##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140346_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11120364
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.80086299333367	32.0	18.0	33.0	18.0	33.0
2	27.721734558329207	29.0	25.0	32.0	18.0	33.0
3	27.198927031525226	29.0	25.0	31.0	18.0	33.0
4	30.454322628288068	31.0	29.0	33.0	27.0	33.0
5	31.912760589491494	33.0	32.0	33.0	31.0	33.0
6	35.98927409210706	38.0	36.0	38.0	31.0	38.0
7	36.14162153325197	38.0	37.0	38.0	33.0	38.0
8	36.594526582043535	38.0	37.0	38.0	34.0	38.0
9	36.81383325222088	38.0	38.0	38.0	35.0	38.0
10-11	36.881617679061584	38.0	38.0	38.0	35.0	38.0
12-13	36.94421805797005	38.0	38.0	38.0	35.0	38.0
14-15	36.93940027502697	38.0	38.0	38.0	35.0	38.0
16-17	36.95835990620451	38.0	38.0	38.0	35.0	38.0
18-19	36.96878847670814	38.0	38.0	38.0	35.0	38.0
20-21	36.98043094849376	38.0	38.0	38.0	35.5	38.0
22-23	36.99088488881475	38.0	38.0	38.0	36.0	38.0
24-25	36.99488487644524	38.0	38.0	38.0	36.0	38.0
26-27	36.952286592623054	38.0	38.0	38.0	35.5	38.0
28-29	36.934282200473774	38.0	38.0	38.0	35.0	38.0
30-31	36.928711990108205	38.0	38.0	38.0	35.0	38.0
32-33	36.903759405206515	38.0	38.0	38.0	35.0	38.0
34-35	36.88406891909507	38.0	38.0	38.0	35.0	38.0
36-37	36.85665020332259	38.0	38.0	38.0	35.0	38.0
38-39	36.82659842541533	38.0	38.0	38.0	35.0	38.0
40-41	36.82833061372014	38.0	38.0	38.0	35.0	38.0
42-43	36.78825136337289	38.0	38.0	38.0	35.0	38.0
44-45	36.7470862241815	38.0	38.0	38.0	35.0	38.0
46-47	36.693499417683725	38.0	38.0	38.0	34.5	38.0
48-49	36.65335065775803	38.0	38.0	38.0	34.0	38.0
50-51	36.6224359576094	38.0	38.0	38.0	34.0	38.0
52-53	36.52754301867631	38.0	38.0	38.0	34.0	38.0
54-55	36.56253094164511	38.0	38.0	38.0	34.0	38.0
56-57	36.552021498185674	38.0	38.0	38.0	34.0	38.0
58-59	36.54056972597047	38.0	38.0	38.0	34.0	38.0
60-61	36.50769553406114	38.0	38.0	38.0	34.0	38.0
62-63	36.52305383388187	38.0	38.0	38.0	34.0	38.0
64-65	36.5273715244704	38.0	38.0	38.0	34.0	38.0
66-67	36.51989527797735	38.0	38.0	38.0	34.0	38.0
68-69	36.503058087993054	38.0	38.0	38.0	34.0	38.0
70-71	36.50134664543387	38.0	38.0	38.0	34.0	38.0
72-73	36.488820087417736	38.0	38.0	38.0	34.0	38.0
74-75	36.482387909810164	38.0	38.0	38.0	34.0	38.0
76-77	36.46950729295814	38.0	38.0	38.0	34.0	38.0
78-79	36.454422377183235	38.0	38.0	38.0	34.0	38.0
80-81	36.440217531668594	38.0	38.0	38.0	34.0	38.0
82-83	36.426286123077816	38.0	38.0	38.0	34.0	38.0
84-85	36.423985544534084	38.0	38.0	38.0	34.0	38.0
86-87	36.40495491062639	38.0	38.0	38.0	34.0	38.0
88-89	36.37973244628522	38.0	38.0	38.0	34.0	38.0
90-91	36.37357515114583	38.0	38.0	38.0	34.0	38.0
92-93	36.3793663355891	38.0	38.0	38.0	34.0	38.0
94-95	36.360067272029994	38.0	38.0	38.0	34.0	38.0
96-97	36.35839879792235	38.0	38.0	38.0	34.0	38.0
98-99	36.37626743474772	38.0	38.0	38.0	34.0	38.0
100-101	35.838483534298895	38.0	36.5	38.0	31.5	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	11.0
19	54.0
20	167.0
21	558.0
22	2065.0
23	6214.0
24	14316.0
25	26944.0
26	45090.0
27	68817.0
28	98989.0
29	133181.0
30	172563.0
31	217726.0
32	276548.0
33	361677.0
34	509731.0
35	827134.0
36	2017978.0
37	6340599.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.34470274534179	19.016778587463502	13.620426453666445	22.018092213528263
