FastQCFastQC Report
Fri 27 May 2016
SRR2140351_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140351_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12802345
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA155500.052.5686341
GTATCAA291250.039.096671
TCAACGC368100.030.9102064
ATCAACG372300.030.5379473
CAACGCA379750.029.9734275
TATCAAC388500.029.6048342
AACGCAG392950.028.9716636
ACGCAGA448500.025.393137
CGCAGAG456500.024.9674198
GTGGTAT73700.022.7210941
TGGTATC71550.021.9257012
GCAGAGT538650.021.0860319
GTACATG368600.020.108691
GAGTACT319700.020.05713512-13
AGAGTAC480800.019.92255210-11
TACATGG368950.019.8746262
ACATGGG372800.019.1610953
CAGAGTA522600.018.75041210-11
GTACTTT354100.018.05287614-15
AGTACTT351500.017.9356112-13
Produced by FastQC (version 0.11.5)