FastQCFastQC Report
Fri 27 May 2016
SRR2140344_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140344_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9301501
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA134900.053.4962351
GTATCAA248500.038.5848241
TCAACGC288250.033.3403634
ATCAACG287500.033.1202853
CAACGCA296100.032.3968055
TATCAAC296250.031.8438842
AACGCAG304600.031.5487966
GTGGTAT57950.030.7138821
TGGTATC57000.029.2886962
ACGCAGA352900.027.1933147
CGCAGAG362850.026.4962888
GCAGAGT420450.022.7824089
GAGTACT246650.021.48499912-13
CAGAGTA406450.020.50449810-11
GTACATG310100.020.4092731
TACATGG307500.020.0073282
ACATGGG308250.019.7007483
GTACTTT274550.019.2937414-15
AGAGTAC378300.018.20881710-11
CATGGGG226200.017.6504844
Produced by FastQC (version 0.11.5)