2	14.992431902408951	20.402902279098058	40.21221787344371	24.39244794504928
3	20.068748392573553	25.596892518700106	29.366282802740223	24.96807628598612
4	13.061793660711107	17.89087119810107	39.14912317618381	29.898211965004023
5	13.879217666078342	38.21195727525327	34.2027491974737	13.70607586119469
6	30.01999754684289	37.512656959790164	18.659497117180695	13.807848376186246
7	26.816109616555718	33.03073532485088	22.40949127204829	17.743663786545117
8	24.598987946797422	36.48261873442272	20.882535859437695	18.035857459342157
9	25.88467427864771	17.208546410890865	20.923298913596714	35.98348039686471
10-11	24.53586051679603	26.71503828471802	28.32209898884605	20.427002209639898
12-13	25.41133545628542	24.175044989534516	28.553692127344032	21.85992742683603
14-15	22.602600958026194	25.514555998346815	26.241708454867123	25.64113458875986
16-17	22.011871375062128	28.747289994040663	26.90319101993343	22.337647610963778
18-19	22.04115350900384	27.74181672470434	28.77502930659464	21.442000459697184
20-21	22.895907724328545	26.86110336117092	28.711337946427467	21.53165096807307
22-23	22.551497589685006	26.82620380177132	28.66281049842075	21.959488110122926
24-25	22.361564398276634	26.96959825328532	28.823153143421763	21.845684205016283
26-27	22.281205200482585	27.133881056332566	28.76572080069352	21.819192942491334
28-29	22.216220561641165	27.14695236630401	28.773541341182046	21.86328573087277
30-31	22.482656621129784	27.05157922721593	28.725354720342093	21.740409431312187
32-33	22.302016860181812	27.19468871504746	28.64957138289937	21.853723041871362
34-35	22.433640411898605	27.101000713366503	28.725994431505956	21.739364443228936
36-37	22.276249922872	27.12245952370185	28.687921354837997	21.91336919858815
38-39	22.123270928198686	27.152825359254702	28.788729365557366	21.935174346989246
40-41	22.382835452955202	26.979406953708086	28.710272263605013	21.927485329731702
42-43	22.36632690077013	26.944617886897348	28.610475223357263	22.078579988975264
44-45	22.271044875486872	27.175630270184286	28.478902812333207	22.074422041995632
46-47	22.453644890799925	27.041478522266054	28.160553495225432	22.344323091708585
48-49	22.308505827444485	27.168884001501304	28.24088668010823	22.281723490945986
50-51	22.23997232560547	27.3348369929548	28.19401676361808	22.23117391782165
52-53	22.428355792010624	27.272627795185468	28.087435905429942	22.211580507373966
54-55	22.390933884479267	27.192057257601576	28.07067832353965	22.34633053437951
56-57	22.43393909618529	27.43630766627264	27.917325350531936	22.21242788701013
58-59	22.251494301851558	27.576218159927517	28.107316326415265	22.06497121180566
60-61	22.401870853322674	27.67459979924858	27.91872385963859	22.00480548779016
62-63	22.249742785413186	27.82150563089821	27.86470352777331	22.064048055915293
64-65	22.21001464860063	27.769796277884957	27.851552393194094	22.168636680320322
66-67	22.341178844114186	27.763790442231723	27.69525842015194	22.19977229350215
68-69	22.2681732459968	27.622286478634322	27.844906335119685	22.264633940249194
70-71	22.304797298595478	27.29618261678227	27.957785189634038	22.441234894988213
72-73	22.395607311050945	27.3117569518776	27.868169649578324	22.424466087493133
