##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140352_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	14928437
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.11530282775082	28.0	18.0	32.0	18.0	33.0
2	27.684325291388507	29.0	25.0	31.0	18.0	33.0
3	30.467965065599298	31.0	29.0	33.0	27.0	33.0
4	30.241108027585206	31.0	29.0	33.0	27.0	33.0
5	31.969998131753513	33.0	32.0	33.0	31.0	33.0
6	35.81150216864632	38.0	36.0	38.0	31.0	38.0
7	36.08418731311255	38.0	37.0	38.0	33.0	38.0
8	36.529030936058476	38.0	37.0	38.0	34.0	38.0
9	36.71809332751982	38.0	38.0	38.0	34.0	38.0
10-11	36.86552520535137	38.0	38.0	38.0	35.0	38.0
12-13	36.93024366850997	38.0	38.0	38.0	35.0	38.0
14-15	36.947946861416234	38.0	38.0	38.0	35.0	38.0
16-17	36.975383658717924	38.0	38.0	38.0	35.0	38.0
18-19	36.98397350640258	38.0	38.0	38.0	35.0	38.0
20-21	36.993169925732005	38.0	38.0	38.0	36.0	38.0
22-23	37.00799642344965	38.0	38.0	38.0	36.0	38.0
24-25	37.0241220401417	38.0	38.0	38.0	36.0	38.0
26-27	36.989082617447394	38.0	38.0	38.0	36.0	38.0
28-29	36.971763093409464	38.0	38.0	38.0	36.0	38.0
30-31	36.961307259681206	38.0	38.0	38.0	36.0	38.0
32-33	36.93941079136351	38.0	38.0	38.0	35.0	38.0
34-35	36.931062180055164	38.0	38.0	38.0	35.0	38.0
36-37	36.912525484836536	38.0	38.0	38.0	35.0	38.0
38-39	36.8966900616052	38.0	38.0	38.0	35.0	38.0
40-41	36.87880017836157	38.0	38.0	38.0	35.0	38.0
42-43	36.84012053674144	38.0	38.0	38.0	35.0	38.0
44-45	36.80574266620385	38.0	38.0	38.0	35.0	38.0
46-47	36.75805706922447	38.0	38.0	38.0	35.0	38.0
48-49	36.72219098602703	38.0	38.0	38.0	35.0	38.0
50-51	36.692311058198044	38.0	38.0	38.0	35.0	38.0
52-53	36.5950821585731	38.0	38.0	38.0	34.0	38.0
54-55	36.63797102072989	38.0	38.0	38.0	34.0	38.0
56-57	36.63251950092608	38.0	38.0	38.0	34.0	38.0
58-59	36.625749702515165	38.0	38.0	38.0	34.0	38.0
60-61	36.594426848723494	38.0	38.0	38.0	34.0	38.0
62-63	36.60931052457032	38.0	38.0	38.0	34.0	38.0
64-65	36.62225621088496	38.0	38.0	38.0	34.0	38.0
66-67	36.61778447398288	38.0	38.0	38.0	34.0	38.0
68-69	36.6057036804918	38.0	38.0	38.0	34.0	38.0
70-71	36.6036252421393	38.0	38.0	38.0	34.0	38.0
72-73	36.59381875671103	38.0	38.0	38.0	34.0	38.0
74-75	36.58278075505967	38.0	38.0	38.0	34.0	38.0
76-77	36.56497337081806	38.0	38.0	38.0	34.0	38.0
78-79	36.54750522258128	38.0	38.0	38.0	34.0	38.0
80-81	36.52539974283842	38.0	38.0	38.0	34.0	38.0
82-83	36.497331778416886	38.0	38.0	38.0	34.0	38.0
84-85	36.5001091563913	38.0	38.0	38.0	34.0	38.0
86-87	36.48810776706814	38.0	38.0	38.0	34.0	38.0
88-89	36.46353103036784	38.0	38.0	38.0	34.0	38.0
90-91	36.45707448322667	38.0	38.0	38.0	34.0	38.0
92-93	36.4641610339192	38.0	38.0	38.0	34.0	38.0
94-95	36.44266143399328	38.0	38.0	38.0	34.0	38.0
96-97	36.39007580786983	38.0	38.0	38.0	34.0	38.0
98-99	36.39368970618098	38.0	38.0	38.0	34.0	38.0
100-101	35.90189914329521	38.0	36.5	38.0	31.5	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	12.0
19	19.0
20	83.0
21	489.0
22	2173.0
23	7007.0
24	17156.0
25	33122.0
26	55831.0
27	86764.0
28	125017.0
29	168676.0
30	219329.0
31	280924.0
32	358381.0
33	471038.0
34	664348.0
35	1070524.0
36	2576685.0
37	8790858.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	39.87243272688226	22.79231241689937	13.709030623902557	23.626224232315813
