FastQCFastQC Report
Fri 27 May 2016
SRR2140349_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140349_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12270668
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA155100.051.3878331
GTATCAA281200.038.510311
TCAACGC351250.030.918124
ATCAACG354300.030.7271523
CAACGCA363200.029.9130345
TATCAAC370600.029.542872
AACGCAG372600.029.1910176
ACGCAGA429550.025.2693717
CGCAGAG433150.025.0695618
GTGGTAT74000.023.2037621
TGGTATC71500.022.7152942
GCAGAGT511250.021.1619999
GTACATG343600.019.950631
TACATGG344000.019.9017032
AGAGTAC457950.019.86780210-11
GAGTACT312300.019.72934712-13
ACATGGG345100.018.9919033
CAGAGTA497800.018.8815510-11
AGTACTT338400.017.9527912-13
GTACTTT345100.017.77129714-15
Produced by FastQC (version 0.11.5)