74-75	22.210404281385653	27.458174013414382	27.891414528053254	22.440007177146708
76-77	22.43408617792176	27.352100835539666	27.783319661804985	22.43049332473359
78-79	22.414923199560402	27.45251744699166	27.67276351394917	22.459795839498764
80-81	22.46510037006106	27.41883135694297	27.657341130743045	22.458727142252926
82-83	22.448559435857916	27.399900601671128	27.7954606984294	22.35607926404155
84-85	22.623271309474653	27.381332771353573	27.669790385366028	22.325605533805746
86-87	22.402971940239347	27.45749470748338	27.735153320998595	22.404380031278677
88-89	22.46258814419138	27.477268793119602	27.698940615515767	22.361202447173252
90-91	22.52509585974487	27.451619668702303	27.739848758840523	22.2834357127123
92-93	22.600864508721262	27.398261361702765	27.710054151512825	22.290819978063148
94-95	22.418886571044062	27.558820699837195	27.697979048231407	22.324313680887332
96-97	22.461787154468386	27.563298724731467	27.71503770135495	22.259876419445202
98-99	22.63096230799398	28.166812370478773	27.752184859011543	21.45004046251571
100-101	23.336952648362587	30.666531711306895	25.088746784108274	20.90776885622225
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2274.0
1	1942.0
2	2515.0
3	3834.5
4	4663.0
5	6295.5
6	8417.5
7	11087.5
8	15221.0
9	18290.5
10	17522.5
11	15709.0
12	16401.0
13	19557.5
14	25792.0
15	33141.5
16	39351.5
17	43667.0
18	43960.0
19	40678.0
20	36264.0
21	32454.0
22	31399.5
23	34735.5
24	42407.0
25	54771.5
26	71238.5
27	89736.0
28	109556.0
29	130960.0
30	151787.0
31	174667.0
32	201468.5
33	230402.0
34	259314.5
35	285678.0
36	314321.0
37	340563.0
38	359308.5
39	374494.5
40	393809.5
41	410593.0
42	418919.5
43	430291.0
44	454847.0
45	472270.5
46	469200.5
47	463366.5
48	456344.5
49	447221.0
50	438102.5
51	427802.0
52	411565.0
53	391394.0
54	370700.0
55	346938.5
56	320471.0
57	290971.0
58	261319.5
59	230219.5
60	193021.0
61	155816.0
62	121846.0
63	92970.0
64	70276.0
65	52061.0
66	38683.0
67	29541.5
68	22447.5
69	16379.0
70	11764.5
71	8253.0
72	5578.0
73	3638.5
74	2331.5
75	1451.5
76	906.5
77	547.5
78	352.5
79	230.0
80	149.0
81	100.0
82	73.0
83	56.0
84	42.0
85	38.0
86	33.5
87	26.5
88	22.0
89	16.5
90	10.0
91	5.0
92	2.5
93	1.0
94	0.5
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	9.35221185205808E-4
4	0.0
5	0.05193175331311097
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	8.992511396209692E-6
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	4.563495078658455E-6
42-43	7.792299109143112E-5
44-45	0.0
46-47	0.0031763755560975746
48-49	2.6595629274285066E-4
50-51	9.384967421962589E-6
52-53	8.489821576494779E-4
54-55	0.0010284589765013237
56-57	0.001958087587782829
58-59	0.005153655570277853
60-61	0.0030353179478687514
62-63	0.0056223903747778785
64-65	0.004052769694997624
66-67	0.007574250867539075
68-69	0.002054240157108287
70-71	0.0025400502041671397
72-73	0.0032634143047265657
74-75	0.003138005165055276
76-77	8.408734626603513E-4
78-79	3.696165351653172E-4
80-81	0.0
82-83	5.7347161993220526E-5
84-85	0.0012606565530844877
86-87	0.008622490684507725
88-89	0.002198420466910357
90-91	0.0019158934610394723
92-93	1.1944613478823069E-4
94-95	7.231798877098866E-4
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	127.0
20-21	453.0
22-23	1126.0
