##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140347_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9659554
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.863226811507033	32.0	30.0	33.0	18.0	33.0
2	31.38719272132026	33.0	31.0	33.0	27.0	34.0
3	30.515462722191934	33.0	30.0	33.0	25.0	33.0
4	31.52525168346282	33.0	31.0	33.0	29.0	34.0
5	31.37876158671508	33.0	32.0	33.0	28.0	33.0
6	34.49851773694728	37.0	34.0	38.0	28.0	38.0
7	36.007906990322745	38.0	36.0	38.0	31.0	38.0
8	36.009855113393435	38.0	36.0	38.0	31.0	38.0
9	36.53847900223965	38.0	37.0	38.0	34.0	38.0
10-11	36.72492177175054	38.0	38.0	38.0	34.0	38.0
12-13	36.80608473227646	38.0	38.0	38.0	35.0	38.0
14-15	36.85975910481996	38.0	38.0	38.0	35.0	38.0
16-17	36.87752063915167	38.0	38.0	38.0	35.0	38.0
18-19	36.90451629547286	38.0	38.0	38.0	35.0	38.0
20-21	36.93109474792795	38.0	38.0	38.0	35.0	38.0
22-23	36.945907707968175	38.0	38.0	38.0	35.0	38.0
24-25	36.95449268531635	38.0	38.0	38.0	35.0	38.0
26-27	36.924420997870016	38.0	38.0	38.0	35.0	38.0
28-29	36.91542194033089	38.0	38.0	38.0	35.0	38.0
30-31	36.90528500428792	38.0	38.0	38.0	35.0	38.0
32-33	36.87681686973584	38.0	38.0	38.0	35.0	38.0
34-35	36.85707201699198	38.0	38.0	38.0	35.0	38.0
36-37	36.83051483370235	38.0	38.0	38.0	35.0	38.0
38-39	36.79379167684894	38.0	38.0	38.0	35.0	38.0
40-41	36.80511958235407	38.0	38.0	38.0	35.0	38.0
42-43	36.76809947513411	38.0	38.0	38.0	35.0	38.0
44-45	36.73080145784649	38.0	38.0	38.0	35.0	38.0
46-47	36.67790416672065	38.0	38.0	38.0	34.0	38.0
48-49	36.63963965070843	38.0	38.0	38.0	34.0	38.0
50-51	36.6102795461196	38.0	38.0	38.0	34.0	38.0
52-53	36.50984301360455	38.0	38.0	38.0	34.0	38.0
54-55	36.545492567685244	38.0	38.0	38.0	34.0	38.0
56-57	36.54145563551997	38.0	38.0	38.0	34.0	38.0
58-59	36.52814353392181	38.0	38.0	38.0	34.0	38.0
60-61	36.493996817319726	38.0	38.0	38.0	34.0	38.0
62-63	36.50584526461964	38.0	38.0	38.0	34.0	38.0
64-65	36.51163620505724	38.0	38.0	38.0	34.0	38.0
66-67	36.501208572454516	38.0	38.0	38.0	34.0	38.0
68-69	36.48719936989654	38.0	38.0	38.0	34.0	38.0
70-71	36.48136747271566	38.0	38.0	38.0	34.0	38.0
72-73	36.46165663629023	38.0	38.0	38.0	34.0	38.0
74-75	36.45194754401547	38.0	38.0	38.0	34.0	38.0
76-77	36.43194177069394	38.0	38.0	38.0	34.0	38.0
78-79	36.41504017477208	38.0	38.0	38.0	34.0	38.0
80-81	36.39790099561247	38.0	38.0	38.0	34.0	38.0
82-83	36.369582297971775	38.0	38.0	38.0	34.0	38.0
84-85	36.37164690319882	38.0	38.0	38.0	34.0	38.0
86-87	36.35534084812228	38.0	38.0	38.0	34.0	38.0
88-89	36.33146724345676	38.0	38.0	38.0	33.5	38.0
90-91	36.331235476313026	38.0	38.0	38.0	33.0	38.0
92-93	36.34366707863959	38.0	38.0	38.0	34.0	38.0
94-95	36.31946935947574	38.0	37.5	38.0	33.5	38.0
96-97	36.266588171843054	38.0	37.0	38.0	33.0	38.0
98-99	36.27425672732411	38.0	37.0	38.0	33.0	38.0
100-101	35.75735671921361	38.0	36.0	38.0	31.5	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	6.0
19	30.0
20	83.0
21	402.0
22	1786.0
23	5431.0
24	12639.0
25	23562.0
26	39166.0
27	59820.0
28	85179.0
29	115100.0
30	149287.0
31	190376.0
32	245089.0
33	321726.0
34	448517.0
35	708445.0
36	1552199.0
37	5700711.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.98418560525672	20.31098951359452	14.242117182635969	25.462707698512787
