##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2140351_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 12802345 Sequences flagged as poor quality 0 Sequence length 19-101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.928124339720576 31.0 25.0 33.0 18.0 33.0 2 30.93020059996821 33.0 30.0 33.0 27.0 33.0 3 31.40532480572895 33.0 31.0 33.0 28.0 34.0 4 31.93874497211253 33.0 33.0 33.0 29.0 34.0 5 31.946632042801532 33.0 32.0 33.0 30.0 34.0 6 35.38635937400531 38.0 35.0 38.0 29.0 38.0 7 35.92339419067366 38.0 36.0 38.0 31.0 38.0 8 36.091778342170905 38.0 37.0 38.0 33.0 38.0 9 36.62929635156684 38.0 38.0 38.0 34.0 38.0 10-11 36.745664290409294 38.0 38.0 38.0 34.0 38.0 12-13 36.827318549843795 38.0 38.0 38.0 35.0 38.0 14-15 36.82581855121073 38.0 38.0 38.0 35.0 38.0 16-17 36.84869767999534 38.0 38.0 38.0 35.0 38.0 18-19 36.8831420337446 38.0 38.0 38.0 35.0 38.0 20-21 36.90880189310329 38.0 38.0 38.0 35.0 38.0 22-23 36.93425983489671 38.0 38.0 38.0 35.0 38.0 24-25 36.940831077669024 38.0 38.0 38.0 35.0 38.0 26-27 36.91311706962388 38.0 38.0 38.0 35.0 38.0 28-29 36.901954883709095 38.0 38.0 38.0 35.0 38.0 30-31 36.89406317782448 38.0 38.0 38.0 35.0 38.0 32-33 36.87632827716125 38.0 38.0 38.0 35.0 38.0 34-35 36.86324598272892 38.0 38.0 38.0 35.0 38.0 36-37 36.83834156555777 38.0 38.0 38.0 35.0 38.0 38-39 36.821804703914665 38.0 38.0 38.0 35.0 38.0 40-41 36.79942848159321 38.0 38.0 38.0 35.0 38.0 42-43 36.76622330920843 38.0 38.0 38.0 35.0 38.0 44-45 36.73038697707722 38.0 38.0 38.0 35.0 38.0 46-47 36.67663076663278 38.0 38.0 38.0 34.0 38.0 48-49 36.642945153012974 38.0 38.0 38.0 34.0 38.0 50-51 36.61232642650126 38.0 38.0 38.0 34.0 38.0 52-53 36.50803433158262 38.0 38.0 38.0 34.0 38.0 54-55 36.541270967534544 38.0 38.0 38.0 34.0 38.0 56-57 36.5320700483722 38.0 38.0 38.0 34.0 38.0 58-59 36.52049001832256 38.0 38.0 38.0 34.0 38.0 60-61 36.48801741307771 38.0 38.0 38.0 34.0 38.0 62-63 36.497291690340205 38.0 38.0 38.0 34.0 38.0 64-65 36.49783466946469 38.0 38.0 38.0 34.0 38.0 66-67 36.49221699923376 38.0 38.0 38.0 34.0 38.0 68-69 36.48000442931054 38.0 38.0 38.0 34.0 38.0 70-71 36.47970279479427 38.0 38.0 38.0 34.0 38.0 72-73 36.46297215939794 38.0 38.0 38.0 34.0 38.0 74-75 36.451377698502235 38.0 38.0 38.0 34.0 38.0 76-77 36.434858466331974 38.0 38.0 38.0 34.0 38.0 78-79 36.4201939471961 38.0 38.0 38.0 34.0 38.0 80-81 36.40502670616898 38.0 38.0 38.0 34.0 38.0 82-83 36.38791487438657 38.0 38.0 38.0 34.0 38.0 84-85 36.37977010657248 38.0 38.0 38.0 34.0 38.0 86-87 36.35741622420743 38.0 38.0 38.0 34.0 38.0 88-89 36.33176480839319 38.0 37.5 38.0 33.5 38.0 90-91 36.32258256170147 38.0 37.0 38.0 33.0 38.0 92-93 36.32556857976387 38.0 37.0 38.0 33.5 38.0 94-95 36.30934810600583 38.0 37.0 38.0 33.0 38.0 96-97 36.298563107717726 38.0 37.0 38.0 33.0 38.0 98-99 36.31336470963086 38.0 37.0 38.0 33.0 38.0 100-101 35.75818782792733 38.0 36.0 38.0 31.5 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 2.0 18 6.0 19 15.0 20 77.0 21 552.0 22 2286.0 23 7164.0 24 16202.0 25 31222.0 26 51608.0 27 78727.0 28 112640.0 29 151384.0 30 196697.0 31 252716.0 32 322595.0 33 424532.0 34 592458.0 35 933969.0 36 2053125.0 37 7574368.