##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140345_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11064103
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.409461571353773	32.0	18.0	33.0	18.0	33.0
2	27.331718893072487	29.0	25.0	32.0	18.0	33.0
3	26.82962622455702	29.0	25.0	31.0	18.0	33.0
4	30.29903626168339	31.0	29.0	33.0	27.0	33.0
5	31.8629439729547	33.0	32.0	33.0	30.0	33.0
6	35.964046791682975	38.0	36.0	38.0	31.0	38.0
7	36.12680910508516	38.0	37.0	38.0	33.0	38.0
8	36.58872843103503	38.0	37.0	38.0	34.0	38.0
9	36.81216461921947	38.0	38.0	38.0	35.0	38.0
10-11	36.87864958415517	38.0	38.0	38.0	35.0	38.0
12-13	36.94377411345502	38.0	38.0	38.0	35.0	38.0
14-15	36.93636185418737	38.0	38.0	38.0	35.0	38.0
16-17	36.95044008538243	38.0	38.0	38.0	35.0	38.0
18-19	36.96179740011459	38.0	38.0	38.0	35.0	38.0
20-21	36.97370114094953	38.0	38.0	38.0	35.0	38.0
22-23	36.98156493805709	38.0	38.0	38.0	35.5	38.0
24-25	36.984217102783774	38.0	38.0	38.0	36.0	38.0
26-27	36.94123417602397	38.0	38.0	38.0	35.0	38.0
28-29	36.925187179951905	38.0	38.0	38.0	35.0	38.0
30-31	36.92138577531743	38.0	38.0	38.0	35.0	38.0
32-33	36.899294122722196	38.0	38.0	38.0	35.0	38.0
34-35	36.88429843093859	38.0	38.0	38.0	35.0	38.0
36-37	36.85761428952078	38.0	38.0	38.0	35.0	38.0
38-39	36.83270066432435	38.0	38.0	38.0	35.0	38.0
40-41	36.82592391547247	38.0	38.0	38.0	35.0	38.0
42-43	36.78583173129221	38.0	38.0	38.0	35.0	38.0
44-45	36.749844454673806	38.0	38.0	38.0	35.0	38.0
46-47	36.70204891345193	38.0	38.0	38.0	34.5	38.0
48-49	36.66501170415664	38.0	38.0	38.0	34.0	38.0
50-51	36.63728108807296	38.0	38.0	38.0	34.0	38.0
52-53	36.53932699253147	38.0	38.0	38.0	34.0	38.0
54-55	36.57482299562205	38.0	38.0	38.0	34.0	38.0
56-57	36.56451376259885	38.0	38.0	38.0	34.0	38.0
58-59	36.551676735667854	38.0	38.0	38.0	34.0	38.0
60-61	36.51529100082303	38.0	38.0	38.0	34.0	38.0
62-63	36.53053691425765	38.0	38.0	38.0	34.0	38.0
64-65	36.53492417800659	38.0	38.0	38.0	34.0	38.0
66-67	36.527424122876084	38.0	38.0	38.0	34.0	38.0
68-69	36.50987891763418	38.0	38.0	38.0	34.0	38.0
70-71	36.50832889894262	38.0	38.0	38.0	34.0	38.0
72-73	36.49304312303197	38.0	38.0	38.0	34.0	38.0
74-75	36.48555713681815	38.0	38.0	38.0	34.0	38.0
76-77	36.46952162175451	38.0	38.0	38.0	34.0	38.0
78-79	36.455183347007974	38.0	38.0	38.0	34.0	38.0
80-81	36.4401802686481	38.0	38.0	38.0	34.0	38.0
82-83	36.42408978781252	38.0	38.0	38.0	34.0	38.0
84-85	36.420554463104985	38.0	38.0	38.0	34.0	38.0
86-87	36.39887384096309	38.0	38.0	38.0	34.0	38.0
88-89	36.371532054268016	38.0	38.0	38.0	34.0	38.0
90-91	36.36621979238195	38.0	38.0	38.0	34.0	38.0
92-93	36.369202540762174	38.0	38.0	38.0	34.0	38.0
94-95	36.35083861759912	38.0	38.0	38.0	34.0	38.0
96-97	36.347169563316655	38.0	37.5	38.0	34.0	38.0
98-99	36.36345345359382	38.0	37.5	38.0	34.0	38.0
100-101	35.8139865126639	38.0	36.0	38.0	31.5	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	9.0
19	32.0
20	120.0
21	485.0
22	2050.0
23	6292.0
24	14261.0
25	27004.0
26	45129.0
27	68016.0
28	97041.0
29	130583.0
30	168623.0
31	215064.0
32	275224.0
33	363300.0
34	511118.0
35	833321.0
36	2067105.0
37	6239325.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.51917132369429	18.964781871607666	13.684887062240833	21.831159742457206