2	14.328363400629057	20.48449648917538	42.4663706005474	22.720769509648168
3	18.084846246175488	25.411966496476694	29.930931542138943	26.57225571520887
4	12.476083264299781	18.140640861886585	38.826523460606985	30.556752413206656
5	13.80980055173814	36.84895987104701	35.25944434603077	14.081795231184083
6	29.972626065344215	37.77248928884294	18.499301313497497	13.755583332315343
7	26.639625390571865	32.69480143700216	22.475354452182785	18.19021872024319
8	24.42164514013794	36.690513868445684	20.891295809309625	17.99654518210675
9	25.554254368265866	16.956072713088	20.809252683923088	36.68042023472305
10-11	24.33239153691775	26.6596159615651	28.579352628496096	20.428639873021055
12-13	25.276520286367273	24.020913596058037	28.660889967636706	22.041676149937985
14-15	22.36712481756404	25.408678288873375	26.2063289878601	26.01786790570248
16-17	21.991367303293714	28.71587031865032	26.85978565884098	22.432976719214988
18-19	22.06187719678925	27.64090922643203	28.69962292247094	21.597590654307776
20-21	22.753735896889598	26.778729597322563	28.62692007386799	21.84061443191985
22-23	22.446180409423658	26.83259623325762	28.556449628250807	22.164773729067917
24-25	22.326409440859223	26.98682104336077	28.63465267858257	22.052116837197435
26-27	22.251367860818625	27.129683985499646	28.61017967835738	22.00876847532435
28-29	22.176395960445923	26.97365255797494	28.752953067199556	22.09699841437958
30-31	22.314675730917692	26.75503005507722	28.79865300686027	22.131641207144813
32-33	22.03011562875813	27.11437685919964	28.656659259057456	22.198848252984774
34-35	22.317552832705175	26.979598516879648	28.68552589289437	22.0173227575208
36-37	22.15332029824439	27.015713635667876	28.823023642920866	22.007942423166867
38-39	22.04238760620067	27.046845298953343	28.82376068027061	22.08700641457537
40-41	22.292886169184616	26.818877763806903	28.80410113476956	22.084134932238918
42-43	22.39157326385704	26.83491206906728	28.634518374530295	22.13899629254539
44-45	22.15843111029332	27.06985461804091	28.52687793848701	22.244836333178753
46-47	22.42494886621355	26.858916460353278	28.202380536618797	22.51375413681437
48-49	22.256739395698613	27.070132718388674	28.23281353308284	22.440314352829873
50-51	22.07987412474931	27.19557896281965	28.33698325912602	22.387563653305023
52-53	22.28414467220251	27.087560164863746	28.196731464576924	22.431563698356822
54-55	22.22810983535287	26.918406434601206	28.170576166822446	22.682907563223477
56-57	22.39024815334122	26.928623711560036	28.131492244772915	22.54963589032583
58-59	21.9839630512463	27.023348362144116	28.534962015747595	22.45772657086199
60-61	22.25367412004643	27.120454386647904	28.336212605665366	22.289658887640304
62-63	21.862806058543605	27.68617272627378	28.310869357855378	22.140151857327236
64-65	21.586663190198127	27.79689866195592	28.175713917975436	22.440724229870515
66-67	21.688566451839993	28.15444568092651	27.784805947783582	22.372181919449922
68-69	21.781606992892787	28.170674134660356	27.602330236432255	22.4453886360146
70-71	21.84615816556449	27.620391778501986	27.68323351989967	22.85021653603386