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.01508317421535 20.33279059422317 15.891877620857741 23.76024861070374 2 14.702697044955437 21.935989070752274 40.62631494464491 22.734998939647387 3 18.028787438640336 25.849790750497903 31.25167500756316 24.869746803298597 4 12.760506982158892 18.138350289603217 38.19551325913442 30.905629469103467 5 13.615435328102718 37.27866055416586 35.320755837741785 13.785148279989636 6 30.200185981552597 38.05719967709041 18.371368682846775 13.371245658510219 7 26.558837462980417 32.720482067933645 22.9141926732954 17.806487795790538 8 24.590604299446703 36.777527866965 20.87351184490029 17.75835598868801 9 25.683130707694566 16.69903443470708 21.02175812321883 36.59607673437952 10-11 24.3846810877226 26.723881445157115 28.7242102911615 20.16722717595878 12-13 25.16123708132031 24.295165178156637 28.874311175918688 21.669286564604363 14-15 22.242702411159833 25.662732101033054 26.557365076476223 25.53720041133089 16-17 21.791547634107175 28.69627512366973 27.262947813972666 22.249229428250427 18-19 21.842701473831553 27.78538228738563 29.118149838955286 21.25376639982753 20-21 22.61567220562538 26.98707180532498 29.016291852246056 21.38096413680358 22-23 22.35232892800019 26.83675488855119 28.909162428170543 21.90175375527808 24-25 22.096335638322245 26.988947935781244 29.013751536734144 21.90096488916237 26-27 22.01741379691576 27.162428371309627 28.946839383671165 21.873318448103447 28-29 21.98700598628073 27.17857810641743 28.99631374527349 21.838102162028353 30-31 22.27574961287598 27.021628110678364 28.892238749941345 21.81038352650431 32-33 22.10077576116711 27.18348206993861 28.832630974189033 21.883111194705247 34-35 22.216250500014027 27.201673337770927 28.832200687532772 21.74987547468227 36-37 22.16549121140178 27.12641252249289 28.851057547751136 21.8570387183542 38-39 22.023735792376122 27.281858040024048 28.901702494781013 21.792703672818817 40-41 22.263842421944233 27.104531048205498 28.79812293146216 21.83350359838811 42-43 22.129550466559277 27.191458173829126 28.630234642885803 22.04875671672579 44-45 22.180221394847557 27.273232040293404 28.518828800362545 22.02771776449649 46-47 22.29323236594372 27.256093452625528 28.26921563707322 22.18145854435754 48-49 22.16532488188858 27.41070203228701 28.29528576996529 22.128687315859125 50-51 22.179609421786456 27.51680062683392 28.166581965645726 22.1370079857339 52-53 22.35990173301756 27.423406793046407 27.996722733522617 22.219968740413417 54-55 22.28740014409449 27.36146414095602 28.08940144541967 22.261734269529814 56-57 22.25592288459554 27.452677045387418 28.10786916404921 22.18353090596783 58-59 22.25557915475556 27.466873530038455 28.10109406954805 22.17645324565794 60-61 22.327801473027982 27.43979994340531 28.15425109739566 22.07814748617105 62-63 22.288235585876297 27.52568356254962 28.103424739026185 22.082656112547898 64-65 22.243667740206995 27.45821043538479 28.059346986747734 22.238774837660483 66-67 22.29081142638705 27.554044305342597 27.914600726279527 22.240543541990824 68-69 22.232829409120768 27.50664263734744 27.991256958130208 22.269270995401584 70-71 22.255869021994897 27.28094106894507 28.066616483823214 22.396573425236816 72-73 22.29928948354156 27.363836975704714 