2	14.885110885175237	20.33920870042515	40.35143201396444	24.42424840043517
3	20.2256034336899	25.294874844721843	29.38500812001718	25.094513601571073
4	13.058853483196966	17.947157577979887	39.564355104069435	29.42963383475371
5	13.889942861235308	38.15413251404416	34.49770551526347	13.458219109457067
6	30.02885096062464	37.77339202283276	18.742640049536778	13.45511696700582
7	26.739772758803852	32.91359453179349	22.698921006068	17.647711703334647
8	24.205812256086194	36.969910710339555	21.0125484189726	17.811728614601655
9	25.46707130257193	17.46717289237094	21.277893020337935	35.787862784719195
10-11	24.251107387557763	26.86700855912133	28.709051244371096	20.17283280894981
12-13	25.05469263979195	24.31040275022747	28.917540807420178	21.717363802560406
14-15	22.23487073466326	25.79252018893895	26.498158052216255	25.474451024181537
16-17	21.772898674731913	29.003535849862544	27.218298169910053	22.005267305495494
18-19	21.770395666056256	28.104849530052277	28.957367804692346	21.167386999199124
20-21	22.485339413871205	27.271318107615162	28.97004487599279	21.273297602520838
22-23	22.20398037201624	27.20829279421457	28.838177780621994	21.74954905314719
24-25	22.015586808116748	27.34650026756917	28.94559215950015	21.69232076481393
26-27	21.949231507900354	27.515577273791987	28.8587897066456	21.67640151166206
28-29	21.890298644413598	27.52118766388387	28.934491300978376	21.654022390724155
30-31	22.224972605862288	27.323197717253954	28.847778796053035	21.604050880830716
32-33	21.956076009241283	27.48528853102339	28.817264560789983	21.74137089894535
34-35	22.07053060603849	27.47487463799696	28.822902040381397	21.631692715583156
36-37	22.0197370210016	27.45450687777305	28.826801147312565	21.698954953912786
38-39	21.86560206349011	27.524583123976893	28.912062992672183	21.69775181986082
40-41	22.126448090170488	27.363998169475945	28.825874766333985	21.68367897401958
42-43	22.03678112631072	27.38694129657383	28.718475500572037	21.85780207654341
44-45	22.040672883659237	27.545576052002584	28.594258935817173	21.81949212852101
46-47	22.13813943376886	27.48670432089687	28.351506025021024	22.023650220313247
48-49	22.050935220696363	27.581732678988423	28.39711075125988	21.970221349055326
50-51	22.024067808729555	27.714762454933428	28.341248993293643	21.919920743043377
52-53	22.17930998618365	27.646873474617035	28.21493465894653	21.958881880252783
54-55	22.13602553759259	27.586024433230882	28.258217341708747	22.019732687467773
56-57	22.14380969623219	27.744799669690558	28.17913768151646	21.932252952560795
58-59	22.04047599494356	27.812438098237706	28.276636218819213	21.870449687999518
60-61	22.16322278275551	27.836210038712466	28.207453729937388	21.79311344859463
62-63	22.028565705132095	27.956126244756156	28.16499155265576	21.850316497455992
64-65	22.005082345770926	27.862579855753584	28.163721173730323	21.96861662474517
66-67	22.099153177871386	27.906283929689646	28.025915044063677	21.96864784837529
68-69	22.011167354529622	27.82275991662973	28.142291817024017	22.023780911816633
70-71	22.025435486672524	27.610113612851165	28.186982379657593	22.177468520818717
