FastQCFastQC Report
Fri 27 May 2016
SRR2140353_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140353_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11091182
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA124500.049.5375821
GTATCAA220000.036.0203671
TCAACGC263550.030.4046614
ATCAACG265000.030.238573
CAACGCA272000.029.4601045
TATCAAC278250.029.0537762
AACGCAG282000.028.5088396
ACGCAGA328050.024.4933957
CGCAGAG333000.024.102678
GTGGTAT59200.020.760961
GCAGAGT407400.019.7445439
TACATGG282450.019.6676332
GTACATG280600.019.591741
GAGTACT240500.019.3231112-13
ACATGGG290200.018.7754483
AGAGTAC357750.018.30761110-11
TGGTATC61350.018.2976512
CAGAGTA395600.018.25417910-11
CATGGGG230550.017.7247434
GTACTTT273050.016.97921414-15
Produced by FastQC (version 0.11.5)