FastQCFastQC Report
Fri 27 May 2016
SRR2140346_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140346_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11120364
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA152300.052.565541
GTATCAA282000.039.6255041
TCAACGC330400.033.8337364
ATCAACG331700.033.5414853
CAACGCA338800.032.981825
TATCAAC338850.032.6117062
AACGCAG351200.031.9124896
ACGCAGA408650.027.3178447
CGCAGAG419800.026.6133468
GTGGTAT71950.026.4484441
TGGTATC68850.025.7109762
GCAGAGT493800.022.5445049
GAGTACT279000.021.23484212-13
TACATGG380150.020.302712
GTACATG386950.020.2318441
CTTATAC101500.020.1872631
CAGAGTA478000.019.84768710-11
ACATGGG386100.019.749133
GTACTTT303000.019.35611714-15
TATACAC136400.018.4612183
Produced by FastQC (version 0.11.5)