72-73	22.107700845461288	27.62895797194165	28.11390695892655	22.149434223670514
74-75	21.97265263624898	27.743987577707575	28.119869500412815	22.163490285630626
76-77	22.155264359569813	27.671028498964677	28.01909424729586	22.15461289416965
78-79	22.13713902375219	27.713125927593723	27.951750541824183	22.197984506829904
80-81	22.128598722156653	27.756694353737426	27.92793472214467	22.186772201961254
82-83	22.158734233334652	27.74837853976897	28.023581639656687	22.069305587239697
84-85	22.266982332934372	27.731719955929208	27.967169323933394	22.03412838720303
86-87	22.128817428495168	27.766530787003816	27.992265446968993	22.112386337532016
88-89	22.17357029849138	27.78796194261357	27.965546819179384	22.07292093971567
90-91	22.231226889254447	27.82105758122674	27.95098470518132	21.996730824337497
92-93	22.26843085444346	27.771208012619436	27.93822966688077	22.022131466056337
94-95	22.122826003325912	27.86278596821322	27.966343707431545	22.04804432102932
96-97	22.17990985059106	27.829090954069834	27.995241390537075	21.995757804802036
98-99	22.35252323206357	28.43588557315871	28.055369652073914	21.1562215427038
100-101	22.980778953768194	30.983494329272503	25.43944687992984	20.596279837029467
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1760.0
1	1547.5
2	2144.0
3	3271.0
4	3976.5
5	5375.0
6	7152.0
7	9424.5
8	12742.5
9	15122.0
10	14803.0
11	13841.5
12	14478.0
13	17087.0
14	22633.0
15	29231.5
16	34962.0
17	39342.0
18	40395.5
19	38058.0
20	34922.0
21	32513.5
22	32862.0
23	37527.5
24	46178.5
25	59443.5
26	76124.5
27	95055.5
28	117090.0
29	140991.5
30	164431.0
31	190046.0
32	218291.0
33	246707.0
34	274726.5
35	300224.0
36	327225.5
37	353327.0
38	373640.5
39	389441.0
40	405867.5
41	422902.0
42	433884.5
43	442477.0
44	456082.0
45	466512.5
46	466150.5
47	461808.5
48	453990.0
49	443246.5
50	432064.0
51	417101.0
52	396100.5
53	372763.5
54	348921.0
55	322374.5
56	294828.0
57	265174.5
58	234820.0
59	203599.5
60	168759.5
61	135507.0
62	106284.5
63	81462.5
64	61052.5
65	45107.5
66	33259.5
67	25216.5
68	19358.5
69	14310.0
70	10390.0
71	7556.0
72	5431.5
73	3713.0
74	2463.0
75	1602.0
76	1002.0
77	586.0
78	372.5
79	228.5
80	140.5
81	90.5
82	60.5
83	45.5
84	34.5
85	27.0
86	24.5
87	21.0
88	15.0
89	8.5
90	4.5
91	2.5
92	2.0
93	2.0
94	1.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	7.863267361122723E-4
4	0.0
5	0.048905907690844894
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	1.8076476692236145E-5
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	1.65614577063844E-4
44-45	0.0
46-47	0.0029906302113649995
48-49	2.947631398038646E-4
50-51	4.704235095842439E-6
52-53	7.564069440993994E-4
54-55	9.262302712952214E-4
56-57	0.001866740597869748
58-59	0.00488590570269187
60-61	0.0026982488895668033
62-63	0.005379133254627098
64-65	0.003779751072626452
66-67	0.007383302528609068
68-69	0.001905858321363553
70-71	0.002288970850779048
72-73	0.0031541382670752516
74-75	0.0027550636680048184
76-77	6.921771965479902E-4
78-79	3.178370511980637E-4
80-81	0.0
82-83	3.122871080230774E-5
84-85	0.0011797234791083556
86-87	0.008405038099229425
88-89	0.0021034740978199593
90-91	0.0020674922924155846
92-93	9.749916800709967E-5