27.939384734823015 22.39748880593071 74-75 22.15372035384847 27.482333811333458 27.96332990541816 22.400615929399905 76-77 22.35423422724345 27.37580229529023 27.885117312243366 22.384846165222953 78-79 22.323573086910248 27.43174546043402 27.796981684329868 22.44769976832586 80-81 22.378044132615763 27.485984934556274 27.733888314764275 22.402082618063684 82-83 22.344413872602097 27.470025705112967 27.826957345556803 22.358603076728134 84-85 22.43281880414175 27.484602143650843 27.774702788234706 22.3078762639727 86-87 22.321313548276997 27.520377867158647 27.807561021266746 22.350747563297606 88-89 22.35776742664159 27.53475779364422 27.79182739453674 22.315647385177453 90-91 22.423533604484746 27.534949870929665 27.80625213310178 22.23526439148381 92-93 22.513038214090102 27.48280824595199 27.7641023386571 22.240051201300815 94-95 22.364693204824317 27.57178777722149 27.77952018142088 22.283998836533314 96-97 22.405329373590057 27.588791526636353 27.79055076892846 22.21532833084514 98-99 22.583750287773963 28.166392592654464 27.85716468512965 21.39269243444193 100-101 23.216898344469673 30.641089869315714 25.23770410423828 20.904307681976338 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3546.0 1 3155.5 2 3689.5 3 4875.0 4 5807.5 5 7843.0 6 10244.0 7 14818.0 8 19063.0 9 20163.0 10 19199.5 11 17829.0 12 18970.5 13 22786.0 14 31447.0 15 42381.0 16 49724.5 17 52020.5 18 50325.5 19 46259.0 20 41685.5 21 38238.5 22 38173.5 23 42947.0 24 52026.5 25 66901.5 26 86849.0 27 108259.0 28 132016.0 29 158187.5 30 183724.5 31 212879.5 32 246159.5 33 280049.0 34 312787.0 35 341788.0 36 375040.5 37 405844.5 38 428020.5 39 445336.0 40 463079.5 41 482373.5 42 494357.5 43 503988.5 44 515213.5 45 523402.0 46 524268.5 47 522683.0 48 521241.0 49 512773.5 50 501404.0 51 488452.5 52 468924.5 53 445775.5 54 420240.5 55 392551.0 56 362981.5 57 330299.5 58 296151.0 59 257572.5 60 214204.0 61 173313.0 62 136752.0 63 104669.0 64 78189.5 65 58066.5 66 43115.5 67 32696.5 68 24870.0 69 18195.0 70 13119.5 71 9445.0 72 6621.5 73 4473.5 74 2941.5 75 1820.5 76 1107.0 77 640.5 78 413.5 79 293.5 80 200.5 81 131.0 82 96.0 83 68.5 84 48.5 85 42.0 86 36.5 87 31.0 88 23.5 89 15.0 90 9.0 91 6.0 92 4.5 93 3.0 94 1.0 95 0.5 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0012810153139913038 4 7.811068987751853E-6 5 0.06092633810446446 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 1.171660348162778E-5 14-15 0.0 16-17 3.905534493875927E-6 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 1.475719174789005E-4 44-45 0.0 46-47 0.003275833898660402 48-49 3.049732599445681E-4 50-51 1.227353236066135E-5 52-53 7.939689297331737E-4 54-55 0.0010711231565825729 56-57 0.0022667471438154157 58-59 0.0050159787580202325 60-61 0.002790927809221436 62-63 0.005688098076194594 64-65 0.0042534050894421195 66-67 0.007925339270601035 68-69 0.002342371574292377 70-71 0.0025730780282646744 72-73 0.003368073756956026 74-75 0.0031578104510806356 76-77 7.741912748999269E-4 78-79 2.870076523863767E-4 80-81 0.0 82-83 8.660284128424903E-5 84-85 0.0015666962822802608 86-87 0.010194784772391356 88-89 0.0027001693211713596 90-91 0.0022909695354790573 92-93 1.379753637851305E-4 94-95 7.636632686853152E-4 