FastQCFastQC Report
Fri 27 May 2016
SRR2140347_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140347_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9659554
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA117950.051.349671
GTATCAA224000.041.0435261
TCAACGC273800.033.318884
ATCAACG274100.033.0397643
CAACGCA281250.032.4047475
TATCAAC283450.032.32562
AACGCAG291500.031.3653036
ACGCAGA336050.027.154447
CGCAGAG342600.026.6741168
GTGGTAT58050.024.9182971
TGGTATC57400.023.4225252
GCAGAGT400150.022.760259
GAGTACT228400.020.5006312-13
GTACATG292450.020.3763941
TACATGG292200.019.8016052
AGAGTAC358400.019.72676710-11
CAGAGTA387750.019.44001210-11
ACATGGG295050.018.9185563
GTACTTT252950.018.36209114-15
AGTACTT249250.017.90515312-13
Produced by FastQC (version 0.11.5)