94-95	6.775934067975734E-4
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	102.0
20-21	409.0
22-23	862.0
24-25	917.0
26-27	1871.0
28-29	4123.0
30-31	12143.0
32-33	16416.0
34-35	22974.0
36-37	36826.0
38-39	42874.0
40-41	44031.0
42-43	51213.0
44-45	62608.0
46-47	64934.0
48-49	59472.0
50-51	53894.0
52-53	49057.0
54-55	52825.0
56-57	54137.0
58-59	58620.0
60-61	60043.0
62-63	59327.0
64-65	66103.0
66-67	70573.0
68-69	70849.0
70-71	75375.0
72-73	64650.0
74-75	65619.0
76-77	69676.0
78-79	73268.0
80-81	73892.0
82-83	70818.0
84-85	70743.0
86-87	74406.0
88-89	77723.0
90-91	80234.0
92-93	79637.0
94-95	87866.0
96-97	155549.0
98-99	969774.0
100-101	7957670.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.02945508481942
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.02200677265982	76.54522181521189
2	6.884631902696714	11.707929982917301
3	1.699726970815847	4.335805743643267
4	0.6119063108603282	2.081202407016633
5	0.2886129196820296	1.2270299645500866
6	0.1628942082298733	0.831048345735554
7	0.09511898666440084	0.5661540923005947
8	0.06169666365539354	0.41968269529355967
9	0.03926809448016533	0.30050502082808966
>10	0.13104930465719206	1.6893753846146862
>50	0.002385843623306796	0.1365139068888526
>100	6.139157872333635E-4	0.0841985437089356
>500	7.048670718612635E-5	0.0464922316495639
>1k	1.761948064938094E-5	0.02883986564101685
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.8076476692236145E-5	0.0	9.038238346118072E-6	0.0
3	0.0	2.7114715038354215E-5	0.0	9.038238346118072E-6	0.0
4	0.0	7.230590676894458E-5	0.0	9.038238346118072E-6	0.0
5	0.0	8.134414511506265E-5	0.0	9.038238346118072E-6	0.0
6	0.0	8.134414511506265E-5	0.0	9.038238346118072E-6	9.038238346118072E-6
7	0.0	8.134414511506265E-5	0.0	2.7114715038354215E-5	9.038238346118072E-6
8	0.0	9.038238346118073E-5	0.0	2.7114715038354215E-5	9.038238346118072E-6
9	0.0	1.1749709849953493E-4	0.0	3.615295338447229E-5	9.038238346118072E-6
10-11	0.0	1.1749709849953493E-4	0.0	4.519119173059036E-5	9.038238346118072E-6
12-13	1.8076476692236145E-5	1.1749709849953493E-4	0.0	4.971031090364939E-5	1.3557357519177108E-5
14-15	2.2595595865295178E-5	1.7172652857624338E-4	0.0	5.874854924976747E-5	2.7114715038354215E-5
16-17	4.519119173059036E-5	1.7624564774930242E-4	0.0	6.32676684228265E-5	4.519119173059036E-5
18-19	4.519119173059036E-5	1.9432212444153854E-4	0.0	8.134414511506265E-5	7.230590676894458E-5
20-21	4.519119173059036E-5	2.0787948196071565E-4	0.0	9.038238346118073E-5	7.230590676894458E-5
22-23	4.519119173059036E-5	2.395133161721289E-4	0.0	1.1749709849953493E-4	8.134414511506265E-5
24-25	4.519119173059036E-5	2.892236270757783E-4	0.0	1.5365005188400723E-4	9.038238346118073E-5
26-27	4.519119173059036E-5	2.982618654218964E-4	0.0	1.8076476692236142E-4	9.038238346118073E-5
28-29	4.519119173059036E-5	2.982618654218964E-4	0.0	3.7508689136389996E-4	9.490150263423975E-5
30-31	4.519119173059036E-5	3.344148188063687E-4	0.0	0.0011026650782264048	9.942062180729879E-5
32-33	4.971031090364939E-5	3.344148188063687E-4	0.0	0.0026030126436820044	1.0845886015341686E-4
34-35	5.874854924976747E-5	3.9768248722919515E-4	0.0	0.004659211867423866	1.26535336845653E-4