72-73	21.83524692130203	27.31045378765497	27.63154264722743	23.222756643815572
74-75	22.06506691824869	27.126373568911056	27.68392789674336	23.124631616096895
76-77	22.404430036710067	26.984075224770827	27.56423679004829	23.04725794847082
78-79	22.519062993426036	26.957586879972716	27.090341455807465	23.433008670793782
80-81	22.49002446341403	27.256398444850362	26.995246495577145	23.258330596158462
82-83	22.46915481698287	27.339580822507546	27.089651782305197	23.10161257820439
84-85	22.51426541431159	27.353721058248393	27.016625630604096	23.11538789683592
86-87	22.657508834357163	27.5843028420249	26.966303539401377	22.79188478421656
88-89	22.776502543657134	27.40076443721452	27.189627616985568	22.63310540214278
90-91	22.661096771692637	27.394200913767936	27.361678691591123	22.583023622948303
92-93	22.506126674278796	27.063087876354526	27.258008325735894	23.17277712363078
94-95	22.354420345353052	27.13434660055427	27.667753626049123	22.843479428043555
96-97	22.13160031206347	27.295148911117195	27.820092738350553	22.753158038468783
98-99	22.424203772675522	27.57235026226597	28.061888345324082	21.941557619734425
100-101	23.193314011019677	30.291907176399796	25.395974750239752	21.118804062340775
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1965.0
1	1795.5
2	2246.5
3	3122.0
4	3861.0
5	5378.0
6	7053.5
7	9361.0
8	12198.0
9	13709.5
10	13306.0
11	12539.5
12	13390.5
13	16175.5
14	21985.5
15	29158.0
16	34655.0
17	37802.5
18	37526.5
19	34071.0
20	29874.5
21	26547.0
22	25670.5
23	28515.5
24	34696.5
25	44400.0
26	57729.0
27	72578.0
28	89495.5
29	108601.5
30	127005.0
31	147788.0
32	171269.5
33	195853.0
34	219883.0
35	241753.5
36	266491.5
37	290572.5
38	308727.0
39	322058.5
40	337025.5
41	352831.0
42	362632.0
43	372743.0
44	385217.0
45	406885.0
46	419750.0
47	414068.5
48	407730.0
49	398655.5
50	388527.0
51	378277.5
52	363015.0
53	344369.5
54	325179.0
55	303378.5
56	278260.5
57	250480.0
58	223078.0
59	194159.5
60	161381.0
61	129858.0
62	101667.0
63	77147.0
64	57511.0
65	42546.0
66	31566.0
67	23882.0
68	18084.0
69	13250.0
70	9699.5
71	6996.0
72	4828.5
73	3198.5
74	2126.0
75	1336.5
76	821.0
77	493.0
78	314.0
79	199.5
80	122.5
81	87.5
82	67.0
83	42.5
84	29.5
85	30.0
86	28.0
87	23.5
88	19.5
89	12.5
90	8.5
91	5.5
92	3.0
93	1.5
94	0.5
95	0.5
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0015735716162464642
4	0.0
5	0.06650410567610057
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	5.17622242186337E-6
14-15	0.0
16-17	0.0
18-19	2.070488968745348E-5
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	8.966535097708332E-5
44-45	0.0
46-47	0.003203935971261976
48-49	3.4939915440029265E-4
50-51	6.49019501900248E-5
52-53	8.094806372231577E-4
54-55	9.731047968630145E-4
56-57	0.0019963186144091017
58-59	0.005464591515252851
60-61	0.003125455113719112
62-63	0.006023760302442187
64-65	0.004202553106308834
66-67	0.007923489756923951
68-69	0.002211581121808486
70-71	0.0026370443234296215
72-73	0.00347563656197602
74-75	0.003229800228478032
76-77	8.152032138571503E-4
78-79	3.816459308646635E-4
80-81	0.0
82-83	8.961448208265968E-5
84-85	0.001668970186950569
86-87	0.011449423478379824
88-89	0.002998977771748476
90-91	0.0025945273306827873
92-93	1.876035454318568E-4
