44259457347164	39.0	35.5	41.0	33.0	41.0
64-65	37.17189785544281	39.0	35.0	40.0	33.0	41.0
66-67	36.917406630089204	38.0	35.0	40.0	32.0	41.0
68-69	36.6043006337556	37.0	35.0	40.0	32.0	41.0
70-71	36.3073427286035	37.0	35.0	39.5	32.0	41.0
72-73	35.98296324787037	36.0	35.0	39.0	31.5	41.0
74-75	35.672705534703326	36.0	35.0	39.0	31.0	40.0
76-77	35.350758012008185	35.5	34.0	37.5	31.0	39.5
78-79	35.04366477595153	35.0	34.0	37.0	31.0	39.0
80-81	34.73683204155283	35.0	34.0	37.0	31.0	39.0
82-83	34.466382646624815	35.0	34.0	36.0	31.0	38.5
84-85	34.26004431631043	35.0	34.0	36.0	31.0	37.0
86-87	34.080988956793405	35.0	34.0	35.5	31.0	37.0
88-89	33.93833863073294	35.0	34.0	35.0	31.0	36.5
90-91	33.81308754204963	35.0	34.0	35.0	31.0	36.0
92-93	33.739307229894024	35.0	34.0	35.0	31.0	36.0
94-95	33.68368931305945	35.0	34.0	35.0	31.0	36.0
96-97	33.68697073724613	35.0	34.0	35.0	31.0	35.5
98-99	33.767516048209814	35.0	34.0	35.0	31.0	35.0
100	32.91937142799906	34.0	32.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	4.0
19	26.0
20	71.0
21	229.0
22	800.0
23	2328.0
24	5880.0
25	12428.0
26	23146.0
27	38623.0
28	58320.0
29	81935.0
30	109868.0
31	144345.0
32	187976.0
33	251646.0
34	364743.0
35	551775.0
36	961003.0
37	1956444.0
38	2603870.0
39	1353660.0
40	47935.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.25488534672901	20.171233365554972	14.258789056366552	24.315092231349468
2	14.502059156440161	20.879620198004247	41.621715878434856	22.996604767120736
3	17.933369152072242	26.296271977280032	30.283811167110404	25.486547703537322
4	12.7046821105954	18.245060696775344	38.407318442101825	30.64293875052743
5	13.564274747617777	37.66939913018703	35.038526079829346	13.727800042365839
6	29.872758618601786	37.732057953040446	18.75867595117442	13.63650747718334
7	26.564387228354736	32.71090566406172	22.87480208814493	17.849905019438612
8	24.63749513963313	36.558123707113864	20.95831304017161	17.846068113081394
9	25.775651745935136	17.122194619081416	21.007724628884937	36.09442900609851
10-11	24.507919614527943	26.698490531348725	28.534467352323357	20.25912250179998
12-13	25.43785553476597	24.177020699310443	28.61367777180799	21.771445994115602
14-15	22.573188132311607	25.39004265703481	26.29001987540332	25.746749335250264
16-17	22.026651654009253	28.57846045274353	26.930863172607687	22.46402472063953
18-19	22.141421973483094	27.593608810267835	28.72659244460609	21.538376771642977
20-21	22.920911732512174	26.71467733121991	28.591524124002838	21.772886812265074
22-23	22.674679196437694	26.703906039737262	28.462246081491365	22.15916868233368
24-25	22.46649302591468	26.86589790488128	28.56642649834264	22.101182570861404
26-27	22.330945086783473	26.95796281575194	28.55004719703543	22.161044900429168
28-29	22.15000677877131	26.864596500782245	28.607723821196817	22.377672899249625
30-31	22.483485866331993	26.70973783639375	28.55884822696301	22.247928070311243
32-33	22.302003899804838	26.940487703197487	28.483050674015676	22.274457722982003
34-35	22.421703293229164	27.055954614776727	28.517577829960384	22.00476426203372
36-37	22.435098537695577	26.90658026891675	28.374672625410764	22.283648567976915
38-39	22.349049815969625	27.000293012252392	28.466671118024188	22.183986053753795
40-41	22.560912966000068	26.796066247753824	28.40130665877158	22.24171412747453
42-43	22.539041461406	26.85986623957995	28.16846999937898	22.432622299635067
44-45	22.494572475594868	27.029275852554687	28.048908392318676	22.427243279531766
46-47	22.79530414072918	26.991971423017937	27.593646851786563	22.61907758446633
48-49	22.64267830632835	27.139838236761637	27.53836383563905	22.679119621270967
50-51	22.54813228360861	27.223885012109168	27.586776893681986	22.641205810600237