36-37	6.32676684228265E-5	4.6998839399813974E-4	0.0	0.00787230559946884	1.26535336845653E-4
38-39	6.32676684228265E-5	4.8354575151731685E-4	0.0	0.014415990162058325	1.3105445601871205E-4
40-41	6.32676684228265E-5	5.603707774593205E-4	0.0	0.033125143538522736	1.3557357519177108E-4
42-43	6.32676684228265E-5	5.739281349784976E-4	0.0	0.03934345152065197	1.4009269436483012E-4
44-45	6.32676684228265E-5	6.100810883629699E-4	0.0	0.04768122639494589	1.4461181353788916E-4
46-47	6.32676684228265E-5	6.326766842282651E-4	0.0	0.05755550178807989	1.4461181353788916E-4
48-49	6.32676684228265E-5	6.326766842282651E-4	0.0	0.06725353153346458	1.626882902301253E-4
50-51	6.32676684228265E-5	7.682502594200361E-4	0.0	0.07656743614913925	1.626882902301253E-4
52-53	6.32676684228265E-5	7.953649744583903E-4	0.0	0.09261934745184494	1.7624564774930242E-4
54-55	6.32676684228265E-5	7.953649744583903E-4	0.0	0.12927392306452679	1.9432212444153854E-4
56-57	6.32676684228265E-5	8.044032128045084E-4	0.0	0.17055607671042108	1.9884124361459757E-4
58-59	6.32676684228265E-5	8.134414511506264E-4	0.0	0.21920891372757467	1.9884124361459757E-4
60-61	6.778678759588554E-5	8.405561661889807E-4	0.0	0.23238214611704178	1.9884124361459757E-4
62-63	8.134414511506265E-5	8.586326428812168E-4	0.0	0.2405843474161439	2.0787948196071565E-4
64-65	8.134414511506265E-5	9.490150263423975E-4	0.0	0.24698342016519548	2.0787948196071565E-4
66-67	8.134414511506265E-5	0.001003244456419106	0.0	0.25127658337960157	2.1239860113377468E-4
68-69	9.038238346118073E-5	0.0010258400522844013	0.0	0.25558330395152684	2.1691772030683372E-4
70-71	9.038238346118073E-5	0.0010439165289766374	0.0	0.25965051120728	2.1691772030683372E-4
72-73	1.0845886015341686E-4	0.0010484356481496964	0.0	0.26284100934345966	2.4403243534518794E-4
74-75	1.0845886015341686E-4	0.0010574738864958144	0.0	0.2656473823499293	2.4403243534518794E-4
76-77	1.0845886015341686E-4	0.0010755503631880506	0.0	0.2681690508484963	2.48551554518247E-4
78-79	1.0845886015341686E-4	0.0010800694823611097	0.0	0.27046476338841025	2.53070673691306E-4
80-81	1.0845886015341686E-4	0.0010845886015341687	0.0	0.27137310634219514	2.621089120374241E-4
82-83	1.0845886015341686E-4	0.0010845886015341687	0.0	0.27173915499521295	2.8018538872966024E-4
84-85	1.0845886015341686E-4	0.0010891077207072276	0.0	0.27208712717153843	2.8018538872966024E-4
86-87	1.0845886015341686E-4	0.0011117033165725229	0.0	0.2721549139591343	2.8018538872966024E-4
88-89	1.0845886015341686E-4	0.0011659327466492313	0.0	0.27217750955499964	2.892236270757783E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13595	0.0	50.43259	1
GTATCAA	24705	0.0	37.794	1
TCAACGC	27380	0.0	33.97144	4
ATCAACG	27470	0.0	33.65058	3
CAACGCA	28175	0.0	32.98138	5
AACGCAG	29235	0.0	31.891407	6
TATCAAC	29225	0.0	31.462791	2
ACGCAGA	34325	0.0	27.123514	7
CGCAGAG	35030	0.0	26.64095	8
GTGGTAT	7110	0.0	24.279627	1
TGGTATC	6790	0.0	23.267097	2
GCAGAGT	42095	0.0	22.159138	9
GAGTACT	25235	0.0	20.872496	12-13
CAGAGTA	41025	0.0	19.650333	10-11
GTACATG	31195	0.0	19.489359	1
TACATGG	30835	0.0	19.141224	2
ACATGGG	30780	0.0	18.786152	3
GTACTTT	28315	0.0	18.430042	14-15
AGAGTAC	37575	0.0	17.742062	10-11
CATGGGG	22800	0.0	16.699694	4
>>END_MODULE