94-95	9.221962596604006E-4
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	89.0
20-21	431.0
22-23	930.0
24-25	921.0
26-27	1740.0
28-29	4174.0
30-31	12196.0
32-33	16670.0
34-35	23179.0
36-37	36668.0
38-39	26946.0
40-41	44456.0
42-43	49812.0
44-45	61702.0
46-47	63008.0
48-49	58523.0
50-51	53072.0
52-53	6308.0
54-55	5407.0
56-57	5556.0
58-59	5824.0
60-61	60863.0
62-63	61781.0
64-65	74538.0
66-67	80917.0
68-69	78511.0
70-71	91274.0
72-73	64242.0
74-75	65667.0
76-77	70328.0
78-79	72795.0
80-81	73900.0
82-83	71200.0
84-85	70285.0
86-87	73828.0
88-89	77187.0
90-91	79784.0
92-93	78562.0
94-95	85797.0
96-97	145514.0
98-99	914504.0
100-101	6790465.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.38531205717132
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.77875555999499	75.76008304034683
2	7.037857114583579	11.877835376558373
3	1.6543307417755737	4.188036476715104
4	0.6687261047498277	2.2572264412036342
5	0.313075454058436	1.3209484944080865
6	0.16680470539388684	0.8445520270360601
7	0.10264650896303112	0.6063300383297231
8	0.07107818661820833	0.47983639665882954
9	0.04860612579092116	0.36914787834813045
>10	0.1543974736552004	1.9779776725052507
>50	0.0030268502671422825	0.16997830777101733
>100	6.117876092272446E-4	0.0810427858372482
>500	6.254053554862186E-5	0.04061861551344745
>1k	2.084600437763877E-5	0.026386448768283107
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.0352444843726739E-5	0.0	0.0	0.0
3	0.0	1.0352444843726739E-5	0.0	1.0352444843726739E-5	0.0
4	0.0	5.1762224218633694E-5	0.0	2.0704889687453477E-5	0.0
5	0.0	6.211466906236044E-5	0.0	2.0704889687453477E-5	0.0
6	3.105733453118022E-5	6.211466906236044E-5	0.0	3.105733453118022E-5	0.0
7	3.105733453118022E-5	6.211466906236044E-5	0.0	3.105733453118022E-5	0.0
8	3.105733453118022E-5	8.281955874981391E-5	0.0	3.105733453118022E-5	0.0
9	3.105733453118022E-5	1.4493422781217436E-4	0.0	3.105733453118022E-5	0.0
10-11	3.6233556953043584E-5	1.4493422781217436E-4	0.0	3.6233556953043584E-5	0.0
12-13	4.1409779374906954E-5	1.4493422781217436E-4	0.0	4.1409779374906954E-5	5.176222421863369E-6
14-15	5.1762224218633694E-5	2.1222511929639814E-4	0.0	4.1409779374906954E-5	3.6233556953043584E-5
16-17	7.246711390608718E-5	2.2775378656198826E-4	0.0	4.1409779374906954E-5	5.1762224218633694E-5
18-19	7.246711390608718E-5	2.4845867624944176E-4	0.0	5.17622242186337E-5	5.1762224218633694E-5
20-21	7.246711390608718E-5	2.691635659368952E-4	0.0	7.246711390608718E-5	5.1762224218633694E-5
22-23	7.764333632795054E-5	3.0022090046807545E-4	0.0	8.281955874981391E-5	7.764333632795054E-5
24-25	8.799578117167728E-5	3.1574956773366557E-4	0.0	9.834822601540401E-5	9.317200359354065E-5
26-27	9.317200359354065E-5	3.2610201257739224E-4	0.0	1.2940556054658425E-4	9.317200359354065E-5
28-29	9.317200359354065E-5	3.3127823499925563E-4	0.0	3.5198312468670914E-4	9.834822601540401E-5
30-31	9.317200359354065E-5	3.5198312468670914E-4	0.0	0.0014182849435905632	1.0870067085913077E-4
32-33	9.317200359354065E-5	3.571593471085725E-4	0.0	0.003788994812803987	1.2422933812472088E-4
34-35	1.0352444843726739E-4	4.296264610146597E-4	0.0	0.007314002282092941	1.2422933812472088E-4
