FastQCFastQC Report
Fri 27 May 2016
SRR2140345_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140345_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11064103
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA135950.050.432591
GTATCAA247050.037.7941
TCAACGC273800.033.971444
ATCAACG274700.033.650583
CAACGCA281750.032.981385
AACGCAG292350.031.8914076
TATCAAC292250.031.4627912
ACGCAGA343250.027.1235147
CGCAGAG350300.026.640958
GTGGTAT71100.024.2796271
TGGTATC67900.023.2670972
GCAGAGT420950.022.1591389
GAGTACT252350.020.87249612-13
CAGAGTA410250.019.65033310-11
GTACATG311950.019.4893591
TACATGG308350.019.1412242
ACATGGG307800.018.7861523
GTACTTT283150.018.43004214-15
AGAGTAC375750.017.74206210-11
CATGGGG228000.016.6996944
Produced by FastQC (version 0.11.5)