2	15.880249218320714	21.22045328656979	40.122432107259456	22.776865387850048
3	18.329397831354193	26.834188425805905	29.615499781956444	25.220913960883458
4	13.54035924859381	18.217841559702467	37.75264617454594	30.48915301715779
5	14.035736009987183	37.57324239859911	35.054213970323325	13.33680762109038
6	30.720938836396606	37.60861234166711	18.233228301127575	13.43722052080871
7	27.105623984614063	32.761929463881586	22.588084740552546	17.54436181095181
8	25.14058236639241	36.400200503240896	20.670797619335502	17.788419511031194
9	26.317999667346285	16.392566750290065	20.624469929437357	36.66496365292629
10-11	24.768008198045113	26.517447874817705	28.498780548827717	20.215763378309465
12-13	25.473226031633452	24.102208422757183	28.592708667357474	21.83185687825189
14-15	22.703910663922823	25.40594839231997	26.192651648662213	25.697489295094993
16-17	22.185899300777436	28.401519864403756	26.963532752960006	22.449048081858805
18-19	22.277135318223042	27.63856750839246	28.566789295047744	21.517507878336755
20-21	23.20908320922857	26.810765067675725	28.379043542149084	21.601108180946618
22-23	22.884697472851055	26.608930192102548	28.31303275290715	22.19333958213924
24-25	22.560488982652487	26.79303872609986	28.431472416359327	22.214999874888324
26-27	22.44858264743977	26.971099992807485	28.369198254352852	22.21111910539989
28-29	22.40447227413203	27.024849355452773	28.409616454945024	22.161061915470174
30-31	22.80975485116185	26.817152622542146	28.247093343464385	22.125999182831617
32-33	22.55589139878142	27.031431614489875	28.21403271357591	22.198644273152794
34-35	22.631403706343946	27.00574684809851	28.268486410465965	22.094363035091575
36-37	22.60363650014704	26.903748247086153	28.304053762765307	22.188561490001497
38-39	22.426605833617224	27.098813969195383	28.35279867693883	22.121781520248565
40-41	22.66040892235036	26.868062938991038	28.30817498436539	22.163353154293212
42-43	22.634627473959615	26.91439842581377	28.108052822669332	22.342921277557288
44-45	22.521546804315744	26.993153155756804	28.060339481404146	22.424960558523306
46-47	22.651127312174786	26.913406295295207	27.85134857031694	22.58411782221306
48-49	22.46941064626377	27.188863577936377	27.935655130011362	22.406070645788486
50-51	22.44907256708924	27.259973494941896	27.856546299392228	22.434407638576637
52-53	22.64800743511665	27.11931338331233	27.487451334643463	22.74522784692755
54-55	22.579269533276687	26.870577976108006	27.76135470333877	22.788797787276536
56-57	22.557854046880536	26.72923289127023	27.980109810377705	22.73280325147153
58-59	22.428086988367642	26.642302619202862	28.09083483871885	22.838775553710647
60-61	22.522807558815465	26.432112458925065	28.324342487488057	22.720737494771413
62-63	22.314639251363186	26.880873346813182	28.27940752045314	22.52507988137049
64-65	21.97351320758013	27.036757076425765	28.141053327792054	22.84867638820205
66-67	22.09984079837923	27.55852102125353	27.6365044958855	22.705133684481737
68-69	22.083672105956694	27.724541659583863	27.40331491712707	22.78847131733237
70-71	22.11899018234885	27.28584900901047	27.46129703761831	23.13386377102237
