FastQCFastQC Report
Fri 27 May 2016
SRR2140348_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140348_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11777685
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA132150.051.215791
GTATCAA236300.036.7400741
TCAACGC286200.030.34944
ATCAACG290250.029.8957773
CAACGCA296100.029.2898035
TATCAAC307650.028.4928932
AACGCAG308600.028.1444386
ACGCAGA358650.024.1674797
CGCAGAG364850.023.7689278
GTGGTAT68700.021.3412061
TGGTATC62800.021.230222
GTACATG318750.020.2746221
TACATGG317350.020.1407452
GCAGAGT437400.019.8568219
ACATGGG318450.019.4313433
GAGTACT255900.019.16721212-13
AGAGTAC388600.018.830410-11
CAGAGTA422500.018.18915410-11
TATCTCG196450.017.7520494-95
CATGGGG235500.017.1344284
Produced by FastQC (version 0.11.5)