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 18-19 119.0 20-21 549.0 22-23 1142.0 24-25 1274.0 26-27 2541.0 28-29 5972.0 30-31 17513.0 32-33 23000.0 34-35 31166.0 36-37 49596.0 38-39 57559.0 40-41 59510.0 42-43 69350.0 44-45 82919.0 46-47 84468.0 48-49 76631.0 50-51 69622.0 52-53 62800.0 54-55 67397.0 56-57 68863.0 58-59 73945.0 60-61 73234.0 62-63 72327.0 64-65 78282.0 66-67 82238.0 68-69 84300.0 70-71 84927.0 72-73 79930.0 74-75 82242.0 76-77 87051.0 78-79 89566.0 80-81 90754.0 82-83 87034.0 84-85 87582.0 86-87 90372.0 88-89 95343.0 90-91 98057.0 92-93 97330.0 94-95 107477.0 96-97 182770.0 98-99 1115213.0 100-101 9030380.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 84.04905576928144 #Duplication Level Percentage of deduplicated Percentage of total 1 89.64153988161965 75.34286784754516 2 7.077051886547654 11.896390573890844 3 1.7375261889278415 4.381123066613496 4 0.6564513601093385 2.2069646790260173 5 0.3188105296887425 1.3397861994821636 6 0.1721747450606789 0.8682674849800087 7 0.11448953263494116 0.6735915979403195 8 0.06818002518454383 0.458437339126939 9 0.047710594245517636 0.3609027356874363 >10 0.16166948507412335 2.0728103731992262 >50 0.00339536846319917 0.19353204151443304 >100 9.010954798038228E-4 0.12129302440593934 >500 4.965892111059267E-5 0.025751936827941472 >1k 4.964804284495909E-5 0.05828109976013532 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 2.343320696325556E-5 0.0 0.0 0.0 3 0.0 2.343320696325556E-5 0.0 0.0 0.0 4 0.0 8.592175886527038E-5 0.0 7.811068987751853E-6 7.811068987751853E-6 5 0.0 8.592175886527038E-5 0.0 7.811068987751853E-6 7.811068987751853E-6 6 4.686641392651112E-5 8.592175886527038E-5 0.0 7.811068987751853E-6 2.343320696325556E-5 7 4.686641392651112E-5 8.592175886527038E-5 0.0 7.811068987751853E-6 2.343320696325556E-5 8 4.686641392651112E-5 1.0935496582852594E-4 0.0 1.5622137975503707E-5 2.343320696325556E-5 9 4.686641392651112E-5 1.8746565570604447E-4 0.0 5.467748291426297E-5 3.905534493875927E-5 10-11 5.0771948420387045E-5 1.8746565570604447E-4 0.0 6.248855190201483E-5 3.905534493875927E-5 12-13 5.467748291426297E-5 1.8746565570604447E-4 0.0 7.029962088976668E-5 4.296087943263519E-5 14-15 5.467748291426297E-5 2.0308779368154818E-4 0.0 7.029962088976668E-5 7.029962088976668E-5 16-17 8.592175886527038E-5 2.1089886266930003E-4 0.0 7.029962088976668E-5 8.201622437139445E-5 18-19 8.592175886527038E-5 2.2652100064480374E-4 0.0 8.201622437139445E-5 9.763836234689816E-5 20-21 8.592175886527038E-5 2.499542076080593E-4 0.0 9.763836234689816E-5 1.0544943133465002E-4 22-23 8.592175886527038E-5 2.8510401805294266E-4 0.0 1.0544943133465002E-4 1.2888263829790558E-4 24-25 8.982729335914631E-5 3.280648974855778E-4 0.0 1.327881727917815E-4 1.6403244874278893E-4 26-27 9.373282785302223E-5 3.5149810444883337E-4 0.0 1.6793798323666483E-4 1.7184351773054076E-4 28-29 1.0154389684077409E-4 3.5149810444883337E-4 0.0 3.866479148937167E-4 1.7184351773054076E-4 30-31 1.0935496582852594E-4 3.983645183753445E-4 0.0 0.001445047762734093 1.7184351773054076E-4 32-33 1.0935496582852594E-4 4.0617558736309636E-4 0.0 0.0036594858207617434 1.7965458671829261E-4 34-35 1.2497710380402966E-4 4.7647520825286306E-4 0.0 0.006854213036752251 1.7965458671829261E-4 