FastQCFastQC Report
Fri 27 May 2016
SRR2140350_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140350_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11170781
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA143300.053.1446531
GTATCAA271650.039.6592521
TCAACGC341850.031.8872494
ATCAACG347800.031.329533
CAACGCA353250.030.895495
TATCAAC359500.030.4687232
AACGCAG365200.029.8551246
ACGCAGA422300.025.8079627
CGCAGAG431050.025.2738958
GCAGAGT498550.021.8167829
GTGGTAT68650.021.176011
TGGTATC65250.021.0678632
GTACATG367300.020.2079581
GAGTACT282750.020.17305812-13
TACATGG366150.020.0675142
AGAGTAC449950.019.71515310-11
ACATGGG369900.019.4366343
CAGAGTA483750.019.1678710-11
GTACTTT309050.018.32864214-15
AGTACTT306000.017.95177812-13
Produced by FastQC (version 0.11.5)