36-37	1.0352444843726739E-4	4.969173524988835E-4	0.0	0.013002670723720786	1.345817829684476E-4
38-39	1.0352444843726739E-4	5.020935749207469E-4	0.0	0.031155682757195623	1.4493422781217436E-4
40-41	1.0870067085913077E-4	5.590320215612439E-4	0.0	0.24613455238202508	1.4493422781217436E-4
42-43	1.1387689328099413E-4	5.590320215612439E-4	0.0	0.26452567064690563	1.5011045023403773E-4
44-45	1.1387689328099413E-4	6.211466906236044E-4	0.0	0.29645778676738077	1.552866726559011E-4
46-47	1.1387689328099413E-4	6.832613596859648E-4	0.0	0.33325037574198557	1.7599156234335457E-4
48-49	1.345817829684476E-4	6.936138045296915E-4	0.0	0.3684952742124533	1.863440071870813E-4
50-51	1.39758005390311E-4	7.764333632795054E-4	0.0	0.40316043577167227	1.863440071870813E-4
52-53	1.4493422781217436E-4	7.867858081232322E-4	0.0	0.5483948844843147	1.863440071870813E-4
54-55	1.4493422781217436E-4	8.023144753888223E-4	0.0	1.057248605888015	1.9669645203080802E-4
56-57	1.4493422781217436E-4	8.385480323418659E-4	0.0	1.6181803010780829	2.0704889687453478E-4
58-59	1.4493422781217436E-4	8.54076699607456E-4	0.0	2.204227027459032	2.0704889687453478E-4
60-61	1.4493422781217436E-4	8.954864789823629E-4	0.0	2.6417109941100803	2.0704889687453478E-4
62-63	1.552866726559011E-4	9.213675910916798E-4	0.0	2.6492682788460007	2.0704889687453478E-4
64-65	1.552866726559011E-4	0.0010559493740601273	0.0	2.6543772103763796	2.0704889687453478E-4
66-67	1.7081533992149118E-4	0.0010714780413257174	0.0	2.6592428594529314	2.0704889687453478E-4
68-69	1.7599156234335457E-4	0.0011232402655443512	0.0	2.664103332307061	2.0704889687453478E-4
70-71	2.0187267445267141E-4	0.0011387689328099413	0.0	2.6687049940400973	2.0704889687453478E-4
72-73	2.2775378656198826E-4	0.0011387689328099413	0.0	2.6722765875111834	2.38106231405715E-4
74-75	2.2775378656198826E-4	0.0011542976000755315	0.0	2.6753253825176606	2.38106231405715E-4
76-77	2.2775378656198826E-4	0.001206059824294165	0.0	2.678001489509764	2.38106231405715E-4
78-79	2.2775378656198826E-4	0.0012215884915597552	0.0	2.6802272651511654	2.38106231405715E-4
80-81	2.2775378656198826E-4	0.0012267647139816186	0.0	2.6814229725306156	2.588111210931685E-4
82-83	2.2775378656198826E-4	0.001231940936403482	0.0	2.681873303881318	2.7951601078062194E-4
84-85	2.2775378656198826E-4	0.001247469603669072	0.0	2.682199405893895	2.7951601078062194E-4
86-87	2.2775378656198826E-4	0.0012629982709346623	0.0	2.6823495163441295	2.8469223320248534E-4
88-89	2.38106231405715E-4	0.0013354653848407494	0.0	2.6824116310131916	2.898684556243487E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11795	0.0	51.34967	1
GTATCAA	22400	0.0	41.043526	1
TCAACGC	27380	0.0	33.31888	4
ATCAACG	27410	0.0	33.039764	3
CAACGCA	28125	0.0	32.404747	5
TATCAAC	28345	0.0	32.3256	2
AACGCAG	29150	0.0	31.365303	6
ACGCAGA	33605	0.0	27.15444	7
CGCAGAG	34260	0.0	26.674116	8
GTGGTAT	5805	0.0	24.918297	1
TGGTATC	5740	0.0	23.422525	2
GCAGAGT	40015	0.0	22.76025	9
GAGTACT	22840	0.0	20.50063	12-13
GTACATG	29245	0.0	20.376394	1
TACATGG	29220	0.0	19.801605	2
AGAGTAC	35840	0.0	19.726767	10-11
CAGAGTA	38775	0.0	19.440012	10-11
ACATGGG	29505	0.0	18.918556	3
GTACTTT	25295	0.0	18.362091	14-15
AGTACTT	24925	0.0	17.905153	12-13
>>END_MODULE