52-53	22.775036778074092	27.11927220087248	27.551071538132078	22.55461948292135
54-55	22.658073352285157	27.052767079758226	27.52780458750789	22.761354980448722
56-57	22.716339953452113	27.222115801879493	27.396791569619396	22.664752675048998
58-59	22.575921823281465	27.40680029398712	27.513236562154898	22.50404132057652
60-61	22.73504174164109	27.466929661977414	27.352407189105598	22.445621407275897
62-63	22.624613305291533	27.639023793706773	27.242587276605583	22.49377562439611
64-65	22.623478141386556	27.529726698035024	27.227948591728936	22.61884656884948
66-67	22.68135727654524	27.529441394221593	27.112739712241563	22.67646161699161
68-69	22.59901248686356	27.428309998038337	27.25404605621889	22.71863145887922
70-71	22.660252664187844	27.09777947615391	27.345068365301646	22.8968994943566
72-73	22.725947463431645	27.147568189440573	27.225034173221825	22.901450173905953
74-75	22.534143463642028	27.298218385935797	27.28812916201548	22.879508988406695
76-77	22.758603568784512	27.208684897024472	27.195864179288726	22.836847354902293
78-79	22.690893182506528	27.268682742436283	27.134856115429073	22.90556795962812
80-81	22.731659016199636	27.33797950937985	27.08464443973751	22.845717034683002
82-83	22.735092303787553	27.26480606051558	27.22848356526249	22.77161807043438
84-85	22.817571016845566	27.268899909575246	27.155538008164914	22.75799106541427
86-87	22.64500351005271	27.319538378006648	27.254336359740638	22.781121752200004
88-89	22.699473627429796	27.296665130846925	27.202556402329243	22.80130483939404
90-91	22.726519004283634	27.242300280604482	27.265094912750342	22.766085802361538
92-93	22.766280805246492	27.225281710803067	27.277514813027654	22.730922670922784
94-95	22.58990279551945	27.316577012145977	27.316471789716278	22.777048402618295
96-97	22.68076890840493	27.435356268915346	27.333643695733706	22.55023112694602
98-99	23.721693406082878	29.403632893961078	27.48928652529255	19.38538717466349
100	22.85882030635364	30.290304401300876	21.124648609912498	25.726226682432983
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1428.0
1	1242.5
2	1891.5
3	2824.0
4	3581.0
5	5818.5
6	7859.5
7	9347.0
8	10419.5
9	10356.5
10	9095.0
11	7439.5
12	7576.0
13	9996.0
14	17436.5
15	28362.5
16	37783.0
17	43966.5
18	45791.0
19	43214.0
20	38017.5
21	32617.5
22	29469.0
23	30525.5
24	35978.5
25	45613.0
26	58475.0
27	73413.0
28	90064.5
29	108971.5
30	127608.5
31	147345.5
32	169398.5
33	190064.0
34	210716.5
35	230316.0
36	251016.0
37	271627.5
38	287522.5
39	299291.5
40	311853.5
41	322726.5
42	329647.5
43	339056.5
44	354022.0
45	370862.5
46	374219.5
47	371551.5
48	368578.5
49	360465.5
50	354657.0
51	348946.5
52	338770.5
53	324446.0
54	309629.5
55	292712.0
56	273906.5
57	251855.5
58	226787.5
59	200601.5
60	168777.0
61	138126.5
62	110755.0
63	85497.5
64	64662.0
65	47604.0
66	35041.5
67	26780.0
68	20770.0
69	15360.0
70	11122.0
71	8049.0
72	5786.0
73	4050.0
74	2727.0
75	1897.5
76	1353.5
77	915.0
78	630.5
79	441.0
80	324.0
81	245.0
82	200.0
83	170.5
84	137.5
85	116.5
86	109.5
87	97.0
88	76.0
89	53.5
90	34.5
91	19.0
92	12.0
93	7.5
94	3.0
95	2.0
96	2.0
97	1.0
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	8.107748552452851E-4
3	0.0
4	0.0
5	0.0
6	0.04216029247275482
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.008310257851023293
26-27	0.004878864757868912
28-29	0.0018404082551340816
30-31	0.0010241040919995615
32-33	0.0032274707635863336
34-35	0.0035579524058627007
36-37	0.016062830592699407
38-39	0.00142124295295535
40-41	0.002947728944124076
42-43	0.002385311603653804
44-45	0.0039736208426658145
46-47	0.0059844690771542145
48-49	0.001447978302527796
50-51	1.1555116109759737E-4
52-53	0.013192791517512787
54-55	0.004681502980803617