24-25	1045.0
26-27	2113.0
28-29	4798.0
30-31	13566.0
32-33	18063.0
34-35	24904.0
36-37	39674.0
38-39	46519.0
40-41	47146.0
42-43	54276.0
44-45	66291.0
46-47	68334.0
48-49	62292.0
50-51	56344.0
52-53	50434.0
54-55	53960.0
56-57	54840.0
58-59	60240.0
60-61	62002.0
62-63	63114.0
64-65	72287.0
66-67	77101.0
68-69	75483.0
70-71	83846.0
72-73	64915.0
74-75	66472.0
76-77	70391.0
78-79	73759.0
80-81	75285.0
82-83	72419.0
84-85	72250.0
86-87	74608.0
88-89	79051.0
90-91	81515.0
92-93	81805.0
94-95	89181.0
96-97	156417.0
98-99	972401.0
100-101	7929517.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.89962961113447
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.95668850372793	76.372895350107
2	6.9588586016786165	11.816090355975433
3	1.6791939724874427	4.276888389283002
4	0.6176274460161355	2.0974536561776342
5	0.2959292461789297	1.2562141695846682
6	0.15904701275713515	0.8101819484302892
7	0.09716662097847327	0.5774587092146508
8	0.06286198431742336	0.426956734813615
9	0.040016803491991146	0.3057670615222441
>10	0.12906498669069003	1.6806946881148044
>50	0.0025665694218155646	0.1475661883225432
>100	8.711584115065805E-4	0.11641037120769472
>500	5.3549730803297616E-5	0.030505428922444358
>1k	5.3544111127438504E-5	0.08491694832394943
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.992511396209692E-6
2	0.0	2.6977534188629077E-5	0.0	8.992511396209692E-6	8.992511396209692E-6
3	0.0	2.6977534188629077E-5	0.0	8.992511396209692E-6	8.992511396209692E-6
4	0.0	5.3955068377258155E-5	0.0	8.992511396209692E-6	8.992511396209692E-6
5	0.0	5.3955068377258155E-5	0.0	1.7985022792419385E-5	8.992511396209692E-6
6	4.496255698104846E-5	5.3955068377258155E-5	0.0	1.7985022792419385E-5	8.992511396209692E-6
7	4.496255698104846E-5	5.3955068377258155E-5	0.0	1.7985022792419385E-5	8.992511396209692E-6
8	4.496255698104846E-5	6.294757977346785E-5	0.0	1.7985022792419385E-5	8.992511396209692E-6
9	4.496255698104846E-5	1.16902648150726E-4	0.0	1.7985022792419385E-5	8.992511396209692E-6
10-11	4.496255698104846E-5	1.16902648150726E-4	0.0	2.248127849052423E-5	8.992511396209692E-6
12-13	4.496255698104846E-5	1.16902648150726E-4	0.0	2.6977534188629077E-5	8.992511396209692E-6
14-15	4.496255698104846E-5	2.3830155199955686E-4	0.0	2.6977534188629077E-5	1.3488767094314539E-5
16-17	7.194009116967754E-5	2.517903190938714E-4	0.0	2.6977534188629077E-5	2.6977534188629077E-5
18-19	7.194009116967754E-5	2.7876785328250045E-4	0.0	3.597004558483877E-5	3.597004558483877E-5
20-21	7.194009116967754E-5	3.102416431692344E-4	0.0	3.597004558483877E-5	3.597004558483877E-5
22-23	7.194009116967754E-5	3.776854786408071E-4	0.0	3.597004558483877E-5	4.496255698104846E-5
24-25	7.194009116967754E-5	4.181517799237507E-4	0.0	4.496255698104846E-5	5.8451324075363E-5
26-27	7.194009116967754E-5	4.4512931411237977E-4	0.0	1.1690264815072599E-4	7.194009116967754E-5
28-29	7.194009116967754E-5	4.496255698104846E-4	0.0	3.2822666596165375E-4	7.194009116967754E-5
30-31	7.194009116967754E-5	5.125731495839524E-4	0.0	0.0012184852941864133	7.643634686778239E-5
32-33	7.194009116967754E-5	5.125731495839524E-4	0.0	0.0033991693077672636	9.891762535830662E-5
34-35	7.643634686778239E-5	6.024982635460494E-4	0.0	0.00613289277221501	9.891762535830662E-5