72-73	22.093505674545526	27.07125385798773	27.299853763486855	23.535386703979885
74-75	22.26272408299478	26.923513388707004	27.377718352425624	23.436044175872595
76-77	22.674337256987812	26.759534663066216	27.280246959199545	23.285881120746424
78-79	22.814460706761047	26.76480721138143	26.69701162875317	23.72372045310435
80-81	22.776286632751784	27.030434220440775	26.59463559850887	23.59864354829857
82-83	22.72308814607873	27.130382383393194	26.711850902883572	23.434678567644504
84-85	22.81331816675446	27.15518005810201	26.616468970663398	23.41503280448013
86-87	22.90614156953452	27.425432085846698	26.591799343286105	23.07662700133268
88-89	23.064756484673968	27.232973286914653	26.81174339618305	22.89052683222833
90-91	22.93851863609932	27.204454905612874	27.053484197090498	22.803542261197304
92-93	22.82547006774365	26.822297330927412	26.84344863816618	23.508783963162756
94-95	22.644757746628716	26.89693652584043	27.36480238362621	23.093503343904647
96-97	22.340415109343407	27.069202497765087	27.541791072217038	23.048591320674465
98-99	22.637236534189764	27.346154463847423	27.785686997190183	22.23092200477263
100-101	23.481476071436482	30.059042527902434	25.039344480463605	21.42013692019748
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2426.0
1	2462.5
2	3548.0
3	4944.0
4	6102.0
5	8283.5
6	10623.0
7	13970.0
8	17234.0
9	18857.5
10	18834.0
11	18267.5
12	19679.5
13	23625.0
14	32241.5
15	42446.0
16	49036.0
17	51427.5
18	49987.5
19	45493.5
20	39975.0
21	35667.0
22	34937.0
23	39367.5
24	48726.0
25	64099.0
26	85681.0
27	109667.0
28	134101.0
29	160369.0
30	185469.0
31	214348.5
32	250686.0
33	291245.0
34	330473.0
35	366540.0
36	408293.0
37	444604.5
38	470134.5
39	491098.0
40	517399.0
41	542783.5
42	557301.0
43	575072.5
44	594115.5
45	608429.0
46	617986.0
47	623384.5
48	625413.0
49	621376.0
50	612919.5
51	601288.5
52	581836.5
53	557713.5
54	531499.5
55	499389.0
56	461591.0
57	419508.5
58	377090.5
59	331119.0
60	277069.0
61	222880.5
62	172639.5
63	130257.0
64	97331.5
65	71903.0
66	53472.5
67	40172.5
68	30201.0
69	22007.0
70	15655.0
71	10978.0
72	7385.5
73	4681.5
74	2906.5
75	1714.0
76	1054.5
77	662.5
78	405.5
79	258.0
80	205.0
81	154.5
82	105.0
83	70.0
84	50.5
85	46.5
86	42.0
87	29.0
88	19.0
89	12.0
90	8.0
91	5.0
92	3.5
93	3.0
94	2.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.001520587855245663
4	0.0
5	0.062377595189637064
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	3.3493124564882448E-6
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	8.890763092144484E-5
44-45	0.0
46-47	0.003333384038655335
48-49	2.4078284995187482E-4
50-51	1.756278555333031E-5
52-53	8.894614385111601E-4
54-55	0.0011548306530902845
56-57	0.002208370458985243
58-59	0.005133078416363292
60-61	0.003085861528429695
62-63	0.005716190499434483
64-65	0.004273306755210254
66-67	0.00800453730496501
68-69	0.002322436662569416
70-71	0.00272122079783027
72-73	0.003304377089275952
74-75	0.0033047647709231
76-77	8.303668791110002E-4
78-79	3.2368734879040706E-4
80-81	0.0
82-83	1.086307562613313E-4
84-85	0.0017734482151946693
86-87	0.012272559560438484
88-89	0.0033315832214127514
90-91	0.0027529811299064886
92-93	1.466292979185564E-4
94-95	0.0010210543044438095