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0043818703172741605
64-65	2.5695264060914218E-5
66-67	1.2968632897297636E-5
68-69	0.007086379892704873
70-71	2.3163631933065314E-4
72-73	0.002184633315978749
74-75	0.0
76-77	2.4485130418346816E-4
78-79	9.408845014826485E-4
80-81	0.0026016206917508227
82-83	0.009062279093805732
84-85	0.005896764849335181
86-87	0.0
88-89	0.0
90-91	0.0010098322536909368
92-93	1.4822103996180048E-5
94-95	0.0010071188269440662
96-97	0.003864683031196657
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	190.0
20-21	724.0
22-23	1514.0
24-25	1923.0
26-27	3365.0
28-29	6272.0
30-31	16185.0
32-33	21593.0
34-35	29277.0
36-37	43959.0
38-39	52724.0
40-41	54372.0
42-43	63057.0
44-45	78199.0
46-47	78642.0
48-49	68192.0
50-51	59571.0
52-53	56126.0
54-55	60830.0
56-57	63123.0
58-59	65028.0
60-61	65140.0
62-63	66318.0
64-65	71002.0
66-67	76219.0
68-69	78220.0
70-71	74764.0
72-73	65497.0
74-75	66403.0
76-77	69969.0
78-79	73023.0
80-81	73762.0
82-83	71053.0
84-85	71058.0
86-87	75138.0
88-89	80663.0
90-91	85172.0
92-93	90910.0
94-95	110941.0
96-97	304071.0
98-99	572253.0
100-101	5690613.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.81485437854707
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.30353598018452	79.26503180366153
2	6.1103699417556685	10.60941753385139
3	1.416265821196991	3.6885873308559045
4	0.5177421055400518	1.7979082199240777
5	0.23863802702582806	1.0358662782715522
6	0.13105621537516737	0.6826575751919195
7	0.07539567978078333	0.458182547265819
8	0.057123083200074636	0.39673057197344863
9	0.0340190537766201	0.26580232797419123
>10	0.1125240458668787	1.5295830582292254
>50	0.002723912410776473	0.15643918249504177
>100	5.852647346135592E-4	0.09708921317875753
>500	2.086915209244564E-5	0.01670435712716484
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1419364158384296E-5
2	0.0	2.2838728316768593E-5	0.0	0.0	1.1419364158384296E-5
3	0.0	2.2838728316768593E-5	0.0	0.0	1.1419364158384296E-5
4	0.0	9.135491326707437E-5	0.0	0.0	1.1419364158384296E-5
5	0.0	9.135491326707437E-5	0.0	0.0	1.1419364158384296E-5
6	1.1419364158384296E-5	9.135491326707437E-5	0.0	0.0	2.2838728316768593E-5
7	1.1419364158384296E-5	9.135491326707437E-5	0.0	0.0	2.2838728316768593E-5
8	1.1419364158384296E-5	1.0277427742545868E-4	0.0	0.0	2.2838728316768593E-5
9	1.1419364158384296E-5	2.3980664732607023E-4	0.0	0.0	4.5677456633537186E-5
10-11	2.2838728316768593E-5	2.3980664732607023E-4	0.0	0.0	4.5677456633537186E-5
12-13	2.2838728316768593E-5	2.3980664732607023E-4	0.0	0.0	4.5677456633537186E-5
14-15	2.2838728316768593E-5	2.7406473980122313E-4	0.0	1.1419364158384296E-5	6.280650287111363E-5
16-17	3.425809247515289E-5	2.7977442188041525E-4	0.0	2.2838728316768593E-5	7.993554910869008E-5
18-19	3.425809247515289E-5	3.254518785139525E-4	0.0	5.138713871272933E-5	8.564523118788223E-5
20-21	3.425809247515289E-5	4.0538742762264254E-4	0.0	5.709682079192149E-5	9.135491326707437E-5
22-23	3.996777455434504E-5	5.310004333648698E-4	0.0	7.993554910869008E-5	1.0277427742545867E-4
24-25	5.138713871272933E-5	5.880972541567912E-4	5.709682079192148E-6	8.564523118788223E-5	1.1419364158384297E-4
26-27	5.709682079192149E-5	6.16645664552752E-4	1.1419364158384296E-5	1.7129046237576446E-4	1.1419364158384297E-4
28-29	5.709682079192149E-5	6.16645664552752E-4	1.1419364158384296E-5	5.424197975232541E-4	1.1419364158384297E-4
30-31	5.709682079192149E-5	7.080005778198265E-4	1.1419364158384296E-5	0.0016786465312824917	1.1419364158384297E-4
32-33	5.709682079192149E-5	7.194199419782107E-4	1.1419364158384296E-5	0.004356487426423609	1.3703236990061156E-4
34-35	7.993554910869008E-5	7.479683523741714E-4	1.1419364158384296E-5	0.007936458090077087	1.7129046237576446E-4