36-37 1.2497710380402966E-4 5.545858981303816E-4 0.0 0.011595531912317627 1.7965458671829261E-4 38-39 1.2497710380402966E-4 5.741135705997612E-4 0.0 0.02005882516054676 1.8746565570604447E-4 40-41 1.2497710380402966E-4 6.795630019344113E-4 0.0 0.042890579811745426 2.069933281754241E-4 42-43 1.2497710380402966E-4 7.029962088976667E-4 0.0 0.05164678814701525 2.1089886266930003E-4 44-45 1.2888263829790558E-4 8.201622437139446E-4 0.0 0.06303532673115746 2.187099316570519E-4 46-47 1.327881727917815E-4 9.217061405547187E-4 0.0 0.07592359056094802 2.343320696325556E-4 48-49 1.327881727917815E-4 9.256116750485946E-4 0.0 0.0881752522682368 2.343320696325556E-4 50-51 1.4059924177953337E-4 0.0010505887788526242 0.0 0.10041910290653783 2.343320696325556E-4 52-53 1.4059924177953337E-4 0.0010544943133465002 0.0 0.11875168182079142 2.460486731141834E-4 54-55 1.4059924177953337E-4 0.0010818330548036316 0.0 0.16106033699294933 2.5776527659581117E-4 56-57 1.4059924177953337E-4 0.0010935496582852595 0.0 0.2072393768485383 2.5776527659581117E-4 58-59 1.4059924177953337E-4 0.001124793934236267 0.0 0.2603155906203122 2.616708110896871E-4 60-61 1.4841031076728522E-4 0.0011560382101872743 0.0 0.27576197954359144 2.733874145713149E-4 62-63 1.7184351773054076E-4 0.0011677548136689022 0.0 0.288333895079378 2.733874145713149E-4 64-65 1.7184351773054076E-4 0.0013513149348810707 0.0 0.2952388800645507 2.733874145713149E-4 66-67 1.9527672469379632E-4 0.0013591260038688226 0.0 0.3003980911309608 2.772929490651908E-4 68-69 1.9527672469379632E-4 0.001382559210832078 0.0 0.30547138043850564 2.8510401805294266E-4 70-71 1.9918225918767225E-4 0.0013903702798198298 0.0 0.3100955332792547 2.9682062153457044E-4 72-73 2.2652100064480374E-4 0.0013903702798198298 0.0 0.31395420135920415 3.5930917343658523E-4 74-75 2.3042653513867967E-4 0.001437236693746341 0.0 0.3169731795229702 3.5930917343658523E-4 76-77 2.343320696325556E-4 0.0015075363146361076 0.0 0.3196953370652017 3.6321470793046116E-4 78-79 2.343320696325556E-4 0.0015309695215993632 0.0 0.3221441071928619 3.671202424243371E-4 80-81 2.343320696325556E-4 0.0015348750560932392 0.0 0.32331576754102476 3.827423803998408E-4 82-83 2.343320696325556E-4 0.0015465916595748669 0.0 0.32378833721478373 3.983645183753445E-4 84-85 2.5776527659581117E-4 0.0015583082630564946 0.0 0.3242413792160733 3.983645183753445E-4 86-87 2.616708110896871E-4 0.001593458073501378 0.0 0.3244210338027916 3.983645183753445E-4 88-89 2.65576345583563E-4 0.0016559466254033929 0.0 0.324471805751212 3.983645183753445E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 15550 0.0 52.568634 1 GTATCAA 29125 0.0 39.09667 1 TCAACGC 36810 0.0 30.910206 4 ATCAACG 37230 0.0 30.537947 3 CAACGCA 37975 0.0 29.973427 5 TATCAAC 38850 0.0 29.604834 2 AACGCAG 39295 0.0 28.971663 6 ACGCAGA 44850 0.0 25.39313 7 CGCAGAG 45650 0.0 24.967419 8 GTGGTAT 7370 0.0 22.721094 1 TGGTATC 7155 0.0 21.925701 2 GCAGAGT 53865 0.0 21.086031 9 GTACATG 36860 0.0 20.10869 1 GAGTACT 31970 0.0 20.057135 12-13 AGAGTAC 48080 0.0 19.922552 10-11 TACATGG 36895 0.0 19.874626 2 ACATGGG 37280 0.0 19.161095 3 CAGAGTA 52260 0.0 18.750412 10-11 GTACTTT 35410 0.0 18.052876 14-15 AGTACTT 35150 0.0 17.93561 12-13 >>END_MODULE