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	114.0
20-21	453.0
22-23	958.0
24-25	1205.0
26-27	2677.0
28-29	6596.0
30-31	22199.0
32-33	29298.0
34-35	39457.0
36-37	62288.0
38-39	45202.0
40-41	76403.0
42-43	84527.0
44-45	100703.0
46-47	103614.0
48-49	95734.0
50-51	89030.0
52-53	8263.0
54-55	7208.0
56-57	7186.0
58-59	7299.0
60-61	100570.0
62-63	99704.0
64-65	105578.0
66-67	111641.0
68-69	113052.0
70-71	113582.0
72-73	107607.0
74-75	110469.0
76-77	115921.0
78-79	120195.0
80-81	122498.0
82-83	116823.0
84-85	116307.0
86-87	121336.0
88-89	125810.0
90-91	129254.0
92-93	129812.0
94-95	141021.0
96-97	230180.0
98-99	1420070.0
100-101	1.0386593E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.77518820841995
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.7636578253999	73.47428482565265
2	7.724070066266021	12.787227069403853
3	1.8498206069567227	4.593577466779691
4	0.704637265788815	2.3330592917734236
5	0.34208381855829806	1.415802623210905
6	0.19769848396374218	0.9818717531170831
7	0.11083986299892815	0.6422353364512232
8	0.07773132034178515	0.5147379736784199
9	0.04518601132239729	0.3366252532439307
>10	0.17779202348126716	2.2860169863259494
>50	0.00455463141479564	0.257585078778928
>100	0.0018068854530062675	0.256555899790376
>500	6.06250080654072E-5	0.02899115150792651
>1k	6.057304633173009E-5	0.09142929028567492
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	6.6986249129764895E-6	0.0
2	0.0	6.6986249129764895E-6	0.0	1.3397249825952979E-5	0.0
3	0.0	6.6986249129764895E-6	0.0	1.3397249825952979E-5	0.0
4	0.0	6.698624912976488E-5	0.0	2.0095874738929467E-5	0.0
5	0.0	8.038349895571787E-5	0.0	2.0095874738929467E-5	0.0
6	4.6890374390835425E-5	8.038349895571787E-5	0.0	2.0095874738929467E-5	0.0
7	4.6890374390835425E-5	8.038349895571787E-5	0.0	4.6890374390835425E-5	0.0
8	4.6890374390835425E-5	8.038349895571787E-5	0.0	4.6890374390835425E-5	0.0
9	4.6890374390835425E-5	2.0765737230227116E-4	0.0	7.368487404274138E-5	0.0
10-11	4.6890374390835425E-5	2.1435599721524766E-4	0.0	9.043143632518261E-5	0.0
12-13	4.6890374390835425E-5	2.1435599721524766E-4	0.0	1.0717799860762383E-4	1.0047937369464733E-5
14-15	4.6890374390835425E-5	3.5837643284424215E-4	0.0	1.1052731106411207E-4	3.01438121083942E-5
16-17	6.02876242167884E-5	3.751229951266834E-4	0.0	1.1387662352060031E-4	3.349312456488244E-5
18-19	6.02876242167884E-5	4.0191749477858936E-4	0.0	1.1722593597708855E-4	4.6890374390835425E-5
20-21	6.02876242167884E-5	4.354106193434718E-4	0.0	1.440204356289945E-4	5.023968684732367E-5
22-23	6.02876242167884E-5	5.157941182991896E-4	0.0	1.7081493528090048E-4	6.02876242167884E-5
24-25	6.02876242167884E-5	5.660338051465133E-4	0.0	1.9426012247631818E-4	7.368487404274138E-5
26-27	6.02876242167884E-5	5.961776172549075E-4	0.0	2.411504968671536E-4	7.368487404274138E-5
28-29	6.02876242167884E-5	6.028762421678841E-4	0.0	5.425886179510957E-4	7.368487404274138E-5
30-31	6.363693667327665E-5	6.732118037541372E-4	0.0	0.0016612589784181693	7.368487404274138E-5
32-33	6.698624912976488E-5	6.832597411236019E-4	0.0	0.004561763565736989	8.038349895571787E-5
34-35	6.698624912976488E-5	7.770404899052727E-4	0.0	0.008446966015263353	9.378074878167085E-5