36-37	7.993554910869008E-5	7.822264448493243E-4	1.1419364158384296E-5	0.013503398117289431	1.7129046237576446E-4
38-39	7.993554910869008E-5	7.879361269285165E-4	1.1419364158384296E-5	0.028628345945069432	1.7129046237576446E-4
40-41	7.993554910869008E-5	8.850007222747831E-4	1.1419364158384296E-5	0.04467826226967857	1.7129046237576446E-4
42-43	7.993554910869008E-5	9.078394505915516E-4	1.1419364158384296E-5	0.05441327021470117	1.7129046237576446E-4
44-45	7.993554910869008E-5	0.001039162138412971	1.1419364158384296E-5	0.06702024824555744	1.8270982653414874E-4
46-47	7.993554910869008E-5	0.0011419364158384297	1.1419364158384296E-5	0.07894206442691065	1.884195086133409E-4
48-49	7.993554910869008E-5	0.001153355779996814	1.1419364158384296E-5	0.0903271704928198	1.9412919069253305E-4
50-51	9.706459534626653E-5	0.001227581647026312	1.1419364158384296E-5	0.10160379259922428	2.0554855485091736E-4
52-53	1.1419364158384297E-4	0.001233291329105504	1.1419364158384296E-5	0.12941565400696925	2.226776010884938E-4
54-55	1.1419364158384297E-4	0.0012390010111846962	1.1419364158384296E-5	0.16009948550054784	2.455163294052624E-4
56-57	1.1419364158384297E-4	0.0012561300574222727	1.1419364158384296E-5	0.19274173794728935	2.512260114844545E-4
58-59	1.2561300574222726E-4	0.0012789687857390412	1.1419364158384296E-5	0.2172134353387069	2.512260114844545E-4
60-61	1.313226878214194E-4	0.0013189365602933865	1.1419364158384296E-5	0.22750228244541115	2.5693569356364664E-4
62-63	1.7129046237576446E-4	0.001347484970689347	1.1419364158384296E-5	0.24104564833725495	2.7406473980122313E-4
64-65	1.7129046237576446E-4	0.00146167861227319	1.1419364158384296E-5	0.24676674978060548	2.7406473980122313E-4
66-67	1.770001444549566E-4	0.0014730979764315743	1.1419364158384296E-5	0.2517398828715818	2.7406473980122313E-4
68-69	1.9412919069253305E-4	0.0015130657509859194	1.1419364158384296E-5	0.25685575801453797	2.7406473980122313E-4
70-71	1.9412919069253305E-4	0.001530194797223496	1.1419364158384296E-5	0.26162334255066344	2.911937860387996E-4
72-73	2.0554855485091736E-4	0.001535904479302688	1.1419364158384296E-5	0.26583708792510724	3.197421964347603E-4
74-75	2.0554855485091736E-4	0.0015701625717778407	1.1419364158384296E-5	0.26874331610341606	3.197421964347603E-4
76-77	2.0554855485091736E-4	0.0016329690746489543	1.1419364158384296E-5	0.2715524796863786	3.254518785139525E-4
78-79	2.1696791900930164E-4	0.001655807802965723	1.1419364158384296E-5	0.27347093286498714	3.311615605931446E-4
80-81	2.1696791900930164E-4	0.001655807802965723	1.1419364158384296E-5	0.27398480425211447	3.3687124267233674E-4
82-83	2.1696791900930164E-4	0.0016615174850449152	1.1419364158384296E-5	0.27436735295142034	3.768390172266818E-4
84-85	2.1696791900930164E-4	0.0016729368492032996	1.1419364158384296E-5	0.2744644175467666	3.768390172266818E-4
86-87	2.1696791900930164E-4	0.0017243239879160288	1.1419364158384296E-5	0.2745215143675585	3.768390172266818E-4
88	2.1696791900930164E-4	0.0017814208087079504	1.1419364158384296E-5	0.2745215143675585	3.768390172266818E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11010	0.0	51.62494	1
GTATCAA	20910	0.0	40.18226	1
ATCAACG	25915	0.0	31.630886	3
TCAACGC	26005	0.0	31.4886	4
CAACGCA	26540	0.0	30.886	5
TATCAAC	26825	0.0	30.828806	2
AACGCAG	27490	0.0	29.911776	6
ACGCAGA	31265	0.0	26.289055	7
CGCAGAG	31905	0.0	25.775074	8
GTGGTAT	5135	0.0	22.437061	1
CTTATAC	8255	0.0	22.227673	1
GCAGAGT	37705	0.0	21.685768	9
GTACATG	27590	0.0	21.328249	1
TGGTATC	5090	0.0	21.294065	2
TACATGG	27680	0.0	21.058493	2
GAGTACT	21295	0.0	20.430275	12-13
ACATGGG	28480	0.0	19.882277	3
TATACAC	10765	0.0	19.541958	3
AGAGTAC	33520	0.0	19.347912	10-11
CAGAGTA	36315	0.0	19.015707	10-11
>>END_MODULE