36-37	8.093260256588723E-5	6.879271218100415E-4	0.0	0.010435809475301348	9.891762535830662E-5
38-39	9.442136966020178E-5	6.969196332062512E-4	0.0	0.01844364087362608	1.0791013675451631E-4
40-41	9.891762535830662E-5	7.643634686778239E-4	0.0	0.03959852393320938	1.0791013675451631E-4
42-43	1.0791013675451631E-4	8.138222813569772E-4	0.0	0.04728712117696866	1.1240639245262116E-4
44-45	1.0791013675451631E-4	9.217324181114934E-4	0.0	0.05754308042434582	1.2139890384883084E-4
46-47	1.0791013675451631E-4	9.711912307906467E-4	0.0	0.0685544106290046	1.3039141524504055E-4
48-49	1.0791013675451631E-4	9.801837421868566E-4	0.0	0.07964667343622925	1.3488767094314537E-4
50-51	1.1240639245262116E-4	0.0011330564359224213	0.0	0.09013643797990786	1.3488767094314537E-4
52-53	1.16902648150726E-4	0.0011600339701110503	0.0	0.10718623958712142	1.4388018233935508E-4
54-55	1.16902648150726E-4	0.0011780189929034698	0.0	0.14608334763142644	1.573689494336696E-4
56-57	1.16902648150726E-4	0.0012049965270920988	0.0	0.1882447373125556	1.7085771652798414E-4
58-59	1.16902648150726E-4	0.0012229815498845183	0.0	0.23544193337556218	1.7985022792419385E-4
60-61	1.2139890384883084E-4	0.0012724403625636715	0.0	0.24931737845991372	1.7985022792419385E-4
62-63	1.3938392664125023E-4	0.0012814328739598813	0.0	0.26035118994306305	1.8884273932040353E-4
64-65	1.4388018233935508E-4	0.0014477943347897604	0.0	0.26656051906214584	1.9333899501850838E-4
66-67	1.4388018233935508E-4	0.0014522905904878654	0.0	0.2710073159475715	1.9783525071661323E-4
68-69	1.4388018233935508E-4	0.0014837643803745993	0.0	0.2752922476278654	1.9783525071661323E-4
70-71	1.483764380374599E-4	0.001492756891770809	0.0	0.2794692691714048	2.1582027350903262E-4
72-73	1.8884273932040353E-4	0.001492756891770809	0.0	0.2828324684335872	2.517903190938714E-4
74-75	1.8884273932040353E-4	0.0015152381702613332	0.0	0.2854133192042994	2.517903190938714E-4
76-77	1.8884273932040353E-4	0.0015826820057329059	0.0	0.2879941699750116	2.517903190938714E-4
78-79	1.8884273932040353E-4	0.001609659539921535	0.0	0.29034571170512047	2.517903190938714E-4
80-81	1.8884273932040353E-4	0.001609659539921535	0.0	0.2912989179131187	2.607828304900811E-4
82-83	1.8884273932040353E-4	0.00161415579561964	0.0	0.2916810996474576	2.7876785328250045E-4
84-85	1.8884273932040353E-4	0.0016186520513177447	0.0	0.29209925142738136	2.7876785328250045E-4
86-87	1.8884273932040353E-4	0.0016456295855063737	0.0	0.2922386353540226	2.7876785328250045E-4
88-89	1.8884273932040353E-4	0.0017355546994684706	0.0	0.2923105754451923	2.7876785328250045E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	15230	0.0	52.56554	1
GTATCAA	28200	0.0	39.625504	1
TCAACGC	33040	0.0	33.833736	4
ATCAACG	33170	0.0	33.541485	3
CAACGCA	33880	0.0	32.98182	5
TATCAAC	33885	0.0	32.611706	2
AACGCAG	35120	0.0	31.912489	6
ACGCAGA	40865	0.0	27.317844	7
CGCAGAG	41980	0.0	26.613346	8
GTGGTAT	7195	0.0	26.448444	1
TGGTATC	6885	0.0	25.710976	2
GCAGAGT	49380	0.0	22.544504	9
GAGTACT	27900	0.0	21.234842	12-13
TACATGG	38015	0.0	20.30271	2
GTACATG	38695	0.0	20.231844	1
CTTATAC	10150	0.0	20.187263	1
CAGAGTA	47800	0.0	19.847687	10-11
ACATGGG	38610	0.0	19.74913	3
GTACTTT	30300	0.0	19.356117	14-15
TATACAC	13640	0.0	18.461218	3
>>END_MODULE