36-37	6.698624912976488E-5	8.842184885128966E-4	0.0	0.014241276564988015	9.378074878167085E-5
38-39	6.698624912976488E-5	9.210609255342673E-4	0.0	0.0342199253679404	1.038286861511356E-4
40-41	7.033556158625312E-5	0.0010081430494029617	0.0	0.2794699806818356	1.2392456089006505E-4
42-43	7.368487404274138E-5	0.0010483347988808206	0.0	0.2990500613024659	1.272738733465533E-4
44-45	7.703418649922963E-5	0.0011655607348579093	0.0	0.33398673953609476	1.272738733465533E-4
46-47	8.038349895571787E-5	0.0012526428587266035	0.0	0.37314355146489886	1.3397249825952977E-4
48-49	8.038349895571787E-5	0.0012559921711830916	0.0	0.41027068004507106	1.4067112317250627E-4
50-51	8.038349895571787E-5	0.0014167591690945275	0.0	0.4466341653851639	1.4067112317250627E-4
52-53	8.038349895571787E-5	0.0014268071064639921	0.0	0.6098696065770315	1.674656228244122E-4
54-55	8.038349895571787E-5	0.001440204356289945	0.0	1.180994366657407	2.0430805984578292E-4
56-57	8.038349895571787E-5	0.0014669988559418512	0.0	1.8039664835642204	2.177053096717359E-4
58-59	8.038349895571787E-5	0.0014736974808548277	0.0	2.446612461840446	2.2105462212822414E-4
60-61	1.0047937369464734E-4	0.001533985105071616	0.0	2.9281263671474784	2.3780118441066536E-4
62-63	1.0717799860762383E-4	0.001560779604723522	0.0	2.935963758295661	2.411504968671536E-4
64-65	1.0717799860762383E-4	0.0017550397271998402	0.0	2.941349452725694	2.411504968671536E-4
66-67	1.1722593597708855E-4	0.0017650876645693048	0.0	2.9469227086532905	2.4449980932364187E-4
68-69	1.272738733465533E-4	0.0018287246012425817	0.0	2.952057204649087	2.5119843423661835E-4
70-71	1.272738733465533E-4	0.0018555191008944876	0.0	2.9566223175272803	2.7464362143203607E-4
72-73	1.3397249825952977E-4	0.001875614975633417	0.0	2.960152492856419	3.0143812108394204E-4
74-75	1.4067112317250627E-4	0.001942601224763182	0.0	2.9632707027534098	3.0143812108394204E-4
76-77	1.4067112317250627E-4	0.0020698750981097353	0.0	2.9662817346517922	3.0143812108394204E-4
78-79	1.4067112317250627E-4	0.002110066847587594	0.0	2.969249225488241	3.0143812108394204E-4
80-81	1.4067112317250627E-4	0.002110066847587594	0.0	2.9704951697220547	3.1148605845340675E-4
82-83	1.4067112317250627E-4	0.002113416160044082	0.0	2.9711047445891356	3.315819331923362E-4
84-85	1.4067112317250627E-4	0.0021301627223265234	0.0	2.9715167100212834	3.4162987056180096E-4
86-87	1.4067112317250627E-4	0.002177053096717359	0.0	2.9717143194562166	3.4162987056180096E-4
88-89	1.4067112317250627E-4	0.0022775324704120063	0.0	2.9717779563928897	3.449791830182892E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	18815	0.0	53.71474	1
GTATCAA	33815	0.0	38.12183	1
TATCTCG	14645	0.0	34.5851	94-95
TCAACGC	41915	0.0	31.14521	4
ATCAACG	42710	0.0	30.659168	3
TATCAAC	43500	0.0	30.244843	2
CAACGCA	43540	0.0	30.01331	5
AACGCAG	45235	0.0	28.949509	6
CCGTCTT	7630	0.0	27.834005	92-93
TATGCCG	7550	0.0	27.232298	88-89
TGCCGTC	7975	0.0	26.241125	90-91
TCTCGTA	7245	0.0	25.547218	82-83
GCCGTCT	7940	0.0	25.075323	90-91
ACGCAGA	52795	0.0	24.762173	7
ATGCCGT	8005	0.0	24.598694	88-89
CGCAGAG	54150	0.0	24.126205	8
CTCGTAT	8525	0.0	23.681145	84-85
CGTATGC	8840	0.0	23.258951	86-87
TCGTATG	8730	0.0	22.363367	84-85
CTATCTC	23300	0.0	21.847795	94-95
>>END_MODULE