FastQCFastQC Report
Fri 27 May 2016
SRR2140352_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140352_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14928437
Sequences flagged as poor quality0
Sequence length19-101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA188150.053.714741
GTATCAA338150.038.121831
TATCTCG146450.034.585194-95
TCAACGC419150.031.145214
ATCAACG427100.030.6591683
TATCAAC435000.030.2448432
CAACGCA435400.030.013315
AACGCAG452350.028.9495096
CCGTCTT76300.027.83400592-93
TATGCCG75500.027.23229888-89
TGCCGTC79750.026.24112590-91
TCTCGTA72450.025.54721882-83
GCCGTCT79400.025.07532390-91
ACGCAGA527950.024.7621737
ATGCCGT80050.024.59869488-89
CGCAGAG541500.024.1262058
CTCGTAT85250.023.68114584-85
CGTATGC88400.023.25895186-87
TCGTATG87300.022.36336784-85
CTATCTC233000.021.84779594-95
Produced by